##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4410
Sequences flagged as poor quality	0
Sequence length	56
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.12312925170068	39.0	38.0	40.0	35.0	40.0
2	38.14013605442177	39.0	38.0	40.0	35.0	40.0
3	37.959410430839	39.0	38.0	40.0	35.0	40.0
4	37.94625850340136	39.0	38.0	40.0	35.0	40.0
5	37.816326530612244	39.0	38.0	40.0	35.0	40.0
6	37.22675736961451	39.0	37.0	39.0	33.0	40.0
7	37.62199546485261	39.0	38.0	40.0	35.0	40.0
8	37.64829931972789	39.0	38.0	39.0	35.0	40.0
9	37.54512471655329	39.0	38.0	39.0	35.0	40.0
10	37.28616780045351	39.0	38.0	39.0	33.0	40.0
11	35.773922902494334	38.0	35.0	39.0	29.0	40.0
12	34.311791383219955	38.0	33.0	39.0	23.0	40.0
13	34.114285714285714	38.0	33.0	39.0	22.0	40.0
14	34.1609977324263	38.0	33.0	39.0	23.0	40.0
15	34.11791383219955	38.0	33.0	39.0	22.0	40.0
16	33.362585034013605	38.0	31.0	39.0	20.0	40.0
17	33.38934240362812	37.0	31.0	39.0	20.0	40.0
18	32.83151927437642	37.0	30.0	39.0	18.0	40.0
19	33.36349206349206	37.0	31.0	39.0	22.0	40.0
20	33.81587301587302	37.0	31.0	39.0	23.0	40.0
21	34.34285714285714	38.0	33.0	39.0	25.0	40.0
22	33.997732426303855	38.0	33.0	39.0	23.0	40.0
23	34.50249433106576	38.0	33.0	39.0	25.0	40.0
24	34.04353741496599	38.0	33.0	39.0	23.0	40.0
25	34.231972789115645	38.0	33.0	39.0	24.0	40.0
26	34.84308390022676	38.0	35.0	39.0	26.0	40.0
27	34.91111111111111	38.0	34.0	39.0	26.0	40.0
28	34.70929705215419	38.0	34.0	39.0	26.0	40.0
29	34.51156462585034	38.0	34.0	39.0	24.0	40.0
30	34.36485260770975	38.0	33.0	39.0	23.0	40.0
31	34.43809523809524	38.0	34.0	39.0	25.0	40.0
32	34.364172335600905	38.0	33.0	39.0	25.0	40.0
33	34.39523809523809	38.0	34.0	39.0	25.0	40.0
34	34.44625850340136	38.0	34.0	39.0	25.0	40.0
35	33.919274376417235	38.0	33.0	39.0	23.0	40.0
36	33.46077097505669	37.0	33.0	39.0	23.0	40.0
37	33.23945578231292	36.0	32.0	39.0	23.0	40.0
38	32.99682539682539	36.0	32.0	39.0	20.0	40.0
39	32.38231292517007	36.0	31.0	38.0	17.0	40.0
40	29.534920634920635	35.0	24.0	38.0	7.0	39.0
41	26.43219954648526	32.0	15.0	38.0	4.0	39.0
42	30.019501133786846	35.0	28.0	38.0	4.0	39.0
43	28.79092970521542	34.0	24.0	38.0	4.0	39.0
44	28.58140589569161	34.0	23.0	38.0	4.0	39.0
45	29.564399092970522	35.0	27.0	38.0	4.0	39.0
46	29.226984126984128	35.0	25.0	38.0	4.0	40.0
47	28.69909297052154	35.0	23.0	38.0	4.0	40.0
48	28.733560090702948	35.0	25.0	38.0	4.0	40.0
49	26.463492063492062	33.0	12.0	38.0	4.0	39.0
50	28.16984126984127	34.0	23.0	38.0	4.0	39.0
51	28.194784580498865	35.0	21.0	39.0	4.0	40.0
52	28.165532879818596	35.0	23.0	39.0	4.0	40.0
53	27.587528344671203	34.0	18.0	39.0	4.0	40.0
54	26.572108843537414	34.0	10.0	38.0	4.0	40.0
55	26.869614512471657	33.0	11.0	38.0	4.0	40.0
56	25.72766439909297	33.0	4.0	38.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.3164285714285668
1	2	-1.280297619047623
1	3	-0.9756746031746104
1	4	0.4567460317460217
1	5	0.361309523809517
1	6	-0.0071031746031735565
1	7	0.9999603174602996
1	8	0.44664682539681877
1	9	-1.5629960317460316
1	10	1.093948412698417
1	11	-1.4693055555555574
1	12	-14.561408730158721
1	13	-5.506369047619039
1	14	-3.6865277777777834
1	15	-2.5715277777777743
1	16	-24.977718253968256
1	17	-5.811666666666671
1	18	-13.930277777777771
1	19	-9.357757936507937
1	20	-4.950337301587304
1	21	-7.019742063492057
1	22	-3.1379365079365087
1	23	-6.063769841269838
1	24	-4.173293650793639
1	25	-4.438432539682541
1	26	3.421369047619052
1	27	3.9605357142857187
1	28	3.514107142857142
1	29	-0.22859126984127442
1	30	3.0499404761904714
1	31	4.366150793650796
1	32	3.9370436507936617
1	33	1.867202380952378
1	34	3.227361111111108
1	35	0.8628571428571377
1	36	3.205773809523812
1	37	0.045873015873020506
1	38	4.745238095238086
1	39	3.7351388888888835
1	40	5.515535714285722
1	41	-6.68948412698413
1	42	-4.561309523809523
1	43	-12.29765873015873
1	44	-20.872083333333336
1	45	-6.09182539682541
1	46	-12.321646825396819
1	47	-22.497539682539685
1	48	-8.980773809523804
1	49	-19.013293650793653
1	50	-7.4131349206349135
1	51	-1.5111309523809595
1	52	-2.182797619047623
1	53	-0.9618055555555571
1	54	-0.490000000000002
1	55	-1.4577380952380992
1	56	2.5244246031746016
2	1	0.383571428571436
2	2	0.9197023809523799
2	3	1.2243253968253924
2	4	1.056746031746023
2	5	1.361309523809517
2	6	-0.0404365079365121
2	7	1.599960317460301
2	8	1.2799801587301545
2	9	1.6370039682539712
2	10	1.6939484126984183
2	11	2.2306944444444454
2	12	-1.7614087301587205
2	13	1.2269642857142955
2	14	3.980138888888881
2	15	4.428472222222226
2	16	-1.1443849206349235
2	17	3.654999999999994
2	18	2.8697222222222294
2	19	3.5422420634920613
2	20	1.9829960317460333
2	21	4.180257936507942
2	22	3.695396825396827
2	23	4.0028968253968245
2	24	4.993373015873026
2	25	3.9282341269841226
2	26	-0.04529761904760932
2	27	4.093869047619052
2	28	2.6141071428571436
2	29	2.9714087301587284
2	30	3.4166071428571385
2	31	-4.067182539682538
2	32	2.403710317460323
2	33	-1.79946428571429
2	34	-0.0393055555555577
2	35	0.9628571428571391
2	36	3.539107142857148
2	37	2.1125396825396834
2	38	4.411904761904751
2	39	4.335138888888885
2	40	5.515535714285722
2	41	-12.48948412698413
2	42	-9.294642857142854
2	43	-15.29765873015873
2	44	-14.972083333333337
2	45	-5.825158730158741
2	46	-6.6216468253968195
2	47	-8.964206349206354
2	48	-4.080773809523805
2	49	-7.3132936507936535
2	50	-2.97980158730158
2	51	7.622202380952377
2	52	7.183869047619041
2	53	6.271527777777777
2	54	8.976666666666663
2	55	8.475595238095238
2	56	1.5244246031746016
3	1	0.883571428571436
3	2	0.4197023809523799
3	3	1.2243253968253924
3	4	1.056746031746023
3	5	0.861309523809517
3	6	1.7928968253968236
3	7	1.2666269841269653
3	8	0.6133134920634831
3	9	1.6370039682539712
3	10	-0.972718253968246
3	11	3.3973611111111097
3	12	4.7385912698412795
3	13	3.89363095238096
3	14	3.980138888888881
3	15	-0.5715277777777743
3	16	3.6889484126984087
3	17	2.8216666666666654
3	18	1.3697222222222294
3	19	3.875575396825397
3	20	-0.6836706349206381
3	21	3.1802579365079424
3	22	2.195396825396827
3	23	2.5028968253968245
3	24	3.9933730158730256
3	25	4.4282341269841226
3	26	3.2880357142857193
3	27	2.5938690476190516
3	28	4.280773809523808
3	29	0.9714087301587284
3	30	2.58327380952381
3	31	1.7661507936507945
3	32	3.0703769841269946
3	33	0.3672023809523779
3	34	-1.3726388888888934
3	35	-0.7038095238095252
3	36	2.539107142857148
3	37	1.7792063492063548
3	38	2.7452380952380864
3	39	3.6684722222222206
3	40	5.015535714285722
3	41	-1.1561507936507986
3	42	4.038690476190478
3	43	-3.964325396825398
3	44	-5.80541666666667
3	45	0.34150793650792366
3	46	0.04501984126984837
3	47	-1.2975396825396857
3	48	0.4192261904761949
3	49	3.5200396825396787
3	50	2.52019841269842
3	51	2.288869047619041
3	52	0.8505357142857086
3	53	0.6048611111111093
3	54	5.143333333333331
3	55	2.6422619047619023
3	56	5.024424603174602
4	1	-3.116428571428564
4	2	0.06255952380952579
4	3	-0.34710317460317697
4	4	0.4853174603174537
4	5	1.218452380952371
4	6	-0.0642460317460305
4	7	0.3142460317460163
4	8	0.8752182539682494
4	9	0.4941468253968253
4	10	0.8368055555555642
4	11	2.4449801587301607
4	12	1.3814484126984254
4	13	3.1793452380952445
4	14	2.5991865079365013
4	15	0.4284722222222257
4	16	4.16513888888889
4	17	3.845476190476184
4	18	5.012579365079375
4	19	1.4708134920634919
4	20	-0.20748015873015646
4	21	1.7516865079365118
4	22	4.290634920634922
4	23	-0.37805555555555515
4	24	3.9695634920635072
4	25	2.4758531746031736
4	26	2.3356547619047703
4	27	3.0462500000000006
4	28	-0.6001785714285717
4	29	-0.4571626984127022
4	30	-0.7500595238095258
4	31	3.337579365079364
4	32	2.594186507936513
4	33	1.4386309523809473
4	34	1.341646825396822
4	35	-4.680000000000003
4	36	0.7772023809523816
4	37	3.588730158730165
4	38	2.8880952380952323
4	39	0.9065674603174543
4	40	6.086964285714291
4	41	10.653373015873015
4	42	6.324404761904763
4	43	9.845198412698416
4	44	6.242202380952378
4	45	5.079603174603161
4	46	7.592638888888896
4	47	9.41674603174603
4	48	5.562083333333341
4	49	8.329563492063492
4	50	5.329722222222234
4	51	7.0031547619047565
4	52	6.588630952380946
4	53	3.8667658730158685
4	54	0.3099999999999987
4	55	3.8565476190476176
4	56	5.0958531746031746
5	1	0.3121428571428666
5	2	0.20541666666666458
5	3	1.0814682539682465
5	4	0.3424603174603078
5	5	0.6470238095238017
5	6	-0.7785317460317458
5	7	0.17138888888887038
5	8	-0.5533531746031812
5	9	1.4941468253968253
5	10	-2.020337301587297
5	11	-0.5550198412698393
5	12	4.66716269841271
5	13	0.6079166666666751
5	14	1.3134722222222166
5	15	2.142757936507941
5	16	3.307996031746029
5	17	3.9883333333333297
5	18	0.4411507936507988
5	19	4.185099206349207
5	20	3.9353769841269823
5	21	2.0374007936507965
5	22	0.7192063492063454
5	23	4.33623015873016
5	24	0.9695634920635072
5	25	2.904424603174604
5	26	-0.23577380952379912
5	27	2.1891071428571465
5	28	-0.314464285714287
5	29	2.9714087301587284
5	30	2.249940476190474
5	31	2.48043650793651
5	32	3.451329365079374
5	33	0.724345238095232
5	34	1.9130753968253913
5	35	1.7485714285714238
5	36	3.0629166666666663
5	37	2.731587301587304
5	38	4.6023809523809405
5	39	4.0494246031746
5	40	6.801250000000007
5	41	9.081944444444439
5	42	6.895833333333332
5	43	8.416626984126985
5	44	6.956488095238093
5	45	3.6510317460317374
5	46	4.592638888888896
5	47	4.559603174603168
5	48	-0.15220238095237804
5	49	-0.9561507936507958
5	50	-1.0988492063491968
5	51	-2.139702380952386
5	52	-1.8399404761904812
5	53	-2.704662698412701
5	54	0.5957142857142834
5	55	-0.7148809523809554
5	56	-0.6184325396825407
6	1	1.3121428571428666
6	2	1.4911309523809493
6	3	1.7957539682539618
6	4	1.6281746031745925
6	5	1.9327380952380864
6	6	1.6500396825396777
6	7	1.3142460317460163
6	8	1.5895039682539647
6	9	1.7798611111111171
6	10	0.979662698412703
6	11	4.01640873015873
6	12	5.2385912698412795
6	13	5.036488095238106
6	14	4.027757936507932
6	15	3.999900793650795
6	16	5.307996031746029
6	17	5.41690476190476
6	18	4.441150793650799
6	19	6.327956349206346
6	20	4.649662698412698
6	21	4.180257936507942
6	22	5.576349206349207
6	23	5.193373015873014
6	24	5.398134920634931
6	25	5.475853174603174
6	26	4.478511904761909
6	27	4.1891071428571465
6	28	4.685535714285713
6	29	4.685694444444444
6	30	5.39279761904762
6	31	5.623293650793649
6	32	6.1656150793650895
6	33	6.152916666666663
6	34	5.7702182539682525
6	35	5.89142857142857
6	36	6.348630952380958
6	37	6.588730158730165
6	38	6.030952380952371
6	39	5.7637103174603155
6	40	8.801250000000007
6	41	3.510515873015869
6	42	6.038690476190478
6	43	8.27376984126984
6	44	7.527916666666663
6	45	7.793888888888876
6	46	8.592638888888896
6	47	8.84531746031746
6	48	5.276369047619049
6	49	8.186706349206347
6	50	4.6154365079365185
6	51	5.288869047619041
6	52	5.874345238095231
6	53	5.2953373015872955
6	54	1.452857142857141
6	55	0.7136904761904752
6	56	3.238710317460317
7	1	-1.4021428571428487
7	2	0.20541666666666458
7	3	0.08146825396824653
7	4	-2.3718253968254075
7	5	-2.2101190476190524
7	6	0.6500396825396777
7	7	-0.8286111111111296
7	8	-0.8390674603174659
7	9	-1.077281746031744
7	10	-0.8774801587301511
7	11	-1.9835912698412699
7	12	1.9528769841269948
7	13	2.465059523809529
7	14	2.3134722222222166
7	15	4.142757936507941
7	16	4.450853174603175
7	17	2.702619047619045
7	18	3.5840079365079447
7	19	3.8993849206349154
7	20	3.0782341269841282
7	21	2.0374007936507965
7	22	4.147777777777776
7	23	0.47908730158730606
7	24	3.3981349206349307
7	25	0.6187103174603124
7	26	1.1927976190476244
7	27	1.9033928571428618
7	28	0.5426785714285742
7	29	1.399980158730159
7	30	2.9642261904761895
7	31	0.051865079365079225
7	32	2.3084722222222283
7	33	0.4386309523809473
7	34	-0.5154960317460393
7	35	1.7485714285714238
7	36	0.4914880952380969
7	37	0.3030158730158732
7	38	-1.969047619047629
7	39	0.6208531746031696
7	40	2.6583928571428643
7	41	-1.6323412698412731
7	42	-2.2470238095238066
7	43	4.559484126984124
7	44	4.385059523809517
7	45	1.7938888888888762
7	46	3.44978174603175
7	47	3.559603174603172
7	48	3.13351190476191
7	49	7.615277777777777
7	50	3.0440079365079455
7	51	7.288869047619041
7	52	7.731488095238092
7	53	7.2953373015872955
7	54	7.881428571428568
7	55	9.142261904761902
7	56	8.667281746031748
8	1	1.4835714285714374
8	2	1.5197023809523813
8	3	1.8243253968253939
8	4	1.6567460317460245
8	5	1.9613095238095184
8	6	1.392896825396825
8	7	2.1999603174603024
8	8	2.04664682539682
8	9	2.2370039682539726
8	10	2.2939484126984198
8	11	3.130694444444444
8	12	5.838591269841281
8	13	4.89363095238096
8	14	5.513472222222219
8	15	4.828472222222224
8	16	6.422281746031743
8	17	4.588333333333331
8	18	7.069722222222232
8	19	5.642242063492063
8	20	6.049662698412696
8	21	4.580257936507941
8	22	5.862063492063491
8	23	5.33623015873016
8	24	6.22670634920636
8	25	6.161567460317457
8	26	4.821369047619058
8	27	4.960535714285719
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116	25	3.7615674603174583
116	26	2.871369047619055
116	27	3.260535714285716
116	28	2.3641071428571436
116	29	4.471408730158728
116	30	3.249940476190474
116	31	4.0161507936507945
116	32	4.487043650793659
116	33	1.617202380952378
116	34	3.6273611111111066
116	35	2.962857142857139
116	36	2.455773809523812
116	37	-3.054126984126981
116	38	2.9952380952380864
116	39	2.085138888888885
116	40	7.265535714285722
116	41	-1.4894841269841308
116	42	-4.961309523809522
116	43	-11.79765873015873
116	44	-3.9720833333333374
116	45	-5.2418253968254085
116	46	-5.1216468253968195
116	47	-3.7975396825396857
116	48	-3.830773809523805
116	49	-5.0632936507936535
116	50	-4.063134920634912
116	51	-2.461130952380959
116	52	-3.7327976190476235
116	53	-4.3118055555555586
116	54	-9.440000000000001
116	55	-3.6077380952380977
116	56	-3.4755753968253984
117	1	-2.116428571428564
117	2	-2.08029761904762
117	3	-1.2756746031746076
117	4	-1.443253968253977
117	5	-1.138690476190483
117	6	-0.7071031746031764
117	7	-3.650039682539699
117	8	-1.0533531746031812
117	9	-2.112996031746029
117	10	-6.056051587301582
117	11	-4.519305555555555
117	12	-3.7614087301587205
117	13	-0.6063690476190402
117	14	-13.186527777777783
117	15	-10.071527777777774
117	16	-21.477718253968256
117	17	-13.01166666666667
117	18	-21.63027777777777
117	19	-4.207757936507939
117	20	-5.850337301587302
117	21	-0.5697420634920576
117	22	-1.8879365079365087
117	23	-1.1637698412698398
117	24	-4.423293650793639
117	25	-1.2384325396825417
117	26	2.121369047619055
117	27	1.5105357142857159
117	28	0.8641071428571436
117	29	1.4714087301587284
117	30	-0.7500595238095258
117	31	-3.9838492063492055
117	32	-4.512956349206341
117	33	1.117202380952378
117	34	1.8773611111111066
117	35	0.7128571428571391
117	36	2.455773809523812
117	37	0.19587301587301909
117	38	-2.2547619047619136
117	39	-1.9148611111111151
117	40	7.265535714285722
117	41	1.0105158730158692
117	42	3.788690476190478
117	43	-8.79765873015873
117	44	-2.2220833333333374
117	45	0.2581746031745915
117	46	3.6283531746031805
117	47	0.20246031746031434
117	48	5.419226190476195
117	49	0.18670634920634654
117	50	5.186865079365088
117	51	2.288869047619041
117	52	-3.4827976190476235
117	53	2.1881944444444414
117	54	-5.190000000000001
117	55	-1.3577380952380977
117	56	-6.975575396825398
118	1	-0.4497619047618997
118	2	-0.4136309523809558
118	3	-0.10900793650794327
118	4	-0.2765873015873126
118	5	0.027976190476181273
118	6	0.4595634920634879
118	7	0.2666269841269653
118	8	0.11331349206348307
118	9	0.3036706349206355
118	10	0.36061507936508264
118	11	-3.9359722222222224
118	12	-4.428075396825388
118	13	-5.773035714285708
118	14	-20.686527777777783
118	15	-14.238194444444442
118	16	-13.977718253968256
118	17	-12.01166666666667
118	18	-11.463611111111103
118	19	-5.624424603174607
118	20	-4.350337301587302
118	21	3.513591269841278
118	22	3.5287301587301556
118	23	3.669563492063496
118	24	1.160039682539697
118	25	3.4282341269841226
118	26	2.621369047619055
118	27	2.42720238095238
118	28	3.780773809523808
118	29	3.304742063492064
118	30	2.58327380952381
118	31	4.09948412698413
118	32	-10.262956349206341
118	33	0.8672023809523779
118	34	1.9606944444444423
118	35	3.1295238095238034
118	36	4.8724404761904765
118	37	5.112539682539683
118	38	5.411904761904751
118	39	6.001805555555549
118	40	9.182202380952386
118	41	1.8438492063492014
118	42	1.3720238095238102
118	43	-19.964325396825394
118	44	-19.13875
118	45	0.8415079365079237
118	46	-7.1216468253968195
118	47	-8.297539682539686
118	48	-1.580773809523805
118	49	-18.813293650793653
118	50	-4.146468253968244
118	51	1.955535714285709
118	52	1.3505357142857086
118	53	1.7715277777777736
118	54	-9.690000000000001
118	55	3.80892857142857
118	56	7.857757936507937
119	1	-0.616428571428564
119	2	-0.5802976190476201
119	3	-0.27567460317460757
119	4	-0.4432539682539769
119	5	-0.638690476190483
119	6	0.0428968253968236
119	7	-0.900039682539699
119	8	-0.05335317460318123
119	9	-0.3629960317460288
119	10	-0.30605158730158166
119	11	-1.2693055555555546
119	12	-12.76140873015872
119	13	-10.10636904761904
119	14	-9.436527777777783
119	15	-10.821527777777774
119	16	-13.977718253968256
119	17	-13.26166666666667
119	18	-15.13027777777777
119	19	-7.707757936507939
119	20	-6.850337301587302
119	21	-0.3197420634920576
119	22	1.6120634920634913
119	23	1.8362301587301602
119	24	1.5767063492063613
119	25	2.0115674603174583
119	26	1.621369047619055
119	27	3.260535714285716
119	28	3.3641071428571436
119	29	2.4714087301587284
119	30	3.499940476190474
119	31	3.0161507936507945
119	32	-0.5129563492063411
119	33	4.117202380952378
119	34	1.8773611111111066
119	35	2.462857142857139
119	36	4.455773809523812
119	37	2.945873015873019
119	38	4.745238095238086
119	39	3.335138888888885
119	40	8.765535714285722
119	41	10.01051587301587
119	42	5.788690476190478
119	43	7.45234126984127
119	44	1.5279166666666626
119	45	3.7581746031745915
119	46	-6.6216468253968195
119	47	0.9524603174603143
119	48	-11.330773809523805
119	49	-2.8132936507936535
119	50	2.436865079365088
119	51	3.788869047619041
119	52	5.5172023809523765
119	53	-3.8118055555555586
119	54	-7.690000000000001
119	55	-4.107738095238098
119	56	-1.4755753968253984
120	1	-1.616428571428564
120	2	-1.5802976190476201
120	3	-1.2756746031746076
120	4	-1.443253968253977
120	5	-1.138690476190483
120	6	-0.7071031746031764
120	7	-0.900039682539699
120	8	-1.0533531746031812
120	9	-0.8629960317460288
120	10	-1.8060515873015817
120	11	-3.7693055555555546
120	12	-16.76140873015872
120	13	-27.10636904761904
120	14	-16.186527777777783
120	15	-14.071527777777774
120	16	-16.477718253968256
120	17	-17.01166666666667
120	18	-23.63027777777777
120	19	-5.457757936507939
120	20	-24.350337301587302
120	21	-6.319742063492058
120	22	-7.637936507936509
120	23	-7.66376984126984
120	24	-11.673293650793639
120	25	-4.738432539682542
120	26	1.121369047619055
120	27	1.2605357142857159
120	28	-4.885892857142856
120	29	0.9714087301587284
120	30	-1.7500595238095258
120	31	-7.7338492063492055
120	32	1.7370436507936589
120	33	-1.632797619047622
120	34	2.1273611111111066
120	35	1.962857142857139
120	36	1.2057738095238122
120	37	2.445873015873019
120	38	1.7452380952380864
120	39	-4.664861111111115
120	40	5.515535714285722
120	41	-6.989484126984131
120	42	-11.961309523809522
120	43	-8.79765873015873
120	44	-8.972083333333337
120	45	-10.991825396825408
120	46	-9.62164682539682
120	47	-8.797539682539686
120	48	-9.080773809523805
120	49	-15.313293650793653
120	50	-8.313134920634912
120	51	-7.711130952380959
120	52	-7.9827976190476235
120	53	-10.061805555555559
120	54	-8.190000000000001
120	55	-9.357738095238098
120	56	-5.975575396825398
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	12.0
5	0.0
6	2.0
7	4.0
8	8.0
9	17.0
10	13.0
11	8.0
12	11.0
13	3.0
14	10.0
15	10.0
16	10.0
17	26.0
18	24.0
19	34.0
20	42.0
21	43.0
22	58.0
23	75.0
24	66.0
25	102.0
26	117.0
27	111.0
28	118.0
29	151.0
30	167.0
31	242.0
32	248.0
33	256.0
34	291.0
35	366.0
36	432.0
37	509.0
38	500.0
39	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	97.46031746031746	1.6099773242630386	0.27210884353741494	0.6575963718820862
8	97.39229024943312	1.4965986394557822	0.5668934240362812	0.5442176870748299
9	89.31972789115646	6.848072562358276	2.1088435374149657	1.7233560090702946
10	34.807256235827666	43.01587301587302	8.231292517006802	13.945578231292515
11	29.024943310657598	38.25396825396825	15.124716553287982	17.596371882086167
12	42.131519274376416	19.160997732426306	16.825396825396826	21.882086167800455
13	24.01360544217687	19.523809523809526	19.024943310657598	37.43764172335601
14	21.859410430839002	30.657596371882086	20.294784580498867	27.188208616780045
15	27.113636363636363	20.522727272727273	24.113636363636363	28.249999999999996
16	23.434664246823957	25.45372050816697	32.23684210526316	18.87477313974592
17	35.147392290249435	24.53514739229025	17.64172335600907	22.67573696145125
18	31.859410430839002	26.05442176870748	18.934240362811792	23.151927437641724
19	26.254826254826252	29.230070406540992	23.64297070179423	20.87213263683852
20	24.642614023144997	34.53596550941684	21.12548218742909	19.695938280009077
21	21.79138321995465	22.993197278911566	32.04081632653061	23.174603174603174
22	26.961451247165535	26.485260770975056	20.181405895691608	26.3718820861678
23	21.496598639455783	37.02947845804989	21.950113378684808	19.523809523809526
24	21.002494896801995	37.53685643002949	19.66432297573146	21.796325697437062
25	22.244897959183675	32.72108843537415	20.38548752834467	24.648526077097504
26	24.308390022675738	26.3718820861678	27.596371882086167	21.723356009070294
27	24.96025437201908	25.618896207131503	26.004996593231887	23.415852827617535
28	24.630765735060216	34.26493978641218	25.33515110202227	15.76914337650534
29	23.95100929916081	29.689271943751415	26.400544341120437	19.95917441596734
30	18.54875283446712	34.78458049886621	28.730158730158728	17.936507936507937
31	21.65532879818594	29.38775510204082	34.399092970521544	14.557823129251702
32	18.157058556513846	29.255560599182935	36.26872446663641	16.31865637766682
33	19.5	30.727272727272727	32.15909090909091	17.613636363636363
34	18.87049217509639	29.417101383533684	35.019278748015424	16.6931276933545
35	14.201451905626133	34.5961887477314	38.225952813067146	12.976406533575318
36	16.742286751361164	30.104355716878402	36.68330308529946	16.47005444646098
37	16.40909090909091	29.59090909090909	40.56818181818182	13.431818181818182
38	16.689404274670306	30.05911778080946	38.926784902228285	14.32469304229195
39	15.8145875937287	28.334469438763914	39.83185639627357	16.01908657123381
40	13.720557969357419	21.518408415275555	36.29087582895038	28.47015778641665
41	12.657358663309681	19.95880064087892	31.494621194781413	35.889219501029984
42	14.05540417801998	29.836512261580385	43.66485013623978	12.443233424159855
43	14.415437003405222	23.927355278093078	40.045402951191825	21.611804767309874
44	15.177757520510482	25.752051048313586	37.55697356426618	21.513217866909752
45	16.083598364379828	29.781917310313492	40.75420263516583	13.380281690140844
46	15.709421112372304	30.14755959137344	39.364358683314414	14.778660612939841
47	14.334316171467453	30.437740984350192	39.124518031299615	16.103424812882743
48	14.986376021798364	31.539509536784742	40.14532243415077	13.328792007266122
49	12.115691186517877	25.552265998633565	36.347073559553635	25.98496925529492
50	13.254652746255108	30.526554698138902	41.625056740807985	14.593735814798004
51	15.237662042301569	34.31885376392995	37.5710711848988	12.872413008869685
52	14.66182478438493	34.15796640944167	37.7666817975488	13.413527008624603
53	15.22381276982504	33.17427857305158	39.19563735514656	12.406271301976822
54	12.125340599455042	31.902815622161672	37.03451407811081	18.93732970027248
55	14.126478616924476	32.620564149226574	38.2393084622384	15.013648771610555
56	16.90909090909091	30.363636363636363	37.34090909090909	15.386363636363637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	0.5
11	0.0
12	0.5
13	4.5
14	8.0
15	17.5
16	27.0
17	35.5
18	44.0
19	52.5
20	61.0
21	66.0
22	74.5
23	78.0
24	70.0
25	62.0
26	73.0
27	84.0
28	95.5
29	154.5
30	202.0
31	201.5
32	201.0
33	224.0
34	247.0
35	219.0
36	191.0
37	195.0
38	201.0
39	203.0
40	182.0
41	161.0
42	140.0
43	119.0
44	103.5
45	88.0
46	88.0
47	77.5
48	67.0
49	88.0
50	109.0
51	116.5
52	124.0
53	151.0
54	160.5
55	143.0
56	143.5
57	144.0
58	161.5
59	179.0
60	172.0
61	165.0
62	164.5
63	196.5
64	229.0
65	212.0
66	195.0
67	167.0
68	139.0
69	117.5
70	96.0
71	87.0
72	63.0
73	48.0
74	47.0
75	46.0
76	37.5
77	29.0
78	23.0
79	11.0
80	5.0
81	6.0
82	7.0
83	4.5
84	2.0
85	2.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.22675736961451248
16	0.045351473922902494
17	0.0
18	0.0
19	0.15873015873015872
20	0.06802721088435373
21	0.0
22	0.0
23	0.0
24	0.022675736961451247
25	0.0
26	0.0
27	0.15873015873015872
28	0.20408163265306123
29	0.022675736961451247
30	0.0
31	0.0
32	0.09070294784580499
33	0.22675736961451248
34	0.022675736961451247
35	0.045351473922902494
36	0.045351473922902494
37	0.22675736961451248
38	0.27210884353741494
39	0.20408163265306123
40	0.8390022675736961
41	0.9297052154195011
42	0.13605442176870747
43	0.11337868480725624
44	0.4988662131519274
45	0.18140589569160998
46	0.11337868480725624
47	0.022675736961451247
48	0.13605442176870747
49	0.43083900226757366
50	0.09070294784580499
51	0.29478458049886624
52	0.09070294784580499
53	0.20408163265306123
54	0.13605442176870747
55	0.31746031746031744
56	0.22675736961451248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	4410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.92743764172336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.70384721837807	73.4920634920635
2	6.6703570440077495	10.929705215419501
3	1.7713811237199002	4.353741496598639
4	0.7196235815112095	2.35827664399093
5	0.3044561306393579	1.2471655328798186
6	0.249100470523111	1.2244897959183674
7	0.11071132023249376	0.6349206349206349
8	0.02767783005812344	0.18140589569160998
9	0.11071132023249376	0.8163265306122449
>10	0.3321339606974813	4.761904761904762
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	42	0.9523809523809524	No Hit
GTCCCGGGGGGAGCTTCAGATGACATGACTGTGGTAGATGCAGCTTCATTAAGACG	24	0.5442176870748299	No Hit
GTCCCGAGGAATGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.4988662131519274	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT	18	0.40816326530612246	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTT	16	0.36281179138321995	No Hit
GTCCCGGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGTCTCTTAACCCAGAACT	15	0.3401360544217687	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	14	0.31746031746031744	No Hit
GTCCCGGGGAGAGACCCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.31746031746031744	No Hit
GTCCCGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.27210884353741494	No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	12	0.27210884353741494	No Hit
GTCCCGGGGGCTCTTGCTTGCTTTTCTCTCTCTCTTGCTTCTTGCTCTCTTTTCCT	11	0.2494331065759637	No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	10	0.22675736961451248	No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.20408163265306123	No Hit
GTCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.20408163265306123	No Hit
GTCCCGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC	9	0.20408163265306123	No Hit
GTCCCGGGATATCAAGACCCAGAAACTGAAGAAAAATCTTGTTTTCTCATGCATAC	9	0.20408163265306123	No Hit
GTCCCGGGAAGCATGGTTACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.18140589569160998	No Hit
GTCCCGGGGATGCCGCAGATCGACTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTT	7	0.15873015873015872	No Hit
GTCCCGGGGAAGTGGTCACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.15873015873015872	No Hit
GTCCCGAGGAATGGTCAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTTTT	7	0.15873015873015872	No Hit
GTCCCGGGCATGTATCAACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.15873015873015872	No Hit
GTCCCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.13605442176870747	No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTGATATG	6	0.13605442176870747	No Hit
GTCCCGGGGAAGCATGGTATCAACGCAGAATCGACTTTTTTTTTTTTTTTTTTTTT	6	0.13605442176870747	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCCTTTTTTTTTTTTTTT	6	0.13605442176870747	No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	6	0.13605442176870747	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	6	0.13605442176870747	No Hit
GTCCCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCCGGCAGCATTTGATATG	6	0.13605442176870747	No Hit
GTCCCGGGGGAGTCACGCAGAGTCGATTTTTTTGTTTTTTTTTTTTTTTTTTTTTT	6	0.13605442176870747	No Hit
GTCCCGGGGAGCTGGTTCAACGCAGAGTCGCTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.13605442176870747	No Hit
GTCCCGGGGCCTTTTTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG	5	0.11337868480725624	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	5	0.11337868480725624	No Hit
GTCCCGGGGCTGTCCCGGGGGCATGATTCTTGCGATTCTGAATCTAAAATTATTTC	5	0.11337868480725624	No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA	5	0.11337868480725624	No Hit
GTCCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	5	0.11337868480725624	No Hit
GTCCCGGGGGAGGCAGAGGAGTCACCAGCCACATCAGCAGTGAAAACTTCAATCTT	5	0.11337868480725624	No Hit
GTCCCGTTACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.11337868480725624	No Hit
GTCCCGGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGCCTCTTAACCCAGAACT	5	0.11337868480725624	No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGACTGTTCAAAGCAGGCC	5	0.11337868480725624	No Hit
GTCCCGGGGTGGATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.11337868480725624	No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGCGTGTTCAAAGCAGGCC	5	0.11337868480725624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.022675736961451247	0.0	0.0	0.0	0.0
10	0.022675736961451247	0.0	0.0	0.0	0.0
11	0.1360544217687075	0.0	0.0	0.0	0.0
12	0.15873015873015872	0.0	0.0	0.0	0.0
13	0.15873015873015872	0.0	0.0	0.0	0.0
14	0.18140589569160998	0.0	0.0	0.0	0.0
15	0.18140589569160998	0.0	0.0	0.0	0.0
16	0.20408163265306123	0.0	0.0	0.0	0.0
17	0.22675736961451248	0.0	0.0	0.0	0.0
18	0.22675736961451248	0.0	0.0	0.0	0.0
19	0.272108843537415	0.0	0.0	0.0	0.0
20	0.3401360544217687	0.0	0.0	0.0	0.0
21	0.3401360544217687	0.0	0.0	0.0	0.0
22	0.36281179138321995	0.0	0.0	0.0	0.0
23	0.40816326530612246	0.0	0.0	0.0	0.0
24	0.40816326530612246	0.0	0.0	0.0	0.0
25	0.40816326530612246	0.0	0.0	0.0	0.0
26	0.40816326530612246	0.0	0.0	0.0	0.0
27	0.40816326530612246	0.0	0.0	0.0	0.0
28	0.47619047619047616	0.0	0.0	0.0	0.0
29	0.4988662131519274	0.0	0.0	0.0	0.0
30	0.4988662131519274	0.0	0.0	0.0	0.0
31	0.4988662131519274	0.0	0.0	0.0	0.0
32	0.4988662131519274	0.0	0.0	0.0	0.0
33	0.4988662131519274	0.0	0.0	0.0	0.0
34	0.4988662131519274	0.0	0.0	0.0	0.0
35	0.5215419501133787	0.0	0.0	0.0	0.0
36	0.5215419501133787	0.0	0.0	0.0	0.0
37	0.5215419501133787	0.0	0.0	0.0	0.0
38	0.5215419501133787	0.0	0.0	0.0	0.0
39	0.5215419501133787	0.0	0.0	0.0	0.0
40	0.5215419501133787	0.0	0.0	0.0	0.0
41	0.5668934240362812	0.0	0.0	0.0	0.0
42	0.5668934240362812	0.0	0.0	0.0	0.0
43	0.5668934240362812	0.0	0.0	0.0	0.0
44	0.5668934240362812	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCAA	15	0.007739279	50.22988	37
AACCAAA	15	0.007739279	50.22988	38
CGGGGGG	45	8.731149E-11	49.659096	5
CGGGGAG	50	3.6379788E-12	49.659096	5
CGGGGAC	25	1.8381548E-5	49.659096	5
GCAGTGG	25	1.8381548E-5	49.659096	12
GGGGGCC	25	1.8381548E-5	49.659096	6
GGAAGCA	25	1.8381548E-5	49.659096	8
GTGGTAT	25	1.8381548E-5	49.659096	15
AAGCAGT	25	1.8381548E-5	49.659096	10
TGGTATC	30	8.67989E-7	49.659092	16
CGGGGTG	15	0.008103372	49.659092	5
TTCAACG	15	0.008103372	49.659092	16
CGGGGGC	35	4.073263E-8	49.659092	5
CGGGGCT	15	0.008103372	49.659092	5
CCCGGGA	35	4.073263E-8	49.659092	3
CGGGGAT	15	0.008103372	49.659092	5
GGGAGCT	15	0.008103372	49.659092	7
GGAAGCC	15	0.008103372	49.659092	8
CCGGGGT	35	4.073263E-8	49.659092	4
>>END_MODULE