FastQCFastQC Report
Fri 17 Jun 2016
SRR1526217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146536
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14100.9622208876999507No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4740.32347000054594094No Hit
TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4340.29617295408636785No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3790.2586395152044549No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3660.24976797510509363No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3260.22247092864552057No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3130.2135993885461593No Hit
TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2940.2006332914778621No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2550.17401867117977834No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1710.1166948736146749No Hit
TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1620.11055303816127095No Hit
TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1490.1016814980619097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC451.2369128E-1050.46782338
TACAATT150.00811202950.4678238
TCACAAT150.00812313550.45036341
TTTATCA150.008234818550.2764637
ATTTATC150.0082798150.2072336
ACTGCGC150.0082798150.2072336
AAAGGCC150.0082798150.2072335
CTCCACG150.00830237450.17268844
CGATTCA252.0489186E-550.12096449
CGATTAA600.050.1209649
CACGATT150.0083476450.10374547
TACCAAG204.129323E-450.1037447
TTCAGTT150.00837034150.06934450
CGGTTGA150.00839308850.03499214
AGTTTCA204.1785778E-449.9835513
CGGCTTG150.00842729649.9835513
CGGCTTA150.00843872149.96642728
GGACTCG150.00845015849.94931410
AATGGTC150.00845015849.94931410
ATTCCCC150.00845015849.94931410
Produced by FastQC (version 0.11.5)