##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526217_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146536 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.423691106622265 38.0 38.0 39.0 33.0 40.0 2 36.111071682044006 38.0 36.0 39.0 30.0 40.0 3 36.807119069716656 38.0 37.0 39.0 31.0 40.0 4 36.46122454550417 38.0 36.0 39.0 31.0 40.0 5 36.32598132882022 38.0 36.0 39.0 29.0 40.0 6 34.50567778566359 39.0 35.0 39.0 18.0 40.0 7 37.10202953540427 39.0 37.0 39.0 33.0 40.0 8 37.05871594693454 39.0 37.0 39.0 33.0 40.0 9 37.02493585194082 39.0 37.0 39.0 32.0 40.0 10 36.446661571217994 38.0 36.0 39.0 31.0 40.0 11 35.757895670688434 38.0 35.0 39.0 29.0 40.0 12 33.722252552273844 38.0 32.0 39.0 21.0 40.0 13 33.40742889119397 38.0 31.0 39.0 20.0 40.0 14 33.50981328820222 38.0 31.0 39.0 20.0 40.0 15 33.55917999672435 38.0 32.0 39.0 20.0 40.0 16 33.74167440082983 38.0 32.0 39.0 22.0 40.0 17 33.73769585630835 38.0 32.0 39.0 22.0 40.0 18 33.16189195829011 37.0 31.0 39.0 19.0 40.0 19 34.04762652181034 38.0 32.0 39.0 24.0 40.0 20 34.082430256046294 38.0 32.0 39.0 24.0 40.0 21 34.862068024239775 38.0 33.0 39.0 26.0 40.0 22 34.575066877763824 38.0 33.0 39.0 25.0 40.0 23 35.10576923076923 38.0 34.0 39.0 27.0 40.0 24 34.670886335098544 38.0 33.0 39.0 25.0 40.0 25 34.9751665119834 38.0 33.0 39.0 27.0 40.0 26 35.54865016105257 38.0 35.0 39.0 29.0 40.0 27 35.464793634328764 38.0 35.0 39.0 28.0 40.0 28 35.35071927717421 38.0 35.0 39.0 28.0 40.0 29 35.18467816782224 38.0 35.0 39.0 27.0 40.0 30 35.024649232952996 38.0 35.0 39.0 27.0 40.0 31 35.28749249331222 38.0 35.0 39.0 28.0 40.0 32 34.945180706447566 38.0 35.0 39.0 27.0 40.0 33 35.14092100234755 38.0 35.0 39.0 28.0 40.0 34 34.92549953595021 38.0 35.0 39.0 27.0 40.0 35 34.88857345635202 38.0 35.0 39.0 27.0 40.0 36 34.253323415406456 37.0 33.0 39.0 27.0 40.0 37 34.05057460282797 37.0 33.0 39.0 27.0 40.0 38 33.70126112354643 36.0 33.0 39.0 25.0 40.0 39 33.21392012884206 36.0 32.0 38.0 23.0 40.0 40 29.922728885734564 35.0 25.0 38.0 9.0 39.0 41 26.677969918654803 32.0 15.0 38.0 5.0 39.0 42 30.71365398263908 35.0 29.0 38.0 10.0 39.0 43 29.258182289676256 34.0 26.0 38.0 5.0 39.0 44 29.208085385161326 34.0 26.0 38.0 4.0 39.0 45 30.282735982966642 35.0 28.0 38.0 4.0 39.0 46 29.738310039853687 35.0 27.0 38.0 4.0 40.0 47 29.2679614565704 35.0 26.0 38.0 4.0 39.0 48 29.333208221870393 35.0 26.0 38.0 4.0 39.0 49 26.833767811322815 33.0 15.0 38.0 4.0 39.0 50 28.910151771578317 34.0 25.0 38.0 4.0 39.0 51 29.108635420647484 35.0 26.0 39.0 4.0 40.0 52 28.857768739422394 35.0 25.0 39.0 4.0 40.0 53 28.454250150133756 35.0 23.0 38.0 4.0 40.0 54 27.502934432494403 34.0 17.0 38.0 4.0 40.0 55 27.813110771414532 34.0 21.0 38.0 4.0 40.0 56 26.534523939509747 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.0961454101229293 1 2 2.4936628348045033 1 3 1.9895111393893075 1 4 1.8705529543519859 1 5 2.067120981816373 1 6 4.751627618652073 1 7 1.4852834528768213 1 8 1.623557463766602 1 9 1.5991738895017704 1 10 1.8038633658697805 1 11 -1.6528850349124866 1 12 -11.856098519492495 1 13 -8.277503766193554 1 14 -4.902570614955891 1 15 -5.582462959164218 1 16 -22.643677627042806 1 17 -4.612782103529295 1 18 -13.376226784887855 1 19 -5.350369161013802 1 20 -5.576457346429557 1 21 -6.099852914128018 1 22 -3.2506346000421757 1 23 -3.3003600823357004 1 24 -4.3890409717827765 1 25 -3.0410701343073647 1 26 1.8668544540671022 1 27 1.6665262891830537 1 28 1.6970367260548684 1 29 1.7698591887357296 1 30 2.1849934468535395 1 31 1.4689418242109298 1 32 2.2506984472556937 1 33 1.6937172576180544 1 34 1.025261162845922 1 35 0.6927875895566658 1 36 1.2653749552284594 1 37 1.32030674387358 1 38 1.4145079567577952 1 39 1.3808980994007811 1 40 4.432299863806797 1 41 -9.179084786782052 1 42 -9.393000432898859 1 43 -16.824653896847437 1 44 -21.985316029358504 1 45 -10.841959159569576 1 46 -17.406575539258398 1 47 -22.946633560131033 1 48 -15.065701160095735 1 49 -19.793439320222483 1 50 -13.882026955365365 1 51 -11.381987430594624 1 52 -11.0038597353525 1 53 -10.569297859384804 1 54 -9.966134495877892 1 55 -10.51848871055359 1 56 -5.832057939677558 2 1 1.859508423631567 2 2 3.169067123173761 2 3 2.775924831103822 2 4 2.4127847925811636 2 5 2.825657499527395 2 6 5.406755076975003 2 7 2.257245395680073 2 8 2.1776613721186564 2 9 2.2938286840983153 2 10 2.2511741200790922 2 11 1.7666317607381146 2 12 -1.277862132152336 2 13 -0.4401899607512618 2 14 3.883288307126712 2 15 3.138321485095304 2 16 2.027676843013502 2 17 2.9089509968007974 2 18 -1.7881987135444817 2 19 1.8854079325480981 2 20 1.8181780312298699 2 21 1.7506089315905484 2 22 1.9346307185342866 2 23 1.4267209972460009 2 24 0.5377294368050585 2 25 1.7098937779280803 2 26 1.5512737489548982 2 27 1.8885173573919758 2 28 1.628078860808408 2 29 2.161998103051559 2 30 2.2117610815932167 2 31 1.3218446563098993 2 32 2.0537552279275175 2 33 0.8828382251362896 2 34 1.2702751707791293 2 35 0.8646274830408984 2 36 1.2981341019927655 2 37 1.3643277141656611 2 38 1.0554333125038227 2 39 2.6236652903247517 2 40 4.715426545452246 2 41 -6.335723992351941 2 42 -6.9641357689505625 2 43 -13.993165172540206 2 44 -14.349106215483715 2 45 -6.136957911337902 2 46 -10.791807571656943 2 47 -17.885914023918446 2 48 -9.288524382752527 2 49 -13.04605367211286 2 50 -7.770740167204149 2 51 -3.338743137787212 2 52 -3.2737551612768847 2 53 -3.2046477803301343 2 54 -2.0869910601895647 2 55 -2.5957126433155118 2 56 0.7475931102239706 3 1 1.9214973711447598 3 2 3.0295857398625117 3 3 2.663849614180016 3 4 2.6089039193846943 3 5 2.8688922273411563 3 6 5.507152666432631 3 7 2.205033648036853 3 8 2.164433395823991 3 9 2.3370134006595435 3 10 2.3395440033028194 3 11 3.284798348220299 3 12 5.019429758523067 3 13 3.7805597079241906 3 14 4.6703653994724945 3 15 4.660989085305346 3 16 4.258903869094368 3 17 4.947959773775615 3 18 5.092749499803524 3 19 3.9870558033190235 3 20 4.205690221472679 3 21 3.059478427227063 3 22 3.6050815699758587 3 23 3.4282463404482257 3 24 3.4770657874839586 3 25 3.247739793281532 3 26 2.6897299015892386 3 27 2.5414892760736834 3 28 2.3326123808504207 3 29 2.8075183451060184 3 30 2.5345587110598444 3 31 2.4409464542144264 3 32 2.6222831467092433 3 33 2.2662494926714842 3 34 2.012367141472538 3 35 1.6206725931906831 3 36 2.773140978539992 3 37 2.7796461608161565 3 38 2.723883713844124 3 39 3.3191467736585025 3 40 6.837479007256157 3 41 7.712836933856458 3 42 4.038289776797232 3 43 -2.06038029594297 3 44 2.2035181249928932 3 45 4.408464808774124 3 46 2.886945147704914 3 47 -2.193208165100213 3 48 2.8456558077903438 3 49 4.73812276758607 3 50 3.5706366425780516 3 51 4.2634673595746975 3 52 4.050542805765701 3 53 3.906677267805698 3 54 4.843093208770952 3 55 4.665796196173375 3 56 5.115100799454044 4 1 1.968128008669268 4 2 2.9677463405607796 4 3 2.7898719012334396 4 4 2.5886016572009467 4 5 2.7075263083589007 4 6 5.698362193430576 4 7 2.189777059964733 4 8 2.2818947760908657 4 9 2.335817743287116 4 10 2.1827694086306693 4 11 2.930620721361052 4 12 5.503814473239217 4 13 5.439510399014154 4 14 4.925351290715497 4 15 4.677895680551416 4 16 4.7650973742835205 4 17 5.097679989950464 4 18 5.047960174632543 4 19 4.144882576478906 4 20 3.78175794350625 4 21 2.7265835015969557 4 22 3.163525302339913 4 23 3.0527620992123943 4 24 3.5305834114736285 4 25 2.919962281205393 4 26 2.2243800522420685 4 27 2.2347792468996417 4 28 2.370945156210311 4 29 2.5603520530786597 4 30 3.0262847620426783 4 31 1.2527499837949918 4 32 2.2970404282626404 4 33 1.8403563366142848 4 34 1.5735130594982678 4 35 1.7613058133351203 4 36 2.291530188927858 4 37 2.7379416316660325 4 38 2.635739852349076 4 39 3.2026658324370203 4 40 6.058460402827436 4 41 9.308872134009498 4 42 5.037022379982464 4 43 6.587092084371729 4 44 5.5454283072110755 4 45 4.698387808439339 4 46 5.2843099188560885 4 47 4.448692451858992 4 48 4.992339054239245 4 49 7.045119754529495 4 50 5.680469728808863 4 51 4.325689369235604 4 52 4.212889163793346 4 53 4.60068463305511 4 54 6.12034224028848 4 55 4.732585616996943 4 56 5.089370252799494 5 1 2.0681914325849604 5 2 3.3511195171232018 5 3 2.83831801529891 5 4 2.6218504253133545 5 5 2.982737314847654 5 6 5.754126451614702 5 7 2.3048182855099313 5 8 2.21671450580741 5 9 2.3118630182054005 5 10 2.3314740971216636 5 11 3.055053565191855 5 12 5.612171793822718 5 13 5.015106827759823 5 14 5.703684705361539 5 15 5.1267662469757695 5 16 5.002449181865117 5 17 4.892064534030091 5 18 5.015540796186919 5 19 4.271730407868866 5 20 3.8477676034564894 5 21 2.8623350749979366 5 22 3.380361912575559 5 23 2.9816785260065544 5 24 3.0413898709555056 5 25 2.9765315352425716 5 26 1.7876332112409443 5 27 2.1574508573792244 5 28 2.2127024865513363 5 29 1.88400917363289 5 30 2.316497963974669 5 31 1.4913214820630287 5 32 1.7981625436941684 5 33 1.2745174333601526 5 34 1.2227788057050262 5 35 1.4449912307132706 5 36 2.796335770232446 5 37 2.9042098660417963 5 38 2.8237673685709623 5 39 3.0812578215085935 5 40 6.535822943687073 5 41 9.270403089759121 5 42 5.676554994511417 5 43 6.72267290107661 5 44 6.721551642333289 5 45 5.17245718444558 5 46 5.0319559158312614 5 47 4.354654299934321 5 48 5.036833370480643 5 49 7.599152051938873 5 50 5.3738150964236375 5 51 4.75380080017672 5 52 4.73098937358014 5 53 4.55682455597066 5 54 5.2962704249008645 5 55 4.081148821368313 5 56 3.8926585388901422 6 1 1.790134481161175 6 2 3.056007593870575 6 3 2.665864840333171 6 4 2.4370374716561756 6 5 2.8389375077312593 6 6 5.39949978291768 6 7 1.8846873276905285 6 8 2.0028421135430534 6 9 1.9922853317935392 6 10 1.9581935529868488 6 11 3.1666215059055247 6 12 4.97243269428462 6 13 4.826486960747992 6 14 5.396568315330576 6 15 5.417868221494828 6 16 4.663989205214186 6 17 4.450970173337737 6 18 5.269741090588219 6 19 4.208332610802721 6 20 3.9189061243437493 6 21 2.9633197965856795 6 22 3.2040764448328005 6 23 2.8268132907393095 6 24 3.965073947905914 6 25 3.391865782235108 6 26 2.255908933285511 6 27 2.435279394001732 6 28 2.500149824249931 6 29 2.360685484480058 6 30 2.6431818886485416 6 31 2.194889828847458 6 32 2.379858904285001 6 33 2.2791618676528245 6 34 1.7312555327747177 6 35 1.9845577501792917 6 36 2.2025334251738187 6 37 2.188662431901399 6 38 2.489520376032509 6 39 2.6490716380661254 6 40 6.092342917292477 6 41 2.615334819112963 6 42 4.4411608520820955 6 43 5.0307029503126905 6 44 4.837916293873818 6 45 3.801359270634098 6 46 4.157507918267683 6 47 4.606189754987366 6 48 3.9482084275843476 6 49 6.182218901337379 6 50 4.132710583962339 6 51 4.581499901745168 6 52 5.077113935785103 6 53 4.445688065437185 6 54 4.3556075143887085 6 55 4.474225314947319 6 56 4.570492151397122 7 1 2.0042968300385553 7 2 3.2858709947184295 7 3 2.9166891455616266 7 4 2.743312658760381 7 5 2.7607986057581613 7 6 5.804403744816462 7 7 2.345670213543883 7 8 2.2888367751853878 7 9 2.323996223089928 7 10 2.16330162856125 7 11 3.291026174351643 7 12 5.223518683712584 7 13 5.253032205946091 7 14 5.517911278642451 7 15 5.545570110854747 7 16 4.608198133879078 7 17 5.301286811219143 7 18 5.212379914056058 7 19 4.466981273596467 7 20 3.8866927993843134 7 21 3.188603723942144 7 22 3.466231675899948 7 23 3.15276712964652 7 24 3.8617871943600903 7 25 3.6108169519634856 7 26 3.1104910236188985 7 27 2.7328928341022163 7 28 2.811082990105376 7 29 2.8855193150133687 7 30 3.197847717925768 7 31 2.3350478861039576 7 32 2.663733923911316 7 33 2.323831360508848 7 34 2.399309125698778 7 35 1.890613122555905 7 36 2.5401845477990292 7 37 2.945296126358926 7 38 2.698972409318742 7 39 3.524588464360157 7 40 6.77491998328923 7 41 0.8184802631910486 7 42 5.243144418314369 7 43 6.525469266322528 7 44 6.0336822435257815 7 45 5.266424429999777 7 46 5.903229381605726 7 47 5.985867360053572 7 48 5.698561701253681 7 49 7.670050586060256 7 50 5.5373790300815635 7 51 5.335699933147275 7 52 5.032522948188937 7 53 5.39604657279051 7 54 6.112293545686136 7 55 5.252003092725097 7 56 4.702328651109269 8 1 1.7458082400264274 8 2 2.894982448802878 8 3 2.6968126159617967 8 4 2.5658790792211903 8 5 2.625311889850323 8 6 5.581908981284329 8 7 1.9747904869426236 8 8 1.8294056209576297 8 9 2.1786704596680337 8 10 2.044213325176834 8 11 2.7092821549491504 8 12 5.014556055556916 8 13 4.7767341131228065 8 14 4.890389505960279 8 15 5.249213590074241 8 16 4.618588388536779 8 17 4.579811780902752 8 18 5.189201653665805 8 19 3.771302737602639 8 20 3.650638340118519 8 21 3.0216155197750183 8 22 3.619990908619606 8 23 2.5013780876445324 8 24 3.385985187966085 8 25 2.8946059498688967 8 26 2.2764870549274647 8 27 1.8442091167612418 8 28 2.2662147286127095 8 29 2.347844306745259 8 30 2.3192510913134896 8 31 1.4507462655549617 8 32 1.24462357224116 8 33 1.4506641242614648 8 34 1.2092804629273104 8 35 1.2118842062291506 8 36 2.071327751414948 8 37 1.8804741662663247 8 38 2.185522955013809 8 39 2.505185972903867 8 40 4.7438043400304934 8 41 -1.8345899605990503 8 42 3.204807961473886 8 43 5.041233790630766 8 44 4.516457020286886 8 45 3.5434611403955465 8 46 4.5130849653340945 8 47 4.912336327091385 8 48 4.419678446926703 8 49 6.832766934864132 8 50 4.202934095675214 8 51 4.528953157709061 8 52 4.01747499316744 8 53 4.141636915641353 8 54 4.84539904673343 8 55 4.406258411874035 8 56 4.016709645486955 9 1 1.53919815157316 9 2 2.7832803211433017 9 3 2.3997769955936405 9 4 1.939534350557551 9 5 2.623638449735573 9 6 5.335375392950596 9 7 1.8952423157736078 9 8 1.8555830056097875 9 9 2.110716837865496 9 10 1.999448802107267 9 11 2.897185573548242 9 12 5.083884288882281 9 13 4.923638248910514 9 14 4.995875998907927 9 15 5.17958652815696 9 16 4.079322311558528 9 17 4.857961434261583 9 18 4.818713965403788 9 19 3.7289886089868105 9 20 3.8803179958679834 9 21 2.6314051444463047 9 22 2.818592390809421 9 23 2.6506130864492192 9 24 3.404751623538644 9 25 2.9482157992592875 9 26 1.9510627183269378 9 27 2.1024567858982266 9 28 1.9441970379600662 9 29 1.8504142326357709 9 30 2.0375752605700015 9 31 1.6398449985217312 9 32 1.7627325849114897 9 33 1.6695261849834395 9 34 1.5443868459060326 9 35 1.1445282416104519 9 36 2.633245035660707 9 37 2.1781074438183197 9 38 2.588941554105375 9 39 3.0642345026671904 9 40 5.620149068694133 9 41 2.6480779810696085 9 42 4.846392948325427 9 43 6.002386073681205 9 44 5.6195528844991784 9 45 4.616136253950412 9 46 5.616479658138303 9 47 5.854004985948659 9 48 5.028392288667085 9 49 8.084500140697266 9 50 5.68554227162549 9 51 5.24016520647789 9 52 5.619674371603967 9 53 4.859602490587562 9 54 6.013818841139361 9 55 5.07222781182622 9 56 4.484974049025087 10 1 1.48759824927712 10 2 2.973497712447653 10 3 2.4133944846019446 10 4 2.0028587276948215 10 5 2.4387947760667927 10 6 5.17672859275519 10 7 1.6864001076641841 10 8 1.7591003480621623 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1.817729503605186 119 37 1.7737782964290219 119 38 2.0193777735369665 119 39 1.9702986137048626 119 40 6.065456405111846 119 41 1.3783324424047052 119 42 -3.0609930397776743 119 43 -11.458453318255344 119 44 -10.186296421515365 119 45 -6.423685292645757 119 46 -16.503811154816095 119 47 -15.500836388803485 119 48 -13.29778087722849 119 49 -12.178966481906828 119 50 -11.490432612710269 119 51 -8.6159154280231 119 52 -8.066379825355714 119 53 -9.188150318401199 119 54 -10.93776741133916 119 55 -7.968585142569019 119 56 -5.73751438455059 120 1 0.1550467618629341 120 2 1.6148592685117222 120 3 0.7945729743044652 120 4 0.6779731441707995 120 5 1.0550991238928802 120 6 4.075470011519492 120 7 0.38548477585639773 120 8 0.6055534373070088 120 9 0.5250870330162982 120 10 0.556811309499345 120 11 -2.9722408013778363 120 12 -10.077381234476675 120 13 -10.964475293372157 120 14 -13.634638489560437 120 15 -12.985108918324414 120 16 -18.382475441250456 120 17 -16.771252048884485 120 18 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-8.539449369070713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1128.0 5 51.0 6 140.0 7 175.0 8 154.0 9 126.0 10 138.0 11 125.0 12 141.0 13 187.0 14 225.0 15 344.0 16 516.0 17 640.0 18 792.0 19 988.0 20 1136.0 21 1545.0 22 1798.0 23 2243.0 24 2645.0 25 2971.0 26 3312.0 27 3631.0 28 4092.0 29 4817.0 30 5643.0 31 6463.0 32 7588.0 33 8738.0 34 10169.0 35 12012.0 36 15105.0 37 18754.0 38 17722.0 39 10275.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.32508052628705 0.3671452748812578 0.2845717093410493 0.02320248949063711 8 99.33326964022493 0.5909810558497571 0.03958071736638096 0.036168586558934325 9 97.37607140907355 1.5354588633509854 0.5234208658623136 0.5650488617131627 10 49.56870666593874 33.15772233444341 10.115602991756292 7.15796800786155 11 35.84102200141945 31.75533657258285 17.21556477589125 15.188076650106458 12 37.87055740568871 14.81274226128733 20.23803024512748 27.07867008789649 13 25.66804769672853 16.28568893378564 24.989591083141647 33.05667228634419 14 25.271785492489645 18.51212371443585 30.472050282875294 25.744040510199206 15 29.17222461641596 17.841531736748138 27.503813608597206 25.482430038238697 16 33.545699750759674 19.063812352760422 25.145276383625255 22.245211512854652 17 30.4157340175793 19.874979527215157 26.76407162744991 22.945214827755635 18 29.5410652745078 22.31685262906473 26.029958712935475 22.112123383491998 19 30.718204113978096 25.63755729988591 21.555687632788857 22.088550953347134 20 28.779858061078816 26.58315175442791 21.763512544313222 22.873477640180052 21 27.36187694491456 22.670879510836926 28.39438772724791 21.5728558170006 22 26.119861331003985 21.712753180105913 26.32936616258121 25.838019326308892 23 28.14461975214282 22.707048097395862 25.39171261669487 23.756619533766447 24 28.118687558006222 21.91884588087569 27.995168422776658 21.96729813834143 25 29.97584079493339 21.21096309237825 24.81300502293077 24.00019108975759 26 24.296137544108714 18.874093083890166 30.109956113109416 26.7198132588917 27 24.41232127120323 18.559102615776638 29.892540698587933 27.136035414432204 28 23.200535654502847 24.30259013548506 29.673483052410788 22.823391157601307 29 27.789574301027546 21.894650599119245 30.050182637490185 20.26559246236302 30 24.172508652054308 18.10275978347679 37.871082684287046 19.853648880181847 31 25.093528126706715 19.972692517749863 35.69224467504096 19.241534680502458 32 30.46772001010991 16.49350028348737 35.26036436666188 17.77841533974083 33 26.277906134222867 18.163424337293396 36.4273312644401 19.131338264043638 34 21.35526558371059 20.430724269550907 41.36878546408014 16.845224682658362 35 21.536654648749042 20.939172948759968 43.356003496121495 14.168168906369496 36 21.38980111560966 25.194071019410515 37.96623130124874 15.44989656373108 37 22.901979554856577 21.552873602516325 39.90358644739992 15.641560395227186 38 24.68879810028263 21.732465594996135 38.271503555126706 15.307232749594531 39 23.00801444240816 20.74318225334391 36.774802374244366 19.47400093000356 40 16.734259004674183 14.643249931262028 35.54028045092109 33.0822106131427 41 14.811525353712693 14.923749526661847 33.707872904402905 36.556852215222555 42 20.087204931589234 19.287598583944998 38.43441177676631 22.190784707699457 43 15.919040192618128 16.485403157405127 40.696735888806806 26.898820761169933 44 16.678326954470005 15.736587239529747 38.056435792968166 29.528650013032088 45 20.30747767093871 19.157171971385974 41.09641504014444 19.438935317530877 46 19.37409402948336 20.09476793479747 38.509558843639745 22.021579192079425 47 17.644850115033552 21.306808484492656 40.53072454071915 20.51761685975464 48 19.1547100681509 21.47949662663285 42.036187650810355 17.32960565440588 49 17.316136114160262 17.252332601536775 34.84426454445664 30.587266739846324 50 21.08467904516535 19.14589725891655 40.51145022517751 19.25797347074059 51 19.70566089396947 21.856389896639058 39.96029844616332 18.477650763228148 52 17.859290438965726 20.13885092658394 41.88829060296288 20.113568031487457 53 18.69864231121563 19.21820095981624 43.195148962933594 18.888007766034537 54 16.424598586586942 20.705885572377557 39.7465981624262 23.1229176786093 55 18.156877501453835 21.927273971196932 39.11538329969555 20.800465227653678 56 21.810845927648227 20.460917732339464 36.874102441359504 20.854133898652808 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 2.5 5 2.0 6 21.0 7 40.0 8 111.5 9 183.0 10 335.0 11 487.0 12 569.5 13 812.5 14 973.0 15 1106.5 16 1240.0 17 1403.5 18 1567.0 19 1892.5 20 2218.0 21 2688.0 22 3782.0 23 4406.0 24 4919.0 25 5432.0 26 5470.5 27 5509.0 28 5532.0 29 5077.0 30 4599.0 31 3956.5 32 3314.0 33 2958.5 34 2603.0 35 2503.5 36 2404.0 37 2480.0 38 2538.5 39 2521.0 40 2703.5 41 2886.0 42 3843.5 43 4801.0 44 5007.5 45 5214.0 46 4980.5 47 4838.0 48 4929.0 49 5161.5 50 5394.0 51 5679.5 52 5965.0 53 6499.5 54 7516.5 55 7999.0 56 9135.0 57 10271.0 58 10185.5 59 10100.0 60 8904.0 61 7708.0 62 6743.5 63 5054.0 64 4329.0 65 3801.5 66 3274.0 67 2890.0 68 2506.0 69 2130.0 70 1754.0 71 1435.0 72 888.0 73 660.0 74 506.0 75 352.0 76 266.5 77 181.0 78 121.0 79 47.0 80 33.0 81 21.5 82 10.0 83 7.5 84 5.0 85 3.0 86 1.0 87 2.0 88 2.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.018425506360211827 14 0.003412130807446634 15 0.23816673035977506 16 0.06210078069552875 17 0.0 18 6.824261614893268E-4 19 0.10714090735382431 20 0.09212753180105912 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005459409291914614 26 0.01569580171425452 27 0.10577605503084567 28 0.11805972593765354 29 0.04845225746574221 30 0.026614620298083747 31 0.038215865043402304 32 0.0989517934159524 33 0.16514713108041712 34 0.05800622372659278 35 0.06005350221106076 36 0.04708740514276355 37 0.19858601299339412 38 0.2791123000491347 39 0.20472784844679806 40 0.7206420265327291 41 0.881012174482721 42 0.14603919855871594 43 0.23202489490637113 44 0.5063602118250805 45 0.21428181470764862 46 0.19380902986296883 47 0.03889829120489163 48 0.16582955724190643 49 0.5295627013157177 50 0.14126221542829068 51 0.3043620680242398 52 0.13102582300595075 53 0.17606594966424632 54 0.2490855489436043 55 0.25318010591254025 56 0.20882240541573402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 146536.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.03630848417926 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6309554906342 59.972760145370806 2 8.235995719083782 11.536374737122628 3 2.7170499687983107 5.7087644934506425 4 1.167999981061855 3.2720962793265445 5 0.652999669337156 2.2866843140882054 6 0.37199980677420524 1.5632095933975985 7 0.2739998575826107 1.3432956985193834 8 0.16799991267683642 0.9412874967640074 9 0.1489999225526338 0.9391864086013569 >10 0.5789996990468078 6.858652124856842 >50 0.03299998284722739 1.6577585601486926 >100 0.018999990124161224 2.9324187120162972 >500 0.0 0.0 >1k 9.999994802190118E-4 0.9875114363369905 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1410 0.9622208876999507 No Hit TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 474 0.32347000054594094 No Hit TATGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 434 0.29617295408636785 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 379 0.2586395152044549 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 366 0.24976797510509363 No Hit TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 326 0.22247092864552057 No Hit TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 313 0.2135993885461593 No Hit TATGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 294 0.2006332914778621 No Hit TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 255 0.17401867117977834 No Hit TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 171 0.1166948736146749 No Hit TATGTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 162 0.11055303816127095 No Hit TATGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 149 0.1016814980619097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.013648523229786536 0.0 0.0 0.0 0.0 7 0.013648523229786536 0.0 0.0 0.0 0.0 8 0.013648523229786536 0.0 0.0 0.0 0.0 9 0.014330949391275863 0.0 0.0 0.0 0.0 10 0.019790358683190477 0.0 0.0 0.0 0.0 11 0.02388491565212644 0.0 0.0 0.0 0.0 12 0.027297046459573072 0.0 0.0 0.0 0.0 13 0.028661898782551726 0.0 0.0 0.0 0.0 14 0.03139160342850904 0.0 0.0 0.0 0.0 15 0.034121308074466346 0.0 0.0 0.0 0.0 16 0.036168586558934325 0.0 0.0 0.0 0.0 17 0.037533438881912976 0.0 0.0 0.0 0.0 18 0.04299284817382759 0.0 0.0 0.0 0.0 19 0.049134683627231536 0.0 0.0 0.0 0.0 20 0.053229240596167494 0.0 0.0 0.0 0.0 21 0.05937107604957144 0.0 0.0 0.0 0.0 22 0.06141835453403942 0.0 0.0 0.0 0.0 23 0.06483048534148605 0.0 0.0 0.0 0.0 24 0.06756018998744336 0.0 0.0 0.0 0.0 25 0.0737020254408473 0.0 0.0 0.0 0.0 26 0.07711415624829393 0.0 0.0 0.0 0.0 27 0.08257356554020855 0.0 0.0 0.0 0.0 28 0.0866681225091445 0.0 0.0 0.0 0.0 29 0.0914451056395698 0.0 0.0 0.0 0.0 30 0.0941748102855271 0.0 0.0 0.0 0.0 31 0.09622208876999509 0.0 0.0 0.0 0.0 32 0.09826936725446307 0.0 0.0 0.0 0.0 33 0.09963421957744172 0.0 0.0 0.0 0.0 34 0.1016814980619097 0.0 0.0 0.0 0.0 35 0.10509362886935633 0.0 0.0 0.0 0.0 36 0.10850575967680297 0.0 0.0 0.0 0.0 37 0.1119178904842496 0.0 0.0 0.0 0.0 38 0.1146475951302069 0.0 0.0 0.0 0.0 39 0.11533002129169624 0.0 0.0 0.0 0.0 40 0.1166948736146749 0.0 0.0 0.0 0.0 41 0.11874215209914288 0.0 0.0 0.0 0.0 42 0.12078943058361086 0.0 0.0 0.0 0.0 43 0.12488398755254682 0.0 0.0 0.0 0.0 44 0.1296609706829721 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 45 1.2369128E-10 50.467823 38 TACAATT 15 0.008112029 50.46782 38 TCACAAT 15 0.008123135 50.450363 41 TTTATCA 15 0.0082348185 50.27646 37 ATTTATC 15 0.00827981 50.20723 36 ACTGCGC 15 0.00827981 50.20723 36 AAAGGCC 15 0.00827981 50.20723 35 CTCCACG 15 0.008302374 50.172688 44 CGATTCA 25 2.0489186E-5 50.120964 49 CGATTAA 60 0.0 50.12096 49 CACGATT 15 0.00834764 50.103745 47 TACCAAG 20 4.129323E-4 50.10374 47 TTCAGTT 15 0.008370341 50.069344 50 CGGTTGA 15 0.008393088 50.034992 14 AGTTTCA 20 4.1785778E-4 49.98355 13 CGGCTTG 15 0.008427296 49.98355 13 CGGCTTA 15 0.008438721 49.966427 28 GGACTCG 15 0.008450158 49.949314 10 AATGGTC 15 0.008450158 49.949314 10 ATTCCCC 15 0.008450158 49.949314 10 >>END_MODULE