FastQCFastQC Report
Fri 17 Jun 2016
SRR1526216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30458
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7052.314662814367326No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2170.7124564974719285No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1950.640225884825005No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1860.6106769978330816No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC550.18057653161730908No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG530.17401011228577057No Hit
GGTACAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC520.17072690262000131No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC490.16087727362269355No Hit
GGTACAGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTT450.14774443495961653No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.13789480596230877No Hit
GGTACAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG410.1346115962965395No Hit
GGTACAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG370.12147875763346247No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG360.11819554796769323No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG360.11819554796769323No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC360.11819554796769323No Hit
GGTACAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC350.11491233830192395No Hit
GGTACAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA340.1116291286361547No Hit
GGTACAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG320.10506270930461618No Hit
GGTACAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC310.10177949963884694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAG150.008207723550.2214933
TGCTCGT150.008207723550.2214937
ACGGGTA150.008207723550.2214933
TCGTCTC150.008207723550.2214940
GAATTGT150.008207723550.2214933
CGTCTCA309.836731E-750.2214941
CTTTTTA150.008207723550.2214933
ACGATTC252.0061623E-550.1386248
CGATTCA252.0061623E-550.1386249
GCAAGCA150.00826160850.13861545
GTGTATG150.00826160850.13861545
CAACTCA150.00826160850.13861546
GTTCTGA150.00826160850.13861546
TGTTACT150.00826160850.13861548
AACTCAT150.00826160850.13861547
ATGTTAC150.00826160850.13861547
ACGATTA150.00826160850.13861548
TGTATGT150.00826160850.13861546
GTTACTT150.00826160850.13861549
CAAGCAC150.00826160850.13861546
Produced by FastQC (version 0.11.5)