FastQCFastQC Report
Fri 17 Jun 2016
SRR1526215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11081
Sequences flagged as poor quality0
Sequence length56
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC720.6497608519086725No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG550.49634509520801373No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC380.34292933850735496No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC300.2707336882952802No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC250.2256114069127335No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC240.21658695063622416No Hit
CATCCCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC220.19853803808320547No Hit
CATCCCGGGGAGACTTGCTTCAGCATAGAGATAGAGGGAGCAGACGAAGCTACTCT180.16244021297716812No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG170.1534157567006588No Hit
CATCCCGGGGAGACTTGCTTCAGCATAGAGATAGAGGGAGAAGACGAAGCTACTCT170.1534157567006588No Hit
CATCCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG170.1534157567006588No Hit
CATCCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1534157567006588No Hit
CATCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC150.1353668441476401No Hit
CATCCCGGGAAGCTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1353668441476401No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC120.10829347531811208No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTACCAAGCGC120.10829347531811208No Hit
CATCCCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC120.10829347531811208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT203.8487703E-450.39908236
CGCTTGC203.8487703E-450.39908239
CTTGCCT251.8635643E-550.39908241
TAGAGCG203.8487703E-450.39908234
TGGTCTG150.00810237850.16895350
GCAAGCG150.00810237850.16895349
TAGCAAG150.00810237850.16895347
CTGGTCT150.00810237850.16895349
CTAGCAA150.00810237850.16895346
AGCAAGC150.00810237850.16895348
GCCTGGT150.00810237850.16895347
CCTAGCA150.00810237850.16895345
CCTGGTC150.00810237850.16895348
GTAGAGC203.937502E-450.1689533
AGTAGCA203.937502E-450.1689528
CAGTAGC203.937502E-450.1689527
AGTGGTA203.937502E-450.1689529
TAGCATA203.937502E-450.1689530
AGCATAT203.937502E-450.1689531
GTGGTAG203.937502E-450.1689530