FastQCFastQC Report
Fri 17 Jun 2016
SRR1526214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20173
Sequences flagged as poor quality0
Sequence length56
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC550.27264164972983695No Hit
ACCTGAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG350.17349923164625985No Hit
ACCTGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG330.16358498983790215No Hit
ACCTGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA330.16358498983790215No Hit
ACCTGAGGGATGATACGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1487136271253656No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11897090170029247No Hit
ACCTGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC230.11401378079611361No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT230.11401378079611361No Hit
ACCTGAGGGTGTTGTTGTTTTTGAGACATGATCTGGTGAGATCACAGGTCTGGCTA210.10409953898775591No Hit
ACCTGAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.10409953898775591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCCT203.9429928E-450.359341
CTTTTGA150.008240906550.10750230
TAGCGCC204.042233E-450.10750244
TTTTGCT204.042233E-450.10750229
TTTTGAT150.008240906550.10750231
ACAAAAC309.820378E-750.10750248
TGGTAGA309.820378E-750.10750231
CCAAAAC204.042233E-450.10750243
CATCTTT150.008240906550.10750227
TGTTTTG204.042233E-450.10750227
GATGTGG150.008240906550.10750214
GTTTTGC204.042233E-450.10750228
ATGTGGA150.008240906550.10750215
GCCGGAA204.042233E-450.10750248
GCGCCGG204.042233E-450.10750246
TGTAGCG204.042233E-450.10750242
CTTAATC150.008240906550.10750215
GTGGTAG309.820378E-750.10750230
AGCGCCG204.042233E-450.10750245
CTATGGC150.008240906550.10750232