FastQCFastQC Report
Fri 17 Jun 2016
SRR1526213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45493
Sequences flagged as poor quality0
Sequence length56
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2620.5759127777899896No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG910.20003077396522542No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC860.18904007209900425No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC810.17804937023278306No Hit
TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG800.17585122985953883No Hit
TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG740.1626623876200734No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG640.14068098388763106No Hit
TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG610.13408656276789835No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC600.13188842239465412No Hit
TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG540.11869958015518871No Hit
TGCGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG480.1055107379157233No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.10111445716923483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA150.00807413650.4704639
CCACTGA150.00807413650.4704640
TCGTCTC150.00807413650.4704640
AAGAGCC354.8574293E-850.3581835
TAAGACC150.00814564750.35817335
ATGCTCG150.00814564750.35817336
CAGCGTA150.00814564750.35817336
AGCGTAG150.00814564750.35817337
GTACGCC150.00814564750.35817337
ATATGCC251.9833864E-550.30222334
GTAAGAC150.008181578550.3022234
TTGTTGG150.00825379650.1906949
AACGATT309.988726E-750.1906947
CCGCTCT150.00825379650.1906948
ACCGCTC150.00825379650.1906947
GACCGCT150.00825379650.1906946
TCTGAGT800.050.1906948
ACGATTC150.00825379650.1906948
CAACGAT309.988726E-750.1906946
CGATTCA150.00825379650.1906949