##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526213_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45493 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.65849691161277 39.0 38.0 39.0 34.0 40.0 2 37.198931703778605 39.0 38.0 39.0 33.0 40.0 3 37.2762403007056 39.0 38.0 39.0 33.0 40.0 4 36.96179632031301 39.0 37.0 39.0 33.0 40.0 5 36.98193128613193 39.0 37.0 39.0 33.0 40.0 6 34.885652737783836 39.0 35.0 39.0 22.0 40.0 7 37.21352735585695 39.0 38.0 39.0 33.0 40.0 8 37.15316642120766 39.0 38.0 39.0 33.0 40.0 9 36.371771481326796 39.0 35.0 39.0 30.0 40.0 10 35.89319235926406 39.0 35.0 39.0 29.0 40.0 11 35.19512892093289 38.0 33.0 39.0 28.0 40.0 12 33.85309827885609 38.0 32.0 39.0 21.0 40.0 13 33.62301892598861 38.0 32.0 39.0 20.0 40.0 14 33.74277361352296 38.0 32.0 39.0 21.0 40.0 15 33.66638823555272 38.0 32.0 39.0 20.0 40.0 16 33.734530587123295 38.0 32.0 39.0 22.0 40.0 17 33.70045941133801 38.0 32.0 39.0 22.0 40.0 18 33.124238893895765 37.0 31.0 39.0 19.0 40.0 19 34.00749565867276 37.0 32.0 39.0 25.0 40.0 20 34.07119776668938 37.0 32.0 39.0 25.0 40.0 21 34.71070274547733 38.0 33.0 39.0 26.0 40.0 22 34.29830963005298 38.0 33.0 39.0 24.0 40.0 23 34.81691688831249 38.0 33.0 39.0 26.0 40.0 24 34.4168993031895 38.0 33.0 39.0 25.0 40.0 25 34.671421977007455 38.0 33.0 39.0 26.0 40.0 26 35.064559382762184 38.0 35.0 39.0 27.0 40.0 27 34.9375288505924 38.0 35.0 39.0 27.0 40.0 28 34.897896379662804 38.0 34.0 39.0 27.0 40.0 29 34.682038995010224 38.0 34.0 39.0 26.0 40.0 30 34.52836700151672 38.0 33.0 39.0 26.0 40.0 31 34.831402633372164 38.0 35.0 39.0 27.0 40.0 32 34.5221902270679 38.0 34.0 39.0 26.0 40.0 33 34.58956322950784 38.0 34.0 39.0 27.0 40.0 34 34.40867825819357 38.0 33.0 39.0 26.0 40.0 35 34.38792781307014 38.0 33.0 39.0 26.0 40.0 36 33.99070186622118 37.0 33.0 38.0 26.0 39.0 37 33.74712593146198 37.0 33.0 38.0 25.0 39.0 38 33.32565449629613 36.0 32.0 38.0 24.0 39.0 39 32.89015892554899 36.0 32.0 38.0 21.0 39.0 40 29.781856549359244 35.0 25.0 38.0 9.0 39.0 41 26.699887894840966 32.0 16.0 38.0 5.0 39.0 42 30.576879959554216 35.0 28.0 38.0 10.0 39.0 43 29.17982986393511 33.0 26.0 38.0 5.0 39.0 44 29.168685292242763 34.0 26.0 38.0 4.0 39.0 45 30.179412217264193 34.0 28.0 38.0 4.0 39.0 46 29.738531202602598 35.0 27.0 38.0 4.0 39.0 47 29.258457345086057 35.0 26.0 38.0 4.0 39.0 48 29.29160530191458 34.0 26.0 38.0 4.0 39.0 49 26.861319323852022 33.0 16.0 38.0 4.0 39.0 50 28.86646297232541 34.0 25.0 38.0 4.0 39.0 51 29.087639856681246 35.0 26.0 38.0 4.0 40.0 52 28.825555579979337 35.0 25.0 38.0 4.0 40.0 53 28.429362759105796 35.0 23.0 38.0 4.0 40.0 54 27.333941485503264 33.0 17.0 38.0 4.0 39.0 55 27.673730024399358 34.0 20.0 38.0 4.0 39.0 56 26.494054030290375 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9784966725039368 1 2 1.3596736027696537 1 3 1.3969642135120353 1 4 1.889904957673302 1 5 1.7850069001081295 1 6 4.039294421579392 1 7 1.6512844599581769 1 8 1.6415267900614268 1 9 1.4441868208205477 1 10 1.3626148358787304 1 11 -3.5501362845949913 1 12 -12.820665419984373 1 13 -7.538875275772458 1 14 -6.811910115094271 1 15 -8.755653402115492 1 16 -23.051138020959748 1 17 -4.848731082696791 1 18 -13.32576413629106 1 19 -6.554879652989992 1 20 -6.4577193745241495 1 21 -5.877275614707479 1 22 -2.1679566592485138 1 23 -3.494133285673982 1 24 -2.4747772079554693 1 25 -2.7804621683419164 1 26 2.2364970982449606 1 27 2.799248286349851 1 28 1.3782838642362307 1 29 2.2724201456344346 1 30 1.9467938523636548 1 31 1.312258334757587 1 32 1.7593722629954485 1 33 1.8239711936984975 1 34 0.5326692865571871 1 35 1.1976312261237325 1 36 1.1660819938179543 1 37 2.171755186639267 1 38 1.1347463466669865 1 39 1.3636385153479296 1 40 4.034384725340733 1 41 -9.242951749398667 1 42 -8.82346288097633 1 43 -16.107627910286176 1 44 -21.89230673840182 1 45 -10.89169451327702 1 46 -17.430188920853972 1 47 -22.311144182704552 1 48 -13.98182325603155 1 49 -18.94692096883894 1 50 -12.339480869629128 1 51 -9.939914574847894 1 52 -9.185857316587104 1 53 -8.761559723826952 1 54 -8.69772945825542 1 55 -7.953022012036669 1 56 -3.484440108383108 2 1 1.290167760037093 2 2 1.5725383242550635 2 3 1.4864867599311324 2 4 1.8010455412276798 2 5 1.9819565022301475 2 6 4.038631291605917 2 7 1.832318942716796 2 8 1.8669909810428607 2 9 2.560897696152111 2 10 2.265134729777934 2 11 1.7588822830442652 2 12 -1.9645646242284016 2 13 -0.9234906603878414 2 14 2.2610341819879594 2 15 2.8610574732160714 2 16 1.980029087793568 2 17 3.266653532687826 2 18 -1.434517451940927 2 19 1.7302662356041694 2 20 2.9825989278631155 2 21 1.9635731916585684 2 22 2.6529982479132883 2 23 2.4083866082252214 2 24 1.2122254445644245 2 25 2.6213945955838085 2 26 3.136364472250264 2 27 2.0578689760050253 2 28 1.9426074716632868 2 29 2.583428103194116 2 30 2.6066081759710826 2 31 2.127908202131593 2 32 1.9311229261254255 2 33 1.581265623406722 2 34 1.3834650425253585 2 35 2.0033341438956143 2 36 2.0201933996534933 2 37 2.482763144198948 2 38 1.955701253828785 2 39 2.68790907237711 2 40 4.131864831441529 2 41 -9.906744852846945 2 42 -7.7956114220903885 2 43 -14.665320217978483 2 44 -15.784879682698906 2 45 -6.5919597652664095 2 46 -11.416926321384473 2 47 -17.273345774216487 2 48 -8.083282141973193 2 49 -12.916416990059096 2 50 -7.556324370955387 2 51 -2.0460153706038646 2 52 -1.1188811892661512 2 53 -1.1700477874874302 2 54 -0.24746420626603083 2 55 -0.7288840810021853 2 56 1.4751089632349306 3 1 0.9739042500010697 3 2 1.6710972537455433 3 3 1.6951846497921679 3 4 1.8916271161118772 3 5 2.226166486790106 3 6 4.1871130208905285 3 7 1.7744187883163889 3 8 2.2190239197973654 3 9 2.6594566256425907 3 10 2.5919489346158144 3 11 1.9320680782063846 3 12 3.912687048442727 3 13 3.509345160507678 3 14 3.495208139785177 3 15 2.6387208803184876 3 16 3.2775072144019077 3 17 3.6696386073146883 3 18 4.68514286715326 3 19 3.4502868223257366 3 20 3.5474471007915795 3 21 2.6365016528011296 3 22 3.213214408489719 3 23 3.0784843951526497 3 24 3.1090345027219115 3 25 3.104153216273467 3 26 1.4824787285549448 3 27 1.7395467938125364 3 28 1.2339095818022514 3 29 1.6917657254277714 3 30 1.5016158959916694 3 31 1.741649838775956 3 32 1.5489819070827053 3 33 1.0673695863506225 3 34 1.120212340518151 3 35 1.1801226153315412 3 36 1.8130395139098 3 37 2.4269216619549923 3 38 1.3442756233604385 3 39 2.595268825336454 3 40 6.372214805708129 3 41 6.186152728213276 3 42 3.5800962464633947 3 43 -2.3444246955904227 3 44 0.16854285976120664 3 45 2.4062388965966868 3 46 1.5537376003859826 3 47 -2.475610313588593 3 48 2.2986015430499656 3 49 3.8952145076248996 3 50 2.3479473737692658 3 51 4.091658329859339 3 52 3.9964044514955575 3 53 4.175294466758579 3 54 4.866277430378336 3 55 5.129067540202136 3 56 4.5996586287007055 4 1 1.5434005186577835 4 2 2.0267409104619603 4 3 1.6373488288966485 4 4 2.2444722653656086 4 5 2.3919780539542828 4 6 4.553717498502472 4 7 1.7919094599581769 4 8 2.2376806362152735 4 9 3.1088502823590076 4 10 3.310932143571037 4 11 3.838084869251162 4 12 5.074767272323321 4 13 5.1233843396121586 4 14 4.351551423367269 4 15 4.674635059422968 4 16 4.224102363655639 4 17 5.063528532687826 4 18 5.242745479093557 4 19 4.774447270086931 4 20 4.621607548552774 4 21 3.490512846830981 4 22 4.163774109982256 4 23 3.5791840220183246 4 24 3.1297900997368373 4 25 2.713528216273467 4 26 2.0550067136295738 4 27 1.971123286349851 4 28 2.463620402697771 4 29 2.2267470687113544 4 30 2.8302073139021147 4 31 2.2593737193729737 4 32 3.1175453399185287 4 33 2.7903173475446508 4 34 1.8559865942494937 4 35 2.3767177645852726 4 36 2.761033916894874 4 37 2.9698321097161866 4 38 2.966477115897753 4 39 3.6232538999633164 4 40 6.632942417648426 4 41 9.069548250601333 4 42 5.133267888254441 4 43 7.147179782021517 4 44 6.2399048000597155 4 45 6.12032471749221 4 46 5.6094745406844915 4 47 5.694865432680064 4 48 6.19365751319922 4 49 7.858367492699525 4 50 6.8924902842170255 4 51 5.914652732844413 4 52 6.841786914182126 4 53 7.029305660788431 4 54 8.24337631097535 4 55 7.265727987963331 4 56 6.207867583924585 5 1 1.8090255186577835 5 2 1.9954909104619603 5 3 2.2154738288966485 5 4 2.1038472653656086 5 5 2.5638530539542828 5 6 4.600592498502472 5 7 1.9325344599581769 5 8 2.3939306362152735 5 9 3.0151002823590076 5 10 2.873432143571037 5 11 3.556834869251162 5 12 5.340392272323321 5 13 5.1702593396121586 5 14 5.039051423367269 5 15 5.268385059422968 5 16 5.442852363655639 5 17 4.938528532687826 5 18 5.164620479093557 5 19 4.024447270086931 5 20 4.434107548552774 5 21 3.849887846830981 5 22 3.9606491099822563 5 23 3.3135590220183246 5 24 3.4266650997368373 5 25 3.791653216273467 5 26 3.2893817136295738 5 27 2.892998286349851 5 28 2.791745402697771 5 29 3.1954970687113544 5 30 3.0177073139021147 5 31 2.6968737193729737 5 32 2.9612953399185287 5 33 2.1028173475446508 5 34 1.1059865942494937 5 35 2.0954677645852726 5 36 3.323533916894874 5 37 3.4542071097161866 5 38 4.107102115897753 5 39 4.560753899963316 5 40 8.023567417648426 5 41 10.007048250601333 5 42 5.945767888254441 5 43 7.272179782021517 5 44 7.4742798000597155 5 45 6.30782471749221 5 46 5.5469745406844915 5 47 5.585490432680064 5 48 5.33428251319922 5 49 8.436492492699525 5 50 6.0018652842170255 5 51 5.586527732844413 5 52 5.998036914182126 5 53 5.685555660788431 5 54 6.02462631097535 5 55 5.640727987963331 5 56 7.067242583924585 6 1 1.8246505186577835 6 2 1.7277107380481667 6 3 1.624417794413887 6 4 2.128631748124228 6 5 2.0854047780922116 6 6 4.624838188157646 6 7 1.6254223909926608 6 8 1.7463013258704478 6 9 2.216070109945214 6 10 2.5065140401227595 6 11 3.034744352009781 6 12 4.914745720599186 6 13 5.0937507189225 6 14 5.157585906125888 6 15 4.533471266319523 6 16 4.738649777448742 6 17 5.1287224982050645 6 18 4.082723927369422 6 19 3.919921408017963 6 20 4.723978238207948 6 21 2.9635731916585684 6 22 2.773687903085701 6 23 3.3566624702941894 6 24 3.5398116514609725 6 25 2.431739423170022 6 26 1.9467092998364706 6 27 2.506144838073986 6 28 1.7184695406288029 6 29 2.1006694825044576 6 30 2.641090934591773 6 31 1.9727357883384897 6 32 2.172502236470251 6 33 1.805403554441206 6 34 2.0041546976977713 6 35 2.6067824197576854 6 36 3.089158916894874 6 37 3.051728661440322 6 38 3.4729426331391338 6 39 4.446529762032284 6 40 7.821520003855323 6 41 2.662220664394436 6 42 5.669905819288925 6 43 7.2657142647801365 6 44 6.249603075921787 6 45 6.080454027837035 6 46 6.324452988960356 6 47 6.036999053369723 6 48 5.623614409750942 6 49 8.566341630630557 6 50 6.771261835941161 6 51 5.833294974223723 6 52 7.243187776251094 6 53 7.278228074581534 6 54 6.85598406959604 6 55 7.44352971210126 6 56 5.785453790821137 7 1 1.5865552805625427 7 2 2.3856198787159286 7 3 1.4528250193728383 7 4 2.1420417098100515 7 5 2.1332974983987256 7 6 4.33447146675644 7 7 2.2266812853549993 7 8 2.0908552393898745 7 9 3.1041379807717036 7 10 2.3964976197615115 7 11 2.9846622502035416 7 12 4.4073565580376055 7 13 4.870160133262949 7 14 4.607503804319649 7 15 4.443980297518209 7 16 3.369191649369924 7 17 3.9491932152275098 7 18 4.694382383855462 7 19 3.5824829843726462 7 20 3.2986908818861096 7 21 3.2588660214341587 7 22 3.490162998871142 7 23 3.2064161648754705 7 24 3.126565893387628 7 25 3.469232581352834 7 26 1.8875959993438585 7 27 1.7833752704768315 7 28 1.6032533392057076 7 29 1.7867669099811962 7 30 1.9837291392989371 7 31 0.014334036833290043 7 32 1.30851756214075 7 33 0.4857538554811569 7 34 0.804647308535209 7 35 0.7638705423630512 7 36 0.7161430438790006 7 37 1.1305463954304713 7 38 1.8325485444691836 7 39 2.9665078682172847 7 40 4.524311465267477 7 41 -0.7389834954304142 7 42 3.414021856508409 7 43 5.517219464561201 7 44 4.789011942916861 7 45 3.997804876222368 7 46 4.459920969255922 7 47 4.601859480299115 7 48 3.7596297354214414 7 49 6.2844587625407975 7 50 3.6357938556455984 7 51 4.820158685225362 7 52 4.517134136404348 7 53 5.115119152851925 7 54 4.869120358594397 7 55 3.261263702249046 7 56 2.2277088537658543 8 1 1.6472311638190718 8 2 2.2238175233651845 8 3 1.6010585063160008 8 4 1.5846940395591602 8 5 2.1727240216962187 8 6 4.221056208179888 8 7 2.207736072861401 8 8 1.9392935394410813 8 9 2.6637897984880397 8 10 2.662242627442005 8 11 3.2004840627995463 8 12 5.0248680787749365 8 13 4.8184448234831265 8 14 5.145906262076949 8 15 4.770401188455224 8 16 4.825412847526607 8 17 5.444072887526538 8 18 4.950910801674205 8 19 4.610132753957899 8 20 4.546002709843094 8 21 2.6810370403793655 8 22 2.9561128196596727 8 23 2.934526763953805 8 24 3.3167860674787732 8 25 2.604153216273467 8 26 2.940591391048926 8 27 2.4121515121562993 8 28 3.2796486285042192 8 29 3.2680777138726427 8 30 3.4325258622892107 8 31 1.7452608161471659 8 32 2.5217792108862724 8 33 2.4133012185123945 8 34 1.3161680458623977 8 35 1.1796411516820484 8 36 2.508513755604554 8 37 2.8125740452000585 8 38 2.5652674384784007 8 39 2.127286158027836 8 40 3.78314403055165 8 41 -1.0252098139147954 8 42 3.736090468899601 8 43 5.463712040086037 8 44 5.95260641296294 8 45 4.161655362653498 8 46 5.257155992297395 8 47 6.715530755260712 8 48 4.876117190618572 8 49 6.498488460441461 8 50 5.241784639055737 8 51 5.803261603812157 8 52 4.778782881924062 8 53 6.348862112401335 8 54 6.144081956136638 8 55 5.611494116995587 8 56 5.522383712956845 9 1 1.4687183152679495 9 2 1.841094724021282 9 3 1.4996369644898664 9 4 1.9348854009588266 9 5 2.2990225454797084 9 6 4.4509632612143335 9 7 2.1300980192802115 9 8 2.3012399582491696 9 9 2.37182697727426 9 10 2.7677647706896806 9 11 3.129133598064719 9 12 4.969099899441964 9 13 3.9578652718155496 9 14 5.3112971860791305 9 15 4.5448681102704285 9 16 4.812290922977674 9 17 4.215806075060705 9 18 5.043857767229149 9 19 4.274976931103879 9 20 4.033154158722269 9 21 3.4910425078479292 9 22 2.7538164828636127 9 23 3.639830208459003 9 24 3.29901679465209 9 25 3.8160176230531277 9 26 2.9069664593922866 9 27 2.7484008287227297 9 28 2.9887793010028574 9 29 2.979395373796102 9 30 3.480366212207201 9 31 2.51043304140687 9 32 2.6117190687320857 9 33 1.8930715848327893 9 34 1.5310395603511893 9 35 1.3928724256022207 9 36 3.470514849098265 9 37 2.9126287198856815 9 38 2.166688980304535 9 39 3.896558984709081 9 40 6.544489027817921 9 41 4.4307770641606545 9 42 4.428818735712067 9 43 6.80078147693677 9 44 6.154629376330899 9 45 4.906871327661705 9 46 4.878807167803135 9 47 5.6559353479342995 9 48 4.554356665741594 9 49 7.709532746936812 9 50 4.759280538454313 9 51 4.766082817590178 9 52 5.667793270114327 9 53 6.57220820316131 9 54 5.82150131097535 9 55 5.767316971014175 9 56 5.2460031771449245 10 1 1.4280987945198547 10 2 1.434607289772302 10 3 1.952004001310442 10 4 2.076907610193196 10 5 1.9302323642991084 10 6 4.107596808847298 10 7 1.3495603220271448 10 8 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1.468853053954284 116 6 4.146217498502473 116 7 0.9812844599581751 116 8 1.0476806362152757 116 9 1.6257252823590065 116 10 2.2603071435710405 116 11 -2.9162901307488376 116 12 -13.558357727676679 116 13 -8.64349066038784 116 14 -2.933448576632731 116 15 -6.234114940577033 116 16 -2.576522636344361 116 17 -1.233346467312174 116 18 -6.550379520906443 116 19 -2.3918027299130706 116 20 -2.1346424514472275 116 21 2.2042628468309786 116 22 2.821274109982255 116 23 2.3435590220183258 116 24 2.4129150997368356 116 25 2.7241532162734643 116 26 1.661881713629576 116 27 1.9592482863498475 116 28 1.5667454026977694 116 29 2.9254970687113513 116 30 2.1183323139021155 116 31 2.4368737193729757 116 32 2.946295339918528 116 33 2.1778173475446536 116 34 2.630361594249493 116 35 2.6860927645852755 116 36 3.029158916894872 116 37 2.5848321097161886 116 38 2.0839771158977527 116 39 4.458253899963317 116 40 6.656692417648426 116 41 2.9070482506013313 116 42 -3.274232111745558 116 43 -10.425320217978484 116 44 -10.593845199940283 116 45 -3.1009252825077915 116 46 -5.988650459315508 116 47 -4.385759567319937 116 48 -5.762592486800781 116 49 -9.525382507300474 116 50 -5.655634715782973 116 51 -3.1922222671555858 116 52 -2.805088085817875 116 53 -5.296944339211571 116 54 -8.298498689024651 116 55 -3.6930220120366677 116 56 -2.902132416075414 117 1 0.33893623294349595 117 2 0.7887944818905339 117 3 0.20520597175379152 117 4 1.211882979651321 117 5 0.6602816253828507 117 6 3.6519317842167567 117 7 0.7155701742438936 117 8 0.8591092076438471 117 9 0.6057252823590105 117 10 1.063164286428183 117 11 -2.4248615593202665 117 12 -8.421214870533824 117 13 -10.437776374673557 117 14 -11.97344857663273 117 15 -8.908400654862746 117 16 -16.802236922058647 117 17 -13.961917895883602 117 18 -13.867522378049301 117 19 -4.903231301341641 117 20 -5.948928165732941 117 21 0.027119989688124235 117 22 0.8012741099822591 117 23 0.2921304505897524 117 24 -0.15279918597745024 117 25 0.10415321627346685 117 26 1.5733102850581417 117 27 1.656391143492705 117 28 1.7524596884120598 117 29 1.2597827829970711 117 30 1.844046599616398 117 31 1.354016576515832 117 32 1.4862953399185272 117 33 2.0349602046875077 117 34 1.3475044513923535 117 35 1.3860927645852712 117 36 2.446301774037728 117 37 2.7591178240018976 117 38 3.261119973040607 117 39 3.312539614249033 117 40 6.819549560505568 117 41 6.149905393458479 117 42 -1.411374968888417 117 43 -6.3367487894070536 117 44 -6.753845199940283 117 45 0.23907471749220832 117 46 -2.0829361736012224 117 47 -2.6171881387485065 117 48 -2.46259248680078 117 49 -6.4425253644433305 117 50 -3.335634715782973 117 51 -1.5036508385841572 117 52 -1.8765166572464445 117 53 -1.948372910640142 117 54 -8.97849868902465 117 55 -1.1673077263223846 117 56 0.5007247267817299 118 1 0.6708043648116302 118 2 0.9301864232824713 118 3 1.1341437006915172 118 4 1.1334947012630465 118 5 1.2529556180568449 118 6 3.5457046779896544 118 7 1.0935921522658703 118 8 0.9748601233947625 118 9 1.1493150259487521 118 10 1.5613327845966793 118 11 -1.1142388486975534 118 12 -4.980921830240781 118 13 -4.564516301413484 118 14 -5.234987038171191 118 15 -2.794114940577032 118 16 -11.019086738908463 118 17 -9.502577236542944 118 18 -12.51807182859875 118 19 -5.208725806836146 118 20 -3.162847579652354 118 21 -0.04394228137415013 118 22 0.24229975100789858 118 23 0.37125132971063124 118 24 0.5124022792240197 118 25 1.1041532162734669 118 26 2.140343252091114 118 27 2.363350850452413 118 28 2.5898223257746906 118 29 1.9967791199934055 118 30 2.7224348780046768 118 31 1.4917455142447693 118 32 -6.785499531876344 118 33 2.3624327321600376 118 34 0.9621564660443624 118 35 1.5245543030468127 118 36 1.973774301510261 118 37 2.434575699459778 118 38 3.1988489107695486 118 39 4.434151335860754 118 40 6.527974468930477 118 41 4.955766199319282 118 42 -1.6055141630276104 118 43 -4.549935602593866 118 44 -4.558973405068489 118 45 0.42881830723580094 118 46 -7.122496613161662 118 47 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29 0.6211727443870316 119 30 1.3475215030913006 119 31 -0.0058289833297280325 119 32 -4.697488443865254 119 33 0.9507903205176262 119 34 -0.2609897571018607 119 35 0.6347414132339182 119 36 0.1837535114894706 119 37 0.19618346106754103 119 38 0.4310041429247775 119 39 1.9982538999633164 119 40 4.6929086338646435 119 41 -1.8307895872365059 119 42 -6.310988868502317 119 43 -15.273428326086592 119 44 -13.732223578318663 119 45 -8.404168525751032 119 46 -18.671353162018214 119 47 -17.283597405157774 119 48 -16.435565459773755 119 49 -16.093490615408584 119 50 -14.827526607674866 119 51 -11.91600605093937 119 52 -11.840223220953009 119 53 -12.91748487975211 119 54 -14.90822841875438 119 55 -12.047616606631264 119 56 -9.71780809175109 120 1 -0.3688978684389923 120 2 -0.0987631217961038 120 3 -0.060231816264639804 120 4 -0.5604672507634234 120 5 0.26949821524460305 120 6 2.4629916920508563 120 7 -0.5019413464934388 120 8 -0.2865129121718226 120 9 -0.4007263305442166 120 10 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-14.026404728610258 120 48 -10.52710861583304 120 49 -16.049898636332735 120 50 -9.580796006105555 120 51 -6.261254525220103 120 52 -6.705088085817874 120 53 -6.796299177921245 120 54 -12.339789011605296 120 55 -7.291731689456025 120 56 -4.526003383817351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 306.0 5 37.0 6 25.0 7 41.0 8 67.0 9 49.0 10 64.0 11 47.0 12 61.0 13 59.0 14 72.0 15 112.0 16 169.0 17 204.0 18 241.0 19 312.0 20 372.0 21 493.0 22 584.0 23 699.0 24 789.0 25 926.0 26 933.0 27 1129.0 28 1350.0 29 1495.0 30 1753.0 31 2138.0 32 2462.0 33 2811.0 34 3166.0 35 3832.0 36 4940.0 37 6029.0 38 5135.0 39 2591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.36253929175916 0.5099685665926625 0.06374607082408282 0.06374607082408282 8 99.28120809794913 0.4923834436067087 0.14727540500736377 0.07913305343679247 9 97.88538896093904 1.3430637680522277 0.4308355131558701 0.34071175785285646 10 48.15026487591498 34.561361088519114 10.14661596289539 7.1417580726705205 11 34.27120655925087 28.45053085090014 20.737256281186117 16.54100630866287 12 35.51095772976062 14.472556217440047 22.750752863077835 27.265733189721498 13 25.16982874227801 17.059819289027633 26.28223448457801 31.48811748411634 14 27.37783834875695 18.275339063152572 29.138548787725583 25.20827380036489 15 27.52317822458103 20.20084124292541 26.536589662842168 25.73939086965139 16 32.95287106067604 19.924787227023817 25.38760968529392 21.734732027006224 17 31.600466005759127 20.869144703580773 24.038863121798958 23.491526168861142 18 28.86441572144553 22.836982326562914 24.76699199859316 23.531609953398398 19 29.631747987152977 27.194333230674467 21.58695939108628 21.58695939108628 20 29.416035909170922 24.445520154902304 21.886551663439533 24.25189227248724 21 28.749477941661354 21.71982502802629 26.968984239333523 22.561712790978834 22 26.105115072648537 22.388059701492537 26.50517662057899 25.001648605279936 23 27.650407755039236 25.054403974237793 23.61242388938958 23.682764381333392 24 27.415206735102103 23.539885257072516 26.44143054975491 22.603477458070472 25 27.692781148333772 24.74940648905302 24.29877780708696 23.259034555526245 26 25.207183838561477 21.606471609768967 28.478160515266755 24.708184036402805 27 24.487371292792396 19.979318841855143 29.12743113614362 26.405878729208837 28 23.373165203230563 24.502101626284635 29.094870271340863 23.029862899143943 29 25.87844672149171 22.498790624037994 30.300365011653984 21.322397642816306 30 23.414451845501112 18.481391105542 37.24416891995867 20.859988128998218 31 22.623916963539607 21.28249109381185 35.42683731362977 20.66675462901878 32 28.554460672180188 18.883072320957243 34.044518740102056 18.517948266760513 33 24.12312570182971 20.809388554946388 35.314970165356584 19.752515577867317 34 19.66830899172972 21.295970438148863 39.8183177898997 19.217402780221715 35 21.35347937010645 22.171637195390165 40.58678631125187 15.888097123251518 36 22.38960239268121 24.05436312456017 37.20751231527093 16.348522167487683 37 22.76220465505472 21.52026952634708 37.52229537797547 18.19523044062273 38 22.745608041087134 22.787488703242445 37.61544735159918 16.85145590407124 39 22.601880187578434 21.994231743026354 36.23213931881729 19.171748750577926 40 17.06178955283294 15.489406873369013 33.869255606174534 33.57954796762351 41 15.118082325313484 15.21334573973149 32.3873454738801 37.28122646107492 42 19.277851166886833 20.779392338177015 37.04315279612506 22.899603698811095 43 16.49157581764123 17.86146900121132 39.489043056932054 26.157912124215393 44 17.68187640796855 17.690710720438183 35.92914881399355 28.698264057599715 45 20.146738052746382 21.36294533677044 38.93846255535726 19.551854055125915 46 19.901790236275957 20.77819126681787 38.23794948583005 21.082069011076126 47 18.088662151464508 21.411293869293694 40.117864368018296 20.382179611223503 48 20.202998745018604 21.91153482022942 39.948039366784826 17.937427067967153 49 16.915532779643048 18.163544795900336 33.672026859869234 31.248895564587382 50 19.792194069606182 20.76738503533141 39.701058841658046 19.739362053404363 51 20.49993387118106 22.428691090243795 37.922673367720314 19.148701670854827 52 18.34529207201655 22.984989215125236 39.25694413875072 19.412774574107498 53 20.755173932188463 20.889476001761338 39.502421840598856 18.852928225451343 54 17.69266348038676 21.458934431645485 37.71556945576284 23.13283263220492 55 19.1030303030303 22.64022038567493 37.498622589531685 20.758126721763084 56 22.5568382093761 21.98625308424392 35.70673246387028 19.750176242509692 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 12.5 13 48.0 14 72.0 15 103.0 16 134.0 17 143.5 18 153.0 19 207.5 20 262.0 21 302.0 22 380.0 23 418.0 24 509.5 25 601.0 26 712.0 27 823.0 28 995.0 29 1279.5 30 1392.0 31 1539.0 32 1686.0 33 1648.5 34 1611.0 35 1555.0 36 1499.0 37 1296.5 38 997.0 39 900.0 40 886.0 41 872.0 42 873.0 43 874.0 44 948.5 45 1023.0 46 1119.5 47 1393.5 48 1571.0 49 1646.5 50 1722.0 51 1761.0 52 1800.0 53 1774.0 54 1899.5 55 2051.0 56 2068.5 57 2086.0 58 2203.0 59 2320.0 60 2413.0 61 2506.0 62 2544.0 63 2672.0 64 2762.0 65 2500.0 66 2238.0 67 1989.5 68 1741.0 69 1550.5 70 1360.0 71 1120.5 72 772.0 73 663.0 74 587.5 75 512.0 76 428.5 77 345.0 78 277.5 79 167.0 80 124.0 81 95.5 82 67.0 83 46.0 84 25.0 85 18.5 86 12.0 87 7.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.013188842239465412 14 0.0 15 0.18464379135251577 16 0.04835908821137318 17 0.0 18 0.0021981403732442353 19 0.07693491306354824 20 0.09891631679599058 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0021981403732442353 26 0.004396280746488471 27 0.09012375530301364 28 0.11430329940870024 29 0.03297210559866353 30 0.008792561492976941 31 0.04176466709164047 32 0.06374607082408282 33 0.16705866836656189 34 0.06374607082408282 35 0.05495350933110588 36 0.04616094783812894 37 0.17585122985953883 38 0.27696568702877367 39 0.15826610687358494 40 0.6044886026421648 41 0.7803398325017036 42 0.16046424724682917 43 0.1934363528454927 44 0.4726001802475106 45 0.23300287956388893 46 0.17585122985953883 47 0.037368386345152 48 0.1626623876200734 49 0.48578902248697603 50 0.14507726463411952 51 0.2791638274020179 52 0.1296902820214099 53 0.16046424724682917 54 0.19783263359198117 55 0.2593805640428198 56 0.22201217769766776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 45493.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.93036291297562 #Duplication Level Percentage of deduplicated Percentage of total 1 86.77300755580002 65.88705954762271 2 7.894508294010364 11.98865759567406 3 2.5880786266392612 5.895412481041039 4 1.0653388530237675 3.2356626294155144 5 0.4747705758040703 1.802475106060273 6 0.3097588512868019 1.4112061196227992 7 0.2229105752250818 1.1847976611786428 8 0.12448252902179892 0.756160288396017 9 0.11290275888023622 0.7715472710087266 >10 0.4052919549546941 5.06011913920823 >50 0.02605448281851605 1.4309893829819973 >100 0.0028949425353906725 0.5759127777899896 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 262 0.5759127777899896 No Hit TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 91 0.20003077396522542 No Hit TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 86 0.18904007209900425 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 81 0.17804937023278306 No Hit TGCGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 80 0.17585122985953883 No Hit TGCGGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 74 0.1626623876200734 No Hit TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 64 0.14068098388763106 No Hit TGCGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 61 0.13408656276789835 No Hit TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 60 0.13188842239465412 No Hit TGCGGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 54 0.11869958015518871 No Hit TGCGGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 48 0.1055107379157233 No Hit TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.10111445716923483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.006594421119732706 0.0 0.0 0.0 0.0 7 0.006594421119732706 0.0 0.0 0.0 0.0 8 0.006594421119732706 0.0 0.0 0.0 0.0 9 0.006594421119732706 0.0 0.0 0.0 0.0 10 0.015386982612709647 0.0 0.0 0.0 0.0 11 0.026377684478930824 0.0 0.0 0.0 0.0 12 0.02857582485217506 0.0 0.0 0.0 0.0 13 0.039566526718396236 0.0 0.0 0.0 0.0 14 0.04396280746488471 0.0 0.0 0.0 0.0 15 0.04835908821137318 0.0 0.0 0.0 0.0 16 0.05055722858461741 0.0 0.0 0.0 0.0 17 0.06594421119732706 0.0 0.0 0.0 0.0 18 0.07253863231705977 0.0 0.0 0.0 0.0 19 0.08133119381003671 0.0 0.0 0.0 0.0 20 0.09232189567625788 0.0 0.0 0.0 0.0 21 0.09452003604950213 0.0 0.0 0.0 0.0 22 0.09671817642274635 0.0 0.0 0.0 0.0 23 0.10770887828896754 0.0 0.0 0.0 0.0 24 0.12089772052843295 0.0 0.0 0.0 0.0 25 0.14068098388763106 0.0 0.0 0.0 0.0 26 0.14507726463411955 0.0 0.0 0.0 0.0 27 0.15606796650034072 0.0 0.0 0.0 0.0 28 0.16266238762007343 0.0 0.0 0.0 0.0 29 0.1692568087398061 0.0 0.0 0.0 0.0 30 0.1692568087398061 0.0 0.0 0.0 0.0 31 0.1736530894862946 0.0 0.0 0.0 0.0 32 0.17585122985953883 0.0 0.0 0.0 0.0 33 0.17804937023278306 0.0 0.0 0.0 0.0 34 0.17804937023278306 0.0 0.0 0.0 0.0 35 0.1802475106060273 0.0 0.0 0.0 0.0 36 0.18244565097927154 0.0 0.0 0.0 0.0 37 0.18464379135251577 0.0 0.0 0.0 0.0 38 0.18464379135251577 0.0 0.0 0.0 0.0 39 0.18464379135251577 0.0 0.0 0.0 0.0 40 0.18684193172576 0.0 0.0 0.0 0.0 41 0.1934363528454927 0.0 0.0 0.0 0.0 42 0.1934363528454927 0.0 0.0 0.0 0.0 43 0.1978326335919812 0.0 0.0 0.0 0.0 44 0.20222891433846965 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCCA 15 0.008074136 50.47046 39 CCACTGA 15 0.008074136 50.47046 40 TCGTCTC 15 0.008074136 50.47046 40 AAGAGCC 35 4.8574293E-8 50.35818 35 TAAGACC 15 0.008145647 50.358173 35 ATGCTCG 15 0.008145647 50.358173 36 CAGCGTA 15 0.008145647 50.358173 36 AGCGTAG 15 0.008145647 50.358173 37 GTACGCC 15 0.008145647 50.358173 37 ATATGCC 25 1.9833864E-5 50.302223 34 GTAAGAC 15 0.0081815785 50.30222 34 TTGTTGG 15 0.008253796 50.19069 49 AACGATT 30 9.988726E-7 50.19069 47 CCGCTCT 15 0.008253796 50.19069 48 ACCGCTC 15 0.008253796 50.19069 47 GACCGCT 15 0.008253796 50.19069 46 TCTGAGT 80 0.0 50.19069 48 ACGATTC 15 0.008253796 50.19069 48 CAACGAT 30 9.988726E-7 50.19069 46 CGATTCA 15 0.008253796 50.19069 49 >>END_MODULE