FastQCFastQC Report
Fri 17 Jun 2016
SRR1526212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110557
Sequences flagged as poor quality0
Sequence length56
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG52424.741445589153106No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG14481.3097316316470238No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG14121.2771692430149153No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG11841.0709407816782293No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG3650.33014644029776496No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3480.31476975677704716No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3060.27678030337292076No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG3030.27406677098691173No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2990.2704487278055664No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG2650.23969536076413073No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2150.1944698209973136No Hit
TGATCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1880.17004802952323236No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG1520.13748564089112403No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1280.11577738180305183No Hit
TGATCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1180.10673227384968839No Hit
TGATCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG1120.10130520907767034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTC203.96809E-450.49839438
ACCCACA203.96809E-450.49839439
CGTCTCA2200.050.4752341
CTCAATG252.0050555E-550.29067644
CAAAAAC252.0050555E-550.29067643
CGCTGAA150.00822024650.29067637
TGCTCGT1900.050.29067637
GTGAACT204.0496918E-450.29067644
ATGCTCG1900.050.2218236
GAACTCT204.0863844E-450.19890646
ACGATTC1300.050.19890648
AATGATT301.011078E-650.19890647
TGAGGGT150.00827984350.19890648
AACTCTG204.0863844E-450.19890647
TTAAGAC204.0955987E-450.17601434
ACCCGCT252.0323412E-550.17601434
TGATATC150.00841511149.9936415
ATTCCTT150.00841511149.9936414
CCAGTCA150.00841511149.9936450
CCTGGTA204.1886535E-449.94825413
Produced by FastQC (version 0.11.5)