FastQCFastQC Report
Fri 17 Jun 2016
SRR1526211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69051
Sequences flagged as poor quality0
Sequence length56
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9101.3178665044677123No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2510.3634994424410942No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2400.3475691879914845No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1950.2823999652430812No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1730.2505394563438618No Hit
GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1640.23750561179418112No Hit
GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1480.2143343325947488No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT730.10571896134740988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGTC309.811174E-750.3772938
TGCCCGT150.00817510150.34041237
ACTGTGC150.00817510150.34041241
TATGCTT2050.050.26681535
CGATTCA252.0027068E-550.2668149
CGATTAA402.4992914E-950.2668149
TATGCCT309.961168E-750.2668135
TATGCCC150.00822256450.2668135
ACGATTC252.0113976E-550.23009548
ACGATTA402.5156623E-950.23009548
AACGATT600.050.23009547
AAGATTA2400.050.23009548
ATATGCC550.050.15682234
ATATGCT2800.050.1568234
AATGAGG150.00831810550.12026243
ATGAGGT150.00831810550.12026244
ATAAACA150.00831810550.12026250
GATTCAG252.037658E-550.12026250
TAATGAG150.00834211850.0837642
GGATAAG150.008390350.0109132