##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526211_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69051 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.20376243646001 39.0 38.0 39.0 35.0 40.0 2 37.89203632098015 39.0 38.0 39.0 35.0 40.0 3 37.892702495257126 39.0 38.0 39.0 35.0 40.0 4 38.05878263891906 39.0 38.0 39.0 35.0 40.0 5 38.01771154653807 39.0 38.0 39.0 35.0 40.0 6 38.07700105718961 39.0 38.0 39.0 35.0 40.0 7 37.65487827837396 39.0 38.0 39.0 35.0 40.0 8 37.765072193016756 39.0 38.0 39.0 35.0 40.0 9 37.84217462455287 39.0 38.0 39.0 35.0 40.0 10 37.526031483975615 39.0 38.0 39.0 35.0 40.0 11 36.46284630200866 38.0 36.0 39.0 31.0 40.0 12 34.690851689331076 38.0 34.0 39.0 23.0 40.0 13 34.34945185442644 38.0 33.0 39.0 23.0 40.0 14 34.44781393462803 38.0 33.0 39.0 23.0 40.0 15 34.36500557558906 38.0 33.0 39.0 23.0 40.0 16 33.85577326903304 38.0 33.0 39.0 22.0 40.0 17 33.77681713516097 38.0 32.0 39.0 22.0 40.0 18 33.21547841450522 37.0 31.0 39.0 19.0 40.0 19 34.07411912933918 38.0 32.0 39.0 24.0 40.0 20 34.118622467451594 37.0 32.0 39.0 25.0 40.0 21 34.73833832964041 38.0 33.0 39.0 26.0 40.0 22 34.44384585306513 38.0 33.0 39.0 25.0 40.0 23 34.9385961101215 38.0 34.0 39.0 26.0 40.0 24 34.529188570766536 38.0 33.0 39.0 25.0 40.0 25 34.80210279358735 38.0 33.0 39.0 26.0 40.0 26 35.292928415229326 38.0 35.0 39.0 28.0 40.0 27 35.18954106385136 38.0 35.0 39.0 28.0 40.0 28 35.103952151308455 38.0 35.0 39.0 27.0 40.0 29 34.9225065531274 38.0 35.0 39.0 27.0 40.0 30 34.78602771864274 38.0 34.0 39.0 26.0 40.0 31 35.08920942491781 38.0 35.0 39.0 28.0 40.0 32 34.7632619368293 38.0 35.0 39.0 27.0 40.0 33 34.90644596023229 38.0 35.0 39.0 27.0 40.0 34 34.74964881029963 38.0 34.0 39.0 27.0 40.0 35 34.56843492491058 38.0 34.0 39.0 27.0 40.0 36 34.22121330610708 38.0 33.0 39.0 27.0 40.0 37 33.964272783884375 37.0 33.0 38.0 26.0 40.0 38 33.7019159751488 37.0 33.0 38.0 25.0 39.0 39 33.12624002548841 36.0 32.0 38.0 22.0 39.0 40 30.065777468827388 35.0 26.0 38.0 10.0 39.0 41 26.91105125197318 32.0 16.0 38.0 6.0 39.0 42 30.825578195826274 35.0 29.0 38.0 11.0 39.0 43 29.472752023866416 34.0 26.0 38.0 6.0 39.0 44 29.3877423933035 34.0 26.0 38.0 4.0 39.0 45 30.407061447336027 35.0 29.0 38.0 4.0 39.0 46 29.972672372594168 35.0 27.0 38.0 4.0 39.0 47 29.446235391232566 35.0 26.0 38.0 4.0 39.0 48 29.52933339126153 35.0 27.0 38.0 4.0 39.0 49 27.0654009355404 33.0 16.0 38.0 4.0 39.0 50 29.103503207773965 35.0 26.0 38.0 4.0 39.0 51 29.341037783667144 35.0 27.0 39.0 4.0 40.0 52 29.141837192799525 35.0 26.0 38.0 4.0 40.0 53 28.71393607623351 35.0 24.0 38.0 4.0 40.0 54 27.679222603582858 34.0 18.0 38.0 4.0 40.0 55 28.014626869994643 34.0 22.0 38.0 4.0 39.0 56 26.74815715920117 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.22316520183237998 1 2 0.1351136908854187 1 3 -0.11854001927753188 1 4 -0.0756015130848624 1 5 -0.12518969407555858 1 6 -0.08939161138248863 1 7 0.28648346813957914 1 8 0.22256760066861858 1 9 -0.017435492743402392 1 10 -0.6322765266788011 1 11 -3.051773298660507 1 12 -12.7887366815897 1 13 -10.077621669784019 1 14 -5.732705490184401 1 15 -6.899903862661503 1 16 -23.113280676466232 1 17 -5.369528458458255 1 18 -14.837537555924417 1 19 -6.045804604707868 1 20 -5.947564221662571 1 21 -6.409609455900604 1 22 -2.3319102047195237 1 23 -3.258577603039921 1 24 -4.163478956949991 1 25 -3.8889119456405403 1 26 1.0300828738080696 1 27 1.331608647426691 1 28 1.136400268620008 1 29 1.3440316217115438 1 30 1.5712967090994212 1 31 1.1928210025726145 1 32 1.2637646329511867 1 33 0.9946589669833159 1 34 1.1605259060022561 1 35 1.5342983458310826 1 36 1.5348798338607565 1 37 2.1636117921684033 1 38 1.828092533500211 1 39 1.9537867727439462 1 40 4.7440092695457174 1 41 -9.585315573679399 1 42 -7.334091913512687 1 43 -16.552946318668155 1 44 -22.00905235248553 1 45 -9.792779020357273 1 46 -15.82772098770123 1 47 -22.784863766265545 1 48 -12.646297824346826 1 49 -19.45270790786458 1 50 -12.37099085057517 1 51 -8.226347791946203 1 52 -8.246681475856676 1 53 -8.087623581622303 1 54 -7.205510269841298 1 55 -7.495474262134504 1 56 -1.7783423593467695 2 1 0.4531762615822572 2 2 1.1134063738122464 2 3 1.1560941270639304 2 4 0.8551301942322098 2 5 1.0506639644610303 2 6 0.9532913154467764 2 7 1.2423371266761691 2 8 1.4750066250588674 2 9 1.2525645072566007 2 10 0.8935771318577821 2 11 1.6533486525590035 2 12 -1.1460537547604304 2 13 -0.5749387429547497 2 14 3.552416461035115 2 15 3.0403400397775187 2 16 2.7920851771923054 2 17 3.189252029346619 2 18 -0.4090009705585622 2 19 2.555414907487254 2 20 2.7897528515081618 2 21 1.9169759099530523 2 22 2.831748331865846 2 23 2.568983372569832 2 24 1.589691774757327 2 25 2.1147465909448258 2 26 2.682521898198317 2 27 2.2701452327925438 2 28 2.518595390571228 2 29 2.5408608900042324 2 30 2.6737357334896714 2 31 2.36111368549944 2 32 0.8327890231950903 2 33 1.288805308446726 2 34 1.9902820035632303 2 35 1.5555178580262066 2 36 1.6763432484949021 2 37 1.5526361824123072 2 38 2.107848631061188 2 39 1.890859943475654 2 40 4.014009269545721 2 41 -5.1426326468501316 2 42 -6.0040919135126884 2 43 -12.468799977204743 2 44 -14.329296254924554 2 45 -5.328144874015809 2 46 -9.907477085262204 2 47 -16.880961327241153 2 48 -7.844590507273654 2 49 -12.332951810303603 2 50 -7.624161582282486 2 51 -3.25512827975108 2 52 -3.252779036832287 2 53 -3.1829894352808417 2 54 -1.472583440573004 2 55 -2.6681571889637716 2 56 1.677511299189817 3 1 0.30317626158225863 3 2 0.9234063738122487 3 3 0.8260941270639321 3 4 0.4351301942322152 3 5 0.9506639644610289 3 6 0.9932913154467755 3 7 0.982337126676164 3 8 1.0850066250588668 3 9 1.052564507256598 3 10 0.5835771318577869 3 11 1.9033486525590035 3 12 3.5839462452395665 3 13 2.4150612570452523 3 14 3.062416461035113 3 15 3.160340039777523 3 16 3.6220851771923037 3 17 3.929252029346621 3 18 4.24099902944144 3 19 3.555414907487254 3 20 3.409752851508159 3 21 3.10697590995305 3 22 2.301748331865845 3 23 3.3589833725698384 3 24 2.7596917747573286 3 25 2.8047465909448306 3 26 2.472521898198316 3 27 2.550145232792545 3 28 3.1085953905712316 3 29 2.690860890004231 3 30 2.063735733489672 3 31 1.3411136854994439 3 32 2.1427890231950855 3 33 1.3488053084467282 3 34 1.7002820035632311 3 35 1.665517858026206 3 36 2.7763432484949035 3 37 2.752636182412303 3 38 2.7778486310611896 3 39 3.3308599434756587 3 40 6.904009269545714 3 41 8.197367353149872 3 42 4.615908086487316 3 43 -0.8087999772047425 3 44 3.3707037450754456 3 45 4.541855125984192 3 46 3.6025229147377935 3 47 -2.0109613272411515 3 48 2.9654094927263444 3 49 5.327048189696399 3 50 2.455838417717512 3 51 4.7748717202489175 3 52 4.397220963167712 3 53 5.067010564719162 3 54 6.157416559426995 3 55 4.671842811036228 3 56 4.387511299189818 4 1 0.32317626158225465 4 2 1.1718274264438264 4 3 0.7908309691691926 4 4 0.179340720547998 4 5 0.861716596039976 4 6 0.982238683867827 4 7 1.115495021413011 4 8 0.909217151374655 4 9 0.8720381914671265 4 10 0.8914718686998881 4 11 2.1538749683484824 4 12 3.867104139976412 4 13 3.772955993887358 4 14 3.304521724193009 4 15 3.7850768818827873 4 16 4.456295703508097 4 17 4.337146766188724 4 18 4.978893766283541 4 19 4.126467539066205 4 20 4.09659495677132 4 21 3.3375022257425258 4 22 3.3112220160763712 4 23 2.8810886357277283 4 24 3.4939023010731134 4 25 2.9857992225237737 4 26 1.8430482139877924 4 27 2.195408390687277 4 28 2.343332232676495 4 29 2.3287556268463376 4 30 2.2579462598054576 4 31 1.619008422341544 4 32 2.3238416547740357 4 33 2.176173729499361 4 34 1.6755451614579684 4 35 1.7539389106577872 4 36 1.892132722179113 4 37 2.150004603464936 4 38 2.664164420534867 4 39 2.844544154001973 4 40 5.844009269545719 4 41 8.42315682683408 4 42 5.3448554549083624 4 43 6.3706737070057855 4 44 6.190177429285971 4 45 5.769223547036823 4 46 4.2941018621062135 4 47 3.469564988548324 4 48 5.080146334831607 4 49 7.683363979170082 4 50 5.308469996664879 4 51 4.851187509722607 4 52 4.576168331588768 4 53 4.548063196298109 4 54 5.624784980479628 4 55 4.801316495246752 4 56 6.73487972024245 5 1 0.5862197398431235 5 2 1.1425368085948548 5 3 1.0569636922813217 5 4 0.7581736724930792 5 5 0.9363161383740746 5 6 0.8445956632728624 5 7 0.9458153875457285 5 8 1.2771805381023427 5 9 1.1564775507348557 5 10 0.46183800142300413 5 11 2.132479087341615 5 12 4.53655494089174 5 13 4.019409083132203 5 14 4.375459939295979 5 15 4.66425308325578 5 16 4.863389525018391 5 17 4.90751289891184 5 18 5.516651203354485 5 19 4.4054149074872555 5 20 4.259752851508161 5 21 3.5508889534313113 5 22 4.16305267969193 5 23 3.273765981265484 5 24 3.99664829649646 5 25 3.3017031126839598 5 26 1.932521898198317 5 27 2.40318871105341 5 28 1.8007693036147074 5 29 2.6186869769607526 5 30 2.821561820446192 5 31 1.8850267289776994 5 32 2.1810498927603064 5 33 1.1831531345336828 5 34 2.172455916606708 5 35 2.072474379765339 5 36 3.267647596320991 5 37 2.4504622693688276 5 38 2.872631239756835 5 39 3.5969468999973984 5 40 6.602704921719631 5 41 9.087802135758565 5 42 6.100690695182962 5 43 6.7442435010561255 5 44 6.481138527684141 5 45 5.556637734679839 5 46 6.084262045172576 5 47 6.415560411889285 5 48 6.933235579682869 5 49 9.591830798392046 5 50 6.936273200326209 5 51 5.627045633292397 5 52 5.932003571863362 5 53 6.468314912545246 5 54 6.629590472470472 5 55 5.244451506688399 5 56 4.457076516581122 6 1 0.7331762615822583 6 2 1.4234063738122487 6 3 1.2060941270639276 6 4 0.9551301942322112 6 5 1.1906639644610308 6 6 1.2832913154467747 6 7 1.3023371266761643 6 8 1.2450066250588634 6 9 1.2725645072565968 6 10 0.8635771318577881 6 11 2.3933486525590055 6 12 4.473946245239567 6 13 4.665061257045252 6 14 4.742416461035113 6 15 4.580340039777525 6 16 5.192085177192304 6 17 5.0292520293466225 6 18 5.790999029441437 6 19 4.535414907487258 6 20 4.669752851508157 6 21 3.826975909953056 6 22 3.861748331865847 6 23 3.998983372569832 6 24 3.659691774757327 6 25 3.714746590944827 6 26 2.892521898198318 6 27 2.640145232792541 6 28 3.228595390571229 6 29 2.670860890004228 6 30 3.373735733489667 6 31 3.141113685499441 6 32 3.1927890231950897 6 33 2.8888053084467273 6 34 2.3202820035632286 6 35 2.8255178580262097 6 36 2.896343248494908 6 37 3.6026361824123043 6 38 3.4778486310611854 6 39 4.480859943475657 6 40 7.734009269545719 6 41 4.787367353149868 6 42 5.915908086487313 6 43 7.361200022795259 6 44 6.630703745075447 6 45 6.411855125984189 6 46 5.3425229147377955 6 47 5.609038672758846 6 48 5.185409492726347 6 49 8.477048189696397 6 50 5.96583841771751 6 51 4.844871720248918 6 52 4.807220963167715 6 53 4.677010564719161 6 54 5.2974165594269955 6 55 5.2318428110362305 6 56 6.137511299189818 7 1 0.5093831581339785 7 2 0.989613270363968 7 3 0.5604619431558859 7 4 0.658348585036812 7 5 0.8153766081391893 7 6 0.7090384418835569 7 7 0.6983141381704172 7 8 1.2916732917255302 7 9 0.4825645072565976 7 10 -0.04803206354451106 7 11 1.0399003766969344 7 12 3.3027968199522135 7 13 3.2265555099188106 7 14 3.8991980702305113 7 15 4.536317051271773 7 16 4.420361039261273 7 17 4.225229040840873 7 18 5.384217420246038 7 19 3.330702263809094 7 20 3.6333160698989673 7 21 2.7936425766197175 7 22 3.073472469796876 7 23 2.7815121082020227 7 24 2.5361285563665206 7 25 2.562447740370118 7 26 1.972751783255788 7 27 1.4786509799189815 7 28 2.407216080226405 7 29 1.7823551428777904 7 30 1.927758721995417 7 31 1.6565159843500155 7 32 1.7602602875629003 7 33 0.9046673774122453 7 34 2.103615336896567 7 35 1.410805214348045 7 36 2.2269179611385823 7 37 2.5106821594238014 7 38 2.3171589758887734 7 39 3.081204771061863 7 40 6.30148053391353 7 41 -0.6834372445512784 7 42 2.8900460175217972 7 43 5.604188528542387 7 44 4.7386347795582076 7 45 4.357487309892235 7 46 5.2579252135883685 7 47 5.194670856666892 7 48 4.631386504220597 7 49 7.662795316133177 7 50 4.814459107372688 7 51 5.713377467375359 7 52 4.8133129171907 7 53 5.76379217391456 7 54 6.4084510421856145 7 55 5.453222121381053 7 56 5.0817641727530365 8 1 0.5975948662334218 8 2 1.4382900947424773 8 3 1.0867918014825335 8 4 0.8044325198136093 8 5 1.3453151272517232 8 6 1.3079424782374716 8 7 1.3616394522575632 8 8 1.3691926715704952 8 9 1.4941924142333392 8 10 0.8498562016252293 8 11 2.4456742339543567 8 12 4.666736942913985 8 13 4.854596140766176 8 14 4.7414862284769725 8 15 4.571735388614734 8 16 5.111620060913232 8 17 5.097856680509409 8 18 5.704719959673994 8 19 3.9286707214407457 8 20 3.689985409647697 8 21 3.4758131192553776 8 22 4.1147715876797974 8 23 3.6878205818721597 8 24 3.828296425920115 8 25 2.7670721723401783 8 26 2.79298701447739 8 27 2.722703372327423 8 28 2.2881302742921577 8 29 3.1778376341902757 8 30 3.2528055009315295 8 31 1.495997406429673 8 32 2.365579720869505 8 33 2.419502982865332 8 34 2.3516773524004435 8 35 1.2878434394215574 8 36 1.4461106903553684 8 37 1.7886826940402116 8 38 2.2634300264100204 8 39 1.8871390132431003 8 40 4.360288339313161 8 41 -1.4903070654547825 8 42 2.258931342301267 8 43 4.883525604190609 8 44 5.054192117168473 8 45 3.5055760562167464 8 46 4.16717407752849 8 47 4.7613642541541985 8 48 4.1668048415635575 8 49 7.075652840859185 8 50 4.776768650275653 8 51 4.887662417923337 8 52 4.6832674747956204 8 53 5.082824518207531 8 54 6.244858419892115 8 55 5.421145136617625 8 56 4.447976415468887 9 1 0.1700898418291672 9 2 0.37871501578755584 9 3 0.5676990653355389 9 4 0.35438945349147133 9 5 0.3023923595227558 9 6 0.7835382290270232 9 7 0.7511025587749316 9 8 0.7855004522193596 9 9 0.37145339614548334 9 10 0.647157378771368 9 11 1.5137190229293793 9 12 3.7446869859803087 9 13 3.412592121242781 9 14 3.585873251158567 9 15 3.996512879283692 9 16 4.009122214229343 9 17 4.302708819470077 9 18 4.685196560305634 9 19 3.4980075000798507 9 20 3.6486417403970464 9 21 2.4109265272370024 9 22 3.0649582084090525 9 23 2.2587364589895884 9 24 2.6566053550042383 9 25 2.1869688131670486 9 26 1.2720280710378233 9 27 2.095083504397479 9 28 0.5745213164971545 9 29 2.4251818776585523 9 30 1.9120073384279408 9 31 1.522842080561169 9 32 1.6534063071457084 9 33 0.7028793825208055 9 34 1.3381832381311298 9 35 0.9855178580262063 9 36 2.0183185571368796 9 37 2.071771984881437 9 38 2.1477251742710592 9 39 2.474563647179359 9 40 3.9600586522617682 9 41 2.9789722914214742 9 42 3.7214636420428704 9 43 5.000953109215011 9 44 4.464901275939642 9 45 3.263830434626165 9 46 3.667461186342731 9 47 3.3973102776971196 9 48 3.2891131964300477 9 49 5.4533444859926945 9 50 4.697813726359488 9 51 3.2063532017304013 9 52 2.6021592347726497 9 53 3.8702204412623686 9 54 4.600873349550451 9 55 4.495793428320177 9 56 4.575165620177472 10 1 0.5467056733469633 10 2 1.2622299032240107 10 3 1.1431529505933398 10 4 0.8716007824675032 10 5 1.123016905637499 10 6 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2.5900196436097858 114 25 2.112287574551388 114 26 2.186620258854056 114 27 1.3589976918089377 114 28 1.4682675217187722 114 29 1.3610248244304586 114 30 2.2196373728339296 114 31 0.9201300789420657 114 32 1.161805416637712 114 33 1.5960184232008245 114 34 0.9342164297927411 114 35 0.6576490055671869 114 36 1.5894580025932683 114 37 2.258373887330336 114 38 2.347684696634957 114 39 2.951187812328115 114 40 5.3587633679063735 114 41 6.133596861346589 114 42 2.4937769389463327 114 43 -0.5171606329424456 114 44 -5.3337224844327515 114 45 0.589068240738289 114 46 -1.4784606918195813 114 47 -1.442600671503449 114 48 -2.8244265728474254 114 49 -15.744591154565898 114 50 -1.452358303593961 114 51 -2.4718495912264906 114 52 -2.4126151024060576 114 53 -5.115612386100512 114 54 -9.555370325818906 114 55 -3.3127473528981994 114 56 -0.2321608319577244 115 1 -0.21115209662669798 115 2 0.34370488127493104 115 3 -0.04718945502562377 115 4 -0.05412353711107443 115 5 0.27051471072968525 115 6 0.24806743484975868 115 7 0.5329341416015367 115 8 0.505603639984237 115 9 0.3183854027789863 115 10 0.554472654245842 115 11 -0.5282931384857719 115 12 -10.607994053267895 115 13 -6.665535757880125 115 14 -7.7017626434425 115 15 -7.867868915446358 115 16 -8.192093927285306 115 17 -1.5819420005041245 115 18 -1.776015895931696 115 19 -1.1841373313187162 115 20 -1.643232223118705 115 21 2.2911550144306645 115 22 3.073987137835992 115 23 2.2568938203310296 115 24 2.887303715055836 115 25 2.815492859601541 115 26 2.1041636892430944 115 27 2.4752198596582176 115 28 2.2306849428100364 115 29 2.621607158660943 115 30 2.003586479758326 115 31 2.1029047302755615 115 32 1.3893561873741902 115 33 1.0168650099392664 115 34 1.979237227443825 115 35 1.5825327833993441 115 36 2.615149218644156 115 37 2.2295018540540923 115 38 2.2214307206134265 115 39 3.741009197206999 115 40 6.7514719561128835 115 41 6.453636009866287 115 42 1.956952862606716 115 43 -4.523277589145039 115 44 -6.638102225073808 115 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116 27 1.7337349763822871 116 28 1.751159493135333 116 29 1.3606044797478205 116 30 2.3583511181050554 116 31 0.9482931726789303 116 32 0.830994151400219 116 33 1.436497616139036 116 34 0.9754102086914358 116 35 1.5624409349492865 116 36 1.9532663254179852 116 37 0.5641746439507642 116 38 1.6942588874714417 116 39 2.4247060973218098 116 40 5.872214397750849 116 41 4.027880173662691 116 42 -1.3084508878716612 116 43 -8.918799977204742 116 44 -6.96339881902712 116 45 -1.1730166688876054 116 46 -5.527220675005797 116 47 -3.4896792759591015 116 48 -4.696641789324936 116 49 -8.18910565645745 116 50 -4.508264146385052 116 51 -1.7751282797510797 116 52 -1.7463687804220314 116 53 -3.031963794255198 116 54 -8.150788568778133 116 55 -3.3394392402458237 116 56 -1.5663348546563363 117 1 -0.6258433462608792 117 2 0.28575931498871654 117 3 0.21766275451491168 117 4 0.07552235109495342 117 5 0.06419337622573096 117 6 -0.07121848847479129 117 7 0.31900379334283 117 8 0.6053987819216076 117 9 0.5609958798056169 117 10 0.8316163475440632 117 11 -0.6997886023429558 117 12 -8.11370081358396 117 13 -7.647683840993967 117 14 -11.498367852690379 117 15 -8.27436584257542 117 16 -12.478895214964556 117 17 -10.27172836281024 117 18 -13.061353911735033 117 19 -4.545565484669606 117 20 -5.083384403393801 117 21 1.2642308119138406 117 22 1.6982189201011408 117 23 1.1527088627659126 117 24 1.6449858924043852 117 25 1.0039622772193368 117 26 2.432521898198317 117 27 2.9999491543611683 117 28 2.609379704296721 117 29 2.751253046866971 117 30 3.0325592629014366 117 31 1.735623489421009 117 32 1.2910243173127327 117 33 1.6484131515839877 117 34 1.8487133761122507 117 35 1.6325766815556193 117 36 2.082225601436079 117 37 2.406557751039756 117 38 2.271181964394522 117 39 3.4767422964168304 117 40 7.185185740133949 117 41 5.060308529620457 117 42 -0.1674252468460189 117 43 -8.771741153675329 117 44 -9.3796884117873 117 45 -1.1820664426432614 117 46 -4.917869242124951 117 47 -4.991941719398014 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2.813021447775384 118 31 1.4253993997851566 118 32 -2.225782405376343 118 33 0.9680910227324446 118 34 1.305996289277516 118 35 2.646232143740491 118 36 2.108486105637759 118 37 2.2454933252694502 118 38 1.723562916775471 118 39 1.5922885149042258 118 40 5.315437840974287 118 41 3.83236735314987 118 42 -1.6555204849412597 118 43 -9.6509428343476 118 44 -8.685010540638839 118 45 -1.3245734454443827 118 46 -7.758905656690779 118 47 -7.915961327241153 118 48 -8.736019078702228 118 49 -10.521523238875032 118 50 -5.676304439425344 118 51 -3.7215568511796526 118 52 -4.717064751118002 118 53 -4.655132292423698 118 54 -10.540440583430147 118 55 -2.4845857603923456 118 56 -2.108917272238756 119 1 -0.3882991482538074 119 2 0.22307850495978698 119 3 0.09937281558852362 119 4 -0.3966730844563102 119 5 0.07672953823151829 119 6 -0.026216881274535808 119 7 0.30807483159419746 119 8 0.3299246578457513 119 9 0.12190876955168051 119 10 0.176200082677461 119 11 -3.873044790063947 119 12 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-7.845800926544783 119 51 -6.258734837128127 119 52 -5.888024938471631 119 53 -7.67298943528084 119 54 -10.752091637294317 119 55 -6.14881292666869 119 56 -3.691177225400345 120 1 -1.4404601020541108 120 2 -0.8238663534604811 120 3 -0.9311786002087956 120 4 -1.8139607148586947 120 5 -0.9411542173571519 120 6 -1.0467086845532236 120 7 -1.0976628733238343 120 8 -0.6931751931229542 120 9 -1.3810718563797693 120 10 -0.7209683226876678 120 11 -2.6748331656228146 120 12 -11.018781027487705 120 13 -9.512211470227477 120 14 -12.469401720783072 120 15 -15.646023596586115 120 16 -18.096096640989515 120 17 -15.957111607017016 120 18 -14.838091879649472 120 19 -7.321857819785471 120 20 -13.853883512128203 120 21 -4.941205908228763 120 22 -3.614615304497793 120 23 -3.183743900157438 120 24 -8.794853679788126 120 25 -2.6716170454188095 120 26 0.6597946254710436 120 27 0.5355997782470894 120 28 1.3985953905712307 120 29 0.8617699809133228 120 30 0.8146448243987621 120 31 -0.4479772235914652 120 32 -7.388120067714002 120 33 -0.07574014609872393 120 34 -0.0024452691640419744 120 35 0.4627905852989329 120 36 1.1717977939494517 120 37 0.8980907278668511 120 38 1.483303176515733 120 39 0.7481326707483831 120 40 4.857645633182084 120 41 3.571003716786233 120 42 -0.4022737316945033 120 43 -11.350618159022925 120 44 -8.581114436742737 120 45 -3.53723578310672 120 46 -14.460204357989479 120 47 -13.722779509059334 120 48 -8.005499598182745 120 49 -16.57022453757633 120 50 -8.171434309555213 120 51 -6.297855552478353 120 52 -6.77550630955956 120 53 -8.241171253462657 120 54 -10.590765258754823 120 55 -6.447248098054683 120 56 -2.867034155355636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 85.0 5 25.0 6 26.0 7 44.0 8 106.0 9 212.0 10 115.0 11 131.0 12 98.0 13 96.0 14 94.0 15 137.0 16 174.0 17 230.0 18 316.0 19 423.0 20 463.0 21 640.0 22 777.0 23 925.0 24 1113.0 25 1270.0 26 1453.0 27 1534.0 28 1847.0 29 2247.0 30 2627.0 31 3183.0 32 3667.0 33 4242.0 34 4824.0 35 5908.0 36 7628.0 37 9396.0 38 8559.0 39 4435.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.3555487972658 0.30991585929240706 0.23895381674414562 0.09558152669765825 8 99.08763088152234 0.6241763334347077 0.2215753573445714 0.06661742769836787 9 96.7835368061288 1.9695587319517458 0.6907937611330756 0.5561107007863753 10 44.34548377286354 36.18629708476344 11.300343224573142 8.167875917799886 11 32.88438979884433 33.3999507610317 17.584104502469188 16.131554937654776 12 36.44842218070701 15.143879161778976 19.925851906561817 28.481846750952194 13 24.44129021044856 15.55407499674116 25.029329548252537 34.975305244557745 14 23.589387093036727 19.639960607113892 30.390453018190243 26.380199281659134 15 30.76308951822444 17.26930948890276 25.938076091216562 26.029524901656238 16 32.49423938089648 19.64842108314131 24.71921510658957 23.138124429372635 17 28.843897988443324 20.15322008370625 26.422499312102648 24.58038261574778 18 26.114031657760208 22.16043214435707 27.094466408886188 24.631069788996538 19 30.862211400495674 24.909778686029828 20.222618374711946 24.005391538762556 20 27.18179182371702 28.60829225862569 20.806030733545956 23.403885184111335 21 26.079274738961054 23.18576124893195 27.213219214783273 23.52174479732372 22 26.807721828793213 21.90120345831342 23.219069962781134 28.072004750112235 23 27.56947763247455 22.499312102648766 23.346511998378013 26.584698266498673 24 27.52208544532947 22.189717595944966 27.061549601737873 23.22664735698769 25 29.117425559031396 22.21642915073572 23.921040435638975 24.745104854593905 26 23.51620658691307 21.559540016800394 27.11091156620224 27.813341830084294 27 22.232531168454624 19.17947231081473 28.419831835314586 30.16816468541606 28 23.70298407911145 25.746019777861555 28.395151233941363 22.155844909085637 29 27.979312143250368 20.919653463912148 30.201663141424973 20.89937125141251 30 22.426928536253296 20.864111700124564 37.36710987514846 19.341849888473682 31 23.008388022078314 24.57878801048865 33.983803439233924 18.42902052819911 32 27.587906194741574 20.119140794851724 34.471113430153345 17.821839580253354 33 23.21664321258106 19.303921426395277 36.1763553801738 21.30307998084986 34 19.317770145922996 24.118593226970393 40.60484864293063 15.958787984175975 35 18.418535906798816 22.581580015069843 44.31548136555961 14.684402712571726 36 18.650132552984978 27.497138883657595 37.80440104883455 16.04832751452288 37 19.850839391169345 23.370913681278022 41.29920631465924 15.479040612893396 38 24.19111240197502 23.707522509439443 37.31774615161197 14.78361893697357 39 21.382652987186372 21.655468647966217 37.12469707303623 19.837181291811177 40 15.235428788077634 16.658646484973097 36.03686367149336 32.06906105545591 41 12.875423629776792 16.52594367184761 33.741381325230805 36.85725137314479 42 20.058890081517795 21.460067883148152 38.53964201792811 19.941400017405936 43 14.800563388462153 17.703175594244144 42.273011079005066 25.22324993828863 44 16.308288081061015 18.720610286945508 37.04668869833598 27.9244129336575 45 18.971322419598284 21.383373969580866 42.52583304307442 17.119470567746433 46 17.70766819527486 22.1164451152261 38.87502176815464 21.300864921344402 47 16.402990264255912 24.350950394065833 39.92379462216041 19.322264719517847 48 18.560485157995533 24.395728752575224 39.96750137828976 17.076284711139483 49 15.920905106513098 19.852352315913624 35.454373370997565 28.77236920657571 50 20.424353173221952 21.065378814247595 40.56735120083537 17.942916811695092 51 17.437789625067186 25.4129200017432 39.65484681648484 17.494443556704773 52 16.07627057202929 22.963822228666714 42.42151816138621 18.538389037917785 53 16.869550077622854 22.750025390653338 43.14960172946621 17.23082280225759 54 15.974208913608573 24.456513846734726 37.019503056882705 22.549774182773994 55 16.280960538923892 26.449664624408374 38.42300879816487 18.84636603850286 56 20.402536531568792 23.846008735652198 37.408036219581206 18.3434185131978 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 3.0 11 5.0 12 4.5 13 41.5 14 79.0 15 139.0 16 199.0 17 226.0 18 253.0 19 286.5 20 320.0 21 378.0 22 548.0 23 660.0 24 733.5 25 807.0 26 981.5 27 1156.0 28 1381.5 29 1978.0 30 2349.0 31 2489.0 32 2629.0 33 2838.5 34 3048.0 35 3082.0 36 3116.0 37 2794.5 38 2130.5 39 1788.0 40 1566.5 41 1345.0 42 1276.0 43 1207.0 44 1164.0 45 1121.0 46 1147.5 47 1240.0 48 1306.0 49 1883.5 50 2461.0 51 2392.0 52 2323.0 53 2091.0 54 1909.5 55 1960.0 56 2252.0 57 2544.0 58 2697.0 59 2850.0 60 2990.0 61 3130.0 62 3310.0 63 3847.5 64 4205.0 65 4301.0 66 4397.0 67 4027.0 68 3657.0 69 3228.5 70 2800.0 71 2447.5 72 1797.0 73 1499.0 74 1293.5 75 1088.0 76 905.5 77 723.0 78 578.0 79 327.0 80 221.0 81 178.5 82 136.0 83 96.0 84 56.0 85 40.5 86 25.0 87 18.0 88 6.0 89 1.0 90 2.0 91 3.0 92 1.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.011585639599716152 14 0.004344614849893557 15 0.23171279199432304 16 0.06951383759829691 17 0.0 18 0.0 19 0.07820306729808403 20 0.10282255144748084 21 0.0 22 0.0 23 0.0 24 0.001448204949964519 25 0.004344614849893557 26 0.007241024749822595 27 0.10282255144748084 28 0.12309742074698411 29 0.03620512374911297 30 0.01303384454968067 31 0.034756918799148454 32 0.08254768214797759 33 0.17668100389567132 34 0.0608246078985098 35 0.056479993048616235 36 0.03186050889921942 37 0.1911630533953165 38 0.2766071454432231 39 0.20274869299503265 40 0.686449146283182 41 0.8616819452288889 42 0.15785433954613257 43 0.2635733008935424 44 0.5242501918871558 45 0.21288612764478432 46 0.20709330784492624 47 0.039101533649042015 48 0.1810256187455649 49 0.5416286512867301 50 0.14337229004648738 51 0.307019449392478 52 0.12454562569694863 53 0.18537023359545843 54 0.2751589404932586 55 0.2505394563438618 56 0.1998522830951036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 69051.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.31247918205385 #Duplication Level Percentage of deduplicated Percentage of total 1 86.73132765738893 63.58488653314217 2 7.769195820081781 11.391580136420906 3 2.409971751970448 5.30043011687014 4 1.0963396084783597 3.2150149889212325 5 0.624222191493985 2.28816382094394 6 0.33384034924836536 1.4684798192640223 7 0.2607510420164747 1.3381413737672156 8 0.16000632123738223 0.9384368075770083 9 0.10667088082492147 0.7038276056827563 >10 0.46816664362048876 5.685652633560701 >50 0.025680026865258873 1.071671662973744 >100 0.011852320091657943 1.6958479964084516 >500 0.0019753866819429903 1.3178665044677123 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 910 1.3178665044677123 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 251 0.3634994424410942 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 240 0.3475691879914845 No Hit GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 195 0.2823999652430812 No Hit GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 173 0.2505394563438618 No Hit GCGTCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 164 0.23750561179418112 No Hit GCGTCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 148 0.2143343325947488 No Hit GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 73 0.10571896134740988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.001448204949964519 0.0 0.0 0.0 0.0 11 0.004344614849893557 0.0 0.0 0.0 0.0 12 0.005792819799858076 0.0 0.0 0.0 0.0 13 0.005792819799858076 0.0 0.0 0.0 0.0 14 0.007241024749822595 0.0 0.0 0.0 0.0 15 0.007241024749822595 0.0 0.0 0.0 0.0 16 0.008689229699787113 0.0 0.0 0.0 0.0 17 0.011585639599716152 0.0 0.0 0.0 0.0 18 0.01448204949964519 0.0 0.0 0.0 0.0 19 0.018826664349538745 0.0 0.0 0.0 0.0 20 0.027515894049325862 0.0 0.0 0.0 0.0 21 0.02896409899929038 0.0 0.0 0.0 0.0 22 0.03186050889921942 0.0 0.0 0.0 0.0 23 0.03620512374911297 0.0 0.0 0.0 0.0 24 0.04199794354897105 0.0 0.0 0.0 0.0 25 0.047790763348829125 0.0 0.0 0.0 0.0 26 0.055031788098651724 0.0 0.0 0.0 0.0 27 0.0608246078985098 0.0 0.0 0.0 0.0 28 0.06951383759829691 0.0 0.0 0.0 0.0 29 0.07241024749822594 0.0 0.0 0.0 0.0 30 0.07530665739815498 0.0 0.0 0.0 0.0 31 0.07965127224804855 0.0 0.0 0.0 0.0 32 0.08109947719801307 0.0 0.0 0.0 0.0 33 0.08254768214797759 0.0 0.0 0.0 0.0 34 0.08544409204790662 0.0 0.0 0.0 0.0 35 0.0912369118477647 0.0 0.0 0.0 0.0 36 0.09268511679772921 0.0 0.0 0.0 0.0 37 0.09558152669765825 0.0 0.0 0.0 0.0 38 0.09847793659758729 0.0 0.0 0.0 0.0 39 0.0999261415475518 0.0 0.0 0.0 0.0 40 0.0999261415475518 0.0 0.0 0.0 0.0 41 0.10137434649751632 0.0 0.0 0.0 0.0 42 0.10282255144748084 0.0 0.0 0.0 0.0 43 0.10861537124733893 0.0 0.0 0.0 0.0 44 0.11730460094712604 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTGTC 30 9.811174E-7 50.37729 38 TGCCCGT 15 0.008175101 50.340412 37 ACTGTGC 15 0.008175101 50.340412 41 TATGCTT 205 0.0 50.266815 35 CGATTCA 25 2.0027068E-5 50.26681 49 CGATTAA 40 2.4992914E-9 50.26681 49 TATGCCT 30 9.961168E-7 50.26681 35 TATGCCC 15 0.008222564 50.26681 35 ACGATTC 25 2.0113976E-5 50.230095 48 ACGATTA 40 2.5156623E-9 50.230095 48 AACGATT 60 0.0 50.230095 47 AAGATTA 240 0.0 50.230095 48 ATATGCC 55 0.0 50.156822 34 ATATGCT 280 0.0 50.15682 34 AATGAGG 15 0.008318105 50.120262 43 ATGAGGT 15 0.008318105 50.120262 44 ATAAACA 15 0.008318105 50.120262 50 GATTCAG 25 2.037658E-5 50.120262 50 TAATGAG 15 0.008342118 50.08376 42 GGATAAG 15 0.0083903 50.01091 32 >>END_MODULE