FastQCFastQC Report
Fri 17 Jun 2016
SRR1526210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39100
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG700.17902813299232737No Hit
GCCTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.17902813299232737No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT550.14066496163682865No Hit
GCCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.11508951406649616No Hit
GCCTAGGGGGTATTCATGGTGGGGAAAAAGTTTCCACTTTGAAAAGTCTGGTATAT430.10997442455242966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTT251.977384E-550.3058135
ATGCTTG251.977384E-550.3058136
ATATGCT309.972155E-750.1763234
CCAAAAC150.00829670650.11182843
AACGATT150.00838148549.98333747
ATAGCCT150.00838148549.98333715
CCCGAGT150.00838148549.98333713
ACGATTA150.00838148549.98333748
CGGTTGA204.1407885E-449.98333714
CATATGC301.0241747E-649.98333733
CCGGTTG204.1407885E-449.98333713
CAACGAT150.00838148549.98333746
CGATTAA150.00838148549.98333749
AGCCTCT204.1671132E-449.91933417
ATCCCAG204.1671132E-449.91933426
TCCTCGT150.00842411549.91933427
GAGTGTC150.00842411549.9193349
GACAGTT150.00842411549.9193349
GACAGTG252.0699792E-549.9193349
CCCGGTT204.1671132E-449.91933412
Produced by FastQC (version 0.11.5)