FastQCFastQC Report
Fri 17 Jun 2016
SRR1526209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77011
Sequences flagged as poor quality0
Sequence length56
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2690.34930074924361454No Hit
TTCATAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1140.14803080079469166No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.13115009544091102No Hit
TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG850.11037384269779642No Hit
TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC820.10647829530846242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACA309.771393E-750.41814839
CGCCAAA150.008128921550.41814838
TACGCCC203.9916649E-450.41814438
CGTCTCA150.00815008750.3850241
GTCTTGC204.0046475E-450.3850241
GTACGCC204.043797E-450.28590437
TCTCGTT150.0082565350.2200444
CCAACAC204.0700653E-450.2200443
ACCAACA150.00827794250.18717642
CAACGAT252.0395726E-550.1215746
CGTGCAA150.0083208950.1215748
ATCAGGC150.0083208950.1215749
ATATGCC252.0395726E-550.1215734
CGATTAA150.0083208950.1215749
AAGATTA550.050.1215748
AGTTCTG850.050.12156745
GGTACCG150.00836400450.05613715
AGGAATC150.00836400450.05613715
TAGCCAG150.00836400450.05613750
TGATTGT150.00836400450.05613715
Produced by FastQC (version 0.11.5)