##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526209_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77011 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.6626715663996 39.0 38.0 40.0 33.0 40.0 2 37.49573437560868 39.0 38.0 40.0 33.0 40.0 3 37.26017062497565 39.0 38.0 40.0 33.0 40.0 4 35.79499032605732 39.0 37.0 40.0 24.0 40.0 5 37.23883601044007 39.0 38.0 40.0 33.0 40.0 6 34.272545480515774 39.0 35.0 40.0 16.0 40.0 7 37.0619781589643 39.0 37.0 40.0 32.0 40.0 8 37.11538611367207 39.0 37.0 40.0 32.0 40.0 9 37.09435015776967 39.0 38.0 40.0 32.0 40.0 10 36.587292724415995 39.0 36.0 40.0 31.0 40.0 11 35.86517510485515 38.0 35.0 40.0 29.0 40.0 12 33.912999441638206 38.0 32.0 39.0 21.0 40.0 13 33.616210671202815 38.0 31.0 39.0 20.0 40.0 14 33.743608055992 38.0 32.0 39.0 20.0 40.0 15 33.73236290919479 38.0 32.0 39.0 20.0 40.0 16 33.81229954162392 38.0 32.0 39.0 21.0 40.0 17 33.844048252846996 38.0 32.0 39.0 22.0 40.0 18 33.26668917427381 38.0 31.0 39.0 19.0 40.0 19 34.28215449740946 38.0 32.0 39.0 24.0 40.0 20 34.34732700523302 38.0 32.0 39.0 25.0 40.0 21 35.161756112763115 38.0 35.0 39.0 26.0 40.0 22 34.864045396112246 38.0 34.0 39.0 25.0 40.0 23 35.365441300593425 38.0 35.0 39.0 27.0 40.0 24 34.938710054407814 38.0 34.0 39.0 25.0 40.0 25 35.23596629052992 38.0 34.0 39.0 27.0 40.0 26 35.88631494202127 38.0 35.0 39.0 29.0 40.0 27 35.78935476750075 38.0 35.0 39.0 29.0 40.0 28 35.78682266169768 38.0 35.0 39.0 29.0 40.0 29 35.578748490475384 38.0 35.0 39.0 28.0 40.0 30 35.543662593655455 38.0 35.0 39.0 28.0 40.0 31 35.93052940489021 38.0 35.0 39.0 30.0 40.0 32 35.59505784887873 38.0 35.0 39.0 29.0 40.0 33 35.76839672254613 38.0 35.0 39.0 29.0 40.0 34 35.75600888184805 38.0 35.0 39.0 29.0 40.0 35 35.67994182649232 38.0 35.0 39.0 29.0 40.0 36 35.03930607315838 38.0 35.0 39.0 29.0 40.0 37 34.83445222111127 38.0 34.0 39.0 29.0 40.0 38 34.53011907389853 38.0 34.0 39.0 28.0 40.0 39 34.01223201880251 38.0 33.0 39.0 25.0 40.0 40 30.69731596784875 35.0 27.0 39.0 10.0 40.0 41 27.274791912843618 32.0 16.0 38.0 6.0 39.0 42 31.617080676786433 35.0 30.0 38.0 16.0 40.0 43 30.04148757969641 35.0 27.0 38.0 7.0 40.0 44 29.92994507278181 35.0 27.0 38.0 6.0 40.0 45 31.231499396190156 35.0 30.0 38.0 10.0 40.0 46 30.825466491799872 35.0 29.0 38.0 4.0 40.0 47 30.34286011089325 35.0 28.0 38.0 4.0 40.0 48 30.474724390022203 35.0 28.0 38.0 4.0 40.0 49 27.704600641466804 34.0 17.0 38.0 4.0 40.0 50 30.055355728402436 35.0 27.0 38.0 4.0 40.0 51 30.310656919141422 36.0 29.0 39.0 4.0 40.0 52 30.10849099479295 35.0 28.0 39.0 4.0 40.0 53 29.72322135798782 35.0 27.0 39.0 4.0 40.0 54 28.637584241212295 35.0 22.0 39.0 4.0 40.0 55 29.087377127942762 35.0 26.0 39.0 4.0 40.0 56 27.818480476815 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2189156824929057 1 2 1.4821933482058682 1 3 1.408917569741547 1 4 3.687216800950111 1 5 1.6437293042789705 1 6 5.485299942955507 1 7 1.9003811386128007 1 8 1.7618915219198996 1 9 1.7632367478772153 1 10 1.8172419579902694 1 11 -1.0355755660984087 1 12 -11.275442433707621 1 13 -6.542637673029908 1 14 -4.689215263870551 1 15 -5.042357785187775 1 16 -23.13670146750958 1 17 -4.534263623450869 1 18 -13.605376290209765 1 19 -5.100851095468766 1 20 -5.179262251173981 1 21 -5.967485285475174 1 22 -3.01698455804474 1 23 -3.9812671558131463 1 24 -4.15102567306155 1 25 -3.4993329380424285 1 26 1.8819732599558563 1 27 2.207040639258331 1 28 1.8371066521900303 1 29 2.226046222041461 1 30 2.467538542501039 1 31 1.934136174650412 1 32 2.1807107114353173 1 33 2.0328392750741813 1 34 1.4108784651166317 1 35 1.819300636866494 1 36 1.9315479883970639 1 37 1.8634330671785122 1 38 1.4561472103551552 1 39 2.8671467751034783 1 40 6.06640215615467 1 41 -8.106752352221054 1 42 -7.94226485100938 1 43 -15.674482037356187 1 44 -22.06046254109006 1 45 -9.814520426542732 1 46 -15.909940573065441 1 47 -23.56259373693544 1 48 -13.992619492556416 1 49 -19.793438692182363 1 50 -12.880088835981333 1 51 -9.097689935991351 1 52 -8.787610508668262 1 53 -8.878696252572759 1 54 -8.031330055314097 1 55 -8.238989620771779 1 56 -3.401377042836401 2 1 1.9269455699420632 2 2 2.2097068259321517 2 3 2.4296307069779033 2 4 4.28506036474468 2 5 2.505263400561951 2 6 6.102627097027202 2 7 2.569349264972395 2 8 2.2507783090191182 2 9 2.2555284342481556 2 10 2.775626522446906 2 11 2.568983215704293 2 12 -0.4374588907207695 2 13 0.35928231183720527 2 14 4.155010594447809 2 15 3.709652051187895 2 16 2.4390102510187432 2 17 3.4020836762748488 2 18 -1.2855995002731362 2 19 2.2896719348915866 2 20 2.3258923880012787 2 21 2.3565476285511195 2 22 2.4831573127582516 2 23 2.326876228278408 2 24 1.313553916458929 2 25 2.1682164802872776 2 26 2.501475766340043 2 27 2.1100955905493564 2 28 2.173151294202704 2 29 2.8111214135670437 2 30 2.571198809218153 2 31 2.1100559703564556 2 32 2.623725938901508 2 33 2.3440092362961593 2 34 1.7098475372815827 2 35 1.9076388568608138 2 36 2.2061152824479464 2 37 2.20988156807703 2 38 2.6342423583736903 2 39 3.049498047211678 2 40 6.182736214605438 2 41 -5.774680092692066 2 42 -5.124805284188234 2 43 -12.130454798162013 2 44 -12.255487604931922 2 45 -4.023827151218793 2 46 -8.211463319684185 2 47 -17.627948886845587 2 48 -6.317692792471295 2 49 -11.249884355664818 2 50 -5.512927197846459 2 51 -0.06534339290991653 2 52 -0.24150249769692067 2 53 -0.08421975583578956 2 54 1.0104745412999208 2 55 0.30882083983542685 2 56 4.211031923398348 3 1 1.8653975161431902 3 2 2.197236887391135 3 3 2.182013355949131 3 4 3.94044470606471 3 5 2.502502199885015 3 6 5.981846835158493 3 7 2.569883690909869 3 8 2.3263995791714294 3 9 2.213041572219119 3 10 2.3874551498629515 3 11 2.7794579640787447 3 12 4.993199343892215 3 13 3.7209996005162864 3 14 4.650690651453239 3 15 4.273026060273139 3 16 3.891223665109777 3 17 3.96118227786031 3 18 4.491010457863503 3 19 3.984425653605399 3 20 3.714241902564389 3 21 2.6408622272863127 3 22 3.5097895386422806 3 23 2.79343007169161 3 24 2.9977081874128757 3 25 2.8150500066077555 3 26 2.49132167352807 3 27 2.1217638901841624 3 28 2.1556933802452747 3 29 2.555042318528301 3 30 1.9689898486765998 3 31 1.0757636393686596 3 32 1.3957042055800457 3 33 1.1877787205751318 3 34 0.5739252071844945 3 35 1.0213825105528116 3 36 2.392362721656994 3 37 2.4884956234791815 3 38 1.9630033809086527 3 39 2.614119050151018 3 40 7.050287653101922 3 41 7.25827617345205 3 42 3.2524103566775366 3 43 -0.6875938379767454 3 44 0.5450824494013808 3 45 3.429098830788867 3 46 2.270321662946973 3 47 -2.6894078696548895 3 48 2.8111038584595 3 49 4.664073068402164 3 50 2.2073898948764388 3 51 3.676707021270186 3 52 3.3557184874282235 3 53 4.056245194729815 3 54 4.786906357457401 3 55 4.303298438481551 3 56 5.616839351918877 4 1 2.3028351352340124 4 2 2.4642725465872175 4 3 2.6461273201991986 4 4 4.386320760869175 4 5 2.610296410936641 4 6 6.288171125150328 4 7 2.859240373606532 4 8 2.723641208787612 4 9 2.746568475403947 4 10 3.0685185742346093 4 11 3.2564564215689913 4 12 5.591693128485268 4 13 5.506500008827693 4 14 5.725275534857651 4 15 5.008803212981604 4 16 4.898754742144526 4 17 5.297993819462711 4 18 5.932440455141425 4 19 4.578019701332465 4 20 4.39131294874894 4 21 3.690766954625026 4 22 4.120986344739727 4 23 3.7102252808377827 4 24 4.268282545456614 4 25 3.6592565214430692 4 26 3.3954138611698497 4 27 2.9020016181580317 4 28 3.226739564802031 4 29 3.5544979033209714 4 30 3.0757859651814528 4 31 2.307775253559747 4 32 2.8433949777422853 4 33 2.2159975754884798 4 34 2.289286789618032 4 35 2.3958494373289625 4 36 3.2378395386427528 4 37 3.302396358439715 4 38 3.30671084847058 4 39 4.529734693014639 4 40 8.25861720577409 4 41 10.429041076818354 4 42 6.728319983753117 4 43 8.23283080588497 4 44 7.431299671069105 4 45 6.803747229300804 4 46 7.120698216798711 4 47 6.4925175454617055 4 48 6.387185883684072 4 49 8.806619116855117 4 50 6.141878598260888 4 51 5.574629169895534 4 52 5.959474952358814 4 53 5.590135041386194 4 54 7.178613099132381 4 55 5.472157888622188 4 56 6.559312811677522 5 1 1.350187160156132 5 2 2.157107437552952 5 3 2.0899190424955947 5 4 3.9676291720841306 5 5 2.3383782978970302 5 6 6.071259687678136 5 7 2.436365429236261 5 8 2.147851266642533 5 9 2.3229814705085303 5 10 2.677330323233271 5 11 2.7701190828952065 5 12 5.431479510327726 5 13 5.430754570465432 5 14 5.241074377759297 5 15 5.328874419034122 5 16 4.833156166265574 5 17 4.942319587152959 5 18 4.482596218381296 5 19 3.944018811256811 5 20 4.0497019118108 5 21 3.2472422550255615 5 22 3.2783517207966923 5 23 3.1081781068279923 5 24 3.3641436936542064 5 25 3.4261457070238492 5 26 2.1710257881836412 5 27 2.638361208723225 5 28 2.482369293328958 5 29 2.47857444982742 5 30 2.085309774705266 5 31 1.7320743189803096 5 32 2.176194265681758 5 33 1.7973949942690766 5 34 2.300323727757771 5 35 2.5407537541958973 5 36 3.185058052216405 5 37 3.712721236054307 5 38 3.8428924239044875 5 39 3.9425517824672482 5 40 8.146912710892028 5 41 10.59290400516726 5 42 6.71870716666367 5 43 8.133765385324228 5 44 8.191869319489221 5 45 6.7580863481259 5 46 5.9702754308619 5 47 6.750906508523567 5 48 6.376059937978685 5 49 8.608132245471047 5 50 6.154962710410423 5 51 5.305648306522148 5 52 5.520489638607142 5 53 5.213989068978538 5 54 5.838871221072747 5 55 5.841717301172256 5 56 6.206575829033994 6 1 2.179598924862013 6 2 2.443381947356869 6 3 2.4941207231678675 6 4 4.2499821132606 6 5 2.6408993063003976 6 6 6.205433357145928 6 7 2.6091945608889233 6 8 2.501632779247572 6 9 2.484886232413295 6 10 2.9784507714125468 6 11 3.446029447040864 6 12 5.53231984646218 6 13 5.443919836571872 6 14 5.694855890364337 6 15 6.018790385420672 6 16 5.0191505640246845 6 17 5.441479251018507 6 18 5.8041648458322825 6 19 4.647380155794622 6 20 4.482755133099317 6 21 3.1816960365381632 6 22 4.006362925278488 6 23 3.5922117202733688 6 24 3.4316506964553284 6 25 3.525025258844572 6 26 2.783630830200451 6 27 2.360770172308662 6 28 2.5708846994914225 6 29 2.43263607447728 6 30 2.624805573024588 6 31 2.228152750352855 6 32 2.391040204057113 6 33 2.5455742659777556 6 34 1.7980828313992276 6 35 2.347196331226712 6 36 2.9010244387710244 6 37 2.613281460143945 6 38 3.108158530347069 6 39 3.9744845555764883 6 40 6.917220834141329 6 41 4.032119691441771 6 42 5.125149743694479 6 43 6.8945496990497155 6 44 6.266099011365974 6 45 5.2499630988261785 6 46 5.63554153730448 6 47 6.495724435694438 6 48 6.9418862685108955 6 49 8.384042609616706 6 50 6.896139180998652 6 51 6.764751947978734 6 52 6.604243140007707 6 53 6.759367220239046 6 54 7.446434246282831 6 55 5.717347553273097 6 56 5.1368279298743325 7 1 2.0083431885249254 7 2 2.3032067283330875 7 3 2.219402325170371 7 4 4.101671725275615 7 5 2.2902526646650188 7 6 6.101553507333662 7 7 2.5495366551734477 7 8 2.388783384170395 7 9 2.4529713387962744 7 10 2.1484549432940625 7 11 2.97001776577261 7 12 5.524184677500976 7 13 5.057603607952764 7 14 4.55009160167014 7 15 5.332724469692678 7 16 4.672467209831943 7 17 4.9283378242350295 7 18 5.432241608452223 7 19 3.841789834559748 7 20 4.017381749703404 7 21 3.199217938916135 7 22 3.486963676217158 7 23 3.232595533373079 7 24 2.6823807757210787 7 25 3.355021086963056 7 26 2.803447267413631 7 27 2.551633751783008 7 28 2.093412859691675 7 29 2.8064366585406972 7 30 2.937488092841029 7 31 2.1810104891930706 7 32 2.360794063047514 7 33 1.9479522384433423 7 34 1.986443281962437 7 35 2.3841174826660065 7 36 2.562059065387629 7 37 2.4305530496308023 7 38 2.2103696275519056 7 39 3.154709837178494 7 40 6.474167016869739 7 41 -1.5886562785206841 7 42 2.9670354341408682 7 43 4.901850491707204 7 44 4.873733554544948 7 45 3.466900937791557 7 46 5.049809372097972 7 47 5.2036927293948985 7 48 4.694702288535925 7 49 7.2165415666463275 7 50 5.082622284878504 7 51 4.898252156572806 7 52 4.367906051981006 7 53 5.114394337468919 7 54 6.121444675986627 7 55 4.719224899956448 7 56 5.66311078344129 8 1 1.7148130104962718 8 2 2.1720734239475092 8 3 2.367470062903763 8 4 3.959465906778007 8 5 2.0594667332711865 8 6 5.90051138835841 8 7 2.243848422433544 8 8 2.085266232628925 8 9 2.2910086813928885 8 10 2.534473180376132 8 11 2.8810034366367034 8 12 4.880459102164458 8 13 4.697421237132097 8 14 4.77848934374569 8 15 4.623432242163368 8 16 4.4345167104832655 8 17 4.634156321846838 8 18 4.834977170762251 8 19 3.3222501037738184 8 20 3.76466789820536 8 21 3.2513238876786232 8 22 3.2776714486878475 8 23 2.817701916351801 8 24 3.27638859161339 8 25 2.397574278452417 8 26 1.8696652439659545 8 27 0.8145516849137024 8 28 1.2143420824446025 8 29 2.2955812525485086 8 30 1.6778267815079815 8 31 1.2667681965313307 8 32 1.3714323609198544 8 33 1.4273269670581925 8 34 0.8833169250366879 8 35 0.8189850467129034 8 36 0.8149900250055211 8 37 0.801156610203968 8 38 0.6775863014555128 8 39 1.8466334151203085 8 40 4.1333072687151535 8 41 -5.347232049254508 8 42 2.600339819724894 8 43 4.903153140426266 8 44 5.256495169829357 8 45 2.200943490983043 8 46 3.5485067233789103 8 47 3.539341882673231 8 48 2.767216400563715 8 49 6.298608435947237 8 50 2.2814933226553187 8 51 3.5532673541411945 8 52 4.281714128403063 8 53 3.9194312458492924 8 54 4.50485761563057 8 55 4.21314587260083 8 56 4.629024808625832 9 1 1.8277233920401912 9 2 2.0795712056688913 9 3 2.0970618996384545 9 4 4.150237867736301 9 5 2.1251277802986905 9 6 5.926021592440044 9 7 2.494957561741437 9 8 2.2569609974913902 9 9 2.3979297106741626 9 10 2.5950321865873107 9 11 3.300036267160216 9 12 5.698560255669342 9 13 5.3961790011072495 9 14 5.841488456434249 9 15 5.161586634355032 9 16 4.853549541006778 9 17 5.340456233115695 9 18 5.6470889720044894 9 19 4.091327299869647 9 20 4.217196735827365 9 21 2.7584223792491613 9 22 3.7288693191403794 9 23 3.535093220699629 9 24 3.73557226508278 9 25 3.0388786262785032 9 26 1.991005084249899 9 27 2.167036156963391 9 28 1.860526643225434 9 29 2.410044429123488 9 30 2.391003356485804 9 31 1.8581612755020487 9 32 1.8714323609198544 9 33 2.149154828637606 9 34 1.4054997111946292 9 35 1.5927206279018975 9 36 1.3132154021128812 9 37 1.9668620228037952 9 38 1.6897868338423763 9 39 3.335098366318178 9 40 5.8839727522998935 9 41 3.3805851645875507 9 42 3.961253750514601 9 43 5.520721907063358 9 44 5.338349650752164 9 45 5.005291317069997 9 46 5.235285782828779 9 47 4.55753176732274 9 48 5.406080641912428 9 49 7.694571168866496 9 50 4.922354014758248 9 51 4.348298410041817 9 52 4.364899576495343 9 53 4.5008420710489325 9 54 6.091148653784966 9 55 3.8855061210480315 9 56 5.023656574375611 10 1 1.725944735913707 10 2 2.0677134981590086 10 3 2.187970990547548 10 4 4.288083717538676 10 5 2.2269064364251747 10 6 5.732839774258224 10 7 2.4010840439548815 10 8 2.452613171404437 10 9 2.3257953233223887 10 10 2.9809017518047014 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16 -12.43268234305119 118 17 -10.84070318717416 118 18 -12.726099433792612 118 19 -4.665919076941947 118 20 -5.529180075766838 118 21 0.33088614736510635 118 22 1.496985261169364 118 23 1.1582816264967306 118 24 0.9094853085610382 118 25 0.977284423379956 118 26 1.7192659538151105 118 27 1.8010941279778834 118 28 1.3713962084428317 118 29 1.5912038494133398 118 30 1.5105685738771015 118 31 0.30019026100929125 118 32 -4.418422711543915 118 33 0.33031424892745775 118 34 0.47796347931056715 118 35 -0.1536561836923056 118 36 1.001621199214334 118 37 1.0212098488907486 118 38 1.200656399059767 118 39 1.7843737286370143 118 40 4.941943766792651 118 41 2.2972518312542185 118 42 -3.114833206007141 118 43 -9.45753896250186 118 44 -10.766722813015953 118 45 -5.5599260742343475 118 46 -13.373409869345135 118 47 -13.71783055151784 118 48 -12.586672981275974 118 49 -14.903254918090028 118 50 -10.842138738864943 118 51 -9.825614633436441 118 52 -9.819883032200309 118 53 -10.415824595617732 118 54 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18 -15.265551929766847 120 19 -8.04434097710298 120 20 -15.266700204591313 120 21 -5.561223353440049 120 22 -5.0110662686212954 120 23 -4.071186972537088 120 24 -10.319983290472777 120 25 -3.2320553511772125 120 26 -0.176064158906307 120 27 -0.25687688651486695 120 28 -0.2678952569355957 120 29 -0.4595207882678167 120 30 -0.4056955163000282 120 31 -1.893851606945617 120 32 -12.684123194635703 120 33 -1.8444039475298695 120 34 -2.142004314570272 120 35 -2.1190346055924607 120 36 -0.7133546461962936 120 37 -0.35077082743775634 120 38 0.21273369374576134 120 39 0.39387453378999737 120 40 2.8823624463417623 120 41 -0.5996885874253337 120 42 -5.396636748680248 120 43 -12.121790170231332 120 44 -11.387495759875858 120 45 -8.595352805313254 120 46 -18.000624040037568 120 47 -17.591421533804475 120 48 -14.824998263079516 120 49 -18.53207939474059 120 50 -15.15614840070069 120 51 -12.952023651149808 120 52 -13.6234257053082 120 53 -14.273312518323046 120 54 -17.05901237681085 120 55 -13.226007566552612 120 56 -10.551625229167062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 620.0 5 56.0 6 52.0 7 65.0 8 64.0 9 61.0 10 56.0 11 39.0 12 53.0 13 72.0 14 102.0 15 161.0 16 219.0 17 269.0 18 334.0 19 439.0 20 553.0 21 626.0 22 886.0 23 1044.0 24 1182.0 25 1368.0 26 1599.0 27 1598.0 28 1944.0 29 2318.0 30 2795.0 31 3255.0 32 3734.0 33 4217.0 34 4834.0 35 5864.0 36 7350.0 37 10052.0 38 11213.0 39 7905.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.58447494513771 0.3103452753502746 0.06882133721156718 0.036358442300450586 8 99.47929516562569 0.41162950747295846 0.06492578982223318 0.04414953707911857 9 97.301684174988 1.672488345820727 0.5479736660996481 0.47785381309163627 10 38.720442534183434 43.51196582306424 9.223357702146446 8.544233940605887 11 29.06467907182091 33.36536338964564 21.718975211333447 15.850982327200011 12 36.625936554518184 15.914609601225798 22.13320175039929 25.32625209385672 13 23.683356062081952 17.90375998441457 26.5796480290928 31.833235924410673 14 25.03960524607194 21.806258927412024 27.274379950655756 25.879755875860276 15 26.71380024217804 20.303894379125815 27.649961590042054 25.33234378865409 16 27.59781182187918 22.54187294533453 27.515950051326033 22.344365181460258 17 27.908063887806776 23.180106479677963 24.705882352941178 24.20594727957408 18 24.07089988313206 24.803272302298403 25.612258148292426 25.513569666277107 19 23.96579285964935 27.508675253109445 23.857920798513184 24.66761108872802 20 24.568130711138263 26.44898808053761 24.005303315872254 24.977577892451876 21 24.658814974484162 23.14344704003324 27.866148991702484 24.33158899378011 22 24.741919985456622 23.590136474010208 25.262624819830933 26.405318720702237 23 24.382231109841452 26.158600719377752 26.340392930880004 23.118775239900792 24 23.352508083260833 25.47038734726208 27.344145641531732 23.83295892794536 25 24.39163744968186 26.935462926892612 26.97831450460979 21.694585118815738 26 24.235104669887278 24.174068879538726 30.021817048465017 21.569009402108982 27 23.351355566530632 22.975747966000366 30.96618231914949 22.706714148319513 28 22.7779150037051 23.45653332639981 33.198996372902066 20.56655529699302 29 22.97111238115031 22.59183249337559 34.82101106665974 19.61604405881436 30 20.809090909090912 20.771428571428572 39.24415584415584 19.175324675324674 31 19.22792159715277 22.128411290217826 39.98207489576162 18.661592216867785 32 21.90367270553375 20.494892522028437 40.501130663062405 17.100304109375404 33 19.457413741530218 20.795671795138574 42.6929029405262 17.05401152280501 34 16.827541409548555 21.616109126339722 45.68626177330302 15.870087690808704 35 18.549937642901686 20.467418416129703 46.735346081895656 14.247297859072958 36 17.396048478235453 21.972383513243184 45.13983606770326 15.491731940818102 37 17.82392048189636 20.662737598064087 46.611503584299335 14.901838335740214 38 19.15780153387414 21.38829932681415 45.194598888006354 14.259300251305357 39 17.4874123417598 20.989838799911528 44.84979378358335 16.672955074745317 40 13.606411630886697 15.929712627147453 40.29234108202808 30.171534659937766 41 12.298645852431966 15.275413185258913 38.945493595955895 33.48044736635323 42 16.552898805112402 20.664144270650493 46.32237261250016 16.46058431173694 43 14.850247959701669 18.035326122326786 45.89011675582803 21.224309162143516 44 14.564437194127244 18.829363784665578 43.25089722675367 23.355301794453506 45 16.375631083120805 21.459428511945035 46.34232030396086 15.8226201009733 46 16.38088051623647 21.259627393838468 44.980745212323065 17.378746877601998 47 15.050466998350242 22.059988828412205 45.63463711824996 17.254907054987594 48 16.043232838228025 22.288843222433215 46.95002991441875 14.717894024920012 49 13.213162257058201 18.85450249957579 40.09502303786564 27.83731220550037 50 15.66710013003901 22.29518855656697 46.06501950585176 15.972691807542263 51 16.99641810485184 23.219798111364376 44.7372191468577 15.046564636926082 52 15.410117399274542 24.218313246746494 44.81324024597943 15.558329107999533 53 16.478928199791884 23.03720083246618 45.466961498439126 15.016909469302808 54 14.585503020204124 22.63460737346386 42.90121849614664 19.87867111018538 55 16.00119732424061 23.720034357999946 44.28537962987063 15.993388687888805 56 17.42783927572616 23.581825513482578 43.54358260598099 15.446752604810282 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 17.0 7 31.0 8 90.5 9 150.0 10 207.0 11 264.0 12 312.0 13 496.0 14 632.0 15 729.0 16 826.0 17 940.5 18 1055.0 19 1322.0 20 1589.0 21 1981.0 22 2830.5 23 3288.0 24 3607.0 25 3926.0 26 4190.0 27 4454.0 28 4337.5 29 3833.0 30 3445.0 31 3031.0 32 2617.0 33 2260.0 34 1903.0 35 1809.0 36 1715.0 37 1716.5 38 1721.0 39 1724.0 40 1853.5 41 1983.0 42 2207.5 43 2432.0 44 2582.5 45 2733.0 46 2688.5 47 2619.0 48 2594.0 49 2699.5 50 2805.0 51 2935.0 52 3065.0 53 3052.0 54 3043.5 55 3048.0 56 3232.5 57 3417.0 58 3437.0 59 3457.0 60 3146.0 61 2835.0 62 2400.5 63 1718.0 64 1470.0 65 1293.0 66 1116.0 67 929.0 68 742.0 69 656.0 70 570.0 71 481.0 72 308.0 73 224.0 74 163.0 75 102.0 76 76.5 77 51.0 78 38.5 79 14.0 80 2.0 81 1.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 1.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02077625274311462 14 0.0012985157964446638 15 0.27009128566049007 16 0.06752282141512252 17 0.0012985157964446638 18 0.0012985157964446638 19 0.08829907415823714 20 0.10128423212268378 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0012985157964446638 26 0.009089610575112646 27 0.0895975899546818 28 0.11686642168001973 29 0.029865863318227267 30 0.014283673760891302 31 0.031164379114671927 32 0.08440352676890314 33 0.15582189557335965 34 0.046746568672007896 35 0.045448052875563236 36 0.036358442300450586 37 0.19218033787381025 38 0.2752853488462687 39 0.19477736946669957 40 0.6817207931334485 41 0.8479308150783654 42 0.12985157964446636 43 0.23892690654581814 44 0.5012270974276402 45 0.20646401163470154 46 0.19088182207736556 47 0.03895547389333991 48 0.16231447455558298 49 0.5168092869849762 50 0.14413525340535766 51 0.30644972796094067 52 0.12206048486579839 53 0.17010556933425094 54 0.25321058030670945 55 0.22464323278492682 56 0.17400111672358493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 77011.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.61531469530327 #Duplication Level Percentage of deduplicated Percentage of total 1 86.14736695555324 64.27912895560375 2 8.095785040548536 12.081390970121152 3 2.6330444467648184 5.893963200062329 4 1.1294420660610491 3.370947007570347 5 0.619539869827016 2.3113581176715017 6 0.3671991925098326 1.6439209982989442 7 0.23667815251818594 1.23618703821532 8 0.16184608958964183 0.9660957525548299 9 0.11659879572587101 0.7830050252561322 >10 0.46465490237026214 5.6563348093129555 >50 0.02262364693188542 1.1491864798535274 >100 0.005220841599665866 0.6284816454792173 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 269 0.34930074924361454 No Hit TTCATAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 114 0.14803080079469166 No Hit TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.13115009544091102 No Hit TTCATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 85 0.11037384269779642 No Hit TTCATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 82 0.10647829530846242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.006492578982223319 0.0 0.0 0.0 0.0 7 0.006492578982223319 0.0 0.0 0.0 0.0 8 0.006492578982223319 0.0 0.0 0.0 0.0 9 0.006492578982223319 0.0 0.0 0.0 0.0 10 0.01688070535378063 0.0 0.0 0.0 0.0 11 0.019477736946669957 0.0 0.0 0.0 0.0 12 0.023373284336003948 0.0 0.0 0.0 0.0 13 0.024671800132448612 0.0 0.0 0.0 0.0 14 0.029865863318227267 0.0 0.0 0.0 0.0 15 0.029865863318227267 0.0 0.0 0.0 0.0 16 0.03246289491111659 0.0 0.0 0.0 0.0 17 0.03505992650400592 0.0 0.0 0.0 0.0 18 0.03895547389333991 0.0 0.0 0.0 0.0 19 0.04155250548622924 0.0 0.0 0.0 0.0 20 0.046746568672007896 0.0 0.0 0.0 0.0 21 0.05194063185778655 0.0 0.0 0.0 0.0 22 0.05843321084000987 0.0 0.0 0.0 0.0 23 0.07141836880445651 0.0 0.0 0.0 0.0 24 0.08700055836179248 0.0 0.0 0.0 0.0 25 0.09609016893690511 0.0 0.0 0.0 0.0 26 0.09738868473334979 0.0 0.0 0.0 0.0 27 0.10907532690135176 0.0 0.0 0.0 0.0 28 0.11556790588357507 0.0 0.0 0.0 0.0 29 0.12206048486579839 0.0 0.0 0.0 0.0 30 0.12465751645868772 0.0 0.0 0.0 0.0 31 0.13115009544091105 0.0 0.0 0.0 0.0 32 0.13504564283024503 0.0 0.0 0.0 0.0 33 0.1363441586266897 0.0 0.0 0.0 0.0 34 0.13764267442313435 0.0 0.0 0.0 0.0 35 0.138941190219579 0.0 0.0 0.0 0.0 36 0.14023970601602367 0.0 0.0 0.0 0.0 37 0.14413525340535768 0.0 0.0 0.0 0.0 38 0.146732284998247 0.0 0.0 0.0 0.0 39 0.14803080079469166 0.0 0.0 0.0 0.0 40 0.14803080079469166 0.0 0.0 0.0 0.0 41 0.154523379776915 0.0 0.0 0.0 0.0 42 0.15971744296269363 0.0 0.0 0.0 0.0 43 0.1610159587591383 0.0 0.0 0.0 0.0 44 0.16361299035202764 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCACA 30 9.771393E-7 50.418148 39 CGCCAAA 15 0.0081289215 50.418148 38 TACGCCC 20 3.9916649E-4 50.418144 38 CGTCTCA 15 0.008150087 50.38502 41 GTCTTGC 20 4.0046475E-4 50.38502 41 GTACGCC 20 4.043797E-4 50.285904 37 TCTCGTT 15 0.00825653 50.22004 44 CCAACAC 20 4.0700653E-4 50.22004 43 ACCAACA 15 0.008277942 50.187176 42 CAACGAT 25 2.0395726E-5 50.12157 46 CGTGCAA 15 0.00832089 50.12157 48 ATCAGGC 15 0.00832089 50.12157 49 ATATGCC 25 2.0395726E-5 50.12157 34 CGATTAA 15 0.00832089 50.12157 49 AAGATTA 55 0.0 50.12157 48 AGTTCTG 85 0.0 50.121567 45 GGTACCG 15 0.008364004 50.056137 15 AGGAATC 15 0.008364004 50.056137 15 TAGCCAG 15 0.008364004 50.056137 50 TGATTGT 15 0.008364004 50.056137 15 >>END_MODULE