FastQCFastQC Report
Fri 17 Jun 2016
SRR1526208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13556
Sequences flagged as poor quality0
Sequence length56
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT590.4352316317497787No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.24343464148716434No Hit
CTCCATGGGGTCTGGTGTTCACCCTGTTTCTCATAGCCTCGTTGTGTGGCTTTGGC330.24343464148716434No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA300.22130421953378576No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG210.15491295367365004No Hit
CTCCATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA190.14015933903806432No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13278253172027146No Hit
CTCCATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11802891708468574No Hit
CTCCATGGGGTCTGGTGTTCACCCTGTTTCTCATAGCCTCCTTGTGTGGCTTTGGC160.11802891708468574No Hit
CTCCATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11802891708468574No Hit
CTCCATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11802891708468574No Hit
CTCCATGGGCGTCTACTTTGGCCTTCTCCAGACCTTCCCCTTCATCATGCTGACGT160.11802891708468574No Hit
CTCCATGGGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11065210976689288No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA140.10327530244910003No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC140.10327530244910003No Hit
CTCCATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA140.10327530244910003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCA150.00825764549.9925915
TGTTCAC150.00825764549.9925916
TCACCCT150.00825764549.9925919
GTTCACC204.0405215E-449.99258817
GGGCAGT354.9358277E-849.808127
CATGGGC1500.049.808124
ATGGGGT950.049.8081175
ATGGGCT550.049.8081175
ATGGGCG150.00837986849.8081175
GGATGGT309.987452E-749.8081178
GGGTCTG150.00837986849.8081178
GGGCAGA150.00837986849.8081177
CCTGTTT150.00837986849.80811723
TCTGGTG150.00837986849.80811711
TTTCTCA150.00837986849.80811727
GGTCTGG150.00837986849.8081179
TGTTTCT150.00837986849.80811725
GTTTCTC150.00837986849.80811726
GACATGG150.00837986849.8081179
TGGGGTC309.987452E-749.8081176
Produced by FastQC (version 0.11.5)