FastQCFastQC Report
Fri 17 Jun 2016
SRR1526207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48921
Sequences flagged as poor quality0
Sequence length56
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1110.2268964248482247No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC800.16352895484556734No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.1246908280697451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAC150.00820652650.26935241
CCTCATA150.00820652650.26935241
CAAATAT150.00820652650.26935234
AATCCTA150.00830756150.114213
GCCAGGA150.00830756150.114215
CATTATC150.00830756150.114213
AAGAAGA150.00830756150.114248
ACAAATA150.008375430550.011333
ACTAATC150.008375430550.011310
AACCGTG150.008375430550.011310
ACGCCGT150.008375430550.011311
CTAATCC150.008375430550.011311
TAATCCT150.008375430550.011312
AGCATGT150.008375430550.011311
AACAGCC150.008375430550.011311
GCATTAT150.008375430550.011312
GCATTCG204.1396247E-450.01129512
ATCCTCT150.00840951949.96000332
ATTATAT150.00840951949.96000350
GAGTGTC150.0084437149.9088139
Produced by FastQC (version 0.11.5)