##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526207_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48921 Sequences flagged as poor quality 0 Sequence length 56 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.94272398356534 39.0 38.0 40.0 35.0 40.0 2 37.78283354796508 39.0 38.0 40.0 35.0 40.0 3 36.00582571901637 39.0 37.0 40.0 27.0 40.0 4 35.763046544428775 39.0 36.0 40.0 23.0 40.0 5 37.245272991148994 39.0 38.0 40.0 33.0 40.0 6 37.65558757997588 39.0 38.0 40.0 35.0 40.0 7 37.815559780053555 39.0 38.0 40.0 35.0 40.0 8 37.668956072034504 39.0 38.0 40.0 34.0 40.0 9 37.597657447721836 39.0 38.0 40.0 34.0 40.0 10 37.04580854847612 39.0 38.0 40.0 33.0 40.0 11 36.5847999836471 38.0 36.0 40.0 31.0 40.0 12 34.693178798471 38.0 34.0 39.0 23.0 40.0 13 34.30301915332883 38.0 33.0 39.0 22.0 40.0 14 34.4082295946526 38.0 33.0 39.0 22.0 40.0 15 34.36156251916355 38.0 33.0 39.0 22.0 40.0 16 33.69199321354837 38.0 32.0 39.0 21.0 40.0 17 33.68907013348051 38.0 31.0 39.0 22.0 40.0 18 33.13720079311543 37.0 31.0 39.0 19.0 40.0 19 33.99491016128043 38.0 32.0 39.0 23.0 40.0 20 34.130353018131274 38.0 32.0 39.0 24.0 40.0 21 34.92383638928068 38.0 34.0 39.0 26.0 40.0 22 34.597861858915394 38.0 33.0 39.0 24.0 40.0 23 35.201385907892316 38.0 34.0 39.0 27.0 40.0 24 34.75333701273482 38.0 33.0 39.0 25.0 40.0 25 35.0859549068907 38.0 34.0 39.0 26.0 40.0 26 35.7378426442632 38.0 35.0 39.0 29.0 40.0 27 35.660554771979314 38.0 35.0 39.0 28.0 40.0 28 35.70525950001022 38.0 35.0 39.0 29.0 40.0 29 35.496412583553074 38.0 35.0 39.0 28.0 40.0 30 35.44514625620899 38.0 35.0 39.0 28.0 40.0 31 35.82747695263792 38.0 35.0 39.0 30.0 40.0 32 35.51000592792461 38.0 35.0 39.0 28.0 40.0 33 35.707937286645816 38.0 35.0 39.0 29.0 40.0 34 35.714274033646085 38.0 35.0 39.0 29.0 40.0 35 35.57071605241103 38.0 35.0 39.0 29.0 40.0 36 34.94200854438789 38.0 35.0 39.0 29.0 40.0 37 34.69974039778418 38.0 34.0 39.0 28.0 40.0 38 34.44318390875085 38.0 33.0 39.0 27.0 40.0 39 33.95220866294638 37.0 33.0 39.0 25.0 40.0 40 30.625109871016537 35.0 27.0 39.0 10.0 40.0 41 27.19020461560475 32.0 16.0 38.0 6.0 39.0 42 31.475807935242536 35.0 30.0 38.0 15.0 40.0 43 30.00940291490362 35.0 27.0 38.0 7.0 39.0 44 29.916538909670695 35.0 27.0 38.0 6.0 39.0 45 31.192943725598415 35.0 30.0 38.0 10.0 40.0 46 30.929028433597022 35.0 29.0 38.0 4.0 40.0 47 30.451503444328612 35.0 28.0 38.0 4.0 40.0 48 30.540217902332333 35.0 28.0 38.0 4.0 40.0 49 27.84231720529016 34.0 17.0 38.0 4.0 40.0 50 30.174280983626662 35.0 27.0 38.0 4.0 40.0 51 30.466057521309867 36.0 29.0 39.0 4.0 40.0 52 30.291776537683205 35.0 29.0 39.0 4.0 40.0 53 29.933525479855277 35.0 28.0 39.0 4.0 40.0 54 28.85368246765193 35.0 23.0 39.0 4.0 40.0 55 29.277713047566486 35.0 27.0 39.0 4.0 40.0 56 27.90668629014125 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.48133579201635257 1 2 0.7760847202429844 1 3 3.0808320972383285 1 4 3.470392085935721 1 5 1.306204036716089 1 6 0.8873411642617981 1 7 0.5556333836439507 1 8 0.8449667569598134 1 9 0.81709932544981 1 10 0.83982559249975 1 11 -2.4303443551648414 1 12 -12.38845307041706 1 13 -6.632851521930412 1 14 -5.995807970001387 1 15 -7.7689084565333495 1 16 -23.097964633631314 1 17 -4.247041984202447 1 18 -11.937744683890248 1 19 -4.752688355133294 1 20 -5.512740268862498 1 21 -5.834915761695459 1 22 -2.113875047286996 1 23 -2.8237894678373294 1 24 -3.546280316775679 1 25 -2.406538424162772 1 26 2.090586339114978 1 27 1.7803255695104099 1 28 1.996771781515946 1 29 2.235755151367222 1 30 2.5059893945187284 1 31 1.5637705796498906 1 32 1.126094968755183 1 33 1.248703481414239 1 34 0.9207536555740035 1 35 0.9445978058335811 1 36 1.3225848874658013 1 37 1.7659099205407855 1 38 0.831499544764938 1 39 2.408728415709355 1 40 4.624047989917742 1 41 -8.398236540392553 1 42 -9.111126407726783 1 43 -16.104975241497073 1 44 -22.217833365864074 1 45 -10.098510862767885 1 46 -16.600513872410993 1 47 -23.931877339335124 1 48 -12.77231038725153 1 49 -19.825622421244297 1 50 -12.62641215897721 1 51 -9.010381487240974 1 52 -9.219582316864475 1 53 -9.027186055392352 1 54 -8.692006031857318 1 55 -8.880227941909627 1 56 -2.817940031275711 2 1 1.3545752286360724 2 2 1.4754533457893615 2 3 3.648826269166449 2 4 4.221484849413137 2 5 1.846029194559705 2 6 1.4835043502744298 2 7 1.3569932670825153 2 8 1.6133008997475713 2 9 1.7797025308893453 2 10 2.056921270013106 2 11 1.4686357322562387 2 12 -1.9559615696885544 2 13 -1.8504328721003986 2 14 3.336392127133145 2 15 3.2471187411354236 2 16 2.724036337714004 2 17 3.0902576758266918 2 18 -2.094616951981557 2 19 3.4316244180575737 2 20 2.3986244713026323 2 21 2.484171173710081 2 22 2.633575171265697 2 23 2.353966724489041 2 24 0.7851912713739111 2 25 1.6954528337294121 2 26 2.5114216960843834 2 27 2.6882906010791316 2 28 2.50697090730516 2 29 2.536386525820845 2 30 2.4914192148198424 2 31 1.378000788489139 2 32 1.6503786015866524 2 33 1.179252291516228 2 34 1.4824340829659448 2 35 1.3685900350710725 2 36 1.5406519102924179 2 37 1.9664927277287347 2 38 2.0340250425794153 2 39 2.8851732918628272 2 40 6.353771156503463 2 41 -5.641072868707269 2 42 -6.404229856002644 2 43 -13.469472570297462 2 44 -13.430557989467767 2 45 -5.120608968644522 2 46 -9.685749423649458 2 47 -17.436976902229734 2 48 -7.762596934118939 2 49 -11.016734611627978 2 50 -6.710190692245789 2 51 -0.5679336970515614 2 52 -0.4323069404681661 2 53 -0.3054764876410161 2 54 0.17661951452442182 2 55 -0.154390156576941 2 56 3.3078491867913122 3 1 1.1618913475719097 3 2 1.7143031110475775 3 3 3.5291079593072965 3 4 4.154779059115803 3 5 1.879479898785057 3 6 1.6411725818863232 3 7 1.5829322342186671 3 8 1.4632617761169016 3 9 1.7816587124229954 3 10 1.5500746346453482 3 11 2.3234870624596837 3 12 3.7513936728779598 3 13 3.3326657194488973 3 14 2.918942987853015 3 15 3.5419152982559226 3 16 4.313433833801639 3 17 4.565218552196022 3 18 4.9736537835427015 3 19 3.5231737138322217 3 20 4.05766202998808 3 21 3.186635962442473 3 22 3.20125905232986 3 23 3.331861873158836 3 24 3.555731177477192 3 25 2.918653146718455 3 26 2.2543794425632555 3 27 2.7199807419242035 3 28 2.9584576052707305 3 29 2.978092316118179 3 30 2.4595334558214077 3 31 2.815207361259091 3 32 2.740558570287746 3 33 2.411059803253316 3 34 2.015102314577838 3 35 2.2506322885922003 3 36 3.347968029228255 3 37 2.6883237136442304 3 38 3.28695931487988 3 39 4.398670944444987 3 40 7.986595882638049 3 41 6.929349666503999 3 42 3.2624368106640205 3 43 -1.1021016782786823 3 44 1.5177988180439712 3 45 3.933577259837481 3 46 0.8923022195430299 3 47 -5.107164695656966 3 48 2.4762528311392735 3 49 3.2897990346944006 3 50 1.1724384157354315 3 51 2.199867554904621 3 52 2.9882720892657915 3 53 2.5063388488222067 3 54 4.3458292171848285 3 55 3.04122799678769 3 56 4.964148091329655 4 1 1.4372181449575265 4 2 1.5844011502632682 4 3 3.5144020769543545 4 4 3.933373830357631 4 5 1.57800931054976 4 6 1.54149937927194 4 7 1.6245989008853314 4 8 1.66342517480971 4 9 1.7146652483707072 4 10 1.740434111769531 4 11 1.7679315069041266 4 12 3.8706747186295942 4 13 3.8914892488606654 4 14 3.103583510728832 4 15 3.8548237949879507 4 16 4.4841854677885635 4 17 5.1697937155947145 4 18 4.74734659400022 4 19 4.317291360891048 4 20 3.7610934025370995 4 21 3.16457713891306 4 22 4.124461666708946 4 23 3.630064487537922 4 24 3.5581821578693535 4 25 2.8688165454112635 4 26 2.3426147366809005 4 27 1.9552748595712615 4 28 2.2493072784733457 4 29 2.177438721346938 4 30 1.18093868457958 4 31 1.0627563808669294 4 32 1.6139245833596405 4 33 1.2223343130572388 4 34 0.49631146490463607 4 35 1.3021028768274974 4 36 2.535059532496227 4 37 2.7618531254089334 4 38 2.9364691188014476 4 39 3.651121924837149 4 40 7.578916144075961 4 41 9.546996725327528 4 42 5.889887791056182 4 43 6.495937537407592 4 44 6.601132151377303 4 45 5.663152423236173 4 46 5.889851239150872 4 47 5.030090206303818 4 48 5.405174399766725 4 49 8.009570276524464 4 50 5.089105082402099 4 51 3.5519917379111554 4 52 3.7905596709651377 4 53 4.233463031828741 4 54 4.418541635485486 4 55 3.8859992386177566 4 56 5.252546784140112 5 1 1.0484999711208332 5 2 1.4935319667689697 5 3 3.2679139294565474 5 4 4.179861977855438 5 5 1.254479898785057 5 6 1.6117364293158403 5 7 1.2962406919301088 5 8 1.6015287744585294 5 9 1.2489058102671038 5 10 1.0117247175640856 5 11 1.8686777755608404 5 12 4.152720372712125 5 13 3.9595313910906853 5 14 3.503934695979055 5 15 3.517246973214462 5 16 3.8024471007999807 5 17 4.959082565463021 5 18 5.223861080391785 5 19 3.9037707287575927 5 20 3.9687316817293734 5 21 3.016859843039491 5 22 3.6560683392287032 5 23 3.165402503341255 5 24 3.4879451078254533 5 25 3.079308204761574 5 26 1.5267675022647467 5 27 2.044607607595843 5 28 2.073714653363602 5 29 2.5033825317069045 5 30 2.3598236714101333 5 31 1.3941874607615787 5 32 1.0239333629908955 5 33 1.123124479870235 5 34 1.6703676545446697 5 35 0.8756322885922003 5 36 1.906218443821949 5 37 2.5353386390173682 5 38 1.9617105586609753 5 39 3.24506397927086 5 40 6.961927557596589 5 41 9.280095935160713 5 42 5.058456711161533 5 43 6.428992849084501 5 44 6.104863494660886 5 45 5.750948735791045 5 46 6.26232709516492 5 47 4.90651689638284 5 48 6.509213030144249 5 49 8.277568520598216 5 50 5.674925977924488 5 51 5.78838330946515 5 52 5.670278722764962 5 53 6.398959080994679 5 54 5.300016613536403 5 55 5.2286243483631445 5 56 4.8449524031041165 6 1 1.5248140528859153 6 2 1.8755349951055535 6 3 4.058093466553672 6 4 4.7809867885844 6 5 2.182016130669112 6 6 1.9660518089394614 6 7 1.7550336834940268 6 8 1.882947766455068 6 9 1.9060548476886936 6 10 2.3030939583168433 6 11 2.394743101107025 6 12 4.7127463298827905 6 13 4.984839632492374 6 14 4.4104888815728245 6 15 4.878267955260753 6 16 5.146163302400673 6 17 5.446861064273314 6 18 6.270151368083766 6 19 5.356507047165557 6 20 3.9797634792634398 6 21 3.960186687080153 6 22 3.999568231073823 6 23 4.327634820018744 6 24 4.742325380375746 6 25 3.7658753689406765 6 26 3.555104080244412 6 27 3.419256104243047 6 28 2.9415493927103427 6 29 3.672536760562629 6 30 3.2391228278020847 6 31 2.4655696800996694 6 32 1.9984088118336416 6 33 1.7389583539779565 6 34 2.0664308169932966 6 35 2.1944728683023484 6 36 2.913789285266901 6 37 3.2281787861079962 6 38 3.2742781554595908 6 39 3.885989785024698 6 40 7.511354336744333 6 41 2.8351467679532725 6 42 5.508813622258224 6 43 7.560941799982189 6 44 6.372871281812085 6 45 5.4909444095959365 6 46 5.238316712296651 6 47 5.961675884053179 6 48 5.970818048530578 6 49 7.082673430829665 6 50 5.5492500099383335 6 51 6.4963651394456825 6 52 6.197209287333429 6 53 5.145227737711096 6 54 7.794500714769367 6 55 6.147508190024407 6 56 6.06861669036347 7 1 1.2749865856671434 7 2 1.4821602539047234 7 3 3.695774625973961 7 4 4.40279493213167 7 5 1.4132100575152151 7 6 1.581648772362513 7 7 1.3865036627900906 7 8 1.9215951094502373 7 9 1.3808650616293434 7 10 1.913169872740582 7 11 2.08142357039619 7 12 3.7513936728779598 7 13 4.205681592464771 7 14 3.88322870213873 7 15 4.361359742700365 7 16 5.144783040150841 7 17 4.842996329973801 7 18 4.884368069256986 7 19 3.9596816503401584 7 20 4.071550918876966 7 21 4.141001041807556 7 22 4.078243179313986 7 23 3.2227348890318552 7 24 3.248191494937508 7 25 2.2738118768771827 7 26 1.7504111885950024 7 27 1.700139472082931 7 28 2.2044893513024775 7 29 2.767774855800724 7 30 2.2750096462976046 7 31 2.5711597422114707 7 32 1.7306379353671133 7 33 2.075742342935861 7 34 1.5170864415619647 7 35 1.8915053044652197 7 36 1.461063267323489 7 37 1.922450697771211 7 38 2.0389434418640064 7 39 2.666528087302133 7 40 6.266357787399954 7 41 -0.8563646192102858 7 42 3.0560876043148184 7 43 4.679644353467346 7 44 4.7558940561392085 7 45 3.5744502757104932 7 46 4.426032378273192 7 47 4.27775593926367 7 48 4.450459180345629 7 49 7.021941891837258 7 50 4.188311431608447 7 51 4.491534221571289 7 52 4.7858911368848425 7 53 4.938878531361887 7 54 6.4390831854388 7 55 4.930116885676579 7 56 4.148671900853465 8 1 1.2908596015401628 8 2 1.4980332697777357 8 3 3.5370444672438026 8 4 4.164699694036436 8 5 1.603686247991405 8 6 1.2006963914101334 8 7 1.3865036627900906 8 8 1.6200078078629332 8 9 1.5872142679785455 8 10 1.7385666981374115 8 11 2.2084076973803164 8 12 4.195838117322403 8 13 4.189808576591751 8 14 4.438784257694287 8 15 3.440724822065448 8 16 4.795576690944493 8 17 4.827123314100781 8 18 4.884368069256986 8 19 4.356507047165557 8 20 4.4525032998293455 8 21 3.8711597719662834 8 22 3.7925288935997017 8 23 3.5878142541112155 8 24 4.105334352080369 8 25 3.6706372737025816 8 26 3.0996175378013504 8 27 2.8112505831940453 8 28 3.140997287810414 8 29 3.132854220880084 8 30 3.100406471694427 8 31 2.3013184723701983 8 32 2.6353998401290184 8 33 1.6630439302374427 8 34 2.1520070764825974 8 35 1.9708703838302952 8 36 2.603920410180635 8 37 2.478006253326768 8 38 3.007197410117975 8 39 3.555416976191019 8 40 6.044135565177733 8 41 -0.586523349369017 8 42 4.627516175743388 8 43 6.679644353467346 8 44 6.724148024393177 8 45 5.399847101107323 8 46 5.251429203670014 8 47 5.166644828152556 8 48 5.339348069234514 8 49 7.228291098186467 8 50 4.426406669703688 8 51 5.666137396174459 8 52 5.627160978154684 8 53 4.034116626599982 8 54 6.883527629883243 8 55 5.009481965041662 8 56 5.069306821488386 9 1 1.108454436084152 9 2 1.3167042409910792 9 3 3.1477520271039054 9 4 3.7407018463097756 9 5 1.4917680343782749 9 6 1.072905161923984 9 7 1.2686666974954974 9 8 1.4206534898645486 9 9 1.0561408216508639 9 10 1.7843025065850782 9 11 2.356914557751587 9 12 3.9566667425578075 9 13 3.9258860584319493 9 14 3.941071048116669 9 15 4.116538650421646 9 16 3.889469615345895 9 17 4.456932299841974 9 18 4.600537022714079 9 19 4.057072018916969 9 20 3.0696676797055886 9 21 2.8243478268492552 9 22 3.032238336698974 9 23 2.711569971087272 9 24 2.6708441718274756 9 25 2.6472313011440676 9 26 0.8348879171395254 9 27 1.410658708025899 9 28 1.2682504489618793 9 29 1.9663220712970926 9 30 2.2803903296443835 9 31 1.6704333499596515 9 32 1.5501160090824726 9 33 1.4795626281120775 9 34 1.1396315047849939 9 35 1.53664923774474 9 36 3.2436836601133763 9 37 2.667137272966265 9 38 3.211394343128468 9 39 3.8231059726935754 9 40 7.561219234803772 9 41 4.056468310571795 9 42 4.697465059251591 9 43 5.9981808075970235 9 44 6.054521981885781 9 45 4.424867278669868 9 46 5.628884140446985 9 47 5.3434002760944495 9 48 4.9430607407437925 9 49 7.799450635447698 9 50 5.449274573927525 9 51 4.883247969217241 9 52 4.57819675969894 9 53 5.280820958050079 9 54 6.647853699294057 9 55 6.150455868727423 9 56 6.900353364399336 10 1 1.5858134039566494 10 2 1.7174125637838955 10 3 3.4171676607998336 10 4 4.314190336064392 10 5 1.9111963166955022 10 6 1.820691653196441 10 7 1.7440018859599604 10 8 1.810483998339123 10 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 4 212.0 5 7.0 6 35.0 7 50.0 8 91.0 9 67.0 10 60.0 11 33.0 12 49.0 13 36.0 14 57.0 15 90.0 16 132.0 17 162.0 18 198.0 19 254.0 20 331.0 21 407.0 22 483.0 23 615.0 24 724.0 25 840.0 26 989.0 27 1053.0 28 1216.0 29 1455.0 30 1772.0 31 2117.0 32 2449.0 33 2735.0 34 3267.0 35 3807.0 36 4865.0 37 6449.0 38 7001.0 39 4804.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.4215163222338 0.5232926555058155 0.03883812677582224 0.016352895484556734 8 99.31113427771304 0.5887042374440424 0.04292635064696143 0.05723513419594857 9 95.711453159175 2.7963451278592015 0.8564829010036589 0.6357188119621431 10 30.332577011917174 47.65029333006275 10.11630996913391 11.900819688886163 11 26.74311645305697 31.85339629198095 22.861347887410314 18.542139367551766 12 33.38034790785143 15.343104188385356 25.563663866233316 25.712884037529893 13 23.530855109802477 17.85057048214943 27.759375127796183 30.859199280251914 14 20.85897095198185 23.747419203172594 29.634702262924428 25.758907581921136 15 23.763268986433868 22.541907455223576 27.589245460879546 26.10557809746301 16 26.321817921498848 24.95755865087644 25.4423104456853 23.27831298193942 17 25.410355471065593 24.901371599108767 23.548169497761698 26.14010343206394 18 22.834774432247908 27.061997915005826 25.36334089654749 24.73988675619877 19 24.090444035195414 29.244935543278082 23.249437282586456 23.415183138940048 20 25.38205028538696 27.73061107587815 23.528569382786767 23.35876925594812 21 25.228429508799906 25.565707978168884 25.150753255248258 24.055109257782956 22 23.916109646164223 25.614766664622557 25.966353917540523 24.502769771672696 23 23.37646409517385 26.792175139510636 27.233703317593672 22.597657447721836 24 22.530201753848043 28.05134809182151 27.061997915005826 22.356452239324625 25 24.069910057236303 28.049877350776782 27.023712183156174 20.856500408830744 26 21.8117512368647 28.086028539886332 28.94876722410762 21.15345299914135 27 20.415805520882362 24.393787472630912 32.433649143628884 22.756757862857842 28 19.77650887210659 24.391641595546552 34.75778228034629 21.07406725200057 29 20.194687007914272 24.20703899875253 35.79214298860917 19.806131004724023 30 18.56062154978532 23.915354733183396 39.088121038642406 18.435902678388878 31 18.277371068667946 24.410044578953826 40.09651956975175 17.21606478262648 32 18.35519449958051 22.531666291514046 42.4461314944034 16.667007714502034 33 18.284193284193286 22.653562653562652 42.8972153972154 16.165028665028665 34 15.685271920315792 23.320311701061502 45.93909148548872 15.055324893133987 35 15.788181873964533 23.758974044302633 47.24795974719274 13.204884334540099 36 15.892035579184133 24.091606175237708 45.54544525099683 14.470912994581331 37 15.68406610284029 23.428828865726047 46.38461695984273 14.502488071590932 38 16.53617544578807 23.638040582086493 45.63640090182414 14.189383070301293 39 16.70899037476961 23.436412041777597 44.53000204792136 15.324595535531435 40 12.350154479917611 18.38311019567456 40.85684860968074 28.409886714727083 41 11.573606154988553 17.241806068356677 37.94063653802521 33.24395123862956 42 14.451245726451983 23.93186889675927 46.22187647143121 15.395008905357546 43 12.519205949234834 20.680966136070307 46.31143342961916 20.4883944850757 44 13.073747766619432 20.99480418129916 43.634608670650806 22.296839381430598 45 14.776801261958905 24.085796816421855 46.52449142646426 14.61291049515498 46 14.262603923495925 24.794200761764344 45.97821190154401 14.964983413195723 47 14.009651956975175 24.410044578953826 45.80794241544313 15.772361048627866 48 14.321695505272858 25.95679328350568 45.831882870891775 13.889628340329683 49 12.536718637661512 21.07598447032723 40.297035804523325 26.09026108748793 50 13.902024606440255 25.44576142807427 45.976376179655674 14.6758377858298 51 14.649746817277927 26.220299719141433 44.7364644621661 14.393489001414544 52 14.293607268560201 26.68617500204633 44.624294016534336 14.39592371285913 53 13.721715982389679 27.310330705436677 44.37391215316883 14.594041159004812 54 13.411311633242518 25.33953335928058 42.438085093307656 18.811069914169245 55 14.24414434722023 27.244410746121844 44.51730568249349 13.994139224164428 56 14.786063945271696 26.985232369989554 43.559387993363785 14.669315691374967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 4.5 5 8.0 6 28.0 7 48.0 8 115.5 9 183.0 10 295.5 11 408.0 12 491.0 13 653.0 14 732.0 15 816.5 16 901.0 17 890.5 18 880.0 19 1007.0 20 1134.0 21 1459.0 22 2080.5 23 2377.0 24 2683.0 25 2989.0 26 2999.0 27 3009.0 28 2847.5 29 2431.5 30 2177.0 31 1844.5 32 1512.0 33 1446.5 34 1381.0 35 1219.0 36 1057.0 37 1032.0 38 1044.0 39 1081.0 40 1128.0 41 1175.0 42 1239.5 43 1304.0 44 1304.0 45 1304.0 46 1361.0 47 1448.0 48 1478.0 49 1553.5 50 1629.0 51 1672.5 52 1716.0 53 1659.0 54 1730.0 55 1858.0 56 1997.5 57 2137.0 58 2013.0 59 1889.0 60 1774.0 61 1659.0 62 1458.0 63 1047.0 64 837.0 65 702.0 66 567.0 67 500.5 68 434.0 69 367.0 70 300.0 71 262.0 72 165.5 73 107.0 74 79.5 75 52.0 76 35.5 77 19.0 78 17.5 79 11.5 80 7.0 81 4.0 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.030661679033543877 14 0.0040882238711391836 15 0.2514257680750598 16 0.06132335806708775 17 0.0 18 0.0 19 0.10424970871404918 20 0.08176447742278367 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0020441119355695918 26 0.014308783548987142 27 0.10629382064961879 28 0.1226467161341755 29 0.04497046258253102 30 0.02248523129126551 31 0.03883812677582224 32 0.10629382064961879 33 0.16557306678113692 34 0.05723513419594857 35 0.06132335806708775 36 0.03270579096911347 37 0.17988185033012408 38 0.26777866355961655 39 0.18601418613683285 40 0.7583655280963186 41 0.8994092516506205 42 0.1512642832321498 43 0.2207640890415159 44 0.4660575213098669 45 0.2207640890415159 46 0.1778377383945545 47 0.03883812677582224 48 0.17579362645898491 49 0.490586864536702 50 0.1471760593610106 51 0.29026389485088205 52 0.10833793258518837 53 0.17579362645898491 54 0.21258764129923757 55 0.2493816561394902 56 0.20032296968582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 48921.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.27033380347909 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56608641338293 64.4058788659267 2 8.486543736251798 12.775699597309947 3 2.6070662357765526 5.8870423744404246 4 1.2655134019498682 3.810224647901719 5 0.7305216848165549 2.749330553341101 6 0.4073540993400864 1.8397007420126326 7 0.24169676560845127 1.2734817358598556 8 0.1710887217228363 1.0302324155270743 9 0.09233359585041957 0.6254982522842951 >10 0.4236482633136899 5.087794607632714 >50 0.005431387991201151 0.2882197829153125 >100 0.0027156939956005754 0.2268964248482247 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 111 0.2268964248482247 No Hit TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 80 0.16352895484556734 No Hit TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.1246908280697451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0020441119355695918 0.0 0.0 0.0 0.0 11 0.0020441119355695918 0.0 0.0 0.0 0.0 12 0.008176447742278367 0.0 0.0 0.0 0.0 13 0.008176447742278367 0.0 0.0 0.0 0.0 14 0.01226467161341755 0.0 0.0 0.0 0.0 15 0.014308783548987142 0.0 0.0 0.0 0.0 16 0.014308783548987142 0.0 0.0 0.0 0.0 17 0.018397007420126326 0.0 0.0 0.0 0.0 18 0.028617567097974285 0.0 0.0 0.0 0.0 19 0.030661679033543877 0.0 0.0 0.0 0.0 20 0.030661679033543877 0.0 0.0 0.0 0.0 21 0.03679401484025265 0.0 0.0 0.0 0.0 22 0.03883812677582225 0.0 0.0 0.0 0.0 23 0.04497046258253102 0.0 0.0 0.0 0.0 24 0.053146910324809386 0.0 0.0 0.0 0.0 25 0.06336747000265734 0.0 0.0 0.0 0.0 26 0.0776762535516445 0.0 0.0 0.0 0.0 27 0.08585270129392286 0.0 0.0 0.0 0.0 28 0.10016148484291 0.0 0.0 0.0 0.0 29 0.10833793258518837 0.0 0.0 0.0 0.0 30 0.11038204452075796 0.0 0.0 0.0 0.0 31 0.11651438032746674 0.0 0.0 0.0 0.0 32 0.11855849226303633 0.0 0.0 0.0 0.0 33 0.1226467161341755 0.0 0.0 0.0 0.0 34 0.1226467161341755 0.0 0.0 0.0 0.0 35 0.12673494000531468 0.0 0.0 0.0 0.0 36 0.12673494000531468 0.0 0.0 0.0 0.0 37 0.13695549968316265 0.0 0.0 0.0 0.0 38 0.13899961161873226 0.0 0.0 0.0 0.0 39 0.14308783548987142 0.0 0.0 0.0 0.0 40 0.1471760593610106 0.0 0.0 0.0 0.0 41 0.1471760593610106 0.0 0.0 0.0 0.0 42 0.15330839516771938 0.0 0.0 0.0 0.0 43 0.16148484290999776 0.0 0.0 0.0 0.0 44 0.16352895484556734 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTAC 15 0.008206526 50.269352 41 CCTCATA 15 0.008206526 50.269352 41 CAAATAT 15 0.008206526 50.269352 34 AATCCTA 15 0.008307561 50.1142 13 GCCAGGA 15 0.008307561 50.1142 15 CATTATC 15 0.008307561 50.1142 13 AAGAAGA 15 0.008307561 50.1142 48 ACAAATA 15 0.0083754305 50.0113 33 ACTAATC 15 0.0083754305 50.0113 10 AACCGTG 15 0.0083754305 50.0113 10 ACGCCGT 15 0.0083754305 50.0113 11 CTAATCC 15 0.0083754305 50.0113 11 TAATCCT 15 0.0083754305 50.0113 12 AGCATGT 15 0.0083754305 50.0113 11 AACAGCC 15 0.0083754305 50.0113 11 GCATTAT 15 0.0083754305 50.0113 12 GCATTCG 20 4.1396247E-4 50.011295 12 ATCCTCT 15 0.008409519 49.960003 32 ATTATAT 15 0.008409519 49.960003 50 GAGTGTC 15 0.00844371 49.908813 9 >>END_MODULE