FastQCFastQC Report
Fri 17 Jun 2016
SRR1526206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82206
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10351.2590321874315742No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3070.37345205946038No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2760.33574191664841985No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2600.3162786171325694No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1430.1739532394229132No Hit
TAGAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG980.11921270953458385No Hit
TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG940.11434688465562125No Hit
TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG870.10583169111743668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTAC150.008054750.53827338
GCGTAGA251.9431147E-550.53827338
GCCTGTC550.050.5382738
CTGTACC150.00807438850.50709539
AGCGTAG309.702544E-750.47595637
TGCTCGT550.050.47595637
TAAAGGC150.00821321550.28992534
CGATTCA204.1055508E-450.1359549
ACGATTC204.1055508E-450.1359548
CGATTAA252.0373354E-550.13594449
ACGATTA252.0373354E-550.13594448
CAACGAT402.581146E-950.10526346
TCGCACT150.00835380850.0746242
ACCCGAC150.00835380850.0746242
GGTCCTA150.008414607549.98290650
GATTCAG301.0383337E-649.98290650
CTTAATC301.0427211E-649.9524115
GACAGTC150.00843494749.952419
GATGTAC150.00843494749.952419
GCATTAT301.0427211E-649.9524112