##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526206_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82206 Sequences flagged as poor quality 0 Sequence length 56 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.7326350874632 39.0 38.0 39.0 34.0 40.0 2 36.55806145536822 39.0 38.0 39.0 31.0 40.0 3 37.08882563316546 39.0 37.0 39.0 33.0 40.0 4 35.95405444858039 39.0 36.0 39.0 29.0 40.0 5 36.697905262389604 39.0 36.0 39.0 31.0 40.0 6 34.96742330243534 38.0 35.0 39.0 20.0 40.0 7 37.228997883366176 39.0 38.0 39.0 33.0 40.0 8 37.16850351555848 39.0 38.0 39.0 33.0 40.0 9 37.17363696080578 39.0 38.0 39.0 33.0 40.0 10 36.308006714838335 39.0 35.0 39.0 30.0 40.0 11 35.38801304041068 38.0 34.0 39.0 28.0 40.0 12 33.882161885993725 38.0 32.0 39.0 21.0 40.0 13 33.639296401722504 38.0 32.0 39.0 20.0 40.0 14 33.664160766854 38.0 32.0 39.0 20.0 40.0 15 33.634089969102014 38.0 32.0 39.0 20.0 40.0 16 33.51914702089872 38.0 31.0 39.0 21.0 40.0 17 33.50200715276257 37.0 31.0 39.0 21.0 40.0 18 32.939700265187454 37.0 30.0 39.0 19.0 40.0 19 33.85308858234192 37.0 31.0 39.0 23.0 40.0 20 33.85771111597693 37.0 31.0 39.0 23.0 40.0 21 34.74459285210325 38.0 33.0 39.0 26.0 40.0 22 34.48542685448751 38.0 33.0 39.0 25.0 40.0 23 34.97509914118191 38.0 34.0 39.0 26.0 40.0 24 34.563255723426515 38.0 33.0 39.0 25.0 40.0 25 34.82961097730092 38.0 33.0 39.0 26.0 40.0 26 35.56662530715519 38.0 35.0 39.0 29.0 40.0 27 35.47250808943386 38.0 35.0 39.0 29.0 40.0 28 35.33326034596015 38.0 35.0 39.0 28.0 40.0 29 35.208622241685525 38.0 35.0 39.0 27.0 40.0 30 35.07658808359487 38.0 35.0 39.0 27.0 40.0 31 35.462496654745394 38.0 35.0 39.0 29.0 40.0 32 35.149806583461064 38.0 35.0 39.0 27.0 40.0 33 35.317044984551 38.0 35.0 39.0 29.0 40.0 34 35.19592243875143 38.0 35.0 39.0 28.0 40.0 35 35.15406418023015 38.0 35.0 39.0 28.0 40.0 36 34.410432328540494 38.0 33.0 39.0 28.0 40.0 37 34.133566892927526 37.0 33.0 39.0 27.0 40.0 38 33.803165219083766 37.0 33.0 39.0 26.0 40.0 39 33.34042527309442 36.0 32.0 39.0 23.0 40.0 40 29.91260978517383 35.0 25.0 38.0 9.0 39.0 41 26.2922049485439 31.0 15.0 38.0 5.0 39.0 42 30.83475658711043 35.0 29.0 38.0 10.0 39.0 43 29.284273653991193 34.0 26.0 38.0 5.0 39.0 44 29.230944213317763 34.0 26.0 38.0 4.0 39.0 45 30.378719314891857 35.0 29.0 38.0 4.0 39.0 46 29.93983407537163 35.0 27.0 38.0 4.0 40.0 47 29.42625842395932 35.0 27.0 38.0 4.0 39.0 48 29.52527796024621 35.0 27.0 38.0 4.0 39.0 49 26.9348101111841 33.0 15.0 38.0 4.0 39.0 50 29.039036080091478 35.0 26.0 38.0 4.0 39.0 51 29.1993528452911 35.0 26.0 39.0 4.0 40.0 52 29.016118044911565 35.0 26.0 39.0 4.0 40.0 53 28.582001313772718 35.0 23.0 39.0 4.0 40.0 54 27.574994525947012 34.0 17.0 39.0 4.0 40.0 55 27.88849962289857 35.0 21.0 39.0 4.0 40.0 56 26.522820718682336 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6457380688078516 1 2 1.790992368249178 1 3 1.235661655741076 1 4 2.2431865856942466 1 5 1.3376399481813763 1 6 3.353274222635484 1 7 1.1485240218062245 1 8 1.17768467900612 1 9 1.2518312265518716 1 10 1.013195122471302 1 11 -4.20634853019919 1 12 -14.392661972339774 1 13 -10.71029350898539 1 14 -6.744521545046215 1 15 -7.634861955651743 1 16 -23.64013723840963 1 17 -5.40746928292068 1 18 -13.97281358163628 1 19 -6.317909362546267 1 20 -6.169958268294156 1 21 -5.928460413563357 1 22 -3.343804460415747 1 23 -3.4567602389367913 1 24 -4.6463980161286464 1 25 -3.5732502548888583 1 26 1.7216190319008078 1 27 1.9180922921151975 1 28 2.0505308483958586 1 29 1.473394556988545 1 30 1.484458937489869 1 31 1.138358790473177 1 32 1.4304890081105626 1 33 0.8799066365474104 1 34 0.8918370900004007 1 35 1.3975537434564131 1 36 0.9774208160518754 1 37 1.262622466294836 1 38 1.0160617158119791 1 39 2.052417706119904 1 40 5.806679349758696 1 41 -9.010024878262296 1 42 -9.069520750736828 1 43 -16.840624696349813 1 44 -22.248669260689514 1 45 -11.030230796004606 1 46 -16.005296088578593 1 47 -22.65189723756057 1 48 -13.792850843740892 1 49 -19.530124249650918 1 50 -12.657824824101233 1 51 -8.907945787714695 1 52 -8.773445665261878 1 53 -9.070633831010984 1 54 -8.257525235389508 1 55 -8.552537177679007 1 56 -3.816302770561741 2 1 1.309178929022906 2 2 2.3210998951308994 2 3 1.8090487525152739 2 4 2.617111316877036 2 5 1.7481238191491144 2 6 4.21940325489355 2 7 1.733739075569666 2 8 1.7499965069631145 2 9 1.772530151283057 2 10 1.8726037246218397 2 11 1.5573611472201634 2 12 -2.819812509974181 2 13 -0.770777379953131 2 14 2.8076289925881923 2 15 3.2723961088643847 2 16 2.2859380304075785 2 17 2.8454877063266295 2 18 -1.767168420345957 2 19 2.4345099922924405 2 20 2.3329987209531566 2 21 1.9199266832108393 2 22 2.1989374750681208 2 23 2.2295300836438514 2 24 0.592311661290708 2 25 1.9436852289821118 2 26 2.1130168813631727 2 27 2.39255465770659 2 28 2.170154504309842 2 29 1.7317278903218778 2 30 2.552469690178043 2 31 1.842659865741993 2 32 1.6240373952073384 2 33 1.9866270666549397 2 34 1.9929123588176054 2 35 1.4779300875424326 2 36 3.120969203148654 2 37 3.206170853391612 2 38 3.1437498878549874 2 39 4.094353189990869 2 40 5.902647091694178 2 41 -4.5266915449289655 2 42 -5.483230428156183 2 43 -12.418581685597127 2 44 -13.645443454237903 2 45 -4.772435097079875 2 46 -9.130296088578593 2 47 -17.087112291324008 2 48 -6.8108615964290635 2 49 -12.09302747545737 2 50 -6.174222673563598 2 51 -0.9668167554566338 2 52 -0.8984456652618782 2 53 -0.6601499600432419 2 54 0.29785110869651277 2 55 0.039667123396259996 2 56 3.7764391649221274 3 1 1.4119337499595446 3 2 2.3863891513292472 3 3 1.483773270421608 3 4 2.4875658623315857 3 5 1.5761541221794175 3 6 3.61595972872275 3 7 1.6065352188203548 3 8 1.573756837541623 3 9 1.7279709226329203 3 10 1.5456753499661957 3 11 2.4808459957050104 3 12 4.368823853662185 3 13 3.0043603610936955 3 14 3.314929268070287 3 15 3.025495282418106 3 16 4.162315440875894 3 17 4.073380268310103 3 18 4.560999623731178 3 19 3.6955981465624177 3 20 3.644224616269959 3 21 2.862350925635077 3 22 3.315397530164539 3 23 2.7127945464537717 3 24 3.02702240509236 3 25 2.6039469369710915 3 26 1.9466256967901714 3 27 2.2248549331886878 3 28 1.3192591875054305 3 29 1.9775267883935044 3 30 1.725816797616062 3 31 1.4886653753838672 3 32 1.0014478635268986 3 33 1.2023984165171981 3 34 0.6309288877432309 3 35 0.9470072225286614 3 36 2.410638624636256 3 37 2.8115427542180598 3 38 2.4051135242186206 3 39 3.469284319467455 3 40 6.997206320344318 3 41 7.8770274633355015 3 42 4.116562960273566 3 43 -2.3683062035034617 3 44 2.662821008572017 3 45 2.7090387321212255 3 46 0.7550344899338022 3 47 -4.388145349175247 3 48 0.266066778226584 3 49 2.553942221512326 3 50 0.42295363497634497 3 51 2.5530868258105848 3 52 2.449694830605889 3 53 2.3992853016647473 3 54 3.7464736982281934 3 55 2.777063817611136 3 56 3.2561223605144143 4 1 1.6097688995243757 4 2 2.6812768862813385 4 3 1.9993879855536179 4 4 2.738571493868193 4 5 2.1610294238688823 4 6 4.264683490881751 4 7 1.996939665540168 4 8 1.902872613157804 4 9 1.9295360509880695 4 10 2.0487836656247893 4 11 2.9361959554797536 4 12 4.9963379325036925 4 13 3.976641499102911 4 14 4.182112768399399 4 15 4.087661595590049 4 16 4.407250714773355 4 17 4.818717794822206 4 18 5.0562091607749835 4 19 4.18546869435734 4 20 4.416110815348432 4 21 3.3337909899954994 4 22 3.228657239079922 4 23 3.270901765059783 4 24 3.148063873680087 4 25 2.9857943735248824 4 26 2.1389755834280706 4 27 2.030091530862933 4 28 2.167573383365884 4 29 1.6326865923867757 4 30 2.217735176903709 4 31 0.8323353819661818 4 32 1.3261022919624992 4 33 1.2808011079528754 4 34 0.9274256331538879 4 35 1.4398032438846116 4 36 2.5603497341221058 4 37 2.4030735082588706 4 38 2.2731746666160504 4 39 3.487568529223907 4 40 6.804490749511288 4 41 8.933927924097581 4 42 4.826282846180096 4 43 6.1160790813645285 4 44 5.1235830944346645 4 45 4.999172572536647 4 46 4.552226035315211 4 47 4.098507177702537 4 48 4.585967312125508 4 49 7.216854530442333 4 50 4.517738978353805 4 51 3.4370917991156382 4 52 4.253103007304492 4 53 3.3722246712251938 4 54 3.5192375393749806 4 55 2.3974104862281216 4 56 3.3136810528277323 5 1 1.3531317313827813 5 2 2.56623263849373 5 3 1.71620214484566 5 4 2.579279458469962 5 5 1.957489600860029 5 6 4.202736588226884 5 7 1.934992762885301 5 8 1.8497752680250557 5 9 1.460509502315503 5 10 2.146128798368153 5 11 3.3521251590195718 5 12 4.872444127193958 5 13 4.870446808837428 5 14 5.261758786098518 5 15 5.043413807979427 5 16 4.973622396189285 5 17 4.854116024910702 5 18 4.950014470509501 5 19 4.026176658959109 5 20 4.150624089684719 5 21 2.714321963446828 5 22 3.3437014868675305 5 23 2.9257690216969507 5 24 2.5374444046535416 5 25 2.4813695947638195 5 26 1.7053472648440007 5 27 1.8265517078540867 5 28 2.017130905489779 5 29 1.9070228755726149 5 30 1.8195050884081354 5 31 1.3013619306387483 5 32 1.1402615839978978 5 33 0.9976152672449103 5 34 1.1840628012954824 5 35 0.8822811199908074 5 36 2.763889557130952 5 37 1.7659053666659474 5 38 2.653705640067379 5 39 3.186683573471697 5 40 7.229269510573239 5 41 9.827733233832099 5 42 4.923627978923459 5 43 6.655902090214081 5 44 6.858096368770944 5 45 4.5389955813861995 5 46 5.083199486642645 5 47 4.3374452307998865 5 48 4.161188551063564 5 49 6.986766034867117 5 50 4.588535438530798 5 51 3.9149679053103306 5 52 4.332749025003611 5 53 4.000543255295991 5 54 4.802423380082946 5 55 4.061127300387412 5 56 4.561468663447201 6 1 1.7910559516766256 6 2 2.8036241669755597 6 3 2.3050843512207777 6 4 3.291625879983833 6 5 2.551440971252674 6 6 4.688173481430766 6 7 2.06051901084475 6 8 2.201695536089325 6 9 2.2255236787911485 6 10 2.7123286437156935 6 11 3.852425872139257 6 12 5.189653509443296 6 13 5.341277636228099 6 14 5.414263296795312 6 15 5.70807572051487 6 16 5.2693522698897794 6 17 5.410778968462552 6 18 5.727572679977669 6 19 4.841710639541631 6 20 4.884535937781635 6 21 3.3901532204276634 6 22 3.844002199987216 6 23 3.7142388215079336 6 24 3.9495932146887682 6 25 2.8450606335128583 6 26 2.9893922858939206 6 27 2.518363719195264 6 28 2.60987942340369 6 29 3.182132421066214 6 30 2.9158192047411546 6 31 2.271462454738753 6 32 3.0188594016798262 6 33 2.599814768920311 6 34 2.6492230384292554 6 35 2.566360508254405 6 36 2.8311633779059306 6 37 2.951316484459575 6 38 3.885254742223921 6 39 3.90762179840511 6 40 8.16955647680745 6 41 5.459230785168124 6 42 6.072352096115662 6 43 6.82980019142553 6 44 6.771061400131032 6 45 4.9810438673214215 6 46 6.062665076469951 6 47 6.517013922268227 6 48 6.099413484477086 6 49 8.869917508361397 6 50 6.268576679187213 6 51 5.640545704090297 6 52 6.352767926971129 6 53 6.298506997885557 6 54 6.649145607078392 6 55 6.468550618541894 6 56 5.934610685957729 7 1 1.0345476605863269 7 2 1.7520733464583316 7 3 1.2206269236067158 7 4 2.3226422903283677 7 5 1.2849232291786166 7 6 3.547869331589716 7 7 1.3509219664251262 7 8 1.54004075475072 7 9 1.2923679093951534 7 10 1.862942957660188 7 11 2.3875233891080683 7 12 4.748550321884224 7 13 4.109384861934771 7 14 4.500696839195861 7 15 4.494741241607748 7 16 4.292206466985746 7 17 4.50898328154787 7 18 4.649129514757284 7 19 3.813787278428137 7 20 4.026730284374985 7 21 2.369189220083996 7 22 2.441046619610894 7 23 2.46559203054651 7 24 2.6347895373969052 7 25 2.6318120726399314 7 26 1.1478251409501965 7 27 1.6495605574116041 7 28 1.8489893125694294 7 29 1.8716246454841183 7 30 1.7221599556647718 7 31 1.1597690102847622 7 32 1.4942438848828488 7 33 0.7498276566254418 7 34 1.5911424473131817 7 35 1.4221041288403669 7 36 2.0824736279274134 7 37 2.4738699684358565 7 38 2.3174224542266657 7 39 3.2220818035601937 7 40 6.406260661015715 7 41 0.11091907454006389 7 42 4.0652208992774455 7 43 5.7532472229574445 7 44 5.990839731602804 7 45 4.547845138908325 7 46 4.82656231850105 7 47 5.850719567083068 7 48 5.258533683806927 7 49 7.721279309203396 7 50 5.084110659769735 7 51 4.08310949823068 7 52 3.306200352437237 7 53 3.283729096003956 7 54 4.333396831410379 7 55 2.972631725166174 7 56 4.207486362562246 8 1 1.4214113449250405 8 2 2.6155953079749352 8 3 1.817687896246163 8 4 2.520092968253188 8 5 1.9764877335222053 8 6 4.3862228267589956 8 7 1.993448555691991 8 8 1.9571065987062326 8 9 1.9221478882861192 8 10 1.9472979142242863 8 11 3.060495703795084 8 12 5.106104921218481 8 13 4.9291461674474775 8 14 4.924907280049965 8 15 5.443267668497413 8 16 4.566136807165989 8 17 4.787460183390856 8 18 5.38153188546444 8 19 4.420672071803146 8 20 4.101423797405751 8 21 3.5495902917735265 8 22 3.419044508707266 8 23 3.4017777900658714 8 24 3.944605239272363 8 25 2.8170032717955635 8 26 2.1448211627087375 8 27 2.642172394709654 8 28 2.1292523636370575 8 29 3.1981651991903775 8 30 3.0504054699945584 8 31 1.9267577250692085 8 32 2.098043511415284 8 33 1.8197310421901065 8 34 2.5507105239552246 8 35 2.5848872740867748 8 36 2.4737214967266326 8 37 2.38277635797877 8 38 2.548566401616455 8 39 3.10322169151992 8 40 6.463579813406717 8 41 -3.0620126458463943 8 42 3.4587420489080394 8 43 4.884935133974739 8 44 4.709602417321733 8 45 3.229017502308505 8 46 3.9213094160085653 8 47 4.18513541509801 8 48 3.49747173690427 8 49 6.97012237163743 8 50 4.368514329494502 8 51 4.482800981546429 8 52 4.217380022811515 8 53 4.898489183687648 8 54 5.847239487901408 8 55 5.163749692203602 8 56 6.341041611405309 9 1 1.2330783000920817 9 2 2.456948951734674 9 3 1.7997720229555227 9 4 2.932677354612885 9 5 2.166205580155406 9 6 3.7593403618117875 9 7 1.898676182487904 9 8 2.028770091868772 9 9 1.9752031072578973 9 10 2.3048364290243555 9 11 3.48770705916985 9 12 4.297640320214498 9 13 4.558625135770136 9 14 4.757000061770583 9 15 4.774754599430423 9 16 4.044585829149717 9 17 5.0997329893454975 9 18 5.1392781205345415 9 19 4.186554017449673 9 20 3.9630301674940327 9 21 2.8494864316385105 9 22 2.8283399907913918 9 23 3.393209328926872 9 24 3.6168399631775046 9 25 2.6466726503657583 9 26 2.347608076331724 9 27 2.440510632549362 9 28 2.3250287181463207 9 29 2.7752813494413786 9 30 1.9802998788572879 9 31 2.2546095512765802 9 32 2.1963644392324966 9 33 2.425149079233556 9 34 1.9381953776855312 9 35 2.198527571819163 9 36 3.170969203148651 9 37 3.550510476033118 9 38 3.9291272463455513 9 39 3.7157368377896134 9 40 6.797143947040091 9 41 4.681799021108773 9 42 5.421958251089098 9 43 7.485506364717342 9 44 7.173424470290399 9 45 5.655709557008176 9 46 6.164515232176125 9 47 5.714302803015613 9 48 5.129547208602386 9 49 8.25634359372502 9 50 5.536940848448978 9 51 5.356610917499342 9 52 5.707686410209821 9 53 4.578686517944181 9 54 6.637788215614751 9 55 5.472214293207582 9 56 6.242634133475587 10 1 1.5625122623562362 10 2 2.7810998951309003 10 3 1.9107154191819404 10 4 2.432111316877041 10 5 2.299790485815784 10 6 4.232736588226885 10 7 1.8720724089030014 10 8 1.764996506963115 10 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36 2.872267904447355 114 37 3.248378645599402 114 38 2.4830356021407027 114 39 3.0194614150990944 114 40 7.00665142069851 114 41 7.193438325200908 114 42 1.7800812601555052 114 43 -0.7118717288871714 114 44 -7.90388501267946 114 45 2.8235605739157954 114 46 -3.472828556111061 114 47 -3.0575668367785553 114 48 -3.1129178734853404 114 49 -17.835668168098064 114 50 -3.240564664905591 114 51 -4.850691214331093 114 52 -4.227991119807331 114 53 -5.4684832933765755 114 54 -11.561672700827295 114 55 -5.878190019460881 114 56 -1.857218843735879 115 1 0.6767227886720235 115 2 2.142152526709843 115 3 0.9770312086556245 115 4 2.415795527403354 115 5 1.2687378542368393 115 6 3.6895786934900414 115 7 1.0657566194293153 115 8 1.1092070332788992 115 9 1.0256003267216514 115 10 1.5511124965516672 115 11 -1.740445870323697 115 12 -11.192180931026812 115 13 -7.176479134339097 115 14 -9.243248200394266 115 15 -10.997340733240879 115 16 -10.080290039767863 115 17 -3.1716175568312615 115 18 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1.5944216210624802 116 1 1.128875898719869 116 2 2.3801908042218045 116 3 1.7007154191819396 116 4 2.703474953240672 116 5 1.792063213088511 116 6 4.327736588226884 116 7 1.6147996816302737 116 8 1.4931783251449318 116 9 1.3467725755254847 116 10 1.4392703912885096 116 11 -2.6896085497495363 116 12 -13.051630691792365 116 13 -8.672292531468283 116 14 -4.3204013104421115 116 15 -6.511694800226525 116 16 -4.172395302925757 116 17 -2.3408759300370043 116 18 -8.252016905194441 116 19 -2.9510960683136176 116 20 -4.156395218440785 116 21 2.0100781983623506 116 22 2.586058687189336 116 23 2.3181664472802197 116 24 1.7718571158361627 116 25 1.494442804739684 116 26 0.9228653662116599 116 27 0.9758879910399259 116 28 0.3368211709765063 116 29 0.791576375170365 116 30 1.5274696901780445 116 31 0.5775083505904774 116 32 1.1088858800558228 116 33 1.1176876727155474 116 34 0.13609417699942838 116 35 0.7362634208757655 116 36 1.2618782940577447 116 37 1.3538981261188852 116 38 1.552840796945894 116 39 2.688292583930263 116 40 5.742041031088121 116 41 3.8346720914346726 116 42 -2.6468667917925472 116 43 -8.482975624991067 116 44 -9.470443454237902 116 45 -3.195919945564725 116 46 -6.01665972494223 116 47 -5.091657745869462 116 48 -5.241164626732093 116 49 -7.718785051214947 116 50 -4.699980249321175 116 51 -2.9107561493960254 116 52 -3.500718392534605 116 53 -3.661665111558392 116 54 -7.952906467061062 116 55 -3.473969240240102 116 56 -2.488712350229388 117 1 0.48097380081777175 117 2 1.9134075874385914 117 3 1.0353308037973221 117 4 1.8805728553385777 117 5 1.339790485815783 117 6 3.4335058189961174 117 7 0.6959185627491564 117 8 0.9942272761938824 117 9 0.950094253847162 117 10 1.1296550066731257 117 11 -2.7932798784208615 117 12 -7.594812509974183 117 13 -10.886803020978775 117 14 -10.132114597155397 117 15 -9.694911583443307 117 16 -14.857010687541141 117 17 -15.056435370596443 117 18 -14.228065856243393 117 19 -6.804592571810122 117 20 -6.654821791867359 117 21 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0.7657839123326227 118 4 1.9147140566030671 118 5 0.8986945954048267 118 6 3.3945174101446938 118 7 0.72563405273862 118 8 0.6167773288809215 118 9 0.6137401969451588 118 10 0.7115306652611153 118 11 -1.9749447888528948 118 12 -8.638648126412537 118 13 -8.03390523383441 118 14 -10.370681509694911 118 15 -6.164247726752052 118 16 -13.028559686487402 118 17 -11.730197225180216 118 18 -12.33280768975235 118 19 -6.083412382136782 118 20 -5.762092603247758 118 21 -0.41660299715445603 118 22 0.3590972924197189 118 23 0.4168588507671416 118 24 0.3977911133455052 118 25 -0.04136043311834925 118 26 1.2536561507695652 118 27 0.9639016896700596 118 28 1.4857937737162317 118 29 1.445997296714573 118 30 1.3356888682602346 118 31 0.07781968309358689 118 32 -4.229843883331476 118 33 0.47852204382389374 118 34 0.740857564297059 118 35 -0.01031192158998806 118 36 1.1024760524637216 118 37 0.9082256479121611 118 38 1.9605307097727973 118 39 1.337732185424663 118 40 6.070341155621122 118 41 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1.3214216617046262 120 5 0.4915146237468164 120 6 2.6854952089165423 120 7 0.29034827097196825 120 8 0.42499650696311164 120 9 0.225691070823288 120 10 0.5556497016333353 120 11 -3.4595928757683403 120 12 -11.805157337560388 120 13 -10.617041747769225 120 14 -13.067658363733646 120 15 -12.792259063549409 120 16 -17.517222889132654 120 17 -15.269167466087161 120 18 -16.90366267321952 120 19 -10.008306099661581 120 20 -15.14659898019627 120 21 -6.5639813627661745 120 22 -4.605947582403143 120 23 -4.554090606011318 120 24 -10.462860752502394 120 25 -3.8887860353857064 120 26 0.7038214790643238 120 27 0.44571557724682265 120 28 -0.06404089798900969 120 29 0.09408421216095775 120 30 0.25160762121252844 120 31 0.1472575668914189 120 32 -14.881709731229446 120 33 -0.7671085655289644 120 34 -1.1174324687685981 120 35 -0.7982193377449249 120 36 -0.8462721761616905 120 37 -0.112794663849769 120 38 -0.6795259742139805 120 39 -0.9010491088597057 120 40 3.7236241031884347 120 41 -1.8491053380324125 120 42 -5.701333876432045 120 43 -14.14559317985 120 44 -13.931650350789626 120 45 -9.72099831547068 120 46 -16.867365054095835 120 47 -15.80521573959987 120 48 -13.1059765389578 120 49 -18.585556211089553 120 50 -13.622785891954404 120 51 -11.644690318675021 120 52 -12.480342216986017 120 53 -12.537448810617954 120 54 -15.280884523487394 120 55 -11.787057014534772 120 56 -10.026721754618102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 600.0 5 68.0 6 39.0 7 76.0 8 99.0 9 113.0 10 85.0 11 53.0 12 81.0 13 78.0 14 121.0 15 204.0 16 276.0 17 348.0 18 417.0 19 487.0 20 625.0 21 775.0 22 1013.0 23 1257.0 24 1480.0 25 1575.0 26 1785.0 27 2027.0 28 2321.0 29 2786.0 30 3260.0 31 3606.0 32 4246.0 33 4964.0 34 5736.0 35 6837.0 36 8558.0 37 10614.0 38 10017.0 39 5573.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.48787193148918 0.3637204097024548 0.12164562197406516 0.02676203683429433 8 99.01467046201007 0.7736661557550544 0.1398924652701749 0.07177091696469844 9 97.06955696664477 1.8721261221808627 0.5194268058292582 0.5388901053451086 10 35.58134442741406 42.19643335036372 13.197333527966329 9.024888694255893 11 28.257061528355592 36.19078899350413 19.615356543318004 15.936792934822275 12 34.88918083838163 17.210422596890737 20.138432717806488 27.76196384692115 13 24.474673610181657 18.49075887914147 23.799384330855244 33.235183179821625 14 24.216877729523254 21.623298419765703 28.365144824398136 25.794679026312906 15 28.313480693968618 21.165311322710036 26.20914156130747 24.312066422013874 16 30.02239205568807 22.681692060555907 25.19836440636713 22.09755147738889 17 28.90299978103788 22.823151594774103 25.76332627788726 22.510522346300757 18 24.098290858220302 25.246639498813938 27.402226141962167 23.25284350100359 19 28.393483343074227 26.780148061562436 22.15565945840639 22.670709136956944 20 25.03653546375682 28.674249805144193 22.99542088854248 23.29379384255651 21 24.825438532467217 22.466729922390094 30.190010461523492 22.5178210836192 22 24.885044887234507 23.197820110454227 24.768265090139405 27.148869912171865 23 25.27917670243048 25.286475439748923 25.208622241685525 24.225725616135072 24 23.758606427754664 24.764615721480183 29.894411600126514 21.58236625063864 25 27.550970755680986 24.65695099995134 27.778453603230986 20.013624641136683 26 22.717485188744664 22.077590296955023 30.72833663824041 24.476587876059902 27 22.05338008961621 21.09146697837522 33.72175141242938 23.133401519579195 28 20.824046670807604 25.02831670868501 35.484185270439795 18.663451350067596 29 24.35898996045026 21.539397627015518 36.34317006388804 17.75844234864618 30 19.534034916965755 18.965873836608065 43.75691952065211 17.743171725774072 31 17.261687474142757 22.914993550899222 42.24891095373683 17.574408021221192 32 23.763774733637746 18.380517503805173 42.08706240487062 15.768645357686454 33 18.282609225370987 19.526547917834254 44.212821949852575 17.978020906942177 34 15.593282219788243 22.29402458318121 47.366435438724594 14.74625775830595 35 16.83379171027332 19.621778178012512 50.876189549005765 12.6682405627084 36 17.81607097221715 22.677765202682146 46.532315967531915 12.973847857568789 37 17.101350363184324 19.26583142397504 50.614244625359525 13.018573587481107 38 19.684972120886762 19.21279632996181 47.974036431961544 13.128195117189884 39 15.458112950008532 19.388451507543813 47.081190435566825 18.072245106880835 40 13.008966632368073 14.493605762163753 44.786613748836295 27.710813856631876 41 12.274222047707863 13.781044468440168 42.307597918916265 31.637135564935704 42 17.848354165143874 19.29805326121385 48.33832809492484 14.515264478717443 43 13.821108468995794 16.511188342174258 49.433571123711964 20.23413206511798 44 13.56497720634067 17.244961562435073 45.067892105938576 24.122169125285684 45 15.354421893094411 19.807399280794783 50.22734198817578 14.610836837935029 46 15.459896893395571 19.65363005935333 46.48206603210199 18.404407015149115 47 13.965317919075146 22.621235168846972 47.29662306054153 16.116823851536356 48 15.774338978920433 23.92226148409894 46.49567442427197 13.807725112708663 49 12.922982885085574 19.952322738386307 39.9278728606357 27.196821515892424 50 17.965647460104766 21.766354001705444 46.02387623340236 14.24412230478743 51 16.496241702459976 24.95607184693479 44.709098008590395 13.838588442014837 52 15.200389816055548 22.500913631380193 47.86332074552321 14.435375807041053 53 16.326058080161836 21.56253427411984 47.217246127786105 14.89416151793222 54 13.567918846092883 24.12182840142897 42.60214345806357 19.708109294414573 55 16.071167260133652 25.028047412321353 44.057606946002636 14.843178381542362 56 19.444207447132673 23.590712413919192 42.501066487902975 14.464013651045157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 2.0 6 3.0 7 4.0 8 22.0 9 40.0 10 103.0 11 166.0 12 263.5 13 495.0 14 629.0 15 688.5 16 748.0 17 966.5 18 1185.0 19 1307.0 20 1429.0 21 1732.0 22 2457.0 23 2879.0 24 3410.0 25 3941.0 26 4230.0 27 4519.0 28 4581.0 29 4537.5 30 4432.0 31 3970.5 32 3509.0 33 2931.0 34 2353.0 35 2110.0 36 1867.0 37 1719.5 38 1590.5 39 1609.0 40 1621.5 41 1634.0 42 1781.5 43 1929.0 44 2542.5 45 3156.0 46 3345.0 47 3213.5 48 2893.0 49 2893.0 50 2893.0 51 2820.0 52 2747.0 53 2721.5 54 2774.0 55 2852.0 56 2917.5 57 2983.0 58 3119.0 59 3255.0 60 3209.0 61 3163.0 62 2935.0 63 2477.0 64 2247.0 65 1985.0 66 1723.0 67 1529.0 68 1335.0 69 1141.0 70 947.0 71 793.5 72 532.0 73 424.0 74 339.0 75 254.0 76 195.0 77 136.0 78 108.5 79 58.0 80 35.0 81 24.0 82 13.0 83 8.5 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02311266817507238 14 0.0036493686592219544 15 0.22504440065202053 16 0.04135951147118215 17 0.0 18 0.0012164562197406515 19 0.09488358513977081 20 0.11434688465562125 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002432912439481303 26 0.0060822810987032575 27 0.09488358513977081 28 0.1204291657543245 29 0.037710142811960196 30 0.013381018417147166 31 0.029194949273775635 32 0.09853295379899278 33 0.1557063961268034 34 0.04379242391066346 35 0.03892659903170085 36 0.0401430552514415 37 0.18733425784006033 38 0.2980317738364596 39 0.18490134540057904 40 0.6933800452521715 41 0.8636839160158626 42 0.14597474636887817 43 0.24450770016787096 44 0.46833564460015087 45 0.20801401357565139 46 0.18855071405980098 47 0.037710142811960196 48 0.1654380458847286 49 0.4938812252147045 50 0.14110892148991558 51 0.3089798798141255 52 0.14110892148991558 53 0.17881906430187577 54 0.22991022553098314 55 0.2457241563876116 56 0.19584945137824492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 82206.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.14908887429141 #Duplication Level Percentage of deduplicated Percentage of total 1 86.82962841440407 64.38337834221346 2 7.751620047576082 11.495511276549157 3 2.4214584529570997 5.386468141011605 4 1.095890410958904 3.2503710191470208 5 0.5643507505536872 2.092304697953921 6 0.3428758920515134 1.525436099554777 7 0.24444262160610283 1.2687638371894996 8 0.1607743417275039 0.9537016762766708 9 0.1082765974899516 0.722574994525947 >10 0.45279304404888854 5.3901175096708265 >50 0.019686654089082108 1.0729143858112546 >100 0.006562218029694037 1.1994258326642824 >500 0.0 0.0 >1k 0.0016405545074235093 1.2590321874315742 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1035 1.2590321874315742 No Hit TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 307 0.37345205946038 No Hit TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 276 0.33574191664841985 No Hit TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 260 0.3162786171325694 No Hit TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 143 0.1739532394229132 No Hit TAGAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 98 0.11921270953458385 No Hit TAGAGAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG 94 0.11434688465562125 No Hit TAGAGAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 87 0.10583169111743668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.012164562197406515 0.0 0.0 0.0 0.0 5 0.012164562197406515 0.0 0.0 0.0 0.0 6 0.03041140549351629 0.0 0.0 0.0 0.0 7 0.03162786171325694 0.0 0.0 0.0 0.0 8 0.03162786171325694 0.0 0.0 0.0 0.0 9 0.03162786171325694 0.0 0.0 0.0 0.0 10 0.045008880130404105 0.0 0.0 0.0 0.0 11 0.05352407366858867 0.0 0.0 0.0 0.0 12 0.057173442327810624 0.0 0.0 0.0 0.0 13 0.059606354767291925 0.0 0.0 0.0 0.0 14 0.06690509208573583 0.0 0.0 0.0 0.0 15 0.07420382940417974 0.0 0.0 0.0 0.0 16 0.0778531980634017 0.0 0.0 0.0 0.0 17 0.080286110502883 0.0 0.0 0.0 0.0 18 0.09001776026080821 0.0 0.0 0.0 0.0 19 0.09731649757925212 0.0 0.0 0.0 0.0 20 0.09974941001873343 0.0 0.0 0.0 0.0 21 0.10461523489769603 0.0 0.0 0.0 0.0 22 0.11313042843588059 0.0 0.0 0.0 0.0 23 0.12894435929250905 0.0 0.0 0.0 0.0 24 0.13624309661095296 0.0 0.0 0.0 0.0 25 0.14719120258861884 0.0 0.0 0.0 0.0 26 0.1557063961268034 0.0 0.0 0.0 0.0 27 0.16908741454395057 0.0 0.0 0.0 0.0 28 0.18125197674135707 0.0 0.0 0.0 0.0 29 0.18490134540057904 0.0 0.0 0.0 0.0 30 0.1934165389387636 0.0 0.0 0.0 0.0 31 0.1958494513782449 0.0 0.0 0.0 0.0 32 0.19949882003746686 0.0 0.0 0.0 0.0 33 0.20193173247694815 0.0 0.0 0.0 0.0 34 0.20193173247694815 0.0 0.0 0.0 0.0 35 0.20679755735591077 0.0 0.0 0.0 0.0 36 0.21287983845461403 0.0 0.0 0.0 0.0 37 0.21287983845461403 0.0 0.0 0.0 0.0 38 0.21409629467435468 0.0 0.0 0.0 0.0 39 0.21409629467435468 0.0 0.0 0.0 0.0 40 0.21896211955331726 0.0 0.0 0.0 0.0 41 0.21896211955331726 0.0 0.0 0.0 0.0 42 0.22139503199279859 0.0 0.0 0.0 0.0 43 0.22747731309150185 0.0 0.0 0.0 0.0 44 0.23234313797046444 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGTAC 15 0.0080547 50.538273 38 GCGTAGA 25 1.9431147E-5 50.538273 38 GCCTGTC 55 0.0 50.53827 38 CTGTACC 15 0.008074388 50.507095 39 AGCGTAG 30 9.702544E-7 50.475956 37 TGCTCGT 55 0.0 50.475956 37 TAAAGGC 15 0.008213215 50.289925 34 CGATTCA 20 4.1055508E-4 50.13595 49 ACGATTC 20 4.1055508E-4 50.13595 48 CGATTAA 25 2.0373354E-5 50.135944 49 ACGATTA 25 2.0373354E-5 50.135944 48 CAACGAT 40 2.581146E-9 50.105263 46 TCGCACT 15 0.008353808 50.07462 42 ACCCGAC 15 0.008353808 50.07462 42 GGTCCTA 15 0.0084146075 49.982906 50 GATTCAG 30 1.0383337E-6 49.982906 50 CTTAATC 30 1.0427211E-6 49.95241 15 GACAGTC 15 0.008434947 49.95241 9 GATGTAC 15 0.008434947 49.95241 9 GCATTAT 30 1.0427211E-6 49.95241 12 >>END_MODULE