FastQCFastQC Report
Fri 17 Jun 2016
SRR1526205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44450
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1650.3712035995500562No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC860.19347581552305962No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC810.18222722159730034No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC650.14623172103487064No Hit
CCAGACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG640.14398200224971877No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC550.12373453318335209No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG490.11023622047244094No Hit
CCAGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG490.11023622047244094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCAT150.00808057950.4583838
GCTTGTC451.1823431E-1050.4583838
GCATGAG150.00808057950.4583840
CTTGTCT451.1823431E-1050.4583839
CGTTGTA203.9574096E-450.4583839
GTTGTAG203.9574096E-450.4583840
TGCTTGT451.200533E-1050.40091337
TATGCTT505.456968E-1250.22928635
ATGCTTG550.050.22928636
GCCTTGT150.00822748850.22928245
AACGATT150.00822748850.22928247
TCAAAGA402.4792826E-950.22928245
AAGATTA402.4792826E-950.22928248
CGCAGGC150.00822748850.22928235
CAAAGAT402.4792826E-950.22928246
CCCCCTG150.00822748850.22928249
AGATTAA402.4792826E-950.22928249
AAAGATT402.4792826E-950.22928247
CCAAAAC150.00826452450.17233743
GATTAAG505.456968E-1250.17233750