FastQCFastQC Report
Fri 17 Jun 2016
SRR1526204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61736
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12321.9955941428016069No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3700.5993261630167164No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3280.5312945445121161No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2790.4519243229234159No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG950.15388104185564339No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG790.12796423480627186No Hit
GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC770.12472463392510043No Hit
GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA760.1231048334845147No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1133860308410004No Hit
GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA700.1133860308410004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC203.9804744E-450.4305738
AAACACC150.0081132550.4305738
GTCTGGC150.00813966550.38916437
ATGCCTG251.990798E-550.30655736
ATGCCCG150.00819268650.30655736
TATGCTA204.0455523E-450.26535835
GTGAAGG150.00821929250.26535834
CTACCCG150.00821929250.26535834
TGCCAAG150.00824596450.22422447
CAACGAT550.050.22422446
AACGATT507.2759576E-1250.2242247
ACGATTA507.2759576E-1250.2242248
CGATTAA507.2759576E-1250.2242249
TAGCGCC150.00827269950.18315544
CCAAAAC355.032598E-850.18315543
CACAATT150.00835329250.0603642
GCAGCAT301.0230287E-650.0603642
TGTAGCG150.00835329250.0603642
TTCGGCG150.00835329250.0603633
TGCTACC150.00840734449.9788332