##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526204_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 61736 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.91428016068421 39.0 38.0 40.0 35.0 40.0 2 36.93901451341195 39.0 37.0 39.0 33.0 40.0 3 37.60107554749255 39.0 38.0 40.0 35.0 40.0 4 37.215838408708045 39.0 37.0 39.0 33.0 40.0 5 35.96198328365945 38.0 35.0 39.0 30.0 40.0 6 37.35799209537385 39.0 38.0 40.0 33.0 40.0 7 37.68663340676429 39.0 38.0 40.0 35.0 40.0 8 36.76985875340158 39.0 35.0 40.0 31.0 40.0 9 36.76559867824284 39.0 35.0 40.0 31.0 40.0 10 36.34362446546586 39.0 35.0 39.0 31.0 40.0 11 35.673804587274844 38.0 35.0 39.0 28.0 40.0 12 34.25660878579759 38.0 33.0 39.0 22.0 40.0 13 34.16308150835817 38.0 33.0 39.0 22.0 40.0 14 34.16368083452119 38.0 33.0 39.0 22.0 40.0 15 34.14947518465725 38.0 33.0 39.0 22.0 40.0 16 33.53589477776338 38.0 31.0 39.0 21.0 40.0 17 33.581864714267205 38.0 31.0 39.0 21.0 40.0 18 33.06190877283919 37.0 30.0 39.0 19.0 40.0 19 33.939111701438385 37.0 31.0 39.0 23.0 40.0 20 33.89720746404043 37.0 31.0 39.0 23.0 40.0 21 34.71932097965531 38.0 33.0 39.0 25.0 40.0 22 34.55040818971103 38.0 33.0 39.0 24.0 40.0 23 34.95433782557989 38.0 33.0 39.0 26.0 40.0 24 34.52557664895685 38.0 33.0 39.0 24.0 40.0 25 34.78293054295711 38.0 33.0 39.0 26.0 40.0 26 35.29891797330569 38.0 35.0 39.0 28.0 40.0 27 35.26359012569652 38.0 35.0 39.0 27.0 40.0 28 35.1307988855773 38.0 35.0 39.0 27.0 40.0 29 34.926072307891666 38.0 34.0 39.0 27.0 40.0 30 34.78778994427886 38.0 34.0 39.0 26.0 40.0 31 35.15716923675003 38.0 35.0 39.0 28.0 40.0 32 34.891748736555655 38.0 35.0 39.0 27.0 40.0 33 35.09297654528962 38.0 35.0 39.0 28.0 40.0 34 35.01056109887262 38.0 35.0 39.0 28.0 40.0 35 34.87995658934819 38.0 35.0 39.0 27.0 40.0 36 34.23030322664248 37.0 33.0 39.0 27.0 40.0 37 33.980902552805496 37.0 33.0 39.0 26.0 40.0 38 33.74642024102631 36.0 33.0 39.0 26.0 40.0 39 33.2295419204354 36.0 32.0 39.0 23.0 40.0 40 29.9131624983802 35.0 25.0 38.0 9.0 40.0 41 26.506576389788776 32.0 15.0 38.0 5.0 39.0 42 30.814127899442788 35.0 29.0 38.0 11.0 39.0 43 29.329111053518208 34.0 26.0 38.0 5.0 39.0 44 29.37281326940521 34.0 26.0 38.0 4.0 39.0 45 30.475686795386807 35.0 29.0 38.0 4.0 39.0 46 30.160959569781003 35.0 28.0 38.0 4.0 40.0 47 29.71457496436439 35.0 27.0 38.0 4.0 40.0 48 29.727776337955163 35.0 27.0 38.0 4.0 40.0 49 27.28241220681612 33.0 17.0 38.0 4.0 39.0 50 29.354606712453027 35.0 26.0 38.0 4.0 40.0 51 29.571562783465076 35.0 27.0 39.0 4.0 40.0 52 29.38207852792536 35.0 27.0 39.0 4.0 40.0 53 28.97188026435143 35.0 25.0 39.0 4.0 40.0 54 28.08367889076066 35.0 21.0 39.0 4.0 40.0 55 28.31130944667617 35.0 23.0 39.0 4.0 40.0 56 27.3133341972269 34.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.15483178247252738 1 2 0.7281934960775232 1 3 0.5648174138365434 1 4 0.9859650097739561 1 5 1.759237662404388 1 6 0.7133841076612413 1 7 0.8726678873066547 1 8 0.6309997249934653 1 9 0.8164932160033871 1 10 0.5922166276814664 1 11 -5.1036751433505145 1 12 -16.22104615107424 1 13 -10.221000917035052 1 14 -7.859874072662123 1 15 -8.214167310627555 1 16 -22.701843237923974 1 17 -5.01502501181 1 18 -11.917095879293317 1 19 -5.691630976160766 1 20 -5.953883451383437 1 21 -5.147537508365861 1 22 -3.0170059207875823 1 23 -3.526542443584855 1 24 -4.124318153703403 1 25 -3.0222081335503717 1 26 2.0670305381045324 1 27 2.2621015368153863 1 28 2.094402940080606 1 29 1.2395600221039729 1 30 2.0715038344637193 1 31 1.555735136190897 1 32 1.8441331717550966 1 33 0.9707014241287766 1 34 1.518978595933305 1 35 1.3749405161652177 1 36 1.1479576759648609 1 37 1.4233201228747419 1 38 1.5225055246040142 1 39 1.8155067175872546 1 40 5.182415995779056 1 41 -11.457569858278688 1 42 -12.257524017165931 1 43 -18.164068242251112 1 44 -22.98002762012494 1 45 -13.837506414882782 1 46 -18.96902925776427 1 47 -23.205645104190367 1 48 -16.488048513042923 1 49 -20.73861369968443 1 50 -15.144215400659329 1 51 -12.606041731797305 1 52 -12.54370159029906 1 53 -12.266473520321654 1 54 -11.61873626517418 1 55 -12.13179428406469 1 56 -6.8811970185764935 2 1 1.0003560791477142 2 2 1.5996342462906554 2 3 1.366010934723164 2 4 1.4621798435335478 2 5 1.7394593162833303 2 6 1.4866151392042894 2 7 1.3205792257550826 2 8 1.7786553089661794 2 9 1.734992789745931 2 10 1.2859932687726854 2 11 0.9236053340578394 2 12 -2.869127992506467 2 13 -2.4194663901808333 2 14 2.5820696613532235 2 15 1.5974268922709953 2 16 1.5641414168074803 2 17 2.075853078556584 2 18 -2.127326058321451 2 19 1.3578148891418778 2 20 1.110225670526198 2 21 0.5992655606878472 2 22 1.412320592426397 2 23 0.2964754592284464 2 24 -0.8151962440699876 2 25 0.5488063592032546 2 26 0.9297756361437521 2 27 1.0532353816576716 2 28 1.1835760006091647 2 29 1.6011968507484724 2 30 1.6423478242335392 2 31 0.8479772504278955 2 32 0.8340734957107685 2 33 1.636345072892631 2 34 1.4710672574848758 2 35 0.33896438658295125 2 36 1.1273268149247926 2 37 1.2234906258585454 2 38 1.5970153285255861 2 39 2.3737334865557145 2 40 4.704837138149049 2 41 -8.18442407822754 2 42 -9.867924699177866 2 43 -15.384017091355972 2 44 -16.129729239903284 2 45 -8.647054581975706 2 46 -13.847119624345686 2 47 -18.175466076057376 2 48 -11.03945516265929 2 49 -14.610565958848964 2 50 -10.278401248911674 2 51 -5.889247187892785 2 52 -5.337222476914576 2 53 -5.562125694234698 2 54 -5.105863289897112 2 55 -5.5092878902027955 2 56 -2.345476643469926 3 1 1.203624053003928 3 2 1.5035558149181085 3 3 1.3836579935466915 3 4 1.7366896474551154 3 5 2.2263874208584937 3 6 1.5670072960670325 3 7 1.6369190950361272 3 8 2.2015311259596473 3 9 2.0140777570661896 3 10 2.037627255700791 3 11 2.619030170659144 3 12 4.304074621872623 3 13 2.830206812433545 3 14 2.8990631254055117 3 15 3.5974268922709953 3 16 3.844533573670226 3 17 5.016375954373579 3 18 5.1785562946197246 3 19 3.6349390721484127 3 20 3.542251814317048 3 21 3.5365204626486317 3 22 2.911013402883917 3 23 3.3749068317774658 3 24 3.733169769001911 3 25 3.1599174703143618 3 26 2.400363871437868 3 27 2.753888976428918 3 28 2.486190379694129 3 29 2.8704778965001125 3 30 2.4096680856714485 3 31 1.738826923630505 3 32 2.794857809436259 3 33 2.0069333081867455 3 34 1.7991718326482768 3 35 2.0657617722038637 3 36 2.484189560022834 3 37 2.2561703644206403 3 38 3.6225055246040156 3 39 3.7671975388432983 3 40 7.268889425730745 3 41 8.05740598713194 3 42 5.216389026312331 3 43 -1.7964353920095668 3 44 1.488571413691485 3 45 4.33399116965828 3 46 3.818893447549737 3 47 -2.035596795011621 3 48 3.2520481053145645 3 49 5.665251034615089 3 50 4.002644502722315 3 51 5.785262616028785 3 52 6.026829810667124 3 53 5.237874305765306 3 54 6.705901415985242 3 55 6.2266598222155025 3 56 5.334261918621575 4 1 1.1874404781246994 4 2 1.5723963946282495 4 3 1.5140927761553868 4 4 1.485965009773956 4 5 2.469382589940622 4 6 1.4162826583858745 4 7 1.4451316554225997 4 8 2.1165069713702778 4 9 1.9433048102062855 4 10 1.9110572073916146 4 11 2.7803828276639777 4 12 5.050692979360541 4 13 4.3007382133997325 4 14 4.031430275163963 4 15 4.151774718357949 4 16 4.5264176316412446 4 17 5.263960495436379 4 18 4.408991077228421 4 19 4.533006704998655 4 20 4.158435389196271 4 21 3.5915929264167445 4 22 4.247486832835605 4 23 3.027805382502102 4 24 3.9662615564415233 4 25 2.9053280983336904 4 26 3.302537784481345 4 27 2.81282617449655 4 28 2.9023739545733562 4 29 3.5330382829735356 4 30 3.006286443159368 4 31 2.5810974550314754 4 32 2.5542780992913308 4 33 2.311281134273699 4 34 1.9899930886869228 4 35 2.621317327759421 4 36 3.8073779658199385 4 37 3.557378093889241 4 38 3.8667084231547406 4 39 3.9278255581669654 4 40 6.2838652711413765 4 41 10.151125793895222 4 42 5.684504968341312 4 43 7.248975236009759 4 44 6.545334698715642 4 45 5.253073295262144 4 46 5.0889417567284845 4 47 4.794354895809633 4 48 5.685864530435339 4 49 6.960661662634415 4 50 5.30868315006531 4 51 4.6149727609563165 4 52 4.82224043868645 4 53 5.23352647967835 4 54 5.85590141598524 4 55 4.889944846370092 4 56 6.155034865481483 5 1 0.5194205724149015 5 2 1.3810658550787522 5 3 1.449923053787657 5 4 1.1792599285796186 5 5 1.265610981768802 5 6 1.4951197458662335 5 7 0.9833715716090836 5 8 1.6699380871376945 5 9 1.336835454522685 5 10 1.7334773226351956 5 11 2.7641439591464305 5 12 4.491892560292968 5 13 4.1613982448297975 5 14 4.2590363516438 5 15 4.440800386246899 5 16 4.247344818650149 5 17 4.4293612288046305 5 18 4.947230993414905 5 19 4.958098376030605 5 20 3.766214331050648 5 21 3.5638296995964183 5 22 3.1839719035532568 5 23 2.7516136590867006 5 24 3.90117512375425 5 25 2.3463967206490395 5 26 1.2316891726426888 5 27 2.07945791886533 5 28 1.7029239807650782 5 29 2.8723520598200523 5 30 2.179413734935167 5 31 1.4591749825327867 5 32 1.5595164439744096 5 33 0.8081381274638559 5 34 0.09502189958268303 5 35 1.115293231373876 5 36 2.133988756809984 5 37 2.0695572452774016 5 38 2.6871641591421707 5 39 3.4633153433948323 5 40 5.4818747001618 5 41 8.826348423544257 5 42 4.454541636754097 5 43 5.997272773987756 5 44 5.623778910344761 5 45 4.3226123209300376 5 46 4.994127717964734 5 47 4.455433994814349 5 48 4.231700046412286 5 49 7.151730284949764 5 50 4.44789216001816 5 51 3.7531341019725595 5 52 3.531381350158423 5 53 4.001729727452052 5 54 4.250479729238251 5 55 3.9787347887483016 5 56 3.342829522369904 6 1 1.2369573863372594 6 2 0.917136061831684 6 3 1.2293370058923685 6 4 1.4873069314057332 6 5 2.2992269270313344 6 6 1.66453816026457 6 7 1.5430919345422964 6 8 1.654617545712732 6 9 1.6721024484242193 6 10 1.0672568853304227 6 11 2.4239684422640835 6 12 4.191728942860273 6 13 3.8079845902113263 6 14 4.031161890837609 6 15 4.313476274987046 6 16 4.7667557958924505 6 17 5.220079658077282 6 18 5.2476920970888585 6 19 4.205309442518782 6 20 3.664474036539268 6 21 3.3291130552412227 6 22 3.1171862423900834 6 23 2.8218204120243797 6 24 3.368972238137715 6 25 3.2697940135242405 6 26 2.116413254153919 6 27 2.168703791243729 6 28 2.186190379694132 6 29 2.613687773043317 6 30 2.705964381967746 6 31 2.372160256963838 6 32 2.493623241535019 6 33 2.9143407155941503 6 34 1.8485545486976633 6 35 1.7818111549199145 6 36 3.647152522985799 6 37 3.414195055778663 6 38 3.929912932011426 6 39 4.0141111190902095 6 40 7.223210413385065 6 41 5.257405987131939 6 42 6.459598902855536 6 43 7.150478188237347 6 44 6.599682524802596 6 45 6.241398577065684 6 46 6.75346134878431 6 47 7.240946414864922 6 48 6.627356747289873 6 49 9.939325108689161 6 50 7.839681539759351 6 51 7.4642749617077975 6 52 7.455224872395519 6 53 7.482318750209746 6 54 8.488617465367955 6 55 6.750116612338964 6 56 7.694755745782068 7 1 0.9966796085594822 7 2 1.65772248158477 7 3 1.3961579935466943 7 4 0.953356314121784 7 5 1.7541651986362723 7 6 0.971173962733701 7 7 1.214696872813903 7 8 1.4279200148485387 7 9 1.8335222015106396 7 10 0.8029050334785666 7 11 2.32875239288137 7 12 4.223519066317067 7 13 3.9246512568779934 7 14 3.9129520142943974 7 15 4.247426892270994 7 16 4.1987002403368905 7 17 4.717764843262465 7 18 5.4285562946197246 7 19 4.133550183259523 7 20 3.5491962587614907 7 21 2.957353795981966 7 22 3.7387911806616927 7 23 2.5707401651107986 7 24 3.0803919912241327 7 25 2.1238063592032503 7 26 2.275363871437868 7 27 0.7275000875400295 7 28 2.5181348241385777 7 29 2.273255674277884 7 30 1.7791125301158885 7 31 1.983271368074952 7 32 1.715691142769586 7 33 2.1444333081867484 7 34 1.4644496104260583 7 35 2.2213173277594223 7 36 1.425856226689504 7 37 1.3492259199761918 7 38 0.9808388579373499 7 39 1.8060864277321826 7 40 5.320278314619632 7 41 -0.3564829017569515 7 42 3.1372223596456656 7 43 4.94245349687932 7 44 4.062182524802594 7 45 3.736768947436058 7 46 3.3536156697719584 7 47 3.854680982766155 7 48 4.170103660870119 7 49 5.566639923503978 7 50 3.8054222805000926 7 51 3.9144292826954477 7 52 4.128218699556015 7 53 4.350374305765303 7 54 5.143401415985242 7 55 3.137770933326614 7 56 4.352317474177131 8 1 1.0541163174202381 8 2 1.0119946334835035 8 3 1.4532782467112497 8 4 1.026141124248369 8 5 1.7396082366109553 8 6 1.2222815576704136 8 7 1.0045702905354261 8 8 1.5372554578865092 8 9 1.4673829610043043 8 10 0.9369240208203422 8 11 2.5222650511092155 8 12 4.534595015684154 8 13 3.677182902447612 8 14 4.5006102421425 8 15 4.1670471454355535 8 16 4.312466063121704 8 17 4.379948387566266 8 18 4.981087940189347 8 19 3.9587084111076223 8 20 3.4310000562298413 8 21 2.474917087121206 8 22 2.7685380161047277 8 23 2.363936367642445 8 24 3.328809712743123 8 25 2.163996232620974 8 26 1.375047415741669 8 27 2.034620340704585 8 28 2.023039887429711 8 29 1.6710404844044717 8 30 1.107277087077918 8 31 0.9896004819990054 8 32 1.2345202566936422 8 33 1.494275080338646 8 34 0.9820129015652981 8 35 2.3934692264936004 8 36 1.2749068596008968 8 37 1.4851436414951849 8 38 1.4254591111019081 8 39 2.7320357948207885 8 40 5.078031479176598 8 41 -3.3530018890987208 8 42 2.7813679292659153 8 43 5.298782610803375 8 44 4.397150879232974 8 45 3.105439833512005 8 46 4.285419467240313 8 47 4.142180982766156 8 48 4.043046698844805 8 49 6.640215872871067 8 50 3.466498229867181 8 51 5.160790042189117 8 52 4.805592117277534 8 53 6.164456584246313 8 54 6.435015340035871 8 55 5.244100047250669 8 56 6.241716208354347 9 1 1.2124263618062372 9 2 2.0338588452211397 9 3 1.8057359156246164 9 4 1.4890705998360687 9 5 2.7872820817531547 9 6 1.7700375990973356 9 7 1.3082033663203987 9 8 1.9982122226407455 9 9 1.875405318393753 9 10 1.773846591920126 9 11 3.365927717556694 9 12 5.064103481901476 9 13 4.825300607527339 9 14 4.526913053255441 9 15 4.51950481434892 9 16 5.315096344232998 9 17 5.742927180924802 9 18 5.362971879035314 9 19 4.816180053389395 9 20 4.393189765254995 9 21 3.952970679098847 9 22 4.038141830012343 9 23 3.973175230045861 9 24 4.286560822392964 9 25 3.870884281281178 9 26 3.02374049481449 9 27 2.8026624252023637 9 28 3.3838815773853312 9 29 3.146956972979183 9 30 2.983982659986019 9 31 2.626128510932098 9 32 2.6113080258864727 9 33 2.2471930484464835 9 34 1.005521038997486 9 35 2.621317327759421 9 36 2.675531551364827 9 37 3.209128517378794 9 38 3.310384312482803 9 39 3.1625799342256897 9 40 6.159077015918339 9 41 2.3165690462949975 9 42 4.145826255749558 9 43 6.091154795580618 9 44 5.313968239088311 9 45 4.923457259124373 9 46 5.546310474966763 9 47 4.99932383990901 9 48 5.363447816714277 9 49 7.819074988439041 9 50 5.424578124655934 9 51 4.5817994125655765 9 52 4.9144199982573085 9 53 4.985926253817251 9 54 6.079927390011218 9 55 5.1533553489110275 9 56 5.037869422229079 10 1 0.7223375032963233 10 2 1.341275113163718 10 3 0.9231316777572189 10 4 1.3349352614902017 10 5 1.9436388828467983 10 6 0.896831857470545 10 7 1.5120652938665344 10 8 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115 29 2.2416982972287087 115 30 2.03444039896835 115 31 1.6472057943044618 115 32 0.3511419624417229 115 33 0.949556259006421 115 34 1.8556381350162212 115 35 1.7524648687430258 115 36 2.777085734886221 115 37 3.037955428172914 115 38 3.0197732841668596 115 39 3.4593651162567767 115 40 7.408597986750781 115 41 6.696385950702069 115 42 2.5611977694817263 115 43 -6.02721863426822 115 44 -7.564251901426914 115 45 2.775703373665568 115 46 -2.603146625310007 115 47 -1.693884591004334 115 48 -0.6776422407692237 115 49 -13.458974830594382 115 50 -1.9113809981884344 115 51 0.3863555122036466 115 52 -0.203953431591529 115 53 -1.5358961860379772 115 54 -7.008852682375416 115 55 -1.6720651322471554 115 56 1.3357191135213924 116 1 0.7831232705313127 116 2 1.754729523838293 116 3 1.3343622188988036 116 4 1.4146239197555843 116 5 2.6083905507489504 116 6 1.246350019071734 116 7 1.0682179995744647 116 8 1.3538002965386795 116 9 1.251479947989509 116 10 1.0463909489715277 116 11 -2.9587476071186316 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52 -10.160143369409504 118 53 -9.944884314924352 118 54 -13.842374446083726 118 55 -9.831194583914765 118 56 -6.634320456857353 119 1 0.02058311733141238 119 2 0.8982926570233687 119 3 0.35734220407300654 119 4 0.6594966649989757 119 5 1.8690774793380243 119 6 0.519638875014401 119 7 0.3761003815858359 119 8 0.15884106748011817 119 9 -0.5046356932262057 119 10 0.5136506475136571 119 11 -5.6605019930835425 119 12 -10.176042337191706 119 13 -13.297278567683414 119 14 -10.108539213775774 119 15 -10.507836265623741 119 16 -15.823887478961353 119 17 -14.717542174281395 119 18 -15.782847214152206 119 19 -6.825879641301881 119 20 -7.902777425449038 119 21 -0.33365497594785865 119 22 0.7747560929423898 119 23 0.838064726514304 119 24 0.823813043855715 119 25 0.6330168855190408 119 26 1.8389603626659365 119 27 0.6755264033294992 119 28 1.05168745571752 119 29 1.2166767269094692 119 30 1.6962177932737887 119 31 -0.8676058249075069 119 32 -6.219177278283041 119 33 -0.580785990058871 119 34 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-9.620662279600552 120 54 -10.747757120600127 120 55 -9.798814432527042 120 56 -6.837011794115551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 224.0 5 17.0 6 76.0 7 49.0 8 87.0 9 131.0 10 97.0 11 87.0 12 82.0 13 78.0 14 127.0 15 154.0 16 202.0 17 234.0 18 319.0 19 400.0 20 473.0 21 575.0 22 773.0 23 881.0 24 1050.0 25 1195.0 26 1302.0 27 1414.0 28 1565.0 29 2063.0 30 2330.0 31 2793.0 32 3252.0 33 3606.0 34 4297.0 35 5042.0 36 6449.0 37 7941.0 38 7549.0 39 4817.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.24355319424647 0.6220033691849165 0.08422962291045744 0.050213813658157316 8 98.58591421536866 1.0820266943112609 0.1959958533108721 0.13606323700920048 9 95.57146559543864 2.57710250097188 1.0026564727225606 0.8487754308669172 10 31.200596086562136 43.67305947907218 15.260139950758067 9.86620448360762 11 26.103084100038878 36.97194505636905 19.45704289231567 17.4679279512764 12 33.1897110276014 18.648762472463392 19.58824672800311 28.573279771932096 13 21.873379989631932 16.423665111456714 22.639644893727322 39.06331000518403 14 24.333430524508376 22.80914892927722 28.90789516311919 23.949525383095217 15 30.56795494018537 20.039930527375134 23.271706137289595 26.1204083951499 16 30.99116622092552 24.792932976740417 23.37790744792933 20.837993354404734 17 28.542503563560967 22.14591162368796 27.157574186860177 22.15401062589089 18 25.17858589130963 23.99935206932858 27.859399044302258 22.962662995059528 19 32.181299442355076 24.888146803268057 21.966995201659966 20.963558552716897 20 24.220118036189117 33.418185355729946 21.068162656462803 21.293533951618134 21 23.585914215368668 23.101593883633537 31.438706751328237 21.873785149669562 22 28.07114163535053 22.11999481663859 22.215563042633146 27.593300505377737 23 24.711675521575742 27.360049241933392 22.33542827523649 25.592846961254374 24 25.3514966956071 25.696514189451857 29.70390047946093 19.24808863548011 25 30.111933649749727 26.702088024233394 24.52335055804837 18.66262776796851 26 23.62022322657984 22.836176313359577 29.14742997845491 24.396170481605676 27 26.076031450109426 20.299910837318635 28.67471832698387 24.94933938558807 28 25.992703688690717 26.229428455614105 30.3542764491285 17.42359140656668 29 28.278588906353814 21.509941501515126 31.383383837565425 18.828085754565638 30 20.288047176230435 21.470693062890838 39.26222337426692 18.9790363866118 31 17.606170099000277 29.865353144190415 35.597971385517766 16.93050537129154 32 23.239037043041257 23.127178406419713 36.874442733241466 16.75934181729756 33 16.543914863242595 23.535900846825218 38.72684208818662 21.193342201745562 34 15.833576453045481 28.738370773769006 40.75983014036112 14.668222632824401 35 15.611376711773762 23.438943359533262 47.63633417065068 13.313345758042297 36 16.234565900768057 28.53323395015718 40.77356839614998 14.458631752924783 37 16.629614604462475 22.623935091277893 47.45638945233266 13.290060851926977 38 22.500649603741717 23.078796933870336 41.1621410939327 13.258412368455241 39 15.875488907282081 23.20138922699904 40.98219647175292 19.94092539396595 40 12.668547111669085 18.07672867787325 41.60403045668726 27.6506937537704 41 12.182218956649523 16.61686668299453 39.44485264103192 31.756061719324023 42 20.743239955230415 22.965498223815473 41.93255365050528 14.35870817044883 43 14.140102577419983 19.96364344608193 45.492761150425245 20.403492826072846 44 13.314996256632272 21.59597669346701 40.1923765502425 24.896650499658215 45 14.720251911246734 23.819571815806132 47.236605041471215 14.22357123147592 46 14.817879451610287 23.47854303561288 41.4391173846029 20.264460128173926 47 14.458182761023513 28.311915603882742 42.04411025944352 15.18579137565023 48 15.533382006976556 29.100348827776422 41.5786484951732 13.787620670073823 49 12.270278189246822 24.206859505477514 36.60573307505738 26.917129230218283 50 19.902361489554952 24.656156740625406 41.481769819644484 13.959711950175166 51 14.727813246259563 30.464447585164965 40.81582923144403 13.991909937131439 52 14.759920216647476 25.864725055540237 45.165161269398546 14.21019345841374 53 14.789337919174548 25.749931050146824 45.28626356689758 14.174467463781049 54 14.160297291595668 29.722668484169873 37.38376904727131 18.733265176963148 55 15.16356847146684 32.518873285169256 38.827826934004385 13.489731309359525 56 20.376200986756686 26.997857699298883 39.08238119968839 13.543560114256037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 6.5 11 12.0 12 53.5 13 114.0 14 133.0 15 180.0 16 227.0 17 256.0 18 285.0 19 419.5 20 554.0 21 690.0 22 891.0 23 956.0 24 1049.0 25 1142.0 26 1426.5 27 1711.0 28 2024.0 29 2510.5 30 2684.0 31 2914.5 32 3145.0 33 3120.5 34 3096.0 35 2974.0 36 2852.0 37 2594.0 38 2084.5 39 1833.0 40 1662.0 41 1491.0 42 1435.5 43 1380.0 44 1417.5 45 1455.0 46 1565.5 47 2502.5 48 3329.0 49 3288.5 50 3248.0 51 2746.5 52 2245.0 53 2211.0 54 2272.0 55 2367.0 56 2388.0 57 2409.0 58 2332.5 59 2256.0 60 2326.5 61 2397.0 62 2302.0 63 2113.5 64 2020.0 65 1860.5 66 1701.0 67 1576.5 68 1452.0 69 1261.5 70 1071.0 71 947.5 72 709.5 73 595.0 74 558.0 75 521.0 76 441.5 77 362.0 78 271.0 79 131.0 80 82.0 81 57.5 82 33.0 83 24.5 84 16.0 85 14.0 86 12.0 87 6.5 88 1.5 89 2.0 90 1.0 91 0.0 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01295840352468576 14 0.00323960088117144 15 0.20895425683555785 16 0.06641181806401451 17 0.0 18 0.00161980044058572 19 0.07775042114811456 20 0.09718802643514318 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00485940132175716 26 0.0080990022029286 27 0.08260982246987171 28 0.09880782687572891 29 0.04049501101464299 30 0.0161980044058572 31 0.030776208371128676 32 0.08260982246987171 33 0.15226124141505767 34 0.06155241674225735 35 0.050213813658157316 36 0.03563560969288584 37 0.1797978489050149 38 0.2591680704937152 39 0.19275625242970068 40 0.6527795775560451 41 0.7953220163275885 42 0.14092263833095764 43 0.20085525463262927 44 0.47622132953220164 45 0.2057146559543864 46 0.1635998444991577 47 0.043734611895814436 48 0.1635998444991577 49 0.49079953349747313 50 0.1295840352468576 51 0.28994427886484386 52 0.11176623040041467 53 0.1571206427368148 54 0.18303744978618633 55 0.22839186212258647 56 0.19437605287028636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 61736.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.78573279771932 #Duplication Level Percentage of deduplicated Percentage of total 1 86.83431623456104 63.2029933912142 2 7.5931901635696 11.053518206556951 3 2.5303215756092134 5.525139302837891 4 1.0771113831089352 3.1359336529739537 5 0.5852898631356404 2.1300375793702213 6 0.3226883275842884 1.4092263833095764 7 0.2269945476799822 1.156537514578204 8 0.14687882496940025 0.85525463262926 9 0.12684989429175475 0.8309576260204742 >10 0.5207521976187827 5.83938058831152 >50 0.02670524090352732 1.28288194894389 >100 0.00667631022588183 1.5825450304522481 >500 0.0 0.0 >1k 0.0022254367419606096 1.9955941428016069 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1232 1.9955941428016069 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 370 0.5993261630167164 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 328 0.5312945445121161 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 279 0.4519243229234159 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 95 0.15388104185564339 No Hit GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 79 0.12796423480627186 No Hit GAGGAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 77 0.12472463392510043 No Hit GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.1231048334845147 No Hit GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 0.1133860308410004 No Hit GAGGAGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA 70 0.1133860308410004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.012958403524685759 0.0 0.0 0.0 0.0 6 0.012958403524685759 0.0 0.0 0.0 0.0 7 0.014578203965271479 0.0 0.0 0.0 0.0 8 0.014578203965271479 0.0 0.0 0.0 0.0 9 0.01781780484644292 0.0 0.0 0.0 0.0 10 0.027536607489957236 0.0 0.0 0.0 0.0 11 0.04049501101464299 0.0 0.0 0.0 0.0 12 0.04697421277698587 0.0 0.0 0.0 0.0 13 0.04697421277698587 0.0 0.0 0.0 0.0 14 0.04697421277698587 0.0 0.0 0.0 0.0 15 0.0485940132175716 0.0 0.0 0.0 0.0 16 0.051833614098743035 0.0 0.0 0.0 0.0 17 0.05345341453932875 0.0 0.0 0.0 0.0 18 0.05993261630167163 0.0 0.0 0.0 0.0 19 0.05993261630167163 0.0 0.0 0.0 0.0 20 0.06803161850460024 0.0 0.0 0.0 0.0 21 0.07289101982635739 0.0 0.0 0.0 0.0 22 0.07775042114811455 0.0 0.0 0.0 0.0 23 0.09070882467280031 0.0 0.0 0.0 0.0 24 0.09232862511338603 0.0 0.0 0.0 0.0 25 0.09232862511338603 0.0 0.0 0.0 0.0 26 0.09556822599455747 0.0 0.0 0.0 0.0 27 0.1052870286380718 0.0 0.0 0.0 0.0 28 0.11176623040041467 0.0 0.0 0.0 0.0 29 0.11986523260334327 0.0 0.0 0.0 0.0 30 0.1231048334845147 0.0 0.0 0.0 0.0 31 0.12634443436568615 0.0 0.0 0.0 0.0 32 0.12634443436568615 0.0 0.0 0.0 0.0 33 0.12634443436568615 0.0 0.0 0.0 0.0 34 0.12796423480627186 0.0 0.0 0.0 0.0 35 0.12958403524685758 0.0 0.0 0.0 0.0 36 0.13444343656861474 0.0 0.0 0.0 0.0 37 0.1376830374497862 0.0 0.0 0.0 0.0 38 0.1393028378903719 0.0 0.0 0.0 0.0 39 0.14092263833095764 0.0 0.0 0.0 0.0 40 0.14092263833095764 0.0 0.0 0.0 0.0 41 0.14254243877154335 0.0 0.0 0.0 0.0 42 0.14902164053388622 0.0 0.0 0.0 0.0 43 0.16036024361798626 0.0 0.0 0.0 0.0 44 0.16845924582091487 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGTC 20 3.9804744E-4 50.43057 38 AAACACC 15 0.00811325 50.43057 38 GTCTGGC 15 0.008139665 50.389164 37 ATGCCTG 25 1.990798E-5 50.306557 36 ATGCCCG 15 0.008192686 50.306557 36 TATGCTA 20 4.0455523E-4 50.265358 35 GTGAAGG 15 0.008219292 50.265358 34 CTACCCG 15 0.008219292 50.265358 34 TGCCAAG 15 0.008245964 50.224224 47 CAACGAT 55 0.0 50.224224 46 AACGATT 50 7.2759576E-12 50.22422 47 ACGATTA 50 7.2759576E-12 50.22422 48 CGATTAA 50 7.2759576E-12 50.22422 49 TAGCGCC 15 0.008272699 50.183155 44 CCAAAAC 35 5.032598E-8 50.183155 43 CACAATT 15 0.008353292 50.06036 42 GCAGCAT 30 1.0230287E-6 50.06036 42 TGTAGCG 15 0.008353292 50.06036 42 TTCGGCG 15 0.008353292 50.06036 33 TGCTACC 15 0.008407344 49.97883 32 >>END_MODULE