FastQCFastQC Report
Fri 17 Jun 2016
SRR1526203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12228
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGAATCTAAAGAGACAAAGTTAGGAATGGGAATCTTCTACAAACAAATT620.5070330389270526No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG480.39254170755642787No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC330.2698724239450442No Hit
GCTCAAGGGGGACTCCAGGCTGCATCATATATCATAATTTATTTTAAAGAGAAAGT290.23716061498200852No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG260.21262675825973174No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG250.20444880601897283No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG250.20444880601897283No Hit
GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG200.16355904481517827No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG180.14720314033366044No Hit
GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC170.13902518809290154No Hit
GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG170.13902518809290154No Hit
GCTCAAGGGGGACTCCAGGCTGCATCATATATCATAATTTCTTTTAAAGAGAAAGT160.13084723585214264No Hit
GCTCAAGGGAAGTAACGCAGAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13084723585214264No Hit
GCTCAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA160.13084723585214264No Hit
GCTCAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13084723585214264No Hit
GCTCAAGGGTGGTTCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12266928361138371No Hit
GCTCAAGGGAGAGTGGTCACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12266928361138371No Hit
GCTCAAGGGAAGCATGGATCACGCAGAGTCGCTTTTTTTTTTTTTTTTTTTTTTTT150.12266928361138371No Hit
GCTCAAGGGAAGATGGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT150.12266928361138371No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12266928361138371No Hit
GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG140.11449133137062481No Hit
GCTCAAGGGAGTGTTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11449133137062481No Hit
GCTCAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG140.11449133137062481No Hit
GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC140.11449133137062481No Hit
GCTCAAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11449133137062481No Hit
GCTCAAGGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10631337912986587No Hit
GCTCAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG130.10631337912986587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATG150.008160992550.111118
CTACAAA203.977526E-450.1111144
ATGGGAA150.008160992550.1111133
ACAAACA203.977526E-450.1111146
AACAAAT203.977526E-450.1111149
AATCTAA203.977526E-450.1111110
CACACGC150.008160992550.1111141
GATACTC150.008160992550.111119
ATCTAAA203.977526E-450.1111111
GAAGCAT203.977526E-450.111119
TCGGACA150.008160992550.1111136
TCTACAA203.977526E-450.1111143
GGGAGAT251.941288E-550.111117
GGATACT150.008160992550.111118
AACCAGC150.008160992550.1111133
AGCGGTG150.008160992550.1111147
TCTAAAG203.977526E-450.1111112
TACAAAC251.941288E-550.1111145
GGCTTTT251.941288E-550.111119
GAATCTA203.977526E-450.111119
Produced by FastQC (version 0.11.5)