##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526203_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12228 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.280421982335625 39.0 37.0 40.0 33.0 40.0 2 36.67198233562316 39.0 37.0 40.0 30.0 40.0 3 36.86261040235525 39.0 37.0 40.0 31.0 40.0 4 36.81207065750736 39.0 37.0 40.0 31.0 40.0 5 34.65480863591757 39.0 35.0 39.0 18.0 40.0 6 33.773961400065424 38.0 32.0 39.0 17.0 40.0 7 36.3976120379457 38.0 35.0 39.0 30.0 40.0 8 36.5247791952895 38.0 36.0 39.0 30.0 40.0 9 36.571148184494604 39.0 36.0 39.0 30.0 40.0 10 36.21630683676808 38.0 36.0 39.0 29.0 40.0 11 35.659633627739616 38.0 35.0 39.0 29.0 40.0 12 33.68727510631338 38.0 31.0 39.0 20.0 40.0 13 33.51995420346745 38.0 31.0 39.0 20.0 40.0 14 33.65439973830553 38.0 31.0 39.0 20.0 40.0 15 33.661432777232584 38.0 31.0 39.0 20.0 40.0 16 33.96671573438011 38.0 33.0 39.0 22.0 40.0 17 33.82883545960092 38.0 32.0 39.0 22.0 40.0 18 33.38828917239123 38.0 31.0 39.0 20.0 40.0 19 34.154236179260714 38.0 32.0 39.0 24.0 40.0 20 34.065505397448476 38.0 32.0 39.0 23.0 40.0 21 34.86776251226693 38.0 34.0 39.0 25.0 40.0 22 34.693245011449136 38.0 33.0 39.0 25.0 40.0 23 35.14924762839385 38.0 34.0 39.0 27.0 40.0 24 34.81951259404645 38.0 33.0 39.0 25.0 40.0 25 34.973421655217535 38.0 34.0 39.0 26.0 40.0 26 35.65807981681387 38.0 35.0 39.0 29.0 40.0 27 35.48724239450441 38.0 35.0 39.0 28.0 40.0 28 35.493702976774614 38.0 35.0 39.0 28.0 40.0 29 35.136081125286225 38.0 35.0 39.0 27.0 40.0 30 35.33447824664704 38.0 35.0 39.0 27.0 40.0 31 35.65489041543997 38.0 35.0 39.0 29.0 40.0 32 35.304138043833824 38.0 35.0 39.0 28.0 40.0 33 35.42508995747465 38.0 35.0 39.0 29.0 40.0 34 35.37095191364082 38.0 35.0 39.0 29.0 40.0 35 35.19537127903173 38.0 35.0 39.0 28.0 40.0 36 34.599852796859665 38.0 34.0 39.0 28.0 40.0 37 34.47505724566569 38.0 34.0 39.0 28.0 40.0 38 34.09813542688911 38.0 33.0 39.0 26.0 40.0 39 33.60426889106968 37.0 33.0 39.0 24.0 40.0 40 30.403990840693492 35.0 26.0 39.0 10.0 40.0 41 26.76733725875041 32.0 15.0 38.0 6.0 39.0 42 31.191445861956165 35.0 30.0 38.0 13.0 39.0 43 29.749754661432778 34.0 27.0 38.0 6.0 39.0 44 29.57875368007851 35.0 26.0 38.0 5.0 39.0 45 30.786228328426564 35.0 29.0 38.0 4.0 39.0 46 30.394259077526986 35.0 28.0 38.0 4.0 40.0 47 29.895403990840695 35.0 27.0 38.0 4.0 40.0 48 29.938174681059863 35.0 27.0 38.0 4.0 40.0 49 27.242803402028134 33.0 16.0 38.0 4.0 40.0 50 29.73773307163886 35.0 27.0 38.0 4.0 40.0 51 29.8976120379457 35.0 28.0 39.0 4.0 40.0 52 29.544488060189728 35.0 27.0 39.0 4.0 40.0 53 29.20322211318286 35.0 26.0 39.0 4.0 40.0 54 28.211890742558065 35.0 19.0 39.0 4.0 40.0 55 28.453958128884526 35.0 23.0 39.0 4.0 40.0 56 27.15055610075237 33.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3666470964893804 1 2 2.195863355949463 1 3 2.0859264897684113 1 4 1.9481491252340675 1 5 4.562100752274894 1 6 6.030193874609505 1 7 2.46435764731914 1 8 1.6371938360773512 1 9 2.33491000567917 1 10 2.69115408488571 1 11 -1.20837582606449 1 12 -8.782687146409106 1 13 -9.510091357301103 1 14 -2.665932509935036 1 15 -4.714232930346498 1 16 -25.69261791377697 1 17 -6.297127999995656 1 18 -18.232796385402796 1 19 -5.1976454616934475 1 20 -7.731872373663194 1 21 -8.98177174913437 1 22 -6.5233870503278375 1 23 -4.754542760086494 1 24 -5.26079165194685 1 25 -5.016066761684026 1 26 1.6833495634124347 1 27 0.7901070754079669 1 28 -1.460255869109389 1 29 1.3878200739462443 1 30 1.1131593533673652 1 31 1.4630552835680533 1 32 2.3139565310134316 1 33 1.7941728962113999 1 34 1.0283418162433264 1 35 0.4676305235370606 1 36 2.2998475634988935 1 37 2.729376147266045 1 38 3.4857262076660334 1 39 1.999410521037852 1 40 5.308691333355618 1 41 -6.365016054600993 1 42 -7.032997836249585 1 43 -15.128492528713103 1 44 -23.784715608071835 1 45 -6.667771384711205 1 46 -11.607980506657935 1 47 -22.692458345246663 1 48 -7.679826606213986 1 49 -19.434570108540687 1 50 -8.605200099393205 1 51 -5.390754380566683 1 52 -5.168262860469703 1 53 -5.3023614158531025 1 54 -5.65163484026294 1 55 -4.817442104965316 1 56 -0.07347433282805582 2 1 2.3110915409338233 2 2 3.183042843128952 2 3 2.86370426754619 2 4 3.311396988481931 2 5 4.946716136890281 2 6 6.363527207942841 2 7 3.263502946464442 2 8 2.961980160863675 2 9 3.356277527046693 2 10 3.19115408488571 2 11 3.197607079918413 2 12 -0.6758495395715016 2 13 -0.6553905026002482 2 14 4.577657233654705 2 15 4.537903821790252 2 16 3.4013991802401264 2 17 4.984923282055625 2 18 0.4552378026313946 2 19 4.12286735881937 2 20 3.8407771989863804 2 21 3.9541256867630636 2 22 3.7330232060824216 2 23 3.288192282648545 2 24 1.234934843779648 2 25 3.518121272504011 2 26 3.435486315549184 2 27 3.6832694685703586 2 28 3.3217954129418885 2 29 3.195512381638551 2 30 3.899484139692156 2 31 2.903226223738997 2 32 2.2712214882783925 2 33 2.247164349202855 2 34 3.4257777136792242 2 35 3.5360065919131287 2 36 2.7613860250373534 2 37 3.780658198548096 2 38 2.853247575187396 2 39 1.7130857347130615 2 40 7.035187059851346 2 41 -5.818007507592444 2 42 -6.515903819155568 2 43 -11.927637827858401 2 44 -13.280442103798332 2 45 -6.0609337778736005 2 46 -9.150715549392977 2 47 -16.850578003366323 2 48 -7.782390708778088 2 49 -11.045681219651797 2 50 -6.579559073752179 2 51 -4.852292842105143 2 52 -4.386211578418418 2 53 -4.105780219271907 2 54 -2.9678741565022584 2 55 -5.467014754537967 2 56 -0.38544014479386846 3 1 2.699980429822716 3 2 3.683042843128952 3 3 3.3081487119906328 3 4 3.311396988481931 3 5 5.8356050257791665 3 6 6.863527207942841 3 7 3.430169613131106 3 8 3.2397579386414534 3 9 3.6896108603800286 3 10 3.746709640441267 3 11 4.030940413251749 3 12 6.157483793761834 3 13 5.066831719621973 3 14 5.077657233654705 3 15 5.982348266234695 3 16 4.290288069129012 3 17 4.373812170944518 3 18 5.621904469298059 3 19 5.011756247708263 3 20 4.785221643430823 3 21 4.620792353429735 3 22 4.06635653941575 3 23 3.9548589493152164 3 24 4.901601510446312 3 25 3.1847879391706755 3 26 3.1021529822158556 3 27 3.849936135237023 3 28 3.8217954129418885 3 29 3.751067937194108 3 30 3.455039695247713 3 31 2.9587817792945543 3 32 3.437888154945057 3 33 2.747164349202855 3 34 2.314666602568117 3 35 2.4248954808020216 3 36 3.3169415805929106 3 37 3.1139915318814317 3 38 3.1310253529651746 3 39 4.49086351249084 3 40 8.70185372651801 3 41 10.237548047963113 3 42 6.7618739586222105 3 43 0.2390288388082631 3 44 3.0528912295349997 3 45 2.772399555459735 3 46 2.6270622283847977 3 47 -4.683911336699655 3 48 2.2731648467774654 3 49 5.787652113681535 3 50 2.420440926247821 3 51 2.481040491228189 3 52 2.280455088248246 3 53 1.949775336283647 3 54 2.1432369546088523 3 55 1.7552074676842544 3 56 3.336782077428353 4 1 2.682436570173593 4 2 3.6128674045324587 4 3 3.0859264897684113 4 4 3.5716309066105865 4 5 6.051979294785021 4 6 7.188088611451608 4 7 3.1202280926632753 4 8 3.353793026360748 4 9 3.0434120299706677 4 10 3.69115408488571 4 11 4.30579421442134 4 12 6.496665080311537 4 13 6.408936982779871 4 14 5.358358988040671 4 15 5.909248851030021 4 16 5.056369940474042 4 17 5.318256615388961 4 18 6.002021428362383 4 19 4.798305955310603 4 20 5.413876614191054 4 21 3.892722177991139 4 22 4.715479346433298 4 23 3.7150928674438717 4 24 4.492244785300116 4 25 4.222799635077109 4 26 3.3068313447889537 4 27 3.931807480266265 4 28 2.681444535748909 4 29 3.642880802691181 4 30 2.080770689399756 4 31 3.268723299762385 4 32 2.5811630087462234 4 33 3.4459947585595785 4 34 0.8906899943809776 4 35 2.050626474954065 4 36 4.2350702355636685 4 37 4.271886268723534 4 38 4.303539972848213 4 39 4.663378132373879 4 40 8.976707527687601 4 41 12.088425240945565 4 42 7.00748799370993 4 43 7.79458439436382 4 44 8.14645848099699 4 45 5.822107157798918 4 46 5.448699655285385 4 47 4.275152990785724 4 48 6.445679466660504 4 49 7.565429891459313 4 50 6.508160224493434 4 51 7.305601894736959 4 52 6.657648070704386 4 53 7.7948045760497315 4 54 6.283587831801835 4 55 6.696727935520514 4 56 11.371869796726603 5 1 2.6019412141364384 5 2 2.467356568619145 5 3 3.2623970780037084 5 4 2.72642966822049 5 5 5.476127901596165 5 6 6.736076227550679 5 7 2.8353983713010464 5 8 2.465248134719886 5 9 2.705950729661076 5 10 3.985271731944536 5 11 4.017868517826912 5 12 5.719575297029806 5 13 6.390361131386676 5 14 5.5286376258115695 5 15 5.828753494992867 5 16 4.257608330566924 5 17 5.788844850683077 5 18 5.961773750343809 5 19 3.7797301039174087 5 20 4.906136676110563 5 21 2.9051060789199283 5 22 4.282042813925557 5 23 3.7522445702302463 5 24 3.8297060856097076 5 25 3.55406898492231 5 26 3.0498654005165093 5 27 2.640785808439638 5 28 1.9198346286281662 5 29 2.695512381638551 5 30 3.4027521135483667 5 31 3.259435374065795 5 32 3.3365809654025753 5 33 2.9072950681571044 5 34 2.200287517600792 5 35 3.9020196638085523 5 36 3.1731507309197085 5 37 3.8786974142343738 5 38 3.997038424860598 5 39 3.8862883490921476 5 40 8.571134772269644 5 41 9.698332361688603 5 42 6.957952389994759 5 43 7.559290276716762 5 44 7.360080772018662 5 45 5.952138117551236 5 46 5.885232163025325 5 47 6.5971344149343345 5 48 6.315648506908186 5 49 9.74190047969461 5 50 8.263578181149786 5 51 8.265354216718386 5 52 7.564768813738443 5 53 6.181801480074501 5 54 5.80990362127552 5 55 5.702919885984912 5 56 8.356389920565608 6 1 1.9666470964893819 6 2 2.483042843128949 6 3 3.1525931564350813 6 4 3.122508099593041 6 5 5.480049470223612 6 6 6.76352720794284 6 7 2.6079473909088904 6 8 2.6064246053081206 6 9 1.8784997492689186 6 10 3.2911540848857115 6 11 1.9198293021406343 6 12 4.135261571539608 6 13 6.43349838628864 6 14 5.744323900321376 6 15 5.726792710679142 6 16 2.7125102913512364 6 17 4.518256615388964 6 18 4.84412669152028 6 19 1.9562006921527058 6 20 3.4629994212086004 6 21 1.8541256867630693 6 22 3.199689872749083 6 23 2.732636727092995 6 24 3.3904903993351994 6 25 2.1070101613928998 6 26 2.7243752044380756 6 27 1.9388250241259115 6 28 3.0884620796085542 6 29 3.8288457149718838 6 30 2.87726191746993 6 31 1.5143373348501044 6 32 2.0934437105006083 6 33 2.0916087936473033 6 34 1.7924443803458914 6 35 0.38045103635757727 6 36 1.9613860250373563 6 37 2.913991531881429 6 38 4.27546979740962 6 39 1.9019746236019515 6 40 6.724075948740229 6 41 4.737548047963109 6 42 4.361873958622205 6 43 7.194584394363819 6 44 7.697335673979445 6 45 4.450177333237512 6 46 5.171506672829242 6 47 5.204977552189231 6 48 6.81760929122191 6 49 10.165429891459315 6 50 6.553774259581157 6 51 6.947707157894854 6 52 5.913788421581582 6 53 7.27199755850587 6 54 4.87657028794219 6 55 3.6440963565731437 6 56 2.8034487440950215 7 1 2.3078235670776124 7 2 3.349709509795616 7 3 3.2035735485919403 7 4 3.079370844691077 7 5 5.887892607478513 7 6 6.853723286374208 7 7 3.3059866065951624 7 8 3.1711304876610598 7 9 2.529480141425779 7 10 3.161742320179826 7 11 3.606103811944557 7 12 4.366634120559219 7 13 5.449184660798444 7 14 5.0580493905174535 7 15 5.946400553816396 7 16 4.257608330566924 7 17 4.43590367421249 7 18 6.020597279755577 7 19 4.544435986270351 7 20 2.141430793757621 7 21 3.4933413730375733 7 22 2.340866343337318 7 23 2.2816563349361303 7 24 2.0061766738450046 7 25 2.671716043745839 7 26 -0.00895812889525871 7 27 1.052550514321993 7 28 2.0374816874516952 7 29 2.166100616932667 7 30 0.34392858413659866 7 31 2.024141256418737 7 32 1.2189339065790463 7 33 1.6720009505100464 7 34 0.25911104701255994 7 35 -0.9803332773679188 7 36 2.1731507309197085 7 37 0.1728150612931998 7 38 1.5852737189782502 7 39 2.4745236432097926 7 40 3.5123112428578764 7 41 -3.7722558736055163 7 42 3.957952389994759 7 43 4.323996159069704 7 44 5.065963124959836 7 45 1.0109616469630005 7 46 3.355820398319441 7 47 5.6559579443460954 7 48 3.84506027161407 7 49 8.03601812675343 7 50 4.557695828208605 7 51 6.735942452012502 7 52 5.211827637267856 7 53 5.711213244780378 7 54 7.104021268334346 7 55 4.526449297749615 7 56 3.650507567624434 8 1 2.8041470964893804 8 2 3.537209509795616 8 3 3.3359264897684113 8 4 3.630841432926374 8 5 5.884216136890281 8 6 7.217693874609505 8 7 3.4162807242422204 8 8 3.6564246053081177 8 9 3.78683308260225 8 10 3.87865408488571 8 11 4.44066263547397 8 12 6.789428238206277 8 13 6.504331719621973 8 14 6.6609905669880405 8 15 6.718459377345809 8 16 5.345843624684569 8 17 5.818256615388961 8 18 5.65662669152028 8 19 5.518700692152706 8 20 4.917166087875266 8 21 4.474959020096399 8 22 4.212189872749086 8 23 4.274303393759659 8 24 4.248823732668534 8 25 3.844510161392897 8 26 3.5535418711047413 8 27 3.90549169079258 8 28 3.342628746275224 8 29 4.445512381638551 8 30 3.7814285841365987 8 31 3.22267066818344 8 32 3.4726103771672783 8 33 2.2124421269806334 8 34 2.57161104701256 8 35 2.1262843696909073 8 36 2.6363860250373534 8 37 4.051491531881432 8 38 3.158803130742953 8 39 4.018641290268619 8 40 7.9865759487402315 8 41 -3.533285285370223 8 42 4.491040625288875 8 43 6.60708439436382 8 44 6.518169007312778 8 45 5.654343999904178 8 46 6.738173339495912 8 47 7.6633108855225665 8 48 6.613442624555244 8 49 9.127929891459313 8 50 6.399607592914489 8 51 6.210207157894857 8 52 6.822121754914914 8 53 7.192830891839204 8 54 9.43490362127552 8 55 8.956596356573144 8 56 11.81594874409502 9 1 3.0095042393465263 9 2 3.992566652652762 9 3 3.514497918339842 9 4 3.89869857578352 9 5 6.589573279747427 9 6 7.601622446038075 9 7 4.11270929567079 9 8 3.977853176736687 9 9 3.9832616540308194 9 10 4.048296942028564 9 11 4.75316263547397 9 12 7.101928238206277 9 13 6.7811174339076885 9 14 6.839561995559471 9 15 6.307745091631524 9 16 5.345843624684569 9 17 6.38968518681753 9 18 6.772698120091711 9 19 5.384772120724136 9 20 5.372523230732412 9 21 4.287459020096399 9 22 5.042547015606232 9 23 4.542160536616805 9 24 4.7666808755256795 9 25 3.8355815899643275 9 26 3.4910418711047413 9 27 3.1912059765068648 9 28 3.583700174846655 9 29 3.1240838102099815 9 30 3.6296428698508834 9 31 3.490527811040586 9 32 4.231538948595848 9 33 2.6677992698377793 9 34 3.6876824755839905 9 35 4.242355798262338 9 36 3.689957453608784 9 37 4.613991531881432 9 38 4.265945987885807 9 39 5.054355575982903 9 40 8.352647377311662 9 41 3.8327861432012043 9 42 5.499969196717444 9 43 7.651727251506674 9 44 7.830669007312778 9 45 7.145415428475609 9 46 7.809601910924481 9 47 8.89545374266542 9 48 8.122371195983813 9 49 11.636858462887883 9 50 9.51567902148592 9 51 6.219135729323426 9 52 6.304264612057768 9 53 7.005330891839204 9 54 8.167046478418374 9 55 7.858382070858859 9 56 6.932020172666451 10 1 1.5431176847246775 10 2 2.290885980383848 10 3 2.4388676662389983 10 4 2.902900256455787 10 5 5.123186725125578 10 6 6.61842916872715 10 7 2.2471630771833944 10 8 2.1123069582492917 10 9 2.941244847308134 10 10 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-16.280723954694533 115 50 -6.451353945547051 115 51 -6.544600534412837 115 52 -8.168262860469703 115 53 -5.225438338930026 115 54 -10.343942532570633 115 55 -4.27898056650378 115 56 -2.5350127943665193 116 1 1.6999804298227161 116 2 2.0163761764622805 116 3 1.352593156435077 116 4 2.0558414329263712 116 5 5.2133828035569465 116 6 5.830193874609503 116 7 2.074614057575552 116 8 2.006424605308119 116 9 1.6118330826022529 116 10 1.62448741821904 116 11 -2.0135040311926957 116 12 -11.864738428460388 116 13 -10.766501613711359 116 14 -4.322342766345294 116 15 -11.073207289320859 116 16 -4.220823041982097 116 17 -4.081743384611038 116 18 -11.622539975146385 116 19 -4.043799307847294 116 20 -5.737000578791399 116 21 1.3874590200964008 116 22 1.5996898727490887 116 23 1.5326367270929921 116 24 0.6571570660018651 116 25 2.04034349472623 116 26 0.8577085377714084 116 27 0.8721583574592486 116 28 1.48846207960856 116 29 2.762179048305221 116 30 1.6105952508032644 116 31 0.7810040015167701 116 32 1.6267770438339468 116 33 0.6249421269806348 116 34 0.8591110470125614 116 35 -0.15288229697576128 116 36 2.028052691704019 116 37 1.913991531881429 116 38 2.675469797409619 116 39 2.8353079569352886 116 40 5.457409282073563 116 41 3.7375480479631094 116 42 -2.1714593747111266 116 43 -6.4720822723028455 116 44 -5.4359976593538875 116 45 -2.149822666762489 116 46 -2.4951599938374223 116 47 -2.1283557811441014 116 48 -3.315724042111423 116 49 -4.634570108540686 116 50 -4.712892407085512 116 51 -4.71895950877181 116 52 -5.619544911751753 116 53 -7.594669108160797 116 54 -10.523429712057812 116 55 -5.622570310093522 116 56 -2.129884589238312 117 1 -0.5222417923995053 117 2 1.5719317320178376 117 3 1.3081487119906328 117 4 1.4780636551485955 117 5 4.168938359112502 117 6 4.363527207942841 117 7 1.319058502019999 117 8 -1.1491309502474394 117 9 -0.6992780285088642 117 10 1.69115408488571 117 11 -1.0801706978593657 117 12 -11.95362731734928 117 13 -12.655390502600248 117 14 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0.059111047012557094 118 35 1.8137843696909073 118 36 2.5613860250373506 118 37 1.5139915318814303 118 38 0.9088031307429532 118 39 3.36864129026862 118 40 5.124075948740234 118 41 3.404214714629777 118 42 -1.371459374711126 118 43 -7.70541560563618 118 44 -7.669330992687222 118 45 -7.583156000095823 118 46 -12.261826660504088 118 47 -13.661689114477433 118 48 -15.649057375444755 118 49 -11.834570108540687 118 50 -10.81289240708551 118 51 -10.152292842105144 118 52 -13.852878245085087 118 53 -10.594669108160797 118 54 -15.19009637872448 118 55 -10.755903643426855 118 56 -7.896551255904978 119 1 0.25553598537826616 119 2 2.127487287573395 119 3 2.0859264897684113 119 4 0.5891747662597098 119 5 4.8356050257791665 119 6 5.69686054127617 119 7 1.319058502019999 119 8 0.2953134941970035 119 9 2.0784997492689143 119 10 2.135598529330153 119 11 -2.4135040311926943 119 12 -11.620293984015944 119 13 -7.766501613711359 119 14 -8.255676099678627 119 15 -9.739873955987523 119 16 -10.709711930870988 119 17 -12.348410051277707 119 18 -16.26698441959083 119 19 -7.266021530069516 119 20 -6.714778356569177 119 21 0.7874590200963993 119 22 0.955245428304643 119 23 1.288192282648545 119 24 2.0127126215574194 119 25 0.018121272504011188 119 26 1.2132640933269627 119 27 1.9610472463481372 119 28 1.04401763516411 119 29 2.1399568260829938 119 30 1.8994841396921558 119 31 0.7365595570723258 119 32 -2.6176674006105003 119 33 0.9138310158695191 119 34 -0.07422228632077577 119 35 0.20267325857979301 119 36 1.0947193583706891 119 37 0.002880420770317471 119 38 -1.2578635359237111 119 39 -0.009136487509159963 119 40 5.812964837629117 119 41 -2.2624519520368906 119 42 -5.793681596933347 119 43 -14.983193383413958 119 44 -18.16933099268722 119 45 -15.172044888984711 119 46 -17.761826660504088 119 47 -23.1283557811441 119 48 -16.782390708778088 119 49 -17.99012566409624 119 50 -17.135114629307733 119 51 -15.185626175438477 119 52 -15.108433800640642 119 53 -15.105780219271907 119 54 -18.96787415650226 119 55 -16.911459198982413 119 56 -15.99655125590498 120 1 -0.13335290351061957 120 2 0.5997095097956162 120 3 1.2109264897684113 120 4 0.6308414329263741 120 5 3.446716136890281 120 6 4.780193874609505 120 7 0.6662807242422204 120 8 0.5314246053081177 120 9 0.78683308260225 120 10 1.81615408488571 120 11 -4.12183736452603 120 12 -8.023071761793723 120 13 -5.933168280378027 120 14 -16.71400943301196 120 15 -10.406540622654191 120 16 -22.02915637531543 120 17 -15.306743384611039 120 18 -17.78087330847972 120 19 -7.043799307847294 120 20 -12.770333912124734 120 21 -1.5875409799036007 120 22 -3.225310127250914 120 23 -1.7256966062403407 120 24 -5.376176267331466 120 25 -0.8429898386071031 120 26 1.7410418711047413 120 27 1.03049169079258 120 28 1.5301287462752242 120 29 1.445512381638551 120 30 1.4689285841365987 120 31 -1.52732933181656 120 32 -16.46488962283272 120 33 -3.3500578730193666 120 34 -2.74088895298744 120 35 -4.811215630309093 120 36 -0.8636139749626466 120 37 -2.0110084681185683 120 38 -2.341196869257047 120 39 -0.1063587097313814 120 40 3.2990759487402315 120 41 3.279214714629777 120 42 -5.071459374711125 120 43 -3.8304156056361798 120 44 -11.794330992687222 120 45 -4.658156000095822 120 46 -10.261826660504088 120 47 -12.711689114477434 120 48 -7.824057375444756 120 49 -16.684570108540687 120 50 -7.662892407085511 120 51 -4.602292842105143 120 52 -3.302878245085086 120 53 -5.494669108160796 120 54 -3.31509637872448 120 55 -4.605903643426856 120 56 -0.37155125590497917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 100.0 5 8.0 6 4.0 7 7.0 8 7.0 9 10.0 10 10.0 11 15.0 12 10.0 13 11.0 14 23.0 15 35.0 16 48.0 17 53.0 18 56.0 19 81.0 20 89.0 21 145.0 22 146.0 23 179.0 24 201.0 25 267.0 26 254.0 27 279.0 28 341.0 29 361.0 30 437.0 31 487.0 32 600.0 33 721.0 34 818.0 35 980.0 36 1281.0 37 1551.0 38 1630.0 39 982.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 97.22767419038273 2.739614000654236 0.0 0.03271180896303566 8 99.56656853123977 0.40071965979718677 0.01635590448151783 0.01635590448151783 9 99.1822047759241 0.5561007523716062 0.13902518809290154 0.12266928361138371 10 46.11547268563952 39.16421328099444 7.360157016683022 7.360157016683022 11 32.30291135099771 38.15832515538109 14.524043179587832 15.014720314033367 12 37.25875040889761 15.57082106640497 25.482499182204776 21.68792934249264 13 22.54581151832461 15.453206806282724 28.992146596858635 33.008835078534034 14 24.141315014720313 22.006869479882237 28.115799803729146 25.7360157016683 15 23.811085601836666 22.892751721876024 26.36930141029846 26.92686126598885 16 25.783487439653058 20.95573193683005 23.99149005809672 29.26929056542018 17 31.602191870450643 22.73656661486873 22.13134865461683 23.529892860063793 18 29.661432777232584 22.73470722930978 21.39352306182532 26.210336931632323 19 30.223422538669286 26.417873803093546 20.664538832965054 22.694164825272118 20 25.407219448309732 24.695096995989196 24.424981583040026 25.472701972661042 21 25.016355904481518 27.77232580961727 23.070003271180898 24.141315014720313 22 26.545632973503437 21.02551521099117 23.57703631010795 28.85181550539745 23 30.904481517827936 22.064115145567552 23.56068040562643 23.470722930978084 24 23.96957801766438 21.91691200523389 23.536146548904156 30.57736342819758 25 24.345763820739286 28.60647693817468 25.989532221131828 21.058227019954202 26 23.55442872331725 19.95583544614378 28.412529647501433 28.07720618303754 27 21.08364707808152 23.498117531510886 32.41938124079227 22.998854149615322 28 25.223086369218173 20.253786328284896 34.38395415472779 20.13917314776914 29 20.86878272251309 19.731675392670155 39.52879581151832 19.870746073298427 30 19.707133507853403 16.37761780104712 44.23265706806283 19.68259162303665 31 20.0 17.971370143149283 39.672801635991824 22.355828220858896 32 21.315099901735998 16.893219783819195 45.054045201441205 16.737635113003602 33 24.08484153631971 15.084759642944887 43.46081402014577 17.36958480058963 34 17.27495908346972 21.497545008183305 46.60392798690671 14.623567921440262 35 20.211112020292937 17.78905163243597 49.4394894034858 12.560346943785289 36 18.098510882016036 17.517591228931433 50.687285223367695 13.696612665684832 37 19.082343301925437 16.8455551003687 45.653420729209344 18.418680868496516 38 21.767647782241532 18.881692219398214 47.71665163564811 11.634008362712143 39 22.950147589373564 16.456215152509017 46.449655624795014 14.1439816333224 40 13.454245671887882 15.9439406430338 41.97032151690025 28.63149216817807 41 14.314033203931608 13.438506649046007 38.803997687288344 33.443462459734036 42 15.535173204487757 20.11301285725985 47.80935222340513 16.54246171484727 43 13.402737929338471 14.370030330354947 47.76621034511025 24.46102139519633 44 14.277494657241494 17.84481341443367 45.7586717080388 22.119020220286043 45 14.996312382201099 16.807342456772925 49.373104974186674 18.823240186839303 46 19.70988362563514 18.775610555646615 46.69726274381249 14.817243074905754 47 15.25811993782214 18.129755379203143 46.09343041806431 20.518694264910415 48 16.889180113031372 22.221312146776967 47.14554836595954 13.743959374232123 49 17.813399095766545 15.388409371146732 41.08508014796548 25.713111385121252 50 18.01801801801802 20.26208026208026 46.38001638001638 15.33988533988534 51 20.211544768776648 18.850442768120697 45.68711052804198 15.250901935060677 52 14.886996396986572 23.771699967245333 46.42974123812643 14.911562397641664 53 14.186198983773151 21.660383543681363 50.4343550237666 13.719062448778887 54 17.200951053537754 20.267278839058786 42.682626875461175 19.849143231942282 55 16.16393442622951 22.057377049180328 46.385245901639344 15.39344262295082 56 19.867223998032948 20.809769691008935 44.47996065896238 14.843045651995737 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 3.0 7 3.0 8 3.0 9 3.0 10 4.0 11 5.0 12 16.5 13 36.0 14 44.0 15 55.0 16 66.0 17 84.0 18 102.0 19 108.0 20 114.0 21 133.5 22 167.5 23 182.0 24 266.0 25 350.0 26 463.0 27 576.0 28 665.5 29 719.0 30 683.0 31 694.0 32 705.0 33 637.0 34 569.0 35 499.5 36 430.0 37 358.5 38 274.0 39 261.0 40 223.5 41 186.0 42 174.5 43 163.0 44 159.5 45 156.0 46 162.5 47 195.0 48 221.0 49 239.0 50 257.0 51 290.0 52 323.0 53 353.0 54 460.5 55 538.0 56 579.5 57 621.0 58 624.5 59 628.0 60 617.0 61 606.0 62 625.5 63 593.0 64 541.0 65 490.0 66 439.0 67 373.0 68 307.0 69 254.5 70 202.0 71 199.5 72 158.0 73 119.0 74 112.5 75 106.0 76 84.0 77 62.0 78 40.0 79 15.0 80 12.0 81 10.0 82 8.0 83 4.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.03271180896303566 14 0.0 15 0.2616944717042853 16 0.057245665685312405 17 0.008177952240758915 18 0.0 19 0.07360157016683022 20 0.08995747464834805 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.008177952240758915 27 0.08177952240758914 28 0.10631337912986587 29 0.03271180896303566 30 0.03271180896303566 31 0.02453385672227674 32 0.13084723585214264 33 0.13902518809290154 34 0.06542361792607132 35 0.057245665685312405 36 0.04906771344455348 37 0.18809290153745503 38 0.2535165194635263 39 0.2616944717042853 40 0.8014393195943735 41 0.9895322211318286 42 0.13902518809290154 43 0.23716061498200852 44 0.5070330389270526 45 0.20444880601897283 46 0.21262675825973174 47 0.04088976120379457 48 0.15538109257441937 49 0.5152109911678115 50 0.14720314033366044 51 0.2616944717042853 52 0.13084723585214264 53 0.21262675825973174 54 0.2535165194635263 55 0.22898266274124962 56 0.2208047105004907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 12228.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.63559044815177 #Duplication Level Percentage of deduplicated Percentage of total 1 86.30882509870878 66.1432777232581 2 8.291537722761712 12.708537782139354 3 2.678476149823925 6.1579980372914624 4 0.9177248959556078 2.8132155708210664 5 0.5869170846227724 2.2489368662087013 6 0.38416390993490557 1.7664376840039255 7 0.13872585636538257 0.7441936539090612 8 0.1707395155266247 1.0467778868171411 9 0.09604097748372639 0.662414131501472 >10 0.41617756909614767 5.20117762512267 >50 0.010671219720414044 0.5070330389270526 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCAAGGGAATCTAAAGAGACAAAGTTAGGAATGGGAATCTTCTACAAACAAATT 62 0.5070330389270526 No Hit GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG 48 0.39254170755642787 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 33 0.2698724239450442 No Hit GCTCAAGGGGGACTCCAGGCTGCATCATATATCATAATTTATTTTAAAGAGAAAGT 29 0.23716061498200852 No Hit GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG 26 0.21262675825973174 No Hit GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGACACACGCAGGGATGAG 25 0.20444880601897283 No Hit GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGGGATGAG 25 0.20444880601897283 No Hit GCTCAAGGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG 20 0.16355904481517827 No Hit GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGGGATGAG 18 0.14720314033366044 No Hit GCTCAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 17 0.13902518809290154 No Hit GCTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCGGCCACACGCAGGGATGAG 17 0.13902518809290154 No Hit GCTCAAGGGGGACTCCAGGCTGCATCATATATCATAATTTCTTTTAAAGAGAAAGT 16 0.13084723585214264 No Hit GCTCAAGGGAAGTAACGCAGAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.13084723585214264 No Hit GCTCAAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 16 0.13084723585214264 No Hit GCTCAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.13084723585214264 No Hit GCTCAAGGGTGGTTCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.12266928361138371 No Hit GCTCAAGGGAGAGTGGTCACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.12266928361138371 No Hit GCTCAAGGGAAGCATGGATCACGCAGAGTCGCTTTTTTTTTTTTTTTTTTTTTTTT 15 0.12266928361138371 No Hit GCTCAAGGGAAGATGGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 15 0.12266928361138371 No Hit GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.12266928361138371 No Hit GCTCAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG 14 0.11449133137062481 No Hit GCTCAAGGGAGTGTTAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.11449133137062481 No Hit GCTCAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 14 0.11449133137062481 No Hit GCTCAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 14 0.11449133137062481 No Hit GCTCAAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.11449133137062481 No Hit GCTCAAGGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.10631337912986587 No Hit GCTCAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 13 0.10631337912986587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.02453385672227674 0.0 0.0 0.0 0.0 7 0.02453385672227674 0.0 0.0 0.0 0.0 8 0.02453385672227674 0.0 0.0 0.0 0.0 9 0.02453385672227674 0.0 0.0 0.0 0.0 10 0.03271180896303565 0.0 0.0 0.0 0.0 11 0.03271180896303565 0.0 0.0 0.0 0.0 12 0.04088976120379457 0.0 0.0 0.0 0.0 13 0.04088976120379457 0.0 0.0 0.0 0.0 14 0.04088976120379457 0.0 0.0 0.0 0.0 15 0.04088976120379457 0.0 0.0 0.0 0.0 16 0.04088976120379457 0.0 0.0 0.0 0.0 17 0.04906771344455348 0.0 0.0 0.0 0.0 18 0.04906771344455348 0.0 0.0 0.0 0.0 19 0.04906771344455348 0.0 0.0 0.0 0.0 20 0.0572456656853124 0.0 0.0 0.0 0.0 21 0.08177952240758914 0.0 0.0 0.0 0.0 22 0.08177952240758914 0.0 0.0 0.0 0.0 23 0.09813542688910697 0.0 0.0 0.0 0.0 24 0.09813542688910697 0.0 0.0 0.0 0.0 25 0.1144913313706248 0.0 0.0 0.0 0.0 26 0.1308472358521426 0.0 0.0 0.0 0.0 27 0.14720314033366044 0.0 0.0 0.0 0.0 28 0.15538109257441937 0.0 0.0 0.0 0.0 29 0.16355904481517827 0.0 0.0 0.0 0.0 30 0.16355904481517827 0.0 0.0 0.0 0.0 31 0.16355904481517827 0.0 0.0 0.0 0.0 32 0.16355904481517827 0.0 0.0 0.0 0.0 33 0.1717369970559372 0.0 0.0 0.0 0.0 34 0.1717369970559372 0.0 0.0 0.0 0.0 35 0.1717369970559372 0.0 0.0 0.0 0.0 36 0.1717369970559372 0.0 0.0 0.0 0.0 37 0.18809290153745503 0.0 0.0 0.0 0.0 38 0.18809290153745503 0.0 0.0 0.0 0.0 39 0.18809290153745503 0.0 0.0 0.0 0.0 40 0.18809290153745503 0.0 0.0 0.0 0.0 41 0.18809290153745503 0.0 0.0 0.0 0.0 42 0.18809290153745503 0.0 0.0 0.0 0.0 43 0.18809290153745503 0.0 0.0 0.0 0.0 44 0.18809290153745503 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGATG 15 0.0081609925 50.11111 8 CTACAAA 20 3.977526E-4 50.11111 44 ATGGGAA 15 0.0081609925 50.11111 33 ACAAACA 20 3.977526E-4 50.11111 46 AACAAAT 20 3.977526E-4 50.11111 49 AATCTAA 20 3.977526E-4 50.11111 10 CACACGC 15 0.0081609925 50.11111 41 GATACTC 15 0.0081609925 50.11111 9 ATCTAAA 20 3.977526E-4 50.11111 11 GAAGCAT 20 3.977526E-4 50.11111 9 TCGGACA 15 0.0081609925 50.11111 36 TCTACAA 20 3.977526E-4 50.11111 43 GGGAGAT 25 1.941288E-5 50.11111 7 GGATACT 15 0.0081609925 50.11111 8 AACCAGC 15 0.0081609925 50.11111 33 AGCGGTG 15 0.0081609925 50.11111 47 TCTAAAG 20 3.977526E-4 50.11111 12 TACAAAC 25 1.941288E-5 50.11111 45 GGCTTTT 25 1.941288E-5 50.11111 9 GAATCTA 20 3.977526E-4 50.11111 9 >>END_MODULE