FastQCFastQC Report
Fri 17 Jun 2016
SRR1526202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54746
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA810.14795601505132797No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.1424761626420195No Hit
AACCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG660.12055675300478574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGC252.0018244E-550.24700537
GATTTGC204.0488143E-450.24700537
TCAAGAG150.00825629450.20073735
CGTTCGC150.00828642450.15455647
GTTCGCG150.00828642450.15455648
ACAAAAC252.0238005E-550.15455248
TCGTTCG150.00831663650.10845646
GTCTTAA402.57387E-950.06244713
AACCTGC150.00834692950.06244342
TCAAAGC150.00834692950.06244314
CCCCTGT150.00834692950.06244342
GGGCACC150.00837730550.0165189
GGTCCGG150.00837730550.01651810
CATCTCA150.00837730550.01651811
GATGTAT150.00837730550.0165189
GGATTCT150.00837730550.0165189
GCAGTTA150.00837730550.01651812
GCATGGA150.00837730550.0165189
GAATCCG150.00837730550.01651810
CAAAGCA150.00837730550.01651815
Produced by FastQC (version 0.11.5)