##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526202_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54746 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.45307419720162 39.0 38.0 40.0 33.0 40.0 2 37.17676177254959 39.0 38.0 40.0 33.0 40.0 3 36.17504475212801 39.0 36.0 40.0 28.0 40.0 4 36.4698425455741 39.0 38.0 40.0 29.0 40.0 5 36.2075768092646 39.0 36.0 40.0 28.0 40.0 6 36.3636795382311 39.0 37.0 40.0 30.0 40.0 7 36.26193694516494 39.0 36.0 40.0 30.0 40.0 8 36.29814050341577 39.0 36.0 40.0 30.0 40.0 9 36.29141855112702 39.0 36.0 40.0 30.0 40.0 10 35.87418258868228 39.0 35.0 40.0 28.0 40.0 11 35.21519380411354 38.0 34.0 39.0 27.0 40.0 12 33.0562780842436 38.0 31.0 39.0 17.0 40.0 13 32.836499470280934 38.0 30.0 39.0 17.0 40.0 14 33.168596792459724 38.0 31.0 39.0 18.0 40.0 15 33.10280203119863 38.0 31.0 39.0 18.0 40.0 16 33.25448434588829 38.0 31.0 39.0 19.0 40.0 17 33.36035509443612 38.0 31.0 39.0 20.0 40.0 18 32.785555109049064 37.0 30.0 39.0 17.0 40.0 19 33.746264567274324 38.0 31.0 39.0 23.0 40.0 20 33.54095276367223 37.0 31.0 39.0 22.0 40.0 21 34.4073356957586 38.0 33.0 39.0 24.0 40.0 22 34.15789281408687 38.0 33.0 39.0 23.0 40.0 23 34.468454316297084 38.0 33.0 39.0 24.0 40.0 24 34.17458809776057 38.0 33.0 39.0 23.0 40.0 25 34.31980418660724 38.0 33.0 39.0 24.0 40.0 26 34.96863697804406 38.0 35.0 39.0 27.0 40.0 27 34.82205092609506 38.0 35.0 39.0 26.0 40.0 28 34.803930880794944 38.0 35.0 39.0 26.0 40.0 29 34.72235414459504 38.0 34.0 39.0 26.0 40.0 30 34.59288349833778 38.0 34.0 39.0 25.0 40.0 31 34.96485587988163 38.0 35.0 39.0 27.0 40.0 32 34.57056223285719 38.0 34.0 39.0 26.0 40.0 33 34.73209001570891 38.0 35.0 39.0 27.0 40.0 34 34.65412998209915 38.0 35.0 39.0 27.0 40.0 35 34.521060899426445 38.0 34.0 39.0 26.0 40.0 36 33.67793080773024 37.0 33.0 39.0 25.0 40.0 37 33.458097395243485 36.0 32.0 39.0 24.0 40.0 38 33.178497059145876 36.0 32.0 39.0 23.0 40.0 39 32.98160596207942 36.0 32.0 39.0 22.0 40.0 40 29.51607423373397 35.0 24.0 39.0 6.0 40.0 41 26.04389361779856 31.0 14.0 38.0 4.0 39.0 42 30.36552442187557 35.0 29.0 38.0 4.0 39.0 43 28.689365433091 33.0 25.0 38.0 4.0 39.0 44 28.704471559565995 33.0 25.0 38.0 4.0 39.0 45 29.822690242209475 34.0 28.0 38.0 4.0 39.0 46 29.457732071749533 35.0 27.0 38.0 4.0 40.0 47 29.040550907828884 35.0 26.0 38.0 4.0 40.0 48 29.13955357469039 35.0 26.0 38.0 4.0 40.0 49 26.394951229313556 33.0 12.0 38.0 4.0 40.0 50 28.732765864172723 34.0 25.0 38.0 4.0 40.0 51 29.019051620209694 35.0 26.0 39.0 4.0 40.0 52 28.698133196945896 35.0 25.0 39.0 4.0 40.0 53 28.31204106236072 35.0 23.0 39.0 4.0 40.0 54 27.332754904467905 34.0 15.0 39.0 4.0 40.0 55 27.722226281372155 34.0 20.0 39.0 4.0 40.0 56 26.902148102144448 34.0 15.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.500611347695525 1 2 2.1754436302935716 1 3 3.3832171579019317 1 4 2.900055231511253 1 5 3.2263358235980277 1 6 3.120198266764632 1 7 2.8498518291610537 1 8 3.1562853227570358 1 9 3.19796718679423 1 10 3.1230672972994853 1 11 0.46792267000215304 1 12 -11.049188306648961 1 13 -4.213321588447904 1 14 -2.657752302531577 1 15 -3.6798266838508304 1 16 -21.33190570758223 1 17 -2.9876319660790784 1 18 -10.920369690828277 1 19 -3.9362267302898672 1 20 -3.329739543792769 1 21 -2.6715770680633533 1 22 -0.7178497691162136 1 23 -1.05402072160674 1 24 -1.5514599486014369 1 25 -0.8261134877280014 1 26 3.066680387684805 1 27 3.20429020209189 1 28 3.018874228980387 1 29 3.535216816703077 1 30 3.518488673930591 1 31 3.3078293654626663 1 32 3.723364069423525 1 33 3.1321951696733734 1 34 3.381332146069333 1 35 3.1697170458673014 1 36 3.5553452493016664 1 37 4.137865217883672 1 38 3.9830250032196446 1 39 4.09303875774409 1 40 6.852118606608855 1 41 -8.118631850560615 1 42 -7.761573817617048 1 43 -16.0223445243698 1 44 -21.387818131439317 1 45 -10.684647965221654 1 46 -17.772400359786555 1 47 -22.55134390601883 1 48 -14.436688345679384 1 49 -19.217287385880336 1 50 -14.177557509005656 1 51 -11.219384824955107 1 52 -10.794925681324212 1 53 -10.943751377278401 1 54 -10.106950653604613 1 55 -10.51301446966996 1 56 -5.91938270207795 2 1 2.138846641813167 2 2 2.5158848067641557 2 3 3.9015995108431056 2 4 3.6074081726877267 2 5 3.471188764774496 2 6 3.8363747373528625 2 7 3.5755871232787015 2 8 3.318785322757037 2 9 3.5810554220883475 2 10 3.760567297299488 2 11 3.0370403170609777 2 12 -0.7521294831195497 2 13 1.3271195880226827 2 14 4.833424168056656 2 15 4.720908610266818 2 16 3.2085354688883605 2 17 4.084426857450332 2 18 -0.5784579261223932 2 19 3.3814203285336646 2 20 3.385701632677815 2 21 4.446805284877826 2 22 4.6314149367661415 2 23 3.879802807805028 2 24 2.9044224043397406 2 25 4.420210041683767 2 26 3.565209799449505 2 27 3.781496084444832 2 28 3.0710801113333304 2 29 3.3528638755266087 2 30 3.658194556283533 2 31 3.0820940713450184 2 32 3.1932170105999944 2 33 2.0182245814380764 2 34 1.9188321460693345 2 35 3.4969229282202434 2 36 4.028874661066368 2 37 3.877571100236615 2 38 3.149201473807878 2 39 4.096715228332322 2 40 5.8977068419029735 2 41 -4.686278909384143 2 42 -6.404220876440576 2 43 -13.16278570084039 2 44 -14.142229896145198 2 45 -6.786853847574594 2 46 -10.879753300963028 2 47 -17.624138023665886 2 48 -8.308747169208797 2 49 -12.610669738821514 2 50 -8.001086920770362 2 51 -3.326002472013929 2 52 -2.966984504853624 2 53 -3.003310200807814 2 54 -2.0569506536046127 2 55 -1.8600732931993704 2 56 0.873999650863226 3 1 2.2628972747245655 3 2 3.0821822751185906 3 3 4.240998245020322 3 4 4.022914501801651 3 5 3.7795748407238676 3 6 3.972767142416153 3 7 3.6975807941647716 3 8 3.893627094908936 3 9 3.551466814493409 3 10 3.955345778312143 3 11 4.3031795575673115 3 12 7.104199630804505 3 13 5.149113258908756 3 14 5.156841889575649 3 15 5.623598483684539 3 16 5.995719013192161 3 17 6.1341104017541355 3 18 6.393852200459886 3 19 5.770027923470373 3 20 5.418771252930981 3 21 5.2020268038651665 3 22 5.092649113981331 3 23 5.210499010336676 3 24 5.243029999276452 3 25 5.008026497379966 3 26 3.68451359691786 3 27 3.8519074768498953 3 28 3.7899092252573823 3 29 3.516787926159516 3 30 3.647118606916443 3 31 2.6531383751424897 3 32 3.8747043523721487 3 33 4.041167619412761 3 34 2.837028348600981 3 35 2.6749292573341705 3 36 4.733305040813207 3 37 4.272824264793577 3 38 4.67103691684585 3 39 4.752569658712069 3 40 8.44928912038398 3 41 9.4187843817551 3 42 5.690399376723981 3 43 0.24496746371657352 3 44 3.013149850690244 3 45 5.879760076476035 3 46 3.9971454332141896 3 47 -1.014486124931711 3 48 4.027012324462092 3 49 6.636956843456968 3 50 4.090685231128372 3 51 5.853586135581008 3 52 5.440926887551438 3 53 5.336404989065603 3 54 6.016467067914373 3 55 5.890559618193034 3 56 5.4953604103568985 4 1 2.1109985405473495 4 2 2.613827844738843 4 3 3.595428624767159 4 4 3.3140537423079763 4 5 3.3238786381922196 4 6 3.1879570158338737 4 7 3.178593452392626 4 8 3.3746397531367833 4 9 3.52615035879721 4 10 2.740155904894422 4 11 4.252546646174906 4 12 6.370022415614631 4 13 6.022530980427746 4 14 5.346715307297163 4 15 5.851446584950359 4 16 5.90711141825545 4 17 5.640439515678182 4 18 6.0141053650168494 4 19 5.023192480432392 4 20 4.7858598605259175 4 21 3.100760981080356 4 22 4.320497215247151 4 23 3.9826509090708484 4 24 3.964548986618219 4 25 4.083975864468577 4 26 2.773121191854571 4 27 2.0924138059638224 4 28 3.156997832852319 4 29 3.6940031160329383 4 30 3.102814809448091 4 31 2.4252902738766693 4 32 3.1785018207265807 4 33 2.9905347080203555 4 34 2.267408095436423 4 35 2.168600143410117 4 36 3.2522923825853596 4 37 3.247507809097371 4 38 3.000150840896481 4 39 2.9171266207373847 4 40 5.904985322915628 4 41 10.165619824793072 4 42 6.2220449463442336 4 43 7.7892712611849255 4 44 6.380238458285181 4 45 5.9683676714127465 4 46 4.971828977517983 4 47 4.960197419372086 4 48 5.21688574218361 4 49 7.042020134596207 4 50 2.090685231128372 4 51 1.2206747431759446 4 52 1.4915597989438432 4 53 1.133873343495985 4 54 1.7633025109523501 4 55 0.8905596181930342 4 56 0.81181610655943 5 1 2.3245609275274575 5 2 2.649813378192732 5 3 3.717670939414532 5 4 3.6824081726877225 5 5 3.6944030504887877 5 6 3.647089023067153 5 7 3.6952299804215585 5 8 3.7777138941856094 5 9 3.8185554220883446 5 10 4.058781583013769 5 11 4.740611745632407 5 12 7.25501337402331 5 13 6.861048159451251 5 14 6.054852739485231 5 15 6.865551467409674 5 16 5.9924640403169285 5 17 5.7451411431646235 5 18 5.909042073877604 5 19 5.024277471390803 5 20 4.816058775534962 5 21 4.584305284877821 5 22 4.351057793909 5 23 4.5262313792336 5 24 4.7972795471968865 5 25 4.6541386131123375 5 26 3.468781228020937 5 27 3.5761389415876863 5 28 3.9550086827618998 5 29 3.845721018383749 5 30 3.1903374134263913 5 31 2.841022642773595 5 32 3.3128598677428513 5 33 3.3342960100095027 5 34 2.2099035746407623 5 35 2.862994356791674 5 36 5.250303232494943 5 37 5.009713957379468 5 38 4.195630045236449 5 39 5.584215228332319 5 40 9.122706841902968 5 41 10.828006804901573 5 42 7.206493409273712 5 43 8.081857156302465 5 44 7.639912960997659 5 45 5.7363604381396875 5 46 5.888103841894118 5 47 6.870504833476971 5 48 6.709109973648349 5 49 9.226830261178485 5 50 6.734627364943925 5 51 6.2722118137003555 5 52 5.850872638003519 5 53 6.502046942049329 5 54 6.843049346395389 5 55 6.1827838496577705 5 56 5.915071079434657 6 1 2.58884664181317 6 2 2.9757532278167886 6 3 4.102257405579948 6 4 3.900829225319306 6 5 4.148162448985026 6 6 4.087690526826549 6 7 4.003876596962911 6 8 3.9589169017044057 6 9 3.839608053667291 6 10 3.833593613088958 6 11 4.340987685482034 6 12 7.1768178853015065 6 13 6.284356430127943 6 14 6.875529431214556 6 15 6.7768296628984 6 16 6.599982837309415 6 17 6.106795278502965 6 18 6.477463126509189 6 19 5.918262433796819 6 20 5.9837279484672905 6 21 4.165884232246242 6 22 5.351809673608244 6 23 5.12322386043661 6 24 4.7596855622344805 6 25 4.877446883789027 6 26 3.5481045362916106 6 27 2.771627663392202 6 28 2.4677906376491165 6 29 4.402863875526606 6 30 4.2437208720730055 6 31 3.351173018713439 6 32 4.079401221126311 6 33 2.495198265648604 6 34 2.933963725016703 6 35 1.4738966124307709 6 36 2.5499272926453145 6 37 2.7052026791839836 6 38 2.8978856843341916 6 39 3.5868468072796915 6 40 6.993759473481916 6 41 4.526878985352699 6 42 5.21288438671732 6 43 6.308924825475398 6 44 6.490664840696905 6 45 4.576961941899089 6 46 5.713667751668556 6 47 6.173888292123586 6 48 5.88993704131752 6 49 8.274198682231116 6 50 5.86733413186122 6 51 4.631234370091331 6 52 4.6501207583042685 6 53 5.080242430771136 6 54 5.090417767448017 6 55 4.984663548905893 6 56 4.29965754560007 7 1 2.2120860784328897 7 2 2.848807341975423 7 3 4.3707896516881775 7 4 3.726070144518708 7 5 4.094604257732243 7 6 3.9064451598880723 7 7 3.966256137363203 7 8 3.7654402523345 7 9 3.7636258446235544 7 10 4.1336306775811735 7 11 4.462744542413091 7 12 7.118997277443835 7 13 7.093844940135362 7 14 6.734128393408771 7 15 6.69009875111189 7 16 6.232303074522161 7 17 5.7087226320982225 7 18 6.665380102046623 7 19 5.385645680646341 7 20 5.4774269847904975 7 21 4.7279672567088085 7 22 4.91222479592107 7 23 4.670295765551508 7 24 4.830478742367909 7 25 4.785526943092215 7 26 3.5064069825481 7 27 4.092235521064552 7 28 3.015974477530513 7 29 3.5014554248223817 7 30 4.181081880227197 7 31 3.8146644938802297 7 32 3.848674757078868 7 33 3.586006271578924 7 34 3.789782850294685 7 35 3.9273806746991156 7 36 4.407043675150874 7 37 4.807500677701405 7 38 3.994624009019141 7 39 4.594074383261898 7 40 7.843833602466354 7 41 0.11231263991163232 7 42 5.782750954545335 7 43 7.483869228737074 7 44 6.582770103854802 7 45 5.043603898904276 7 46 6.253697403262326 7 47 6.283784511545377 7 48 6.123999309664445 7 49 9.193027444277078 7 50 7.044687727116958 7 51 7.4583285139015665 7 52 7.202381692329471 7 53 5.792992616093596 7 54 6.931781740761586 7 55 5.741335157504853 7 56 5.6879081015674515 8 1 2.0773081802747058 8 2 2.6860771144564666 8 3 3.6275610493046457 8 4 3.6285620188415706 8 5 3.7702272263129615 8 6 3.5536824296605545 8 7 3.5227025078940812 8 8 3.6447468612185716 8 9 3.657016960549882 8 10 3.296144220376405 8 11 4.335117240137905 8 12 7.197870516880453 8 13 6.851158049561143 8 14 6.34496262959512 8 15 6.685331687189894 8 16 6.144112391965283 8 17 5.205580703604184 8 18 5.639811304646837 8 19 4.635266482379812 8 20 5.32704778652397 8 21 4.13815143872398 8 22 4.424684167535375 8 23 4.477879730881952 8 24 4.4467300966474355 8 25 3.7980946570683827 8 26 3.155594414834127 8 27 3.418996084444835 8 28 3.0374262651794837 8 29 3.9720946447573766 8 30 3.548579171668152 8 31 1.723440225191176 8 32 3.1095631644461506 8 33 3.0826476583611537 8 34 2.4121013768385637 8 35 3.1421152359125486 8 36 3.578874661066372 8 37 3.4679557156212297 8 38 3.708816858423262 8 39 3.422676766793863 8 40 7.738091457287588 8 41 -3.817048140153375 8 42 5.474625277405579 8 43 6.317021991467303 8 44 6.567385488470183 8 45 4.519876921656174 8 46 5.383708237498514 8 47 5.382592745564885 8 48 5.1047143692527435 8 49 7.949907184255409 8 50 3.6412207715373306 8 51 4.3788052202937635 8 52 4.808015495146375 8 53 5.32842056842296 8 54 6.281510884856928 8 55 5.363003629877554 8 56 5.878807343170919 9 1 1.985905465342583 9 2 2.6313259832347455 9 3 3.6185112755489897 9 4 3.547114055040666 9 5 3.0792770000686147 9 6 3.634904149117567 9 7 3.0263224173963437 9 8 3.5239323815805648 9 9 3.212673069147172 9 10 3.5495378855347823 9 11 3.703216787649211 9 12 6.40669404629222 9 13 5.933737235081509 9 14 6.2687182857037165 9 15 6.084879198502115 9 16 5.2857413512413025 9 17 5.556485680979748 9 18 5.520806779759962 9 19 4.387302681474836 9 20 4.596731044442521 9 21 3.0931288142895923 9 22 3.6350914073543734 9 23 3.740096925452086 9 24 4.595598874927973 9 25 3.4385923946249406 9 26 3.213739211214211 9 27 2.983701966797774 9 28 2.636521287803916 9 29 3.344040346114838 9 30 3.400841615107062 9 31 3.2784176007567893 9 32 3.120422892952938 9 33 3.4557245814380764 9 34 3.204861557834036 9 35 3.2138346929261274 9 36 4.158286425772253 9 37 4.034924041413085 9 38 4.4094955914549345 9 39 5.004803463626438 9 40 8.087412724255913 9 41 5.2490152082629145 9 42 6.54724971179472 9 43 8.271773122689023 9 44 7.89159363326657 9 45 5.888881446543049 9 46 7.7864231696252055 9 47 7.581009035157642 9 48 8.019194007261792 9 49 10.488594967060841 9 50 7.76361896158258 9 51 7.986497527986071 9 52 7.46978020102873 9 53 7.409189799192188 9 54 8.745990522865974 9 55 8.7222796479771 9 56 8.742382003804401 10 1 2.1359480910885296 10 2 2.7204137922714082 10 3 3.79924443837934 10 4 3.8693646944268565 10 5 4.007239489412179 10 6 3.945432708367356 10 7 3.6917103116844956 10 8 3.8772998155106606 10 9 3.9765264365810964 10 10 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2.712462349680159 115 6 2.9481671901830495 115 7 2.541860708184359 115 8 2.5666626812475997 115 9 2.597800705107211 115 10 3.0711805048466516 115 11 0.3893988076270176 115 12 -10.062506841610116 115 13 -5.0443426761282595 115 14 -8.056198473452774 115 15 -10.082157427469031 115 16 -7.658209814130508 115 17 -1.5575542746251365 115 18 -2.221146605367675 115 19 -2.960089105428601 115 20 -2.472081386190105 115 21 3.1427958509155616 115 22 3.5901413518604812 115 23 3.7359348832767267 115 24 3.267629951509555 115 25 3.8128987209290486 115 26 2.3666248937891297 115 27 2.5020149523693576 115 28 1.6420706773710663 115 29 1.8745619887341505 115 30 1.9860247449627764 115 31 1.6299714298355852 115 32 1.2236415389018802 115 33 1.9415736380418522 115 34 1.4002000705976343 115 35 0.8495172678428844 115 36 2.869440698802215 115 37 2.011061666274351 115 38 2.6789184549399536 115 39 2.718177492483264 115 40 5.681197407940708 115 41 4.7684380717479335 115 42 1.0641753499745157 115 43 -7.903587587632842 115 44 -5.473833669730105 115 45 -0.2040708287066728 115 46 -4.437772168887555 115 47 -3.918713495363999 115 48 -4.173369810718228 115 49 -12.83354709731208 115 50 -5.251086920770362 115 51 -4.588974170127138 115 52 -4.475003372778154 115 53 -5.444583785713473 115 54 -9.536195936623482 115 55 -6.877054425274842 115 56 -4.609726764231112 116 1 1.5761347774063879 116 2 2.1343170101539926 116 3 3.2585910362668358 116 4 3.002747155738568 116 5 3.3120785952829763 116 6 2.993578127183369 116 7 3.1414769537871763 116 8 3.058827695638392 116 9 2.6643181339527544 116 10 3.220524924418129 116 11 0.5883115035016573 116 12 -13.029248127187344 116 13 -6.695126174689182 116 14 -1.5979317641467325 116 15 -2.879726982953521 116 16 -1.419642497213335 116 17 -0.2134544984818696 116 18 -7.509601993919002 116 19 -1.0643423833307466 116 20 -2.057306841898452 116 21 3.2249832509795198 116 22 2.821033580833941 116 23 2.4836163671270626 116 24 3.4595071501024535 116 25 3.253896482361732 116 26 3.1232606469071342 116 27 2.863063881054998 116 28 1.809003840146886 116 29 2.979135061967284 116 30 2.3128555732326888 116 31 2.1221364442263777 116 32 2.4913102309389785 116 33 2.1379279712685815 116 34 2.194891468103229 116 35 1.9767958095761742 116 36 3.344976355981622 116 37 3.737316862948475 116 38 3.2292862195705894 116 39 4.046927092739104 116 40 6.932876333428396 116 41 5.8611787177345 116 42 0.6462028523729835 116 43 -8.476980616094629 116 44 -7.298585828348589 116 45 0.1438664914084562 116 46 -2.697126182318957 116 47 -1.871383786377752 116 48 -3.223577677683373 116 49 -5.851135840516431 116 50 -2.9121038699229054 116 51 0.1475144771386141 116 52 0.011405325654848752 116 53 -1.7052169804688297 116 54 -4.484069297672409 116 55 0.3822995881565596 116 56 1.5322623627276357 117 1 0.8969861766968918 117 2 1.4528034114153243 117 3 2.3449134643314764 117 4 2.107989568036558 117 5 2.3179910903558962 117 6 2.5962584582830956 117 7 2.263668518627533 117 8 2.4179132297337773 117 9 1.616229840692995 117 10 1.8846952042762268 117 11 -1.9647038689855307 117 12 -9.006780645910247 117 13 -7.377822272442433 117 14 -11.949133971478226 117 15 -10.19624255252388 117 16 -16.281290112506987 117 17 -12.980108026270596 117 18 -15.135143972634022 117 19 -6.620905252861686 117 20 -7.55237976267102 117 21 0.15639830813363886 117 22 0.10089168095218781 117 23 0.21991908687479622 117 24 -0.12464736310212032 117 25 0.16875655331167394 117 26 1.419279566891369 117 27 1.6608565495611103 117 28 1.2138126694728655 117 29 0.7284452708754401 117 30 1.8355201376788841 117 31 1.4347103504147896 117 32 1.7045542199023203 117 33 0.5313059767869106 117 34 1.52086702979026 117 35 0.6437252538016409 117 36 2.2160839633919522 117 37 2.140943193259872 117 38 1.8776898459009033 117 39 3.028401274843951 117 40 6.648288237251805 117 41 2.827674578987949 117 42 -3.1815464578359247 117 43 -9.910169421770622 117 44 -11.67304384963357 117 45 -2.426098033621109 117 46 -3.6431253939862813 117 47 -4.344777558549609 117 48 -4.415142518046004 117 49 -8.894099971379653 117 50 -3.4371334323982694 117 51 -1.3739675882929987 117 52 -2.3315193885745558 117 53 -3.3524381077845575 117 54 -8.856950653604613 117 55 -2.694957014129603 117 56 -2.841407325880958 118 1 0.9846799751465056 118 2 1.4867181400974943 118 3 2.7765995108431056 118 4 2.449074839354388 118 5 2.400355431441163 118 6 2.728041404019528 118 7 2.5214204566120344 118 8 2.5937853227570358 118 9 2.356055422088346 118 10 3.018900630632821 118 11 -1.1546263496056852 118 12 -8.67296281645288 118 13 -10.422880411977317 118 14 -10.237409165276674 118 15 -4.674924723066514 118 16 -12.958131197778307 118 17 -11.465573142549665 118 18 -13.245124592789061 118 19 -3.672746338133006 118 20 -4.08096503398885 118 21 0.8468052848778242 118 22 1.9022482700994772 118 23 1.6506361411383637 118 24 1.446089071006405 118 25 2.1077100416837666 118 26 2.7235431327828437 118 27 3.0731627511114965 118 28 2.971080111333329 118 29 2.88203054219327 118 30 2.9290278896168687 118 31 2.552927404678357 118 32 -0.7901163227333399 118 33 2.289057914771412 118 34 2.131332146069333 118 35 2.955256261553579 118 36 3.8580413277330337 118 37 3.569237766903278 118 38 4.4867014738078765 118 39 3.6592152283323216 118 40 8.32270684190297 118 41 3.9720544239491886 118 42 -1.795887543107245 118 43 -11.354452367507058 118 44 -8.458896562811866 118 45 -4.011853847574596 118 46 -9.529753300963026 118 47 -10.661638023665887 118 48 -9.750413835875463 118 49 -11.881503072154848 118 50 -7.29275358743703 118 51 -6.263502472013929 118 52 -6.233651171520293 118 53 -6.424143534141145 118 54 -12.83611732027128 118 55 -6.493406626532703 118 56 -4.8801670158034405 119 1 0.6602752132417393 119 2 1.1355276639070127 119 3 2.3926709394145362 119 4 1.8538367441162933 119 5 2.4122601933459293 119 6 2.061374737352864 119 7 2.0809442661358446 119 8 2.26342817989989 119 9 1.793555422088346 119 10 2.53378158301377 119 11 -3.7736739686533056 119 12 -10.652129483119548 119 13 -12.199666126263033 119 14 -9.788004403371914 119 15 -8.341591389733182 119 16 -14.639678816825924 119 17 -14.037001713978238 119 18 -17.355243640408105 119 19 -7.497151100037765 119 20 -9.191084081607897 119 21 -1.1978375722650298 119 22 -0.020370777519573835 119 23 -0.030911477909256746 119 24 0.49370811862545594 119 25 0.33985289882662073 119 26 1.8366383708780774 119 27 1.8082817987305475 119 28 1.7478658256190442 119 29 1.7064353040980365 119 30 2.265337413426387 119 31 0.7731654999164519 119 32 -4.5371401322571465 119 33 0.9378674385809305 119 34 0.9527607174979025 119 35 0.13085149964881282 119 36 1.289588946780654 119 37 1.6347139573794678 119 38 1.7099157595221612 119 39 2.0163580854751757 119 40 5.51913541333154 119 41 -1.8969931950984282 119 42 -7.129220876440577 119 43 -13.607428557983248 119 44 -11.43508703900234 119 45 -9.502925276146023 119 46 -18.440467586677315 119 47 -16.733066595094456 119 48 -15.815890026351653 119 49 -16.226741167392944 119 50 -14.161801206484649 119 51 -13.727788186299643 119 52 -13.584841647710768 119 53 -13.019381629379241 119 54 -15.124807796461756 119 55 -12.892216150342229 119 56 -9.927786063422486 120 1 0.6361439391104682 120 2 1.0756821040614568 120 3 2.197207618951211 120 4 1.8905162807958291 120 5 2.027607683693418 120 6 1.682996358974485 120 7 1.7488979340895128 120 8 1.825204241675955 120 9 1.9996365031694268 120 10 1.9136078378400256 120 11 -2.0690407640201016 120 12 -9.321048402038468 120 13 -7.177947979544886 120 14 -15.743602858970368 120 15 -11.915915714057505 120 16 -17.114099666246773 120 17 -13.651383953360476 120 18 -15.445011980176448 120 19 -7.075336428223096 120 20 -13.686257826781642 120 21 -3.5940730935005547 120 22 -2.6979769551257498 120 23 -1.380332327330109 120 24 -7.061793811876477 120 25 -1.5578304988567737 120 26 2.1131827724224834 120 27 2.597374462823211 120 28 0.6619584897117079 120 29 1.2407017133644445 120 30 2.4250864481754277 120 31 -0.21959511784416463 120 32 -11.103742448859464 120 33 1.2597786354921325 120 34 -0.45650569176850553 120 35 0.38036887416618725 120 36 1.9464422286339342 120 37 1.1120305596960733 120 38 0.3836609332673362 120 39 0.5815125256296199 120 40 4.5118960310921565 120 41 -1.7160086391138734 120 42 -5.635977633197335 120 43 -11.83474516029985 120 44 -8.633446112361415 120 45 -6.503407901628648 120 46 -17.641239787449514 120 47 -16.11096234799021 120 48 -14.42834176380339 120 49 -17.578575144226917 120 50 -13.413249082932523 120 51 -11.32431328282474 120 52 -11.759552072421194 120 53 -11.543512903510516 120 54 -14.938031734685694 120 55 -12.160073293199371 120 56 -10.132419268055692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 957.0 5 138.0 6 210.0 7 42.0 8 85.0 9 77.0 10 71.0 11 71.0 12 92.0 13 100.0 14 120.0 15 170.0 16 187.0 17 244.0 18 272.0 19 333.0 20 400.0 21 527.0 22 639.0 23 800.0 24 939.0 25 1083.0 26 1173.0 27 1258.0 28 1484.0 29 1682.0 30 1994.0 31 2378.0 32 2661.0 33 3002.0 34 3535.0 35 4271.0 36 5320.0 37 7052.0 38 6806.0 39 4563.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.53421254520877 0.3945493734702078 0.06393161144193184 0.007306469879077924 8 99.59997077412048 0.33061776202827603 0.04383881927446754 0.025572644576772732 9 98.08205165674204 1.3005516384758704 0.3543637891352793 0.26303291564680525 10 44.574946114784645 38.799181675373546 8.280056990465056 8.345815219376759 11 32.491871552259525 32.570416103459614 18.213202791071495 16.724509553209366 12 37.88221970554926 16.14729843276221 21.106564863186353 24.863916998502173 13 24.43681143003307 17.320446531343066 26.050097747245722 32.192644291378144 14 24.774396258814075 20.651419385480985 27.527309926564612 27.04687442914033 15 25.808754874498817 19.615166327969096 28.29497812196774 26.281100675564346 16 27.97609255908318 20.966533238288463 26.00208367604321 25.05529052658515 17 27.95820699229167 23.280239652212035 22.505753845029773 26.255799510466517 18 26.28502538998283 24.743360245497385 22.745040733569578 26.226573630950206 19 24.971203949172686 27.53633787366304 21.842947252948168 25.649510924216106 20 26.870474658085275 24.908578951217727 22.17691801360345 26.044028377093543 21 26.904248712234686 23.50126036605414 24.120483688305995 25.47400723340518 22 27.138055748365176 23.366090673291197 24.30862528769225 25.18722829065137 23 27.79381142001242 24.304972052752714 24.38168998648303 23.519526540751837 24 25.47400723340518 24.41456910093888 25.62196324845651 24.489460417199428 25 25.448434588828405 24.337851167208562 26.233880100829282 23.979834143133743 26 24.15323906609668 22.86893931089919 30.571084073221527 22.4067375497826 27 23.522313838053275 19.69943506956506 32.46430334387627 24.313947748505402 28 22.29151426481346 21.72457937088515 33.52962692026335 22.45427944403804 29 22.647128683926844 20.955217335696403 35.08249438161188 21.31515959876487 30 19.66488205090723 20.324519889634 38.75417983810551 21.25641822135326 31 20.196981325147096 19.3472937908855 40.86174761539305 19.593977268574353 32 22.15803137226224 17.63501407729716 42.379977330066914 17.82697722037369 33 20.503860368107137 18.383402246697646 43.929525412565404 17.18321197262981 34 17.980444119528467 18.47939321940967 46.97432148405373 16.565841177008135 35 17.996747135469015 20.48390928528353 47.8024889896018 13.716854589645658 36 18.837825491091824 20.049337597076292 46.04659661946094 15.066240292370944 37 18.556059718969557 19.573331381733023 46.14680913348946 15.723799765807964 38 18.654064549217864 20.41249954207422 46.37505953035132 14.558376378356597 39 18.77676512572746 19.700962629479154 46.05248709783683 15.469785146956552 40 14.10643309288974 15.287796260856764 40.75518916531724 29.850581480936256 41 12.752581120943955 15.156710914454278 38.567477876106196 33.52323008849557 42 16.26184292351026 19.33277243296631 46.09503603175184 18.31034861177159 43 15.583464538098202 16.67459997803083 45.8313500054923 21.910585478378675 44 14.67024089306698 16.92494124559342 44.73413631022326 23.670681551116335 45 17.09459583112201 19.8543271782296 47.36013762055524 15.69093937009315 46 17.966176147594993 19.47983014861996 46.74939600263562 15.804597701149426 47 15.455874291978805 19.27827516901151 46.976064315731776 18.28978622327791 48 17.02123767538003 20.15292589679331 47.48019829147383 15.345638136352827 49 14.443138407287687 16.865633264766384 40.47715251248898 28.21407581545695 50 15.011154591668799 21.383900815565227 46.86574260322569 16.739201989540284 51 17.051678971183616 21.629692051220985 45.15360800190521 16.165020975690183 52 16.83735049925021 22.312644014483745 44.740499615961376 16.109505870304673 53 16.199901220114512 22.51815537710136 44.961311211517 16.320632191267126 54 15.082213351887793 21.998022485077087 42.77474640202146 20.14501776101366 55 16.621213507824656 22.26045575180745 44.26649583600256 16.851834904365333 56 17.34572531513566 23.564280356391446 42.541941857699555 16.54805247077334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 8.5 9 14.0 10 33.5 11 53.0 12 107.0 13 231.0 14 301.0 15 409.0 16 517.0 17 604.0 18 691.0 19 740.5 20 790.0 21 982.5 22 1466.5 23 1758.0 24 2062.0 25 2366.0 26 2633.5 27 2901.0 28 3115.5 29 3215.0 30 3100.0 31 2678.0 32 2256.0 33 1925.5 34 1595.0 35 1418.0 36 1241.0 37 1130.0 38 930.0 39 841.0 40 845.5 41 850.0 42 872.5 43 895.0 44 947.5 45 1000.0 46 1124.0 47 1342.5 48 1437.0 49 1502.5 50 1568.0 51 1653.5 52 1739.0 53 1851.5 54 2146.0 55 2328.0 56 2420.5 57 2513.0 58 2691.5 59 2870.0 60 2781.0 61 2692.0 62 2554.0 63 2223.0 64 2030.0 65 1762.0 66 1494.0 67 1348.0 68 1202.0 69 1000.5 70 799.0 71 698.5 72 529.5 73 461.0 74 383.0 75 305.0 76 220.5 77 136.0 78 97.5 79 40.0 80 21.0 81 12.5 82 4.0 83 3.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.023746027107003253 14 0.007306469879077924 15 0.2283271837211851 16 0.06393161144193184 17 0.0 18 0.0 19 0.09315749095824352 20 0.09863734336755195 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.014612939758155847 27 0.08950425601870456 28 0.12055675300478574 29 0.02739926204654221 30 0.03470573192562013 31 0.036532349395389616 32 0.08767763854893508 33 0.16074233733971433 34 0.05662514156285391 35 0.04566543674423702 36 0.0383589668651591 37 0.16439557227925328 38 0.27764585540496106 39 0.18996821685602602 40 0.7343002228473313 41 0.9242684397033574 42 0.13151645782340263 43 0.22650056625141565 44 0.5151061264749935 45 0.18814159938625655 46 0.2009279216746429 47 0.029225879516311695 48 0.144302780111789 49 0.5443320059913054 50 0.10959704818616885 51 0.29043217769334745 52 0.11690351806524678 53 0.144302780111789 54 0.2411135060095715 55 0.20275453914441235 56 0.15891571986994482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 54746.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.9690205677127 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42221687905747 65.65411171592444 2 7.946621784082712 12.073941475176268 3 2.5703294061072373 5.857962225550725 4 1.0699687424861746 3.251379096189676 5 0.6011060351045925 2.283271837211851 6 0.3774945900456841 1.720673656522851 7 0.2716999278672758 1.4448544185876595 8 0.17311853811012262 1.052131662587221 9 0.11300793459966339 0.7726591897124905 >10 0.44722289011781674 5.478025791838673 >50 0.00721327242125511 0.4109889306981332 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 81 0.14795601505132797 No Hit AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78 0.1424761626420195 No Hit AACCTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG 66 0.12055675300478574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.010959704818616885 0.0 0.0 0.0 0.0 7 0.012786322288386366 0.0 0.0 0.0 0.0 8 0.012786322288386366 0.0 0.0 0.0 0.0 9 0.014612939758155847 0.0 0.0 0.0 0.0 10 0.03287911445585066 0.0 0.0 0.0 0.0 11 0.04383881927446754 0.0 0.0 0.0 0.0 12 0.047492054214006506 0.0 0.0 0.0 0.0 13 0.051145289153545465 0.0 0.0 0.0 0.0 14 0.05297190662331495 0.0 0.0 0.0 0.0 15 0.05297190662331495 0.0 0.0 0.0 0.0 16 0.056625141562853906 0.0 0.0 0.0 0.0 17 0.06758484638147079 0.0 0.0 0.0 0.0 18 0.07489131626054872 0.0 0.0 0.0 0.0 19 0.08402440360939611 0.0 0.0 0.0 0.0 20 0.09315749095824352 0.0 0.0 0.0 0.0 21 0.1041171957768604 0.0 0.0 0.0 0.0 22 0.11142366565593834 0.0 0.0 0.0 0.0 23 0.12238337047455522 0.0 0.0 0.0 0.0 24 0.1424761626420195 0.0 0.0 0.0 0.0 25 0.15160924999086692 0.0 0.0 0.0 0.0 26 0.17352865962810068 0.0 0.0 0.0 0.0 27 0.1790085120374091 0.0 0.0 0.0 0.0 28 0.18631498191648704 0.0 0.0 0.0 0.0 29 0.19544806926533445 0.0 0.0 0.0 0.0 30 0.19544806926533445 0.0 0.0 0.0 0.0 31 0.20275453914441238 0.0 0.0 0.0 0.0 32 0.20458115661418186 0.0 0.0 0.0 0.0 33 0.20640777408395133 0.0 0.0 0.0 0.0 34 0.2082343915537208 0.0 0.0 0.0 0.0 35 0.21554086143279874 0.0 0.0 0.0 0.0 36 0.2191940963723377 0.0 0.0 0.0 0.0 37 0.2210207138421072 0.0 0.0 0.0 0.0 38 0.2210207138421072 0.0 0.0 0.0 0.0 39 0.2210207138421072 0.0 0.0 0.0 0.0 40 0.2210207138421072 0.0 0.0 0.0 0.0 41 0.22284733131187667 0.0 0.0 0.0 0.0 42 0.22467394878164615 0.0 0.0 0.0 0.0 43 0.2283271837211851 0.0 0.0 0.0 0.0 44 0.23380703613049356 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTGGC 25 2.0018244E-5 50.247005 37 GATTTGC 20 4.0488143E-4 50.247005 37 TCAAGAG 15 0.008256294 50.200737 35 CGTTCGC 15 0.008286424 50.154556 47 GTTCGCG 15 0.008286424 50.154556 48 ACAAAAC 25 2.0238005E-5 50.154552 48 TCGTTCG 15 0.008316636 50.108456 46 GTCTTAA 40 2.57387E-9 50.062447 13 AACCTGC 15 0.008346929 50.062443 42 TCAAAGC 15 0.008346929 50.062443 14 CCCCTGT 15 0.008346929 50.062443 42 GGGCACC 15 0.008377305 50.016518 9 GGTCCGG 15 0.008377305 50.016518 10 CATCTCA 15 0.008377305 50.016518 11 GATGTAT 15 0.008377305 50.016518 9 GGATTCT 15 0.008377305 50.016518 9 GCAGTTA 15 0.008377305 50.016518 12 GCATGGA 15 0.008377305 50.016518 9 GAATCCG 15 0.008377305 50.016518 10 CAAAGCA 15 0.008377305 50.016518 15 >>END_MODULE