FastQCFastQC Report
Fri 17 Jun 2016
SRR1526201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13393
Sequences flagged as poor quality0
Sequence length56
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1591.187187336668409No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG390.2911968938997984No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG330.2463973717613679No Hit
TGTCACGGGGGATACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.23893078473829613No Hit
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG310.23146419771522436No Hit
TGTCACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA150.11199880534607631No Hit
TGTCACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG150.11199880534607631No Hit
TGTCACGGGAAGCATGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT140.10453221832300455No Hit
TGTCACGGGATGTCAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10453221832300455No Hit
TGTCACGGGATGGAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10453221832300455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTTG308.996558E-750.5513336
TTGTCTC309.237956E-750.35984840
TATGCTT309.237956E-750.35984835
CTTGTCT309.237956E-750.35984839
AACGATT150.00813960750.1698147
TCAAAGA309.4848656E-750.1698145
AAGATTA309.4848656E-750.1698148
CTTGTCC150.00813960750.1698149
ACGATTA150.00813960750.1698148
AGATTAA309.4848656E-750.1698149
AAAGATT309.4848656E-750.1698147
CGTCTCA150.00813960750.1698141
ATATGCT354.6517016E-850.16980734
AGTAGCA354.7935828E-849.98120528
GGGTAAG150.00826238749.98120532
GCCAGTA402.3628672E-949.98120525
TGCCAGT402.3628672E-949.98120524
CAAAGAT309.737378E-749.98120546
AAGTGGT150.00826238749.98120510
TCTCAAC150.00826238749.98120543