##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526201_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13393 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.621742701411186 39.0 38.0 39.0 34.0 40.0 2 36.95505114612111 39.0 37.0 39.0 32.0 40.0 3 37.36451877846636 39.0 38.0 39.0 33.0 40.0 4 37.231538863585456 39.0 38.0 39.0 33.0 40.0 5 35.10408422310162 39.0 35.0 39.0 20.0 40.0 6 37.219816321959236 39.0 38.0 39.0 33.0 40.0 7 37.27066377958635 39.0 38.0 39.0 33.0 40.0 8 37.31277533039648 39.0 38.0 39.0 33.0 40.0 9 37.330247144030466 39.0 38.0 39.0 33.0 40.0 10 36.80683939371313 39.0 36.0 39.0 32.0 40.0 11 36.37706264466512 38.0 35.0 39.0 31.0 40.0 12 34.47144030463675 38.0 33.0 39.0 23.0 40.0 13 34.17203016501157 38.0 33.0 39.0 23.0 40.0 14 34.27432240722766 38.0 33.0 39.0 23.0 40.0 15 34.21033375643993 38.0 33.0 39.0 23.0 40.0 16 33.69379526618383 38.0 32.0 39.0 22.0 40.0 17 33.559396699768534 37.0 31.0 39.0 22.0 40.0 18 33.048308818039274 37.0 31.0 39.0 19.0 40.0 19 33.97319495258717 37.0 32.0 39.0 24.0 40.0 20 33.979466885686556 37.0 32.0 39.0 24.0 40.0 21 34.73844545658179 38.0 33.0 39.0 26.0 40.0 22 34.42484880161278 38.0 33.0 39.0 25.0 40.0 23 34.972597625625326 38.0 33.0 39.0 27.0 40.0 24 34.541626222653626 38.0 33.0 39.0 25.0 40.0 25 34.72612558799373 38.0 33.0 39.0 26.0 40.0 26 35.36810274023744 38.0 35.0 39.0 28.0 40.0 27 35.19726722914955 38.0 35.0 39.0 28.0 40.0 28 35.158366310759355 38.0 35.0 39.0 27.0 40.0 29 34.91450757858583 38.0 34.0 39.0 27.0 40.0 30 34.797431494064064 38.0 34.0 39.0 27.0 40.0 31 35.12387067871276 38.0 35.0 39.0 28.0 40.0 32 34.890017173150156 38.0 35.0 39.0 27.0 40.0 33 34.97774957067124 38.0 35.0 39.0 28.0 40.0 34 34.90808631374599 38.0 34.0 39.0 27.0 40.0 35 34.648771746434704 38.0 34.0 39.0 27.0 40.0 36 34.30635406555663 37.0 33.0 39.0 27.0 40.0 37 34.00955723138953 37.0 33.0 38.0 27.0 39.0 38 33.67371014709176 36.0 32.0 38.0 25.0 39.0 39 33.228328231165534 36.0 32.0 38.0 23.0 39.0 40 29.965504367953407 35.0 25.0 38.0 10.0 39.0 41 26.73762413200926 32.0 16.0 38.0 6.0 39.0 42 30.879638617188082 35.0 29.0 38.0 12.0 39.0 43 29.355110878817293 34.0 26.0 38.0 6.0 39.0 44 29.256477264242516 34.0 26.0 38.0 4.0 39.0 45 30.395654446352573 35.0 29.0 38.0 4.0 39.0 46 29.920256850593592 35.0 27.0 38.0 4.0 39.0 47 29.46210707085791 35.0 27.0 38.0 4.0 39.0 48 29.59740162771597 35.0 27.0 38.0 4.0 39.0 49 27.042335548420816 33.0 16.0 38.0 4.0 39.0 50 29.03434630030613 34.0 26.0 38.0 4.0 39.0 51 29.218024341073694 35.0 27.0 38.0 4.0 40.0 52 28.898081087135072 35.0 26.0 38.0 4.0 40.0 53 28.44329127155977 35.0 23.0 38.0 4.0 40.0 54 27.478309564697977 34.0 18.0 38.0 4.0 39.0 55 27.740237437467332 34.0 21.0 38.0 4.0 39.0 56 26.289628910624952 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2406188502115114 1 2 2.1164213534143954 1 3 0.808229697969459 1 4 0.8826602913483583 1 5 4.596642441594064 1 6 1.6731779018195567 1 7 1.737258530835362 1 8 1.3299007164361356 1 9 1.4366398547058594 1 10 1.3248109299899085 1 11 -3.2300261696149946 1 12 -15.462583729873781 1 13 -9.484512961238956 1 14 -6.7783596637853485 1 15 -4.743435420072423 1 16 -22.59298947698327 1 17 -3.992377404121804 1 18 -8.754238822860142 1 19 -6.667267614960139 1 20 -4.302224884929032 1 21 -4.637307294636056 1 22 -0.7830469506127287 1 23 -2.172830633060876 1 24 -1.661344226307456 1 25 -0.7391043409706342 1 26 1.6387980436674283 1 27 0.921503177800588 1 28 1.8533202859518525 1 29 0.35074754987874 1 30 2.0263844012741004 1 31 1.3675960201792563 1 32 0.4103845712108196 1 33 1.4844770374462612 1 34 -1.092396398178117 1 35 0.8791573384762259 1 36 3.0115664364087564 1 37 1.9786020056869518 1 38 1.1838177407817483 1 39 3.94230093403759 1 40 1.3568695681611764 1 41 -9.315317192088663 1 42 -9.08737864463967 1 43 -16.56401999076632 1 44 -22.179132872846104 1 45 -10.634656247175588 1 46 -16.699025547215363 1 47 -23.009469921776414 1 48 -15.395999478570115 1 49 -19.823909987000164 1 50 -14.02739332276486 1 51 -11.29391091114194 1 52 -10.594556703580505 1 53 -10.609531038885716 1 54 -10.013749964847097 1 55 -9.635046605405549 1 56 -5.636545141734768 2 1 1.4721977975799305 2 2 2.3480003007828145 2 3 1.7626156628817355 2 4 1.7984497650325721 2 5 4.5124319152782775 2 6 2.1573884281353415 2 7 1.8109427413616785 2 8 1.9474445760852532 2 9 1.9348854687409442 2 10 1.6055126843758742 2 11 1.0506755847709712 2 12 -3.452057414084308 2 13 -3.488021733168779 2 14 3.1865526169164013 2 15 2.5758628255416127 2 16 1.098238593192164 2 17 2.776043648509777 2 18 -3.4980984719829493 2 19 1.6169429113556504 2 20 1.806547044895531 2 21 2.1977804246621915 2 22 2.360812698510074 2 23 2.4166430511496486 2 24 1.4439189315872838 2 25 2.7942289923626973 2 26 2.06686821910602 2 27 2.230275107625154 2 28 1.0848992333202716 2 29 -0.32995420450722435 2 30 0.629893173203925 2 31 1.9500521605301344 2 32 0.1016126413862537 2 33 0.9335998444638065 2 34 2.353217636909605 2 35 0.1703854086516614 2 36 1.5694611732508577 2 37 1.1329879705992312 2 38 -0.16004190834105714 2 39 -2.864716609822061 2 40 0.037571322547140795 2 41 -12.890755788579893 2 42 -12.810185662183532 2 43 -16.95700244690667 2 44 -17.217729364074174 2 45 -10.894305369982607 2 46 -14.214815020899573 2 47 -19.67262781651326 2 48 -12.185473162780642 2 49 -15.95022577647385 2 50 -11.258972270133281 2 51 -8.107945998861236 2 52 -7.426135650948925 2 53 -7.500759109061157 2 54 -5.290942947303236 2 55 -6.17539748259853 2 56 -3.050580229454063 3 1 1.6053247325644548 3 2 1.5987742945908678 3 3 1.5768571489498484 3 4 2.0120720560542438 3 5 5.020171853358768 3 6 1.5908249606430829 3 7 1.6313761778941895 3 8 1.3220575791812337 3 9 2.0091888743137005 3 10 1.0110854397938311 3 11 1.5150718696006962 3 12 2.5138868583615164 3 13 1.6017615485649657 3 14 3.8490913166068097 3 15 1.3467606583589458 3 16 4.2344615034088875 3 17 3.5840931841134918 3 18 4.359486667335936 3 19 2.6386147379810367 3 20 1.5526770758552786 3 21 2.770535842618841 3 22 2.7385216768382463 3 23 2.6859928963508892 3 24 3.691596950163131 3 25 3.1471701688332843 3 26 3.230954906412528 3 27 2.5646404327025536 3 28 2.394496756540086 3 29 2.9703553930159927 3 30 0.01069812676429649 3 31 1.457792098610625 3 32 2.649600257485325 3 33 2.500163311956065 3 34 1.538976150841492 3 35 2.7771965541625008 3 36 2.882154671702871 3 37 3.888405927255583 3 38 2.4622491133307705 3 39 4.726614659527783 3 40 6.090202901494511 3 41 8.18664359222506 3 42 3.9792880220269957 3 43 0.09872510727289807 3 44 4.0051808526440915 3 45 2.6908339489028457 3 46 5.395092099843463 3 47 -1.1153522747175941 3 48 4.251059344959298 3 49 6.51726648358807 3 50 3.607900794882198 3 51 6.192363598661981 3 52 5.73485506112538 3 53 4.363017980722127 3 54 7.272524544956823 3 55 5.592404374986611 3 56 9.367376426892683 4 1 1.8406188502115128 4 2 2.4006318797301844 4 3 2.552089347092263 4 4 2.6405550281904624 4 5 5.407168757383538 4 6 2.367914743924814 4 7 2.547784846624836 4 8 2.473760365558938 4 9 2.513832837161999 4 10 2.763407421217984 4 11 2.682254532139396 4 12 4.442679428020959 4 13 4.564609845778591 4 14 4.554973669547984 4 15 4.6811259834363455 4 16 4.835080698455322 4 17 5.618148911667667 4 18 3.922954159595996 4 19 4.143258700829335 4 20 4.5433891501586885 4 21 4.1977804246621915 4 22 3.255549540615341 4 23 3.9429588406233336 4 24 4.233392615797804 4 25 3.899492150257437 4 26 1.6984471664744447 4 27 2.230275107625154 4 28 2.1901623912150114 4 29 2.6174142165454057 4 30 3.15620896267761 4 31 2.213210055266977 4 32 1.996349483491521 4 33 2.196757739200649 4 34 1.300586057962235 4 35 1.0651222507569287 4 36 2.5694611732508577 4 37 3.606672181125546 4 38 3.892589670606313 4 39 4.187914969125309 4 40 7.090202901494511 4 41 11.267138948262215 4 42 6.768761706237523 4 43 9.095629132040699 4 44 8.992796951715302 4 45 8.368852524754235 4 46 8.522027084363582 4 47 8.748424815065686 4 48 8.761895258271988 4 49 8.523458434052465 4 50 8.846290887761455 4 51 8.260475053770339 4 52 8.415969612208968 4 53 7.393977733044107 4 54 8.866951789538867 4 55 8.87723409634884 4 56 10.10731450738804 5 1 1.6906188502115143 5 2 2.3664213534143954 5 3 2.191563031302792 5 4 2.2326602913483597 5 5 4.496642441594062 5 6 0.22317790181955388 5 7 1.737258530835362 5 8 1.5632340497694628 5 9 1.5033065213725294 5 10 0.5081442633232456 5 11 1.4533071637183426 5 12 2.1874162701262208 5 13 3.7488203720943787 5 14 2.654973669547985 5 15 3.8732312465942442 5 16 1.6903438563500615 5 17 2.957622595878192 5 18 3.662427843806526 5 19 0.08273238503986136 5 20 2.4144417817376365 5 21 2.829359372030609 5 22 0.2002863827205985 5 23 1.2771693669391198 5 24 3.288655773692547 5 25 2.2442289923627 5 26 1.5721313770007583 5 27 1.9381698444672537 5 28 1.0533202859518553 5 29 2.167414216545403 5 30 1.3930510679407675 5 31 1.884262686845922 5 32 3.143717904544154 5 33 2.617810370779594 5 34 1.6742702684885487 5 35 0.9124906718095573 5 36 1.8115664364087536 5 37 1.0119353390202832 5 38 3.650484407448417 5 39 3.2589676007042527 5 40 5.5402029014945136 5 41 6.301349474578004 5 42 2.7292880220269957 5 43 3.769313342567017 5 44 3.8875337938205625 5 45 2.032010419491076 5 46 0.7509744527846358 5 47 1.0405300782235827 5 48 1.2040005214298866 5 49 4.226090012999833 5 50 2.8226066772351395 5 51 -0.6105775778086056 5 52 -0.09455670358050483 5 53 -1.1928643722190522 5 54 -0.6304166315137643 5 55 -1.1517132720722145 5 56 -2.553211808401432 6 1 0.8406188502115128 6 2 2.716421353414397 6 3 2.2827395018910224 6 4 2.3061897031130627 6 5 4.431936559241123 6 6 2.237883784172496 6 7 1.8666702955412475 6 8 2.1455869909459366 6 9 1.7738947566666425 6 10 2.187556028029128 6 11 1.4562483401889281 6 12 4.631533917185045 6 13 4.484114489741437 6 14 4.496150140136216 6 15 4.052643011300127 6 16 4.881520326938293 6 17 5.348799066466427 6 18 5.594780784982994 6 19 4.932732385039863 6 20 2.6703241346788076 6 21 2.182300548501196 6 22 2.6796981474264854 6 23 1.6859928963508892 6 24 3.985714597221957 6 25 3.2648172276568133 6 26 2.8780137299419337 6 27 2.0352286679966696 6 28 2.688614403598912 6 29 3.0291789224277608 6 30 3.4812863620584125 6 31 1.810733275081219 6 32 3.002541433955919 6 33 3.264869194309007 6 34 2.538976150841492 6 35 2.0124906718095588 6 36 3.293919377585226 6 37 4.006052986079112 6 38 3.6387197015660604 6 39 3.020732306586609 6 40 8.20784996031804 6 41 8.068996533401531 6 42 7.038111551438764 6 43 7.03990157786113 6 44 8.299298499702918 6 45 7.39671630184402 6 46 7.630386217490521 6 47 6.76700066645888 6 48 7.839294639076943 6 49 11.16432530711748 6 50 7.725547853705727 6 51 8.13354006925022 6 52 7.499560943478322 6 53 6.774782686604478 6 54 5.6842892508391785 6 55 5.004169080868962 6 56 5.485023485716216 7 1 1.6641482619762158 7 2 1.3634801769438099 7 3 2.4003865607145585 7 4 2.3061897031130627 7 5 4.667230676888181 7 6 1.9437661371136699 7 7 1.3960820602471316 7 8 1.8514693438871106 7 9 1.1268359331372295 7 10 2.364026616264418 7 11 2.22095422254187 7 12 4.984475093655632 7 13 3.4841144897414367 7 14 4.672620728371513 7 15 3.758525364241301 7 16 5.293285032820648 7 17 5.113504948819369 7 18 4.183016079100639 7 19 4.344497090922218 7 20 3.7879711935023366 7 21 3.182300548501196 7 22 2.0326393238970724 7 23 4.274228190468534 7 24 4.80924400898666 7 25 3.1471701688332843 7 26 0.6427196122948757 7 27 2.3881698444672566 7 28 2.570967344775383 7 29 2.9115318636042318 7 30 3.6577569502937095 7 31 1.575439157434154 7 32 1.649600257485325 7 33 1.08839860607371 7 34 1.538976150841492 7 35 2.071314201221327 7 36 2.588037024644052 7 37 1.947229456667344 7 38 0.2857785250954734 7 39 2.726614659527783 7 40 6.854908783847453 7 41 -2.1662975842455268 7 42 3.9792880220269957 7 43 6.098725107272898 7 44 4.181651440879389 7 45 4.632010419491078 7 46 6.042150923372869 7 47 6.355235960576525 7 48 5.662824050841653 7 49 7.929031189470422 7 50 5.843194912529256 7 51 5.13354006925022 7 52 4.970149178772438 7 53 7.245370921898594 7 54 5.4489951331921205 7 55 8.59240437498661 7 56 4.661494073951509 8 1 1.8406188502115128 8 2 2.583088020081064 8 3 2.3415630313027904 8 4 2.349326958015027 8 5 4.996642441594062 8 6 2.0065112351528853 8 7 2.1372585308353607 8 8 1.92990071643613 8 9 2.1033065213725237 8 10 2.5248109299899113 8 11 1.8366404970516754 8 12 3.2040829367928865 8 13 4.515487038761044 8 14 2.421640336214651 8 15 5.256564579927577 8 16 4.9403438563500615 8 17 6.007622595878196 8 18 5.57909451047319 8 19 4.066065718373196 8 20 5.1644417817376365 8 21 3.5626927053639434 8 22 2.416953049387267 8 23 4.02716936693912 8 24 3.405322440359214 8 25 1.2608956590293658 8 26 1.3054647103340926 8 27 2.2548365111339237 8 28 1.1199869526185182 8 29 1.4840808832120729 8 30 3.226384401274103 8 31 0.8342626868459249 8 32 1.3437179045441496 8 33 2.4178103707795913 8 34 0.24093693515521863 8 35 3.0124906718095588 8 36 0.6782331030754207 8 37 -0.7547313276463825 8 38 -0.14951559255158742 8 39 -0.4576990659624123 8 40 0.6902029014945121 8 41 0.9513494745780022 8 42 -1.4873786446396693 8 43 -0.2973533240996531 8 44 1.4208671271538975 8 45 -0.5679895805089217 8 46 -0.4990255472153642 8 47 1.6571967448902498 8 48 1.4040005214298859 8 49 3.642756679666501 8 50 1.9059400105684752 8 51 1.9727557555247266 8 52 -0.39455670358050554 8 53 0.45713562778094996 8 54 1.85291670181957 8 55 0.6316200612611169 8 56 1.6301215249319014 9 1 1.3112070855056288 9 2 -0.10710805835030612 9 3 1.7533277371851455 9 4 1.8944249972307148 9 5 3.431936559241123 9 6 0.8261190782901409 9 7 0.9254938249530085 9 8 1.4397046380047627 9 9 1.1856594625489976 9 10 1.7757913221467732 9 11 1.4562483401889281 9 12 3.2197692113026903 9 13 2.3076439015061467 9 14 2.0255619048420996 9 15 3.405584187770714 9 16 3.9991673857618295 9 17 4.289975537054666 9 18 4.594780784982994 9 19 4.226850032098689 9 20 4.376206487619989 9 21 3.064653489677667 9 22 1.9738157944853043 9 23 1.8624634845861792 9 24 2.985714597221957 9 25 2.5589348747156393 9 26 1.7015431417066438 9 27 0.09405219740843052 9 28 2.394496756540086 9 29 1.7938848047807028 9 30 2.0695216561760645 9 31 -0.07161966609525194 9 32 2.179012022191209 9 33 2.323692723720768 9 34 -0.284553260923218 9 35 1.7771965541625008 9 36 0.646860554055813 9 37 -0.2292411315679459 9 38 0.5210726427425314 9 39 1.550144071292486 9 40 4.149026430906279 9 41 2.7748788863427087 9 42 2.5675233161446407 9 43 5.216372166096427 9 44 4.5345926173499755 9 45 1.6320104194910776 9 46 3.806856805725811 9 47 5.708177137047112 9 48 5.19223581554753 9 49 6.223148836529248 9 50 2.725547853705727 9 51 2.0158930104266872 9 52 1.3230903552430249 9 53 4.715959157192717 9 54 4.0372304273097654 9 55 1.8865220220454333 9 56 4.367376426892683 10 1 1.9120474216400822 10 2 2.1449927819858274 10 3 2.41299160273136 10 4 2.196946005634075 10 5 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3.115073657744894 115 22 3.078857811292032 115 23 3.1985979383676906 115 24 3.98151291654969 115 25 3.3656575637912667 115 26 2.529274234143614 115 27 2.816741273038687 115 28 2.8818917145232845 115 29 2.688842787973975 115 30 3.107336782226483 115 31 2.9199769725602067 115 32 0.8008607616870052 115 33 2.3320960850653094 115 34 0.917127411345696 115 35 1.0839192432381282 115 36 0.8401378649801856 115 37 1.2833639104488554 115 38 1.3361986931627001 115 39 2.3232533149899695 115 40 5.304488615780226 115 41 6.6656351888637175 115 42 0.9078594505984228 115 43 -5.216400943147271 115 44 -11.398180491893722 115 45 3.203438990919647 115 46 -4.25616840435822 115 47 -1.4094699217764166 115 48 -1.1102851928558302 115 49 -15.281052844143023 115 50 -1.9845361799077175 115 51 -0.8748632920943216 115 52 -0.5802709892947888 115 53 -3.6285786579333354 115 54 -3.294702345799479 115 55 -3.273141843500788 115 56 0.8967881915985672 116 1 1.1072855168781786 116 2 1.9830880200810626 116 3 1.741563031302789 116 4 1.349326958015027 116 5 4.329975774927398 116 6 1.1398445684862182 116 7 0.8705918641686949 116 8 0.7299007164361342 116 9 1.3699731880391965 116 10 1.791477596656577 116 11 -2.630026169614993 116 12 -15.329250396540449 116 13 -10.284512961238956 116 14 -3.1783596637853506 116 15 -10.543435420072424 116 16 -5.926322810316606 116 17 -4.12571073745514 116 18 -10.154238822860144 116 19 -4.133934281626804 116 20 -5.768891551595701 116 21 1.6960260386972763 116 22 0.5502863827205999 116 23 0.2271693669391226 116 24 0.07198910702587824 116 25 1.2608956590293658 116 26 0.9721313770007569 116 27 1.7881698444672551 116 28 0.9866536192851854 116 29 2.5507475498787358 116 30 1.426384401274099 116 31 1.3009293535125863 116 32 1.6770512378774853 116 33 -0.4488562958870759 116 34 1.84093693515522 116 35 2.61249067180956 116 36 1.9448997697420864 116 37 2.9786020056869518 116 38 -0.2828489258849203 116 39 2.0089676007042527 116 40 5.356869568161176 116 41 2.6846828079113365 116 42 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1.0386557736925468 117 25 -1.3057710076373041 117 26 1.8721313770007626 117 27 0.28816984446725513 117 28 -1.746679714048149 117 29 1.417414216545403 117 30 1.3930510679407675 117 31 1.8342626868459249 117 32 -0.056282095455848946 117 33 -0.5821896292204087 117 34 -0.7257297315114499 117 35 -0.2875093281904384 117 36 0.01156643640875643 117 37 -0.18806466097971253 117 38 -3.149515592551584 117 39 1.2089676007042556 117 40 4.690202901494512 117 41 1.1513494745780015 117 42 -3.5207119779730043 117 43 -10.230686657432987 117 44 -10.512466206179436 117 45 -5.367989580508922 117 46 -5.899025547215363 117 47 -5.109469921776416 117 48 -10.795999478570113 117 49 -13.523909987000165 117 50 -10.027393322764858 117 51 -10.760577577808608 117 52 -10.094556703580505 117 53 -9.24286437221905 117 54 -13.680416631513763 117 55 -10.901713272072215 117 56 -9.403211808401434 118 1 0.7295077391003986 118 2 1.271976908969954 118 3 1.2304519201916762 118 4 1.0382158469039169 118 5 4.085531330482951 118 6 1.3620667907084396 118 7 1.2261474197242492 118 8 0.9299007164361299 118 9 0.9699731880391909 118 10 1.54703315221213 118 11 -1.3411372807261017 118 12 -6.1070281743182235 118 13 -8.41784629457229 118 14 -4.889470774896459 118 15 -3.9212131978502 118 16 -11.837433921427717 118 17 -9.036821848566248 118 18 -9.73201660063792 118 19 -3.733934281626805 118 20 -4.146669329373477 118 21 0.9404704831417234 118 22 1.039175271609487 118 23 0.9605027002724569 118 24 1.0053224403592083 118 25 -0.20577100763730272 118 26 1.6165758214452026 118 27 1.4992809555783708 118 28 1.5644313970629682 118 29 1.72852532765652 118 30 1.670828845718546 118 31 1.8564849090681435 118 32 -1.8340598732336275 118 33 1.1733659263351512 118 34 1.9964924907107715 118 35 -0.9875093281904412 118 36 0.8560108808531979 118 37 1.9341575612425075 118 38 3.0504844074484154 118 39 3.608967600704254 118 40 5.090202901494511 118 41 7.840238363466888 118 42 -1.4651564224174471 118 43 -3.097353324099654 118 44 -12.223577317290548 118 45 -2.590211802731144 118 46 -9.010136658326473 118 47 -8.631692143998638 118 48 -11.618221700792336 118 49 -11.423909987000165 118 50 -6.294059989431528 118 51 -5.382799800030828 118 52 -4.683445592469393 118 53 -6.120642149996829 118 54 -7.191527742624874 118 55 -3.368379938738883 118 56 -0.9921006972903221 119 1 0.17395218354484854 119 2 1.0497546867477325 119 3 0.6748963646361261 119 4 0.8159936246816955 119 5 3.5299757749274008 119 6 0.8065112351528896 119 7 0.6705918641686992 119 8 0.5965673831028013 119 9 0.6366398547058623 119 10 0.9081442633232442 119 11 -3.813359502948323 119 12 -6.245917063207113 119 13 -13.91784629457229 119 14 -10.528359663785348 119 15 -11.643435420072425 119 16 -12.14298947698327 119 17 -16.175710737455137 119 18 -14.204238822860145 119 19 -6.067267614960137 119 20 -7.452224884929034 119 21 0.16269270536394487 119 22 1.5669530493872657 119 23 -1.1228306330608788 119 24 1.7553224403592083 119 25 0.2942289923626973 119 26 1.088798043667424 119 27 0.5548365111339209 119 28 1.6199869526185182 119 29 2.1174142165454057 119 30 1.5597177346074318 119 31 2.2175960201792577 119 32 -1.556282095455849 119 33 2.367810370779594 119 34 0.5242702684885501 119 35 2.679157338476223 119 36 2.411566436408755 119 37 1.6286020056869503 119 38 3.7171510741150797 119 39 3.2756342673709185 119 40 5.923536234827846 119 41 2.86801614124467 119 42 1.3959546886936636 119 43 -13.597353324099652 119 44 -14.195799539512771 119 45 -10.701322913842255 119 46 -15.899025547215363 119 47 -19.826136588443084 119 48 -12.479332811903449 119 49 -13.5072433203335 119 50 -15.37739332276486 119 51 -10.077244244475274 119 52 -10.711223370247172 119 53 -10.84286437221905 119 54 -12.413749964847097 119 55 -8.868379938738883 119 56 -9.603211808401433 120 1 -0.49271448312182287 120 2 -0.6169119799189389 120 3 -0.7695480798083238 120 4 0.14932695801502405 120 5 1.9744202193718436 120 6 -1.0823776537360033 120 7 -0.8849636913868579 120 8 0.48545627199168706 120 9 -0.2522490341830306 120 10 -0.008522403343420137 120 11 -2.896692836281659 120 12 -13.66258372987378 120 13 -2.3067351834611785 120 14 -7.889470774896459 120 15 -6.254546531183536 120 16 -13.726322810316606 120 17 -10.814599626344027 120 18 -13.509794378415698 120 19 -11.178378726071248 120 20 -13.035558218262366 120 21 -9.503973961302723 120 22 -7.849713617279399 120 23 -7.150608410838657 120 24 -9.661344226307456 120 25 -3.9835487854150813 120 26 -3.1612019563325724 120 27 -5.167385711088301 120 28 -3.3244574918259246 120 29 -2.27147467234348 120 30 -2.995837820948122 120 31 -2.0324039798207423 120 32 -7.945170984344738 120 33 -2.1599674069981845 120 34 -2.1146186204003357 120 35 -2.76528710596822 120 36 -1.6995446747023557 120 37 -1.5102868832019354 120 38 -0.7272933703293631 120 39 -0.724365732629078 120 40 3.0902029014945107 120 41 -1.937539414310887 120 42 -4.576267533528561 120 43 -12.76401999076632 120 44 -11.779132872846105 120 45 -6.7013229138422545 120 46 -16.56569221388203 120 47 -15.631692143998638 120 48 -13.395999478570115 120 49 -16.868354431444608 120 50 -12.73850443387597 120 51 -9.938355355586385 120 52 -9.239001148024947 120 53 -11.12064214999683 120 54 -13.969305520402653 120 55 -8.479491049849994 120 56 -4.214322919512544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 60.0 5 1.0 6 6.0 7 16.0 8 15.0 9 20.0 10 11.0 11 8.0 12 10.0 13 17.0 14 23.0 15 25.0 16 30.0 17 50.0 18 60.0 19 70.0 20 112.0 21 143.0 22 130.0 23 202.0 24 239.0 25 261.0 26 298.0 27 365.0 28 388.0 29 412.0 30 530.0 31 638.0 32 781.0 33 830.0 34 965.0 35 1171.0 36 1436.0 37 1727.0 38 1533.0 39 810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.32800716792354 0.515194504591951 0.044799522138430525 0.11199880534607631 8 99.5744045396849 0.2538639587844396 0.03733293511535877 0.13439856641529158 9 98.02882102590905 1.2842529679683417 0.42559546031509 0.26133054580751136 10 46.50190397969089 34.57029791682222 10.520421115508102 8.407376987978795 11 31.359665496901368 34.01777047711491 17.673411483610842 16.94915254237288 12 34.734562831329804 14.17158216979019 22.04136489210782 29.052490106772193 13 23.325117633878556 15.467921428037942 27.253715736798867 33.95324520128464 14 22.804659498207887 22.70011947431302 29.853643966547196 24.641577060931898 15 28.412068578273562 18.387362431683762 28.165007112375534 25.03556187766714 16 31.615120274914087 19.37098461078739 24.876736889287315 24.137158225011206 17 27.954901814380644 21.093108340177704 25.408795639513176 25.543194205928472 18 22.914955573807212 24.13200925856791 24.36347345628313 28.589561711341744 19 29.252615844544096 22.623318385650222 21.78624813153961 26.33781763826607 20 26.920203227734607 25.134488942020322 21.301554094441126 26.643753735803944 21 27.775703725826922 21.436571343239006 24.893601134921227 25.894123796012842 22 28.081833793772866 21.9666990218771 22.018965131038602 27.93250205331143 23 26.797580825804523 23.071753901291718 23.221085641753156 26.9095796311506 24 24.916000895990443 23.88561188680654 27.058911371612037 24.13947584559098 25 28.201299186142016 24.39333980437542 24.968267005151944 22.43709400433062 26 23.385350556260732 22.95228850892257 26.364518778466362 27.29784215635033 27 23.118319754839675 19.149413259585916 29.1277374990657 28.604529486508707 28 23.269029460146555 23.665320771646478 29.235830716315235 23.82981905189173 29 25.644089313718172 21.14853259652005 31.909491449480992 21.297886640280787 30 22.087813620071685 19.422043010752688 36.45459976105138 22.035543608124254 31 21.08132327682772 21.67873945187066 35.493988499738634 21.74594877156299 32 24.316041261773062 19.883390641351472 35.82000298998356 19.980565106891913 33 23.137900089739755 18.463954531857613 37.09991026024529 21.298235118157343 34 19.071087216248507 22.356630824372758 39.26971326164875 19.30256869772999 35 19.070740270411594 20.714125644281765 43.7962202136401 16.418913871666543 36 20.92293906810036 23.581242532855438 38.216845878136205 17.278972520908003 37 20.05533537725267 19.56180363418829 42.18948627832199 18.193374710237045 38 22.391857506361323 20.363718006286486 40.6376290974405 16.606795389911692 39 20.34835912386933 19.13732525977424 38.82783882783883 21.686476788517606 40 15.527996993611422 15.197294250281848 38.30890642615558 30.96580232995115 41 14.522321764661598 14.093201836934426 35.02973725814951 36.35473914025446 42 21.063257065948857 19.702407656647225 40.92268580828473 18.31164946911919 43 15.899393667190658 16.62549592035332 42.8026049854031 24.672505427052922 44 17.34954224823653 17.927360048026415 38.713792585922256 26.009305117814797 45 18.70557426112982 20.718294051627385 42.79087167976057 17.78526000748223 46 18.978156792339917 20.788450029922203 39.91621783363256 20.317175344105326 47 16.824733029646776 22.851168695392428 40.908072586065266 19.416025688895527 48 19.054741250373915 23.26503140891415 39.0068800478612 18.673347292850735 49 14.440108075652958 19.701290903632543 36.280396277394175 29.578204743320324 50 20.651767695642427 21.743030121832724 40.645788175498915 16.959414007025934 51 19.842814371257486 23.39071856287425 39.72305389221557 17.043413173652695 52 18.936901913875598 20.947966507177032 41.44736842105263 18.667763157894736 53 17.973831775700937 21.921495327102804 41.74205607476635 18.362616822429906 54 17.372754491017965 24.528443113772454 35.61377245508982 22.48502994011976 55 18.621360676596062 24.578998577950752 38.82942893495996 17.97021181049323 56 21.59958102648511 22.849019901241956 37.655244650606015 17.896154421666914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 7.0 13 23.5 14 33.0 15 37.0 16 41.0 17 51.0 18 61.0 19 75.0 20 89.0 21 125.5 22 210.5 23 259.0 24 319.0 25 379.0 26 421.0 27 463.0 28 494.0 29 591.5 30 658.0 31 621.0 32 584.0 33 503.5 34 423.0 35 358.5 36 294.0 37 252.5 38 199.0 39 187.0 40 204.0 41 221.0 42 230.0 43 239.0 44 256.5 45 274.0 46 385.0 47 485.0 48 474.0 49 466.5 50 459.0 51 438.5 52 418.0 53 486.5 54 537.0 55 519.0 56 565.0 57 611.0 58 622.0 59 633.0 60 655.5 61 678.0 62 674.0 63 670.5 64 671.0 65 619.0 66 567.0 67 499.5 68 432.0 69 404.5 70 377.0 71 320.5 72 236.0 73 208.0 74 160.5 75 113.0 76 87.5 77 62.0 78 45.0 79 27.0 80 26.0 81 17.0 82 8.0 83 6.5 84 5.0 85 3.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.029866348092287016 14 0.007466587023071754 15 0.26879713283058315 16 0.052266109161502275 17 0.0 18 0.0 19 0.0970656312999328 20 0.06719928320764579 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.10453221832300455 28 0.1418651534383633 29 0.014933174046143508 30 0.007466587023071754 31 0.014933174046143508 32 0.11199880534607631 33 0.15679832748450684 34 0.007466587023071754 35 0.044799522138430525 36 0.007466587023071754 37 0.14933174046143508 38 0.23146419771522436 39 0.11946539236914806 40 0.6570596580303144 41 0.8213245725378929 42 0.1418651534383633 43 0.2538639587844396 44 0.5002613305458075 45 0.2090644366460091 46 0.18666467557679384 47 0.014933174046143508 48 0.15679832748450684 49 0.515194504591951 50 0.10453221832300455 51 0.2463973717613679 52 0.1269319793922198 53 0.13439856641529158 54 0.2463973717613679 55 0.23893078473829613 56 0.20159784962293734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 13393.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.07115657432988 #Duplication Level Percentage of deduplicated Percentage of total 1 87.73371104815864 69.37206003135967 2 7.3182247403210585 11.573209885761218 3 2.5118035882908405 5.958336444411259 4 0.9631728045325779 3.0463675054132757 5 0.5476864966949953 2.1653102366908086 6 0.26440037771482533 1.2543866198760547 7 0.1794145420207743 0.9930560740685432 8 0.12275731822474031 0.7765250503994624 9 0.09442870632672333 0.6719928320764578 >10 0.254957507082153 3.001567983274845 >50 0.0 0.0 >100 0.009442870632672332 1.187187336668409 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 159 1.187187336668409 No Hit TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 39 0.2911968938997984 No Hit TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 33 0.2463973717613679 No Hit TGTCACGGGGGATACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.23893078473829613 No Hit TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 31 0.23146419771522436 No Hit TGTCACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 15 0.11199880534607631 No Hit TGTCACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 15 0.11199880534607631 No Hit TGTCACGGGAAGCATGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 14 0.10453221832300455 No Hit TGTCACGGGATGTCAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.10453221832300455 No Hit TGTCACGGGATGGAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.10453221832300455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.007466587023071754 0.0 0.0 0.0 0.0 15 0.007466587023071754 0.0 0.0 0.0 0.0 16 0.022399761069215263 0.0 0.0 0.0 0.0 17 0.022399761069215263 0.0 0.0 0.0 0.0 18 0.022399761069215263 0.0 0.0 0.0 0.0 19 0.029866348092287016 0.0 0.0 0.0 0.0 20 0.029866348092287016 0.0 0.0 0.0 0.0 21 0.044799522138430525 0.0 0.0 0.0 0.0 22 0.05973269618457403 0.0 0.0 0.0 0.0 23 0.05973269618457403 0.0 0.0 0.0 0.0 24 0.05973269618457403 0.0 0.0 0.0 0.0 25 0.08213245725378929 0.0 0.0 0.0 0.0 26 0.08213245725378929 0.0 0.0 0.0 0.0 27 0.08959904427686105 0.0 0.0 0.0 0.0 28 0.11199880534607631 0.0 0.0 0.0 0.0 29 0.11199880534607631 0.0 0.0 0.0 0.0 30 0.11199880534607631 0.0 0.0 0.0 0.0 31 0.1269319793922198 0.0 0.0 0.0 0.0 32 0.1269319793922198 0.0 0.0 0.0 0.0 33 0.1269319793922198 0.0 0.0 0.0 0.0 34 0.1269319793922198 0.0 0.0 0.0 0.0 35 0.1269319793922198 0.0 0.0 0.0 0.0 36 0.13439856641529158 0.0 0.0 0.0 0.0 37 0.1418651534383633 0.0 0.0 0.0 0.0 38 0.1418651534383633 0.0 0.0 0.0 0.0 39 0.14933174046143508 0.0 0.0 0.0 0.0 40 0.14933174046143508 0.0 0.0 0.0 0.0 41 0.14933174046143508 0.0 0.0 0.0 0.0 42 0.16426491450757857 0.0 0.0 0.0 0.0 43 0.16426491450757857 0.0 0.0 0.0 0.0 44 0.16426491450757857 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGCTTG 30 8.996558E-7 50.55133 36 TTGTCTC 30 9.237956E-7 50.359848 40 TATGCTT 30 9.237956E-7 50.359848 35 CTTGTCT 30 9.237956E-7 50.359848 39 AACGATT 15 0.008139607 50.16981 47 TCAAAGA 30 9.4848656E-7 50.16981 45 AAGATTA 30 9.4848656E-7 50.16981 48 CTTGTCC 15 0.008139607 50.16981 49 ACGATTA 15 0.008139607 50.16981 48 AGATTAA 30 9.4848656E-7 50.16981 49 AAAGATT 30 9.4848656E-7 50.16981 47 CGTCTCA 15 0.008139607 50.16981 41 ATATGCT 35 4.6517016E-8 50.169807 34 AGTAGCA 35 4.7935828E-8 49.981205 28 GGGTAAG 15 0.008262387 49.981205 32 GCCAGTA 40 2.3628672E-9 49.981205 25 TGCCAGT 40 2.3628672E-9 49.981205 24 CAAAGAT 30 9.737378E-7 49.981205 46 AAGTGGT 15 0.008262387 49.981205 10 TCTCAAC 15 0.008262387 49.981205 43 >>END_MODULE