FastQCFastQC Report
Fri 17 Jun 2016
SRR1526200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93467
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4690.5017813773845314No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2900.31026993484331367No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2580.2760332523778446No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1930.20648999111986047No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1380.14764569313233547No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1340.14336610782415185No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1090.11661869964800411No Hit
CCATCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1050.11233911433982047No Hit
CCATCTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA980.1048498400504991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA354.7972208E-850.53695740
CTACGCT150.00812851850.4273337
CAGCGTA150.00818095650.34542536
ACCGACA150.00818095650.34542536
CCTACGC150.00818095650.34542536
ATGCCTG150.00818095650.34542536
CGACTCC150.00821605350.29096644
GTAAGAC355.0282324E-850.23663334
TAGGATC150.00825126450.23662634
CAACGAT451.2914825E-1050.1824146
AACGATT451.2914825E-1050.1824147
CGGCACG150.00828658550.18240749
ACGATTA252.0283442E-550.18240748
TCCAAAG150.00839322650.0204433
AGATGTG204.1998795E-449.91304813
TATCCGC150.00846488449.9130413
GAATGGT252.1007076E-549.886279
TAGAGAT204.2110248E-449.88626510
GAAGCTT204.2110248E-449.8862659
GATGTAT150.0084828749.8862659
Produced by FastQC (version 0.11.5)