FastQCFastQC Report
Fri 17 Jun 2016
SRR1526199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21944
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2811.2805322639445862No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG740.3372220196864747No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG610.27798031352533725No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG530.24152387896463728No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG430.19595333576376228No Hit
CGTGGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC310.14126868392271236No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC290.13215457528253735No Hit
CGTGGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.12304046664236237No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC230.1048122493620124No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.1048122493620124No Hit
CGTGGCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAACAAAAAAAAAAAAAAA220.10025519504192489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA203.88183E-450.53828441
TGCCTAG150.00797509450.5382843
TCTCAAC251.895472E-550.5382843
ACAAAAC150.00804874250.42129548
AACGATT251.9217912E-550.42129547
ACGATTA150.00804874250.42129548
AGCAAGC150.00804874250.42129548
CCTAGCA150.00804874250.42129545
TTGCCTA150.00804874250.42129542
CGATTAA150.00804874250.42129549
GATTCAG204.0637256E-450.07356350
ATATGCT750.049.95871734
TGGTTGA950.049.95871414
GGTTGAT950.049.95871415
TATGCTT650.049.95871435
TTGGGGA150.00834840649.95871414
ATGCTTG650.049.95871436
ATATGCC150.00834840649.95871434
ATCCTGC950.049.84439520
GTTGATC950.049.84439516