FastQCFastQC Report
Fri 17 Jun 2016
SRR1526198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6947
Sequences flagged as poor quality0
Sequence length56
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.575788109975529No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC290.4174463797322585No Hit
ATCTCTGGGAGTCAACAATCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.30228875773715275No Hit
ATCTCTGGGAAGTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT140.20152583849143516No Hit
ATCTCTGGGTGGCATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.20152583849143516No Hit
ATCTCTGGGGGCTTTTTCGCTCTCTTGCTTCCCGCTCTCTCTTGCTTTTCTCTCTC140.20152583849143516No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTACCAAGCGC120.1727364329926587No Hit
ATCTCTGGGATGTAACGAGAGCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1727364329926587No Hit
ATCTCTGGGAAGCAGTGTAGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT110.15834173024327047No Hit
ATCTCTGGGAAGTGGTATCAACGCAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.15834173024327047No Hit
ATCTCTGGGGACCGCAGAGCCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.14394702749388225No Hit
ATCTCTGGGGGTGCTATCTCTGGGAAGTTCAACGTCAGATCGACTTTTTTTTTTTT100.14394702749388225No Hit
ATCTCTGGGGTATCCTCATCCCAAACTCTCTCTGAGCTGATACCCGAAGAGCCAGG100.14394702749388225No Hit
ATCTCTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.14394702749388225No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC90.12955232474449402No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.12955232474449402No Hit
ATCTCTGGGAAGCATGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT90.12955232474449402No Hit
ATCTCTGGGTGCCGCAGAGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12955232474449402No Hit
ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTT90.12955232474449402No Hit
ATCTCTGGGATGTCACCAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12955232474449402No Hit
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG80.1151576219951058No Hit
ATCTCTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC80.1151576219951058No Hit
ATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG80.1151576219951058No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA80.1151576219951058No Hit
ATCTCTGGGATCCGCAGAGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1151576219951058No Hit
ATCTCTGGGATGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGAAGCATGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA70.10076291924571758No Hit
ATCTCTGGGAACATGGTATAACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG70.10076291924571758No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC70.10076291924571758No Hit
ATCTCTGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA70.10076291924571758No Hit
ATCTCTGGGCATGTAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGTTACGCAGACGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGAGTGTTACGCAGAGTCGACTTTTTTTTTTTTCTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGTACCGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGAACGCAGAGTCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit
ATCTCTGGGATCGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGT70.10076291924571758No Hit
ATCTCTGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10076291924571758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGAG451.0186341E-1049.8695686
TGGGAGT451.0186341E-1049.8695686
GGATGTC150.00816636349.8695648
CAGCAAC150.00816636349.86956421
CTGGGTG251.9070752E-549.8695645
CTGGGGG600.049.8695645
CTGGGGA600.049.8695645
CTGGGCT203.949884E-449.8695645
GAAGCAT251.9070752E-549.8695649
GGGAGTG203.949884E-449.8695647
ATCAGCA150.00816636349.86956419
TCAGCAA150.00816636349.86956420
TGGGGGG251.9070752E-549.8695646
TGGGGGC150.00816636349.8695646
TGGGGCT150.00816636349.8695646
TCGTCTT150.00816636349.86956411
GTCTTAA150.00816636349.86956413
TAATCAG150.00816636349.86956417
GGGATGT150.00816636349.8695647
GGGATGG203.949884E-449.8695647
Produced by FastQC (version 0.11.5)