##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526198_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6947 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.73672088671369 39.0 38.0 40.0 35.0 40.0 2 37.045775154743055 39.0 38.0 40.0 32.0 40.0 3 36.718871455304445 39.0 38.0 40.0 31.0 40.0 4 36.967467971786384 39.0 38.0 40.0 32.0 40.0 5 36.73801640996113 39.0 38.0 40.0 32.0 40.0 6 36.92082913487837 39.0 38.0 40.0 32.0 40.0 7 37.147689650208726 39.0 38.0 40.0 33.0 40.0 8 37.128112854469556 39.0 38.0 40.0 33.0 40.0 9 37.13804519936663 39.0 38.0 40.0 33.0 40.0 10 36.73744062185116 39.0 37.0 40.0 31.0 40.0 11 36.101338707355694 38.0 35.0 40.0 30.0 40.0 12 33.95393695120196 38.0 33.0 40.0 19.0 40.0 13 33.643155318842666 38.0 32.0 39.0 18.0 40.0 14 33.95494458039442 38.0 33.0 39.0 20.0 40.0 15 33.89578235209443 38.0 33.0 40.0 19.0 40.0 16 33.209730819058585 38.0 31.0 39.0 19.0 40.0 17 33.262991219231324 38.0 31.0 39.0 20.0 40.0 18 32.57060601698575 37.0 30.0 39.0 17.0 40.0 19 33.61551748956384 38.0 31.0 39.0 22.0 40.0 20 33.471570462069955 37.0 31.0 39.0 22.0 40.0 21 34.518929034115445 38.0 33.0 39.0 24.0 40.0 22 34.37570174175903 38.0 33.0 39.0 23.0 40.0 23 34.6306319274507 38.0 33.0 39.0 25.0 40.0 24 34.34460918382035 38.0 33.0 39.0 23.0 40.0 25 34.44839499064344 38.0 33.0 39.0 24.0 40.0 26 35.35972362170721 38.0 35.0 39.0 28.0 40.0 27 35.20800345472866 38.0 35.0 39.0 27.0 40.0 28 35.2071397725637 38.0 35.0 39.0 27.0 40.0 29 35.14337123938391 38.0 35.0 39.0 27.0 40.0 30 35.101770548438175 38.0 35.0 39.0 27.0 40.0 31 35.430977400316685 38.0 35.0 39.0 29.0 40.0 32 35.1764790557075 38.0 35.0 39.0 28.0 40.0 33 35.393695120195765 38.0 35.0 39.0 29.0 40.0 34 35.31596372534907 38.0 35.0 40.0 29.0 40.0 35 35.217935799625735 38.0 35.0 40.0 29.0 40.0 36 34.240679429969774 38.0 34.0 39.0 27.0 40.0 37 34.022023895206566 37.0 33.0 39.0 26.0 40.0 38 33.758888728947746 37.0 32.0 39.0 25.0 40.0 39 33.583129408377715 36.0 32.0 39.0 24.0 40.0 40 30.065208003454728 35.0 26.0 39.0 8.0 40.0 41 26.43788685763639 32.0 15.0 38.0 5.0 39.0 42 30.918813876493452 35.0 29.0 38.0 10.0 40.0 43 29.28141643875054 34.0 26.0 38.0 4.0 40.0 44 29.24427810565712 34.0 26.0 38.0 4.0 40.0 45 30.532747948754857 35.0 29.0 38.0 4.0 40.0 46 30.392543543975815 35.0 28.0 39.0 4.0 40.0 47 29.861954800633367 35.0 27.0 38.0 4.0 40.0 48 30.05714696991507 35.0 27.0 38.0 4.0 40.0 49 27.134734417734276 34.0 15.0 38.0 4.0 40.0 50 29.698430977400317 35.0 27.0 38.0 4.0 40.0 51 29.88988052396718 35.0 28.0 39.0 4.0 40.0 52 29.501943284871167 35.0 27.0 39.0 4.0 40.0 53 29.152152008061034 35.0 26.0 39.0 4.0 40.0 54 28.1271052252771 35.0 19.0 39.0 4.0 40.0 55 28.528861379012522 35.0 24.0 39.0 4.0 40.0 56 27.8870015834173 35.0 17.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3276551226551163 1 2 1.8962993025493091 1 3 2.662116101491101 1 4 2.2880026455026297 1 5 2.3914219576719518 1 6 2.3380321067821015 1 7 1.9986273448773417 1 8 2.0077889009139014 1 9 2.085151815776811 1 10 2.083676948051945 1 11 -0.0012229437229436257 1 12 -7.978967051467052 1 13 -8.228414201539188 1 14 -0.31245791245791565 1 15 -1.5239685545935586 1 16 -24.363867845117845 1 17 -5.1738642376142465 1 18 -18.650583814333807 1 19 -6.383235930735935 1 20 -8.72650072150072 1 21 -6.757499098124093 1 22 -4.978785774410767 1 23 -4.6237554112554164 1 24 -6.91469456469455 1 25 -5.951000481000484 1 26 2.417521043771039 1 27 3.828640572390576 1 28 3.7668768037518205 1 29 3.564249639249624 1 30 2.7479605579605604 1 31 3.1341194684944824 1 32 1.8295331289081318 1 33 2.45609788359787 1 34 -0.9702011183261234 1 35 -1.1552816859066724 1 36 0.5075532106782035 1 37 0.13638377825877512 1 38 0.8926316738816666 1 39 0.35241071428569626 1 40 5.116360329485339 1 41 -6.830558261183263 1 42 -10.110604858104853 1 43 -11.983683561808565 1 44 -23.14104467291968 1 45 -9.520663780663789 1 46 -15.426395202020206 1 47 -23.835031565656582 1 48 -15.858916546416541 1 49 -21.20647396584896 1 50 -14.240989057239052 1 51 -12.687184042809044 1 52 -11.983159571909571 1 53 -12.06204785954786 1 54 -10.932784090909092 1 55 -11.07801346801347 1 56 -6.039831649831662 2 1 1.7026551226551163 2 2 2.4462993025493063 2 3 3.037116101491101 2 4 2.6630026455026297 2 5 2.2164219576719546 2 6 2.7130321067821015 2 7 1.9736273448773431 2 8 1.5827889009139042 2 9 2.5601518157768126 2 10 2.383676948051942 2 11 1.773777056277055 2 12 -0.17896705146705472 2 13 -2.1284142015391865 2 14 1.8125420875420843 2 15 2.75103144540644 2 16 4.011132154882155 2 17 3.9011357623857563 2 18 -1.6755838143338053 2 19 3.391764069264063 2 20 3.9734992784992826 2 21 3.617500901875907 2 22 4.44621422558923 2 23 4.176244588744581 2 24 2.535305435305453 2 25 4.198999518999514 2 26 3.2675210437710405 2 27 3.878640572390573 2 28 4.491876803751822 2 29 4.464249639249623 2 30 4.37296055796056 2 31 2.2591194684944824 2 32 3.1045331289081304 2 33 3.6810978835978716 2 34 3.4797988816738794 2 35 3.694718314093329 2 36 5.032553210678202 2 37 3.6613837782587737 2 38 5.417631673881665 2 39 3.7024107142856977 2 40 9.616360329485339 2 41 -2.930558261183265 2 42 -5.835604858104855 2 43 -9.783683561808566 2 44 -14.11604467291968 2 45 -4.295663780663787 2 46 -7.651395202020208 2 47 -15.110031565656582 2 48 -7.158916546416542 2 49 -11.30647396584896 2 50 -4.615989057239052 2 51 -2.3121840428090437 2 52 -1.7081595719095723 2 53 -0.6370478595478595 2 54 -3.0577840909090916 2 55 -3.1030134680134687 2 56 -0.3398316498316625 3 1 1.7935642135642098 3 2 2.7372083934584026 3 3 3.1280251924001945 3 4 2.6630026455026297 3 5 3.0164219576719518 3 6 2.803941197691195 3 7 2.464536435786435 3 8 2.0191525372775345 3 9 2.5510609066859047 3 10 1.6745860389610385 3 11 1.1919588744588765 3 12 5.157396584896581 3 13 3.2261312530062654 3 14 5.085269360269358 3 15 2.441940536315535 3 16 4.147495791245788 3 17 4.2465903078403 3 18 5.76987073112074 3 19 3.5372186147186113 3 20 5.0916810966811 3 21 3.8447736291486336 3 22 4.464396043771053 3 23 5.012608225108217 3 24 3.9716690716690835 3 25 3.8444540644540623 3 26 3.803884680134672 3 27 2.6695496632996694 3 28 3.846422258297274 3 29 2.4733405483405306 3 30 4.100233285233287 3 31 2.531846741221756 3 32 3.3863513107263117 3 33 2.08109788359787 3 34 2.0979806998556967 3 35 2.2765364959115075 3 36 3.54164411976911 3 37 4.306838323713322 3 38 3.1994498556998465 3 39 3.1024107142856963 3 40 7.752723965848972 3 41 8.987623556998557 3 42 3.673486050986053 3 43 -1.574592652717655 3 44 -2.10695376382877 3 45 5.831608946608938 3 46 1.8576957070707039 3 47 -2.164577020202035 3 48 1.5047198172198222 3 49 5.020798761423766 3 50 2.974920033670042 3 51 4.14236141173641 3 52 4.846385882635882 3 53 2.835679413179413 3 54 6.351306818181815 3 55 5.569713804713803 3 56 8.051077441077432 4 1 1.8026551226551177 4 2 3.0462993025493077 4 3 3.1371161014911024 4 4 2.8630026455026325 4 5 3.2164219576719546 4 6 2.313032106782103 4 7 2.7736273448773403 4 8 1.1827889009138985 4 9 1.5601518157768126 4 10 1.4836769480519436 4 11 2.773777056277055 4 12 5.1210329485329495 4 13 5.071585798460809 4 14 3.6125420875420815 4 15 3.451031445406443 4 16 4.911132154882154 4 17 6.001135762385751 4 18 6.324416185666195 4 19 5.291764069264062 4 20 2.77349927849928 4 21 3.917500901875904 4 22 4.046214225589232 4 23 3.476244588744585 4 24 4.735305435305449 4 25 3.4989995189995184 4 26 3.5675210437710376 4 27 2.878640572390573 4 28 4.091876803751823 4 29 3.4642496392496227 4 30 2.472960557960562 4 31 4.459119468494485 4 32 4.204533128908132 4 33 4.281097883597873 4 34 3.4797988816738794 4 35 4.094718314093328 4 36 5.532553210678202 4 37 5.661383778258774 4 38 3.7176316738816695 4 39 5.902410714285693 4 40 10.516360329485337 4 41 10.669441738816737 4 42 7.164395141895145 4 43 8.316316438191432 4 44 6.283955327080317 4 45 6.304336219336214 4 46 7.848604797979792 4 47 7.589968434343415 4 48 7.941083453583456 4 49 8.093526034151036 4 50 8.784010942760947 4 51 4.887815957190959 4 52 2.391840428090429 4 53 3.9629521404521384 4 54 6.942215909090908 4 55 4.996986531986533 4 56 7.160168350168341 5 1 1.988369408369401 5 2 2.932013588263594 5 3 3.3228303872053857 5 4 2.9487169312169144 5 5 3.1592791005290977 5 6 2.998746392496386 5 7 2.6593416305916264 5 8 2.668503186628186 5 9 2.745866101491096 5 10 2.8693912337662297 5 11 3.5166341991342023 5 12 3.9496043771043787 5 13 5.771585798460812 5 14 5.669684944684938 5 15 5.065317159692157 5 16 5.939703583453586 5 17 6.272564333814323 5 18 5.795844757094763 5 19 4.563192640692634 5 20 5.987784992784995 5 21 3.974643759018761 5 22 5.360499939874948 5 23 3.5191017316017295 5 24 4.478162578162596 5 25 4.870428090428085 5 26 3.5960924723424696 5 27 3.7214977152477218 5 28 3.3918768037518205 5 29 3.1356782106781935 5 30 4.2301034151034145 5 31 3.4019766113516283 5 32 2.4902474146224165 5 33 1.0810978835978702 5 34 1.1369417388167307 5 35 1.666146885521897 5 36 2.918267496392488 5 37 4.3328123496873445 5 38 2.589060245310236 5 39 3.530982142857127 5 40 9.402074615199624 5 41 10.598013167388167 5 42 8.335823713323716 5 43 8.944887866762866 5 44 8.698241041366035 5 45 7.532907647907642 5 46 6.948604797979794 5 47 7.147111291486272 5 48 3.5696548821548895 5 49 6.007811748436755 5 50 3.5982966570466637 5 51 3.6878159571909563 5 52 3.8204118566618597 5 53 0.42009499759499747 5 54 0.44221590909090835 5 55 1.1541293891293876 5 56 0.9601683501683382 6 1 2.2582106782106734 6 2 3.3129659692159734 6 3 3.481560545935544 6 4 3.329669312169294 6 5 3.683088624338616 6 6 3.2685876623376586 6 7 3.040294011544006 6 8 1.9383444564694585 6 9 3.1268184824434755 6 10 2.9170102813852807 6 11 3.595999278499278 6 12 5.298810726310727 6 13 5.438252465127476 6 14 4.923653198653199 6 15 5.12880922318422 6 16 6.7333543771043765 6 17 5.145580206830196 6 18 5.113305074555079 6 19 5.769541847041843 6 20 4.6068326118326155 6 21 5.339723124098128 6 22 5.20176978114479 6 23 5.598466810966805 6 24 5.8908609908610075 6 25 5.076777296777294 6 26 4.723076599326596 6 27 5.023085016835019 6 28 4.502987914862935 6 29 5.008694083694067 6 30 4.817405002405003 6 31 4.259119468494482 6 32 4.426755351130353 6 33 3.969986772486756 6 34 4.057576659451655 6 35 3.9836072029822134 6 36 6.076997655122646 6 37 5.761383778258775 6 38 5.795409451659445 6 39 2.991299603174582 6 40 10.005249218374225 6 41 4.058330627705626 6 42 4.097728475228482 6 43 5.849649771524771 6 44 4.98395532708032 6 45 3.326558441558433 6 46 2.504160353535351 6 47 4.956635101010082 6 48 4.36330567580568 6 49 6.737970478595482 6 50 4.884010942760948 6 51 5.132260401635399 6 52 1.6140626503126505 6 53 -0.6592700817700816 6 54 2.553327020202019 6 55 3.0747643097643085 6 56 1.404612794612781 7 1 1.9248773448773377 7 2 3.2018548581048663 7 3 3.1482272126022153 7 4 3.218558201058187 7 5 3.3497552910052875 7 6 3.2685876623376586 7 7 2.3736273448773417 7 8 2.9383444564694585 7 9 3.0157073713323683 7 10 2.4725658369408308 7 11 3.151554834054835 7 12 5.409921837421834 7 13 4.882696909571926 7 14 5.590319865319863 7 15 5.684364778739777 7 16 6.288909932659934 7 17 6.145580206830196 7 18 6.224416185666193 7 19 6.436208513708507 7 20 5.051277056277058 7 21 4.117500901875907 7 22 3.4239920033670117 7 23 1.4873556998556978 7 24 3.5575276575276717 7 25 3.96566618566618 7 26 2.723076599326596 7 27 3.4675294612794616 7 28 3.9474323593073777 7 29 3.008694083694067 7 30 3.595182780182782 7 31 0.7035639129389253 7 32 3.760088684463689 7 33 1.969986772486756 7 34 1.8353544372294337 7 35 0.9836072029822134 7 36 3.6325532106782035 7 37 2.9836060004809966 7 38 3.684298340548331 7 39 2.6579662698412534 7 40 8.449693662818675 7 41 4.947219516594515 7 42 5.764395141895147 7 43 6.960760882635878 7 44 6.98395532708032 7 45 4.993225108225097 7 46 6.393049242424237 7 47 4.734412878787861 7 48 4.807750120250123 7 49 9.404637145262154 7 50 5.328455387205391 7 51 6.798927068302071 7 52 4.836284872534872 7 53 5.8962854737854755 7 54 5.775549242424244 7 55 3.29698653198653 7 56 1.2935016835016704 8 1 0.07765512265511632 8 2 3.146299302549309 8 3 2.662116101491101 8 4 3.1630026455026297 8 5 3.3914219576719518 8 6 2.8380321067821015 8 7 2.8736273448773417 8 8 2.8827889009139014 8 9 2.960151815776811 8 10 1.583676948051945 8 11 3.7487770562770564 8 12 5.146032948532948 8 13 5.396585798460812 8 14 5.187542087542084 8 15 4.976031445406441 8 16 4.511132154882155 8 17 6.3261357623857535 8 18 6.974416185666193 8 19 5.491764069264065 8 20 6.39849927849928 8 21 5.117500901875907 8 22 4.896214225589233 8 23 4.8762445887445836 8 24 5.08530543530545 8 25 4.923999518999516 8 26 4.042521043771039 8 27 4.078640572390576 8 28 2.7668768037518205 8 29 2.689249639249624 8 30 4.62296055796056 8 31 4.134119468494482 8 32 3.079533128908132 8 33 2.45609788359787 8 34 4.404798881673877 8 35 3.2197183140933276 8 36 4.3825532106782035 8 37 5.511383778258775 8 38 4.392631673881667 8 39 4.602410714285696 8 40 8.741360329485339 8 41 -1.0805582611832634 8 42 6.514395141895147 8 43 8.516316438191435 8 44 8.73395532708032 8 45 6.979336219336211 8 46 7.073604797979794 8 47 8.289968434343418 8 48 7.016083453583459 8 49 8.54352603415104 8 50 6.884010942760948 8 51 6.437815957190956 8 52 7.641840428090429 8 53 8.81295214045214 8 54 9.067215909090908 8 55 8.67198653198653 8 56 6.335168350168338 9 1 2.3693217893217806 9 2 3.2018548581048663 9 3 3.9260049903799867 9 4 3.4407804232804082 9 5 3.683088624338616 9 6 3.49080988455988 9 7 3.2625162337662275 9 8 3.271677789802787 9 9 3.349040704665697 9 10 3.4725658369408308 9 11 3.8182215007214992 9 12 5.965477392977391 9 13 6.327141354016369 9 14 5.256986531986527 9 15 5.12880922318422 9 16 6.622243265993269 9 17 6.923357984607975 9 18 7.002193963443972 9 19 6.658430735930729 9 20 5.051277056277058 9 21 4.673056457431464 9 22 4.979547558922569 9 23 5.598466810966805 9 24 4.8908609908610075 9 25 5.187888407888401 9 26 3.3897432659932605 9 27 3.4675294612794616 9 28 1.8363212481962634 9 29 3.6753607503607384 9 30 3.261849446849446 9 31 2.481341690716704 9 32 3.6489775733525747 9 33 3.6366534391534273 9 34 2.7242433261183194 9 35 3.205829425204442 9 36 4.743664321789318 9 37 5.428050444925439 9 38 5.239853896103888 9 39 4.880188492063475 9 40 8.894138107263117 9 41 6.836108405483401 9 42 6.653284030784032 9 43 6.182983104858099 9 44 7.650621993746984 9 45 6.771002886002876 9 46 4.170827020202015 9 47 4.401079545454532 9 48 4.585527898027902 9 49 5.960192700817704 9 50 5.10623316498317 9 51 5.2433715127465135 9 52 4.502951539201543 9 53 4.56295214045214 9 54 5.9977714646464655 9 55 5.630319865319866 9 56 0.2935016835016704 10 1 1.8137662337662306 10 2 1.6462993025493091 10 3 3.037116101491101 10 4 2.5518915343915154 10 5 3.127533068783066 10 6 2.1574765512265444 10 7 1.8180717893217846 10 8 2.4939000120250157 10 9 2.5712629268879255 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115 50 -5.973131914381909 115 51 -7.883612614237617 115 52 -8.608159571909571 115 53 -8.722762145262145 115 54 -13.41492694805195 115 55 -5.988727753727755 115 56 -6.754117364117377 116 1 0.4526551226551163 116 2 1.7712993025493091 116 3 1.287116101491101 116 4 1.4130026455026297 116 5 2.0164219576719518 116 6 0.7130321067821015 116 7 0.9986273448773417 116 8 1.5077889009139014 116 9 1.5851518157768112 116 10 1.583676948051945 116 11 -2.6262229437229436 116 12 -17.228967051467052 116 13 -17.478414201539188 116 14 -4.687457912457916 116 15 -10.398968554593559 116 16 -2.988867845117845 116 17 -2.2988642376142465 116 18 -7.900583814333807 116 19 -3.1332359307359354 116 20 -3.47650072150072 116 21 3.617500901875907 116 22 3.521214225589233 116 23 3.6262445887445836 116 24 1.7103054353054503 116 25 2.9239995189995156 116 26 2.792521043771039 116 27 1.8286405723905759 116 28 3.2668768037518205 116 29 3.564249639249624 116 30 2.7479605579605604 116 31 3.2591194684944824 116 32 2.329533128908132 116 33 2.08109788359787 116 34 3.0297988816738766 116 35 2.5947183140933276 116 36 4.0075532106782035 116 37 3.511383778258775 116 38 4.392631673881667 116 39 4.227410714285696 116 40 9.116360329485339 116 41 2.0444417388167366 116 42 -0.8606048581048533 116 43 -9.608683561808565 116 44 -9.76604467291968 116 45 -0.2706637806637886 116 46 -1.9263952020202062 116 47 -0.8350315656565819 116 48 -1.2339165464165411 116 49 -9.33147396584896 116 50 -2.2409890572390516 116 51 -4.687184042809044 116 52 -3.358159571909571 116 53 -3.56204785954786 116 54 -15.182784090909092 116 55 -2.32801346801347 116 56 -2.6648316498316618 117 1 -0.2973448773448837 117 2 0.6462993025493091 117 3 1.037116101491101 117 4 0.6630026455026297 117 5 1.0164219576719518 117 6 0.7130321067821015 117 7 0.3736273448773417 117 8 0.3827889009139014 117 9 0.4601518157768112 117 10 0.583676948051945 117 11 -1.4595562770562793 117 12 -10.14563371813372 117 13 -10.06174753487252 117 14 -15.520791245791248 117 15 -10.648968554593559 117 16 -20.488867845117845 117 17 -14.632197570947579 117 18 -17.775583814333807 117 19 -7.174902597402603 117 20 -9.559834054834052 117 21 -3.049165764790761 117 22 -2.853785774410767 117 23 -1.4570887445887521 117 24 -0.831361231361214 117 25 -0.7010004810004844 117 26 0.16752104377103905 117 27 0.7453072390572402 117 28 0.22521013708515625 117 29 0.3975829725829598 117 30 0.7062938912938961 117 31 1.7591194684944824 117 32 -2.128800204425204 117 33 0.5810978835978702 117 34 1.1131322150072123 117 35 0.5947183140933276 117 36 1.1325532106782035 117 37 2.261383778258775 117 38 1.8509650072150023 117 39 2.2690773809523606 117 40 5.783026996152003 117 41 0.16944173881673663 117 42 -4.402271524771521 117 43 -18.317016895141897 117 44 -20.01604467291968 117 45 -3.0623304473304565 117 46 -4.551395202020206 117 47 -7.210031565656582 117 48 -5.192249879749873 117 49 -16.70647396584896 117 50 -5.449322390572384 117 51 4.521149290524292 117 52 3.391840428090429 117 53 4.8962854737854755 117 54 3.108882575757576 117 55 5.29698653198653 117 56 7.1268350168350025 118 1 0.5597979797979704 118 2 1.5034421596921632 118 3 1.894258958633955 118 4 1.5201455026454838 118 5 1.8735648148148059 118 6 1.141603535353532 118 7 0.9450559163059111 118 8 1.2399317580567555 118 9 1.3172946729196653 118 10 1.4408198051947991 118 11 -0.6262229437229436 118 12 -7.193252765752767 118 13 -11.228414201539188 118 14 -7.330315055315058 118 15 -1.9346828403078433 118 16 -7.488867845117845 118 17 -11.584578523328531 118 18 -12.918440957190949 118 19 -4.579664502164508 118 20 -4.012215007215005 118 21 1.8317866161616223 118 22 1.5033570827320872 118 23 0.947673160173153 118 24 0.621019721019735 118 25 2.2989995189995156 118 26 3.024663900913893 118 27 3.43578342953343 118 28 3.2490196608946746 118 29 2.70710678210677 118 30 2.9443891293891298 118 31 -0.312309102934087 118 32 -3.5097525853775835 118 33 0.6525264550264396 118 34 1.1369417388167307 118 35 0.5232897426647583 118 36 2.918267496392488 118 37 2.4756694925444904 118 38 1.731917388167382 118 39 2.6738392857142657 118 40 8.973503186628193 118 41 6.455156024531021 118 42 0.907252284752289 118 43 -10.340826418951423 118 44 -11.01604467291968 118 45 3.532907647907642 118 46 -6.051395202020206 118 47 -12.281460137085155 118 48 -5.716059403559399 118 49 -9.135045394420388 118 50 -5.401703342953336 118 51 -8.74075547138047 118 52 -9.322445286195286 118 53 -6.1513335738335755 118 54 -14.98635551948052 118 55 -4.845870610870612 118 56 -3.6112602212602347 119 1 -0.6723448773448837 119 2 0.5212993025493091 119 3 0.037116101491101006 119 4 0.5380026455026297 119 5 0.8914219576719518 119 6 0.5880321067821015 119 7 -0.2513726551226583 119 8 -0.6172110990860986 119 9 0.3351518157768112 119 10 0.45867694805194503 119 11 -7.126222943722944 119 12 -11.353967051467052 119 13 -18.228414201539188 119 14 -19.562457912457916 119 15 -8.648968554593559 119 16 -11.863867845117845 119 17 -15.173864237614247 119 18 -11.025583814333807 119 19 -5.258235930735935 119 20 -9.60150072150072 119 21 0.992500901875907 119 22 0.27121422558923314 119 23 1.8762445887445836 119 24 1.7103054353054503 119 25 0.0489995189995156 119 26 -0.582478956228961 119 27 0.07864057239057587 119 28 0.8918768037518205 119 29 0.6892496392496241 119 30 1.3729605579605604 119 31 1.8841194684944824 119 32 -3.420466871091868 119 33 -2.41890211640213 119 34 1.4047988816738766 119 35 1.3447183140933276 119 36 3.1325532106782035 119 37 2.261383778258775 119 38 2.6426316738816666 119 39 3.3524107142856963 119 40 8.616360329485339 119 41 2.0444417388167366 119 42 -4.985604858104853 119 43 -6.733683561808565 119 44 -7.01604467291968 119 45 -2.3956637806637886 119 46 -15.051395202020206 119 47 -8.335031565656582 119 48 -13.483916546416541 119 49 -10.70647396584896 119 50 -5.365989057239052 119 51 -3.6871840428090437 119 52 -4.233159571909571 119 53 -7.31204785954786 119 54 -15.682784090909092 119 55 -4.82801346801347 119 56 -1.6648316498316618 120 1 -0.4973448773448865 120 2 0.846299302549312 120 3 1.2371161014911038 120 4 0.8630026455026325 120 5 -0.3835780423280468 120 6 0.9130321067821043 120 7 0.5736273448773446 120 8 0.5827889009139042 120 9 0.660151815776814 120 10 -4.416323051948055 120 11 -1.8262229437229465 120 12 -5.078967051467053 120 13 -13.028414201539189 120 14 -13.387457912457915 120 15 -13.048968554593557 120 16 -15.488867845117845 120 17 -12.898864237614248 120 18 -8.775583814333807 120 19 -5.608235930735937 120 20 -8.92650072150072 120 21 -2.2824990981240916 120 22 -1.7537857744107654 120 23 -3.023755411255415 120 24 -3.0646945646945483 120 25 -1.101000481000483 120 26 2.167521043771039 120 27 1.9786405723905744 120 28 2.3918768037518205 120 29 0.764249639249627 120 30 2.3729605579605604 120 31 0.45911946849448526 120 32 -9.39546687109187 120 33 1.4810978835978688 120 34 1.6797988816738751 120 35 1.4947183140933262 120 36 3.032553210678202 120 37 3.1613837782587737 120 38 2.817631673881664 120 39 2.9024107142856934 120 40 7.116360329485339 120 41 -10.630558261183264 120 42 -16.635604858104855 120 43 -20.283683561808566 120 44 -17.81604467291968 120 45 -13.49566378066379 120 46 -18.851395202020207 120 47 -19.110031565656584 120 48 -15.258916546416541 120 49 -19.90647396584896 120 50 -16.71598905723905 120 51 -15.912184042809043 120 52 -16.40815957190957 120 53 -14.63704785954786 120 54 -17.957784090909094 120 55 -15.50301346801347 120 56 -14.039831649831662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 117.0 5 2.0 6 2.0 7 11.0 8 10.0 9 15.0 10 12.0 11 11.0 12 10.0 13 7.0 14 16.0 15 16.0 16 19.0 17 26.0 18 26.0 19 30.0 20 34.0 21 50.0 22 83.0 23 82.0 24 95.0 25 117.0 26 129.0 27 157.0 28 159.0 29 218.0 30 249.0 31 327.0 32 352.0 33 367.0 34 463.0 35 550.0 36 701.0 37 858.0 38 910.0 39 712.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.16510724053549 0.6477616237224701 0.07197351374694112 0.1151576219951058 8 98.80523967180078 0.9212609759608464 0.2447099467395998 0.02878940549877645 9 96.97711242262848 1.8137325464229166 0.6765510292212465 0.5326040017273643 10 32.17216064488269 43.71671224989204 14.625017993378437 9.48610911184684 11 27.896933928314382 33.95710378580682 22.354973369799914 15.790988916078883 12 38.520224557362894 17.01453864977688 18.65553476320714 25.809702029653085 13 25.007198387561186 18.16873020443421 26.216527497840485 30.607543910164125 14 22.758025046782784 25.061177486684898 26.414279545127396 25.766517921404926 15 25.292334343871808 22.101920023098025 26.201818969250755 26.403926663779416 16 24.34106294109175 26.098228431513753 27.07763214748668 22.483076479907822 17 26.84612062760904 24.15431121347344 22.052684612062762 26.946883546854757 18 22.80120915503095 29.019720742766665 23.693680725493017 24.48538937670937 19 25.191059841384284 29.28622927180966 21.427541456380677 24.09516943042538 20 22.95176385889129 28.120950323974082 23.959683225341973 24.967602591792655 21 24.75888872894775 25.00359867568735 24.49978407945876 25.737728515906145 22 23.261839643011374 28.832589607024616 23.477760184252194 24.42781056571182 23 25.593781488412265 27.450698143083347 26.802936519360877 20.152583849143515 24 21.40800460696804 29.642959976965162 26.259717823207602 22.689317592859197 25 23.99596948323017 28.08406506405643 28.81819490427523 19.101770548438175 26 21.64963293507989 27.292356412840075 30.416006909457323 20.642003742622713 27 22.001153402537486 24.149365628604382 35.27970011534025 18.569780853517877 28 20.72396884914912 24.819728872223827 34.64089991346986 19.815402365157198 29 18.807603686635947 26.32488479262673 37.84562211981567 17.02188940092166 30 16.400287976961845 26.724262059035276 40.70554355651548 16.1699064074874 31 13.421658986175114 27.534562211981566 44.75806451612903 14.285714285714285 32 14.74275832252486 24.62890906470673 47.16817985300475 13.460152759763655 33 15.406809001731103 23.413156376226198 47.80727062896711 13.372763993075592 34 12.993373667530971 23.8692019590896 52.21838087006626 10.919043503313167 35 13.106726199049401 25.997407460751838 51.40429209275529 9.491574247443468 36 13.55517142033996 24.15730337078652 50.20167098818784 12.08585422068568 37 12.75429230991199 24.42648968402828 50.32462848073871 12.494589525321022 38 13.780153112812366 25.610284558717318 51.076123068034086 9.533439260436227 39 11.266589728794 24.23542989036353 50.15868436237738 14.33929601846509 40 10.479779678214234 20.307290911726337 45.165966082040875 24.046963328018553 41 9.180708890180128 18.768158047646715 41.7925624636839 30.25857059848925 42 11.399337080270932 25.378296584522264 52.111255224095686 11.11111111111111 43 9.34795152914022 21.393537218695904 51.29832660126947 17.960184650894405 44 10.552982049797336 23.422119281991893 48.320787492762015 17.704111175448755 45 9.940845476843169 26.63396335305151 50.44005194055692 12.985139229548407 46 11.005336795038223 26.20799076878696 49.6466176258474 13.14005481032742 47 11.087113030957523 26.695464362850974 51.0007199424046 11.216702663786897 48 10.952586828073208 28.837008214440118 50.252197723014845 9.958207234471827 49 9.4356005788712 21.852387843704776 45.39797395079595 23.314037626628075 50 10.201729106628243 27.29106628242075 50.96541786743516 11.54178674351585 51 10.54456160624007 29.524772497472195 49.28499205546728 10.645673840820454 52 10.489913544668587 29.841498559077813 48.544668587896254 11.123919308357348 53 11.33054634568257 30.142712988323485 49.32968141848061 9.197059247513334 54 9.522435435002166 28.264319722983693 46.35694704948781 15.85629779252633 55 12.801270024534563 29.138403810073605 48.21763602251407 9.84269014287776 56 12.63340063455437 29.83847706951255 47.43293914046726 10.09518315546582 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.5 9 7.0 10 11.0 11 15.0 12 30.0 13 57.0 14 69.0 15 93.0 16 117.0 17 131.5 18 146.0 19 160.5 20 175.0 21 244.5 22 302.5 23 291.0 24 360.0 25 429.0 26 479.0 27 529.0 28 531.5 29 520.5 30 507.0 31 445.5 32 384.0 33 321.5 34 259.0 35 214.5 36 170.0 37 136.0 38 109.5 39 117.0 40 131.5 41 146.0 42 139.0 43 132.0 44 135.0 45 138.0 46 143.5 47 160.5 48 172.0 49 178.0 50 184.0 51 196.0 52 208.0 53 207.0 54 219.5 55 233.0 56 225.5 57 218.0 58 223.5 59 229.0 60 197.0 61 165.0 62 159.0 63 139.5 64 126.0 65 111.5 66 97.0 67 80.5 68 64.0 69 55.0 70 46.0 71 37.5 72 23.0 73 17.0 74 14.0 75 11.0 76 10.5 77 10.0 78 6.5 79 2.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.014394702749388226 14 0.0 15 0.2878940549877645 16 0.0575788109975529 17 0.0 18 0.0 19 0.1727364329926587 20 0.02878940549877645 21 0.0 22 0.0 23 0.0 24 0.014394702749388226 25 0.0 26 0.0 27 0.15834173024327047 28 0.18713113574204693 29 0.043184108248164675 30 0.02878940549877645 31 0.043184108248164675 32 0.1151576219951058 33 0.21592054124082338 34 0.07197351374694112 35 0.0575788109975529 36 0.07197351374694112 37 0.2303152439902116 38 0.3454728659853174 39 0.21592054124082338 40 0.6909457319706348 41 0.9068662732114583 42 0.1151576219951058 43 0.21592054124082338 44 0.5613934072261408 45 0.2303152439902116 46 0.20152583849143516 47 0.02878940549877645 48 0.1151576219951058 49 0.5326040017273643 50 0.10076291924571758 51 0.3454728659853174 52 0.10076291924571758 53 0.14394702749388225 54 0.2303152439902116 55 0.25910464948898804 56 0.18713113574204693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 6947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.23607312508997 #Duplication Level Percentage of deduplicated Percentage of total 1 88.41047721564405 70.93709514898518 2 6.925008970218874 11.11271052252771 3 2.224614280588446 5.354829422772419 4 0.7714388231072838 2.4758888728947746 5 0.5920344456404736 2.375125953649057 6 0.3408683171869394 1.6409961134302578 7 0.2870470039468963 1.6122067079314812 8 0.0897021887334051 0.575788109975529 9 0.1076426264800861 0.7773139484669641 >10 0.2511661284535343 3.1380451993666334 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.575788109975529 No Hit ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 29 0.4174463797322585 No Hit ATCTCTGGGAGTCAACAATCGACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.30228875773715275 No Hit ATCTCTGGGAAGTGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.20152583849143516 No Hit ATCTCTGGGTGGCATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.20152583849143516 No Hit ATCTCTGGGGGCTTTTTCGCTCTCTTGCTTCCCGCTCTCTCTTGCTTTTCTCTCTC 14 0.20152583849143516 No Hit ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTACCAAGCGC 12 0.1727364329926587 No Hit ATCTCTGGGATGTAACGAGAGCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1727364329926587 No Hit ATCTCTGGGAAGCAGTGTAGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.15834173024327047 No Hit ATCTCTGGGAAGTGGTATCAACGCAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.15834173024327047 No Hit ATCTCTGGGGACCGCAGAGCCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.14394702749388225 No Hit ATCTCTGGGGGTGCTATCTCTGGGAAGTTCAACGTCAGATCGACTTTTTTTTTTTT 10 0.14394702749388225 No Hit ATCTCTGGGGTATCCTCATCCCAAACTCTCTCTGAGCTGATACCCGAAGAGCCAGG 10 0.14394702749388225 No Hit ATCTCTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.14394702749388225 No Hit ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 9 0.12955232474449402 No Hit ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.12955232474449402 No Hit ATCTCTGGGAAGCATGGCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 9 0.12955232474449402 No Hit ATCTCTGGGTGCCGCAGAGTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.12955232474449402 No Hit ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTT 9 0.12955232474449402 No Hit ATCTCTGGGATGTCACCAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.12955232474449402 No Hit ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 8 0.1151576219951058 No Hit ATCTCTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC 8 0.1151576219951058 No Hit ATCTCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 8 0.1151576219951058 No Hit ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 8 0.1151576219951058 No Hit ATCTCTGGGATCCGCAGAGTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.1151576219951058 No Hit ATCTCTGGGATGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGAAGCATGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 7 0.10076291924571758 No Hit ATCTCTGGGAACATGGTATAACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG 7 0.10076291924571758 No Hit ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 7 0.10076291924571758 No Hit ATCTCTGGGAAGCATGTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 7 0.10076291924571758 No Hit ATCTCTGGGCATGTAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGTTACGCAGACGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGGTATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGAGTGTTACGCAGAGTCGACTTTTTTTTTTTTCTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGTACCGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGAACGCAGAGTCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit ATCTCTGGGATCGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGT 7 0.10076291924571758 No Hit ATCTCTGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10076291924571758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.014394702749388226 0.0 0.0 0.0 0.0 11 0.014394702749388226 0.0 0.0 0.0 0.0 12 0.014394702749388226 0.0 0.0 0.0 0.0 13 0.014394702749388226 0.0 0.0 0.0 0.0 14 0.014394702749388226 0.0 0.0 0.0 0.0 15 0.014394702749388226 0.0 0.0 0.0 0.0 16 0.014394702749388226 0.0 0.0 0.0 0.0 17 0.014394702749388226 0.0 0.0 0.0 0.0 18 0.02878940549877645 0.0 0.0 0.0 0.0 19 0.02878940549877645 0.0 0.0 0.0 0.0 20 0.02878940549877645 0.0 0.0 0.0 0.0 21 0.02878940549877645 0.0 0.0 0.0 0.0 22 0.02878940549877645 0.0 0.0 0.0 0.0 23 0.02878940549877645 0.0 0.0 0.0 0.0 24 0.02878940549877645 0.0 0.0 0.0 0.0 25 0.043184108248164675 0.0 0.0 0.0 0.0 26 0.10076291924571758 0.0 0.0 0.0 0.0 27 0.12955232474449402 0.0 0.0 0.0 0.0 28 0.2303152439902116 0.0 0.0 0.0 0.0 29 0.2303152439902116 0.0 0.0 0.0 0.0 30 0.2303152439902116 0.0 0.0 0.0 0.0 31 0.2303152439902116 0.0 0.0 0.0 0.0 32 0.2303152439902116 0.0 0.0 0.0 0.0 33 0.2303152439902116 0.0 0.0 0.0 0.0 34 0.2303152439902116 0.0 0.0 0.0 0.0 35 0.24470994673959984 0.0 0.0 0.0 0.0 36 0.25910464948898804 0.0 0.0 0.0 0.0 37 0.25910464948898804 0.0 0.0 0.0 0.0 38 0.25910464948898804 0.0 0.0 0.0 0.0 39 0.2734993522383763 0.0 0.0 0.0 0.0 40 0.2734993522383763 0.0 0.0 0.0 0.0 41 0.2734993522383763 0.0 0.0 0.0 0.0 42 0.2734993522383763 0.0 0.0 0.0 0.0 43 0.30228875773715275 0.0 0.0 0.0 0.0 44 0.30228875773715275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGGAG 45 1.0186341E-10 49.869568 6 TGGGAGT 45 1.0186341E-10 49.869568 6 GGATGTC 15 0.008166363 49.869564 8 CAGCAAC 15 0.008166363 49.869564 21 CTGGGTG 25 1.9070752E-5 49.869564 5 CTGGGGG 60 0.0 49.869564 5 CTGGGGA 60 0.0 49.869564 5 CTGGGCT 20 3.949884E-4 49.869564 5 GAAGCAT 25 1.9070752E-5 49.869564 9 GGGAGTG 20 3.949884E-4 49.869564 7 ATCAGCA 15 0.008166363 49.869564 19 TCAGCAA 15 0.008166363 49.869564 20 TGGGGGG 25 1.9070752E-5 49.869564 6 TGGGGGC 15 0.008166363 49.869564 6 TGGGGCT 15 0.008166363 49.869564 6 TCGTCTT 15 0.008166363 49.869564 11 GTCTTAA 15 0.008166363 49.869564 13 TAATCAG 15 0.008166363 49.869564 17 GGGATGT 15 0.008166363 49.869564 7 GGGATGG 20 3.949884E-4 49.869564 7 >>END_MODULE