FastQCFastQC Report
Fri 17 Jun 2016
SRR1526197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26211
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.179314028461333No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.14116210751211322No Hit
ATGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA300.11445576284765938No Hit
ATGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA270.10301018656289344No Hit
ATGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10301018656289344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCA150.00802663450.48449738
TCGTCTG203.954577E-450.3868535
CCAAAAC203.9926107E-450.28957743
GCAGCAT150.00821322350.19267742
ACATGGT301.0357726E-649.80879211
AGCAGTT150.00846700449.80879211
AGAGTGT150.00846700449.80879211
GCGGGAT150.00853135149.713744
GCGGGAA650.049.713744
GGGTCTT301.049586E-649.713748
GGGTTTC150.00853135149.713748
GGGTCGA150.00853135149.713748
GACAGTG150.00853135149.713749
GAGTGGT204.2269618E-449.713749
CGGGGTC204.2269618E-449.713745
GGGTACA204.2269618E-449.713747
GAAGAGT150.00853135149.713749
GAACTGG204.2269618E-449.713749
GAACCCC150.00853135149.713749
CGGGATC150.00853135149.713745
Produced by FastQC (version 0.11.5)