##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526197_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26211 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.157033306626985 38.0 38.0 39.0 35.0 39.0 2 36.424134905192474 38.0 38.0 39.0 34.0 39.0 3 35.98641791614208 38.0 35.0 39.0 31.0 39.0 4 36.01091144939148 38.0 35.0 39.0 31.0 39.0 5 36.30834382511159 38.0 36.0 39.0 33.0 39.0 6 36.29548662775171 38.0 36.0 39.0 33.0 39.0 7 36.5670138491473 38.0 37.0 39.0 35.0 39.0 8 35.14955553012094 35.0 35.0 39.0 32.0 39.0 9 35.016443477929116 35.0 35.0 39.0 31.0 39.0 10 33.49738659341498 35.0 32.0 37.0 26.0 39.0 11 32.51047270230056 35.0 30.0 38.0 23.0 39.0 12 30.71985044446988 35.0 28.0 37.0 16.0 38.0 13 30.752928159932853 35.0 28.0 38.0 15.0 38.0 14 31.16573194460341 35.0 29.0 38.0 17.0 38.0 15 31.148449124413414 35.0 29.0 38.0 16.0 38.0 16 29.92766395788028 33.0 26.0 37.0 15.0 38.0 17 30.048033268475066 33.0 27.0 36.0 17.0 38.0 18 29.63217733012857 33.0 26.0 36.0 14.0 38.0 19 30.273129602075464 33.0 27.0 36.0 18.0 38.0 20 29.86990194956316 33.0 26.0 36.0 17.0 38.0 21 30.583037655945976 33.0 27.0 38.0 17.0 38.0 22 30.415817786425546 33.0 27.0 37.0 17.0 38.0 23 30.387051238029834 33.0 27.0 37.0 18.0 38.0 24 30.18538018389226 33.0 27.0 36.0 16.0 38.0 25 30.283735836099346 33.0 27.0 36.0 17.0 38.0 26 30.730304070809964 34.0 29.0 38.0 16.0 39.0 27 30.67429705085651 34.0 29.0 38.0 16.0 39.0 28 30.72759528442257 34.0 29.0 38.0 16.0 39.0 29 30.442867498378543 34.0 28.0 38.0 15.0 39.0 30 30.2615314181069 34.0 28.0 38.0 14.0 39.0 31 30.2884285223761 35.0 28.0 38.0 10.0 39.0 32 30.104955934531304 35.0 28.0 38.0 10.0 39.0 33 30.223112433711037 35.0 28.0 38.0 10.0 39.0 34 30.024112014039908 35.0 28.0 38.0 9.0 39.0 35 29.859753538590667 35.0 28.0 37.0 4.0 39.0 36 29.375414902140324 35.0 28.0 36.0 4.0 39.0 37 29.092632864064704 34.0 27.0 36.0 4.0 39.0 38 28.728777993971995 33.0 26.0 36.0 4.0 39.0 39 28.576361069779864 33.0 26.0 36.0 4.0 38.0 40 25.57548357559803 31.0 14.0 35.0 4.0 38.0 41 22.84395864331769 28.0 9.0 35.0 4.0 38.0 42 26.39567357216436 31.0 20.0 35.0 4.0 38.0 43 25.315096715119605 31.0 15.0 35.0 4.0 38.0 44 25.170233871275418 31.0 13.0 35.0 4.0 38.0 45 25.83434435923849 31.0 18.0 35.0 4.0 38.0 46 25.42440196863912 32.0 11.0 35.0 4.0 38.0 47 24.99855022700393 31.0 8.0 35.0 4.0 38.0 48 25.005188661249093 31.0 9.0 35.0 4.0 38.0 49 23.04746098966083 29.0 4.0 35.0 4.0 38.0 50 24.589409026744498 31.0 4.0 35.0 4.0 38.0 51 24.741482583648086 32.0 4.0 35.0 4.0 39.0 52 24.40547861584831 32.0 4.0 35.0 4.0 39.0 53 24.04452329174774 31.0 4.0 35.0 4.0 39.0 54 23.23532104841479 30.0 4.0 35.0 4.0 39.0 55 23.385029186219526 31.0 4.0 35.0 4.0 39.0 56 22.772461943458854 30.0 4.0 35.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6876276043238647 1 2 1.9290424986153596 1 3 2.278692412016774 1 4 2.4376996386138146 1 5 1.9790411694523158 1 6 2.2545890265005184 1 7 1.9977705387100002 1 8 1.673199012807224 1 9 1.665152946146378 1 10 1.7980216686993913 1 11 -3.5980938950334433 1 12 -13.687738931945745 1 13 -6.993563443527709 1 14 -6.824004049006422 1 15 -6.379217119621774 1 16 -20.657851701000578 1 17 -3.910023138716504 1 18 -10.44157729837925 1 19 -4.77405029321438 1 20 -4.713234515804295 1 21 -3.9433774997617093 1 22 -3.3255576640555233 1 23 -4.395040052705408 1 24 -4.271888423662528 1 25 -3.5990302224632096 1 26 2.7393155176255704 1 27 3.7807335845418315 1 28 4.1664730282616596 1 29 2.8599314941238134 1 30 4.086798813175694 1 31 1.483256616399082 1 32 2.1242247163107812 1 33 2.846224526987431 1 34 3.5468978651640093 1 35 1.7276476521950208 1 36 2.246439280761411 1 37 1.3656968993221454 1 38 1.4247256322776067 1 39 2.0025843890785566 1 40 3.5354405961568034 1 41 -7.788320203692367 1 42 -8.746576725269517 1 43 -13.420688200580175 1 44 -18.340816201580704 1 45 -10.03474422777385 1 46 -12.238427305362473 1 47 -19.204781938760984 1 48 -11.42537750632034 1 49 -16.827288259064215 1 50 -10.10284366857559 1 51 -7.412791731079466 1 52 -6.720856407380051 1 53 -7.589338528909471 1 54 -6.707677749113204 1 55 -7.05275959191504 1 56 -4.123809484427358 2 1 0.8833405586575083 2 2 2.1145038220077197 2 3 1.7567912004603912 2 4 1.0947359853053342 2 5 1.3928342729005934 2 6 1.240609529762402 2 7 1.2661768760818504 2 8 1.3293033930495355 2 9 0.9382191157642765 2 10 0.5631661234990233 2 11 0.9499023771007629 2 12 -2.6234332469504054 2 13 -2.3374590632853973 2 14 2.718400424432531 2 15 1.398042899017554 2 16 0.024347739819553738 2 17 1.2223160970710083 2 18 -6.20112995448363 2 19 0.8242721671770461 2 20 1.3771662297688643 2 21 1.6810400212075365 2 22 0.7722988131112984 2 23 0.9553793321967348 2 24 -2.453621881258055 2 25 -0.41450086552006127 2 26 0.9294366732639681 2 27 0.9438277131532011 2 28 0.1953639881871041 2 29 1.0845354083829015 2 30 -0.06231581869755942 2 31 -1.220378052752828 2 32 -1.5458591606696501 2 33 -2.371855622127203 2 34 -2.505292255991627 2 35 -3.6637782564722663 2 36 -1.9110630491547091 2 37 -1.1869592050580948 2 38 -3.2155353183281683 2 39 -2.080360624900937 2 40 -1.515817558549628 2 41 -7.110780595118275 2 42 -11.359810648848265 2 43 -13.063745050533576 2 44 -14.443332510993567 2 45 -9.28730713550917 2 46 -11.560887696788381 2 47 -14.518854632144022 2 48 -10.238052250029567 2 49 -11.783485835951447 2 50 -10.06463304415807 2 51 -8.703565263232308 2 52 -8.294947740092073 2 53 -8.74031709368114 2 54 -7.310660041750669 2 55 -7.869162201421098 2 56 -6.235645458332298 3 1 0.8833405586575083 3 2 2.0604497679536635 3 3 2.027061470730658 3 4 1.5812224717918184 3 5 2.0685099485762706 3 6 2.105474394627265 3 7 1.914825524730503 3 8 1.9779520416981882 3 9 1.4247056022507607 3 10 1.887490447823346 3 11 2.436388863587247 3 12 3.9171072935901385 3 13 1.0409193150929816 3 14 1.7184004244325308 3 15 1.4520969530716101 3 16 2.6189423344141467 3 17 3.6277215024764153 3 18 2.9610322076785316 3 19 1.9864343293392075 3 20 2.458247310849945 3 21 1.9242832644507786 3 22 2.20473124554373 3 23 1.7121360889534927 3 24 1.1139456863095134 3 25 0.6936072425880475 3 26 1.5240312678585646 3 27 0.3222060915315801 3 28 0.14130993413305148 3 29 0.7602110840585787 3 30 0.5322787758970371 3 31 -1.1392969716717474 3 32 0.102789487978999 3 33 1.1416578913863091 3 34 0.6298428791435065 3 35 0.849735257041246 3 36 1.710558572466912 3 37 2.3806083625094736 3 38 3.297978195185344 3 39 3.108828564288249 3 40 4.835533792801723 3 41 8.429759945422266 3 42 3.5050542160165996 3 43 0.42274143595291136 3 44 3.610721543060489 3 45 4.8478279996259666 3 46 0.817490681589998 3 47 -3.7350708483602375 3 48 0.8160018040244879 3 49 4.0543520018863894 3 50 2.7461777666527425 3 51 1.3234617637947181 3 52 1.1104576653133336 3 53 1.6110342576702124 3 54 2.689339958249331 3 55 1.6984053661464706 3 56 2.3859761632893246 4 1 1.505590715791385 4 2 2.7606383286073424 4 3 2.678852796940589 4 4 3.29335320717837 4 5 2.3928342729005934 4 6 2.6347012959471883 4 7 2.681010314171104 4 8 2.777449213288378 4 9 2.547270027140769 4 10 3.793210120984881 4 11 3.5061563054477105 4 12 5.670721372785611 4 13 4.622314663930194 4 14 5.49275617553247 4 15 4.919098838678149 4 16 5.32290210814136 4 17 6.417162105870506 4 18 6.257700969463574 4 19 5.696679458188992 4 20 4.382194513866917 4 21 4.334088418441976 4 22 4.269470403306148 4 23 4.318672858280959 4 24 4.878873404725606 4 25 4.03930177432909 4 26 3.467463071755482 4 27 3.5786485805322066 4 28 3.7157913923354364 4 29 3.2272129696651177 4 30 2.3041203849479466 4 31 3.4992951087807995 4 32 2.6791565527181582 4 33 2.9480689536113296 4 34 2.607844136214535 4 35 1.998698173446023 4 36 4.677874725829575 4 37 4.085825207261202 4 38 3.5361931543305367 4 39 5.011405559888505 4 40 7.487325119011654 4 41 9.784253974334899 4 42 6.202100099108993 4 43 7.622615728850459 4 44 6.214115634826673 4 45 6.683780230927034 4 46 6.938798035455491 4 47 6.107166738063395 4 48 8.118327385419839 4 49 9.69797236643699 4 50 7.867485120518236 4 51 7.870287659457823 4 52 7.414668853245452 4 53 7.590292585765749 4 54 9.390157054415265 4 55 6.531843455398512 4 56 4.498484019983227 5 1 1.3954315970643378 5 2 2.411801119305018 5 3 1.8385835190948114 5 4 1.5357032683778797 5 5 1.2349395360584907 5 6 1.5649338540867248 5 7 1.3707287964232435 5 8 1.6550501924805445 5 9 1.2767681911554547 5 10 0.8697095089897715 5 11 2.2258625477977745 5 12 4.379411703262964 5 13 4.1461824729877215 5 14 2.9609324301224333 5 15 3.0182420455324923 5 16 3.6288996601609433 5 17 3.7941510899586355 5 18 3.1836495618748337 5 19 2.4010858229380716 5 20 3.027237353524196 5 21 2.2998166926157886 5 22 3.8441338059989256 5 23 2.4361759182564775 5 24 2.99943644022132 5 25 2.951786474451488 5 26 1.7765205992952637 5 27 1.5547807714746824 5 28 2.1619358231799914 5 29 2.352671965993146 5 30 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4.241519511011266 6 18 4.420491667137991 6 19 3.6905595071485955 6 20 3.527237353524196 6 21 2.0892903768263125 6 22 2.9230811744199734 6 23 2.725649602467005 6 24 3.39417328232658 6 25 4.451786474451488 6 26 2.0923100729794726 6 27 1.0021491925273125 6 28 2.451409507390519 6 29 1.0368824923089335 6 30 2.239248903919794 6 31 0.3144725873609673 6 32 1.8474552063289273 6 33 1.350761732069099 6 34 0.6213080285033925 6 35 1.8120396667140781 6 36 3.274569952267761 6 37 3.1188729428793174 6 38 3.3122029462521994 6 39 3.191332120476016 6 40 7.278634788534298 6 41 3.2597741701733334 6 42 4.08826900975771 6 43 3.6709633420695518 6 44 5.2771511305427055 6 45 5.093204955529238 6 46 4.204403910608491 6 47 4.441316064868779 6 48 4.947580751392909 6 49 4.447666368884967 6 50 4.475196258829129 6 51 3.7423806827136374 6 52 3.8344974946163184 6 53 3.515017187968933 6 54 3.773265989543784 6 55 3.3292730759615488 6 56 4.70105439942019 7 1 1.2887459640629118 7 2 1.9523416598455583 7 3 2.4324668761360613 7 4 2.3920332826026325 7 5 2.1495910296573513 7 6 2.2135825027353704 7 7 2.1850957950007697 7 8 2.2211952849414303 7 9 2.019300196845357 7 10 2.1577607180936127 7 11 3.382334809533191 7 12 5.241431617914461 7 13 4.230108504282168 7 14 5.285967992000103 7 15 6.343988844963501 7 16 5.645969361441171 7 17 5.843937718692633 7 18 5.177248423894749 7 19 6.472920815825692 7 20 5.02581487841751 7 21 4.870229210396726 7 22 4.907433948246432 7 23 4.225649602467005 7 24 3.8436754160392432 7 25 4.7476612966421 7 26 4.3618691056964 7 27 3.7276114969369836 7 28 4.465634258457374 7 29 4.19264351649101 7 30 4.3971436407619 7 31 4.0498922175174386 7 32 5.535221920411434 7 33 4.95246870219712 7 34 3.981194230494861 7 35 4.579464986770976 7 36 3.5754234373317786 7 37 3.4616894435905508 7 38 4.270951168158319 7 39 5.000720456180144 7 40 6.267966225234154 7 41 1.618949134611455 7 42 2.9645136754760593 7 43 4.747065760277234 7 44 2.745856678195622 7 45 2.280260432058398 7 46 2.8985717626710787 7 47 3.9946588813694923 7 48 3.0051909932136773 7 49 5.0543520018863894 7 50 4.097529118004093 7 51 4.891029331362287 7 52 4.407754962610628 7 53 5.8542775009134544 7 54 6.040691309600682 7 55 5.292999960741064 7 56 5.494084271397433 8 1 1.0428000181169708 8 2 2.5118011193050194 8 3 2.9148993085684936 8 4 2.2764927420620893 8 5 2.092834272900596 8 6 2.7149338540867234 8 7 2.1891498490548287 8 8 2.232681771427913 8 9 2.728083980629137 8 10 2.3118147721476703 8 11 3.01138886358725 8 12 4.734674861157703 8 13 4.6159193150929845 8 14 4.809616640648748 8 15 6.2656104665851196 8 16 5.297320712792523 8 17 4.9270458268007395 8 18 5.345491667137988 8 19 4.193191086095968 8 20 5.229868932471565 8 21 4.569553534721049 8 22 2.4520285428410276 8 23 4.1256496024670035 8 24 3.4625943349581654 8 25 3.4044180533988566 8 26 4.543625862453155 8 27 3.585043929369416 8 28 3.1500937179168353 8 29 3.473724597572094 8 30 1.6721436407618988 8 31 0.8539462715714947 8 32 2.3750867852762987 8 33 1.2876038373322558 8 34 0.6805185548191837 8 35 2.2923028246088144 8 36 2.867991004899345 8 37 2.984662416563527 8 38 2.6635187357258836 8 39 2.993963699423386 8 40 4.623371630639561 8 41 -2.2296995140371934 8 42 3.2422163781787603 8 43 3.8801738683853415 8 44 3.1850458673848117 8 45 3.1721523239502893 8 46 2.1701933842926984 8 47 3.367631854342463 8 48 3.641001804024487 8 49 5.725297947832338 8 50 2.4488804693554442 8 51 3.3173806827136367 8 52 3.7239711788268473 8 53 4.087385609021563 8 54 4.32852914743852 8 55 4.371378339119442 8 56 3.558949136262296 9 1 1.314228589545536 9 2 2.7546582621621596 9 3 2.322042165711352 9 4 2.3943498849192366 9 5 2.3356914157577364 9 6 2.393505282658154 9 7 2.039149849054823 9 8 3.079110342856488 9 9 2.8316554092005717 9 10 2.5832433435762425 9 11 3.8649602921586776 9 12 5.627532004014849 9 13 4.812347886521554 9 14 4.8774737835058914 9 15 5.251324752299407 9 16 5.140177855649672 9 17 5.466331541086451 9 18 5.734777381423708 9 19 4.743191086095965 9 20 6.051297503900134 9 21 4.337410677578195 9 22 5.130599971412455 9 23 4.339935316752719 9 24 6.076880049243879 9 25 4.732989481970286 9 26 4.290054433881728 9 27 3.4743296436551354 9 28 2.696522289345406 9 29 3.5594388832863757 9 30 3.9114293550476127 9 31 3.4468034144286364 9 32 5.0858010709905805 9 33 4.1447466944751135 9 34 4.44837569767633 9 35 4.817302824608813 9 36 5.400133862042203 9 37 5.29180527370638 9 38 6.324233021440172 9 39 5.129677985137672 9 40 8.587657344925272 9 41 5.8845862002485205 9 42 6.467216378178762 9 43 7.969459582671057 9 44 7.877903010241955 9 45 6.772152323950291 9 46 6.634479098578414 9 47 6.110488997199607 9 48 7.730287518310202 9 49 8.400297947832335 9 50 7.506023326498301 9 51 6.728094968427921 9 52 7.566828321683989 9 53 6.448099894735847 9 54 7.392814861724236 9 55 5.09994976769087 9 56 6.244663421976583 10 1 1.4197230950400481 10 2 2.6169293244332223 10 3 2.582848026517212 10 4 2.74892863949799 10 5 2.3671932472595643 10 6 2.667497956650827 10 7 2.264790874695855 10 8 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5.664605692247626 119 39 4.765702829858167 119 40 6.716849891509128 119 41 -2.4644821227328464 119 42 -6.550174926169063 119 43 -13.362217435962483 119 44 -12.6736497847891 119 45 -13.653934632571449 119 46 -17.39285009396817 119 47 -15.641063797831448 119 48 -15.705737326410295 119 49 -14.956223791298097 119 50 -14.551119530644556 119 51 -13.583706273808101 119 52 -13.115159255955763 119 53 -13.298483956195827 119 54 -14.338862156909306 119 55 -12.502534704358819 119 56 -11.597572602868139 120 1 1.092800018116968 120 2 1.711801119305015 120 3 2.264899308568495 120 4 2.601492742062092 120 5 1.8428342729005962 120 6 2.1649338540867262 120 7 1.8891498490548244 120 8 1.2076817714279144 120 9 1.1030839806291368 120 10 0.6118147721476674 120 11 -2.7136111364127515 120 12 -8.565325138842297 120 13 -7.55908068490702 120 14 -9.765383359351251 120 15 -11.484389533414877 120 16 -11.652679287207473 120 17 -13.097954173199263 120 18 -13.679508332862008 120 19 -6.006808913904035 120 20 -8.870131067528433 120 21 -5.305446465278951 120 22 -3.3979714571589703 120 23 -4.574350397532996 120 24 -6.637405665041836 120 25 -2.3455819466011434 120 26 2.018625862453156 120 27 2.26004392936942 120 28 0.6750937179168339 120 29 -0.476275402427909 120 30 2.6471436407619002 120 31 -1.946053728428506 120 32 -6.749913214723701 120 33 -0.31239616266774206 120 34 -0.994481445180817 120 35 -2.182697175391187 120 36 0.792991004899342 120 37 1.4346624165635262 120 38 1.8385187357258843 120 39 1.2439636994233858 120 40 3.023371630639563 120 41 -3.529699514037194 120 42 -7.93278362182124 120 43 -11.444826131614658 120 44 -11.664954132615188 120 45 -10.77784767604971 120 46 -16.829806615707298 120 47 -16.482368145657535 120 48 -14.583998195975513 120 49 -16.199702052167662 120 50 -14.101119530644556 120 51 -14.257619317286363 120 52 -13.826028821173153 120 53 -14.187614390978437 120 54 -15.52147085256148 120 55 -13.428621660880557 120 56 -11.891050863737703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 476.0 5 15.0 6 29.0 7 52.0 8 100.0 9 150.0 10 97.0 11 143.0 12 103.0 13 96.0 14 146.0 15 171.0 16 212.0 17 273.0 18 337.0 19 367.0 20 463.0 21 481.0 22 548.0 23 593.0 24 633.0 25 718.0 26 710.0 27 951.0 28 1144.0 29 1341.0 30 1621.0 31 1908.0 32 2138.0 33 2318.0 34 2403.0 35 2004.0 36 1600.0 37 1262.0 38 531.0 39 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.87451833199802 0.8698637976422113 0.179314028461333 0.07630384189843958 8 98.42432566479722 1.2475678150394873 0.179314028461333 0.14879249170195719 9 93.87280149555531 4.322612643546603 0.7859295715539277 1.0186562893441684 10 36.54572507725764 43.779329289229715 10.587158063408493 9.087787570104155 11 31.696615924611805 31.39521574911297 17.92377246194346 18.98439586433177 12 33.58895120369311 17.13402769829461 22.086147037503338 27.190874060508946 13 21.520146520146522 19.608516483516482 26.343101343101345 32.528235653235654 14 26.90854984548472 21.62069360192286 24.99332341383389 26.47743313875854 15 26.186473060756594 18.8421857088269 25.35727932747421 29.6140619029423 16 28.071916631675382 21.460472573195403 25.415123869145322 25.052486925983892 17 29.533402006791043 22.975086795620157 22.967456411430316 24.524054786158484 18 26.820800427301517 24.8216397695624 22.769066422494372 25.588493380641715 19 27.619120342089186 25.912492364080634 22.39233353695785 24.07605375687233 20 26.395144667531873 25.620276356973815 24.143064356057714 23.841514619436598 21 26.397314104765172 21.281141505474803 27.33585136011598 24.985693029644043 22 28.850482621800005 23.623669451756896 23.7114188699401 23.814429056502995 23 26.984853687383158 27.19850444469879 23.761016367174086 22.05562550074396 24 28.251497462897255 25.493113578268666 24.90557399565068 21.349814963183398 25 25.230827928271655 26.46699732926364 27.390309042350246 20.91186570011446 26 23.00179314028461 22.448590286520925 32.52451260920987 22.025103963984584 27 27.45666946628999 18.748568374436893 32.3852790715431 21.409483087730013 28 24.80427725797212 22.287569219018522 32.75157532938706 20.156578193622302 29 22.627542259701606 22.535963673827602 35.16236120120578 19.67413286526501 30 21.823731400228922 20.31667302556276 37.851964898893556 20.007630675314765 31 18.994200244200243 23.008241758241756 39.804639804639805 18.192918192918192 32 21.378388697976327 20.78274150439099 40.756013745704465 17.082856051928218 33 20.478245922304136 22.21246036899805 42.247603040605064 15.061690668092744 34 17.553901927113145 21.511161991986263 45.02575844304522 15.90917763785537 35 17.662099759569514 21.97458306300805 46.406899973285505 13.956417204136931 36 17.841468534137313 22.459260390031677 44.781131931458226 14.918139144372782 37 19.03052064631957 22.227739791435884 44.75724817601895 13.984491386225601 38 18.664219902894065 22.98428718889781 44.71460794433612 13.636884963872003 39 18.081406459010125 22.037072424995223 44.81177145041085 15.069749665583796 40 13.987866687144832 16.856089694363384 40.80402395945323 28.352019659038554 41 12.238071436810335 15.61767080625937 37.32554115882964 34.81871659810066 42 16.087653661143772 21.344582728869206 46.17469649538062 16.3930671146064 43 14.256759340701366 19.694825805958164 44.23878542200467 21.809629431335807 44 14.389757743023612 19.81370745170193 43.648420729837476 22.148114075436983 45 17.654476670870114 22.419656845886355 45.045664717795866 14.880201765447667 46 16.282536406375417 22.531819745442036 45.27003783969728 15.915606008485264 47 15.241947794229887 21.88215539612273 45.66096779117692 17.214929018470464 48 16.790802490355603 22.779878537870974 46.193804667506974 14.235514304266452 49 14.022239263803682 18.642638036809817 40.318251533742334 27.016871165644176 50 14.788651724006263 23.62060407041124 46.11478101492993 15.475963190652564 51 17.220543806646525 23.882366438487132 43.76075566943287 15.136334085433479 52 16.070269238113426 25.60244414741264 42.76112278021768 15.566163834256253 53 16.817539436996295 25.70566441312402 42.88224284786678 14.59455330201291 54 14.480728051391864 24.54114408075864 41.60293667788314 19.37519118996635 55 16.85899885364922 25.284677111196025 42.246847535345815 15.609476499808942 56 17.949109803621916 26.270344616795292 41.296706655459616 14.483838924123177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 5.0 11 8.0 12 20.0 13 80.5 14 129.0 15 134.5 16 140.0 17 167.0 18 194.0 19 234.0 20 274.0 21 341.0 22 414.0 23 420.0 24 571.0 25 722.0 26 799.5 27 877.0 28 1050.5 29 1330.5 30 1437.0 31 1459.0 32 1481.0 33 1398.5 34 1316.0 35 1206.5 36 1097.0 37 989.5 38 730.5 39 579.0 40 503.5 41 428.0 42 426.0 43 424.0 44 412.5 45 401.0 46 423.5 47 482.0 48 518.0 49 532.5 50 547.0 51 627.5 52 708.0 53 731.0 54 795.5 55 837.0 56 883.0 57 929.0 58 990.5 59 1052.0 60 1068.0 61 1084.0 62 1164.0 63 1233.5 64 1223.0 65 1134.5 66 1046.0 67 988.0 68 930.0 69 826.0 70 722.0 71 647.0 72 470.0 73 368.0 74 313.5 75 259.0 76 233.0 77 207.0 78 181.0 79 113.0 80 71.0 81 49.0 82 27.0 83 24.0 84 21.0 85 14.5 86 8.0 87 5.5 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.011445576284765937 14 0.0 15 0.15642287589180115 16 0.05341268932890771 17 0.0 18 0.0 19 0.0724886498035176 20 0.04959749723398573 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0038151920949219794 26 0.0 27 0.06485826561367365 28 0.09919499446797146 29 0.015260768379687917 30 0.0038151920949219794 31 0.011445576284765937 32 0.08011903399336157 33 0.12208614703750334 34 0.022891152569531874 35 0.030521536759375835 36 0.030521536759375835 37 0.12208614703750334 38 0.2060203731257869 39 0.17549883636641103 40 0.6371370798519705 41 0.7706688031742398 42 0.06485826561367365 43 0.23654190988516272 44 0.46926862767540345 45 0.16023806798672313 46 0.183129220556255 47 0.026706344664453856 48 0.11445576284765938 49 0.4997901644347793 50 0.08393422608828355 51 0.23654190988516272 52 0.09919499446797146 53 0.11445576284765938 54 0.22509633360039677 55 0.15642287589180115 56 0.14116210751211322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 26211.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.48868795543855 #Duplication Level Percentage of deduplicated Percentage of total 1 89.19425066716606 72.68322460035863 2 6.606114518469966 10.766472091869826 3 2.003839131045461 4.898706649879822 4 0.8193267475069057 2.6706344664453856 5 0.46818671286108904 1.9075960474609897 6 0.2996394962310969 1.46503376445004 7 0.13109227960110492 0.7477776506047079 8 0.08427360831499602 0.5493876616687651 9 0.08427360831499602 0.6180611193773606 >10 0.30900323048831874 3.693105947884476 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.179314028461333 No Hit ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.14116210751211322 No Hit ATGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAACAAAAAAAAAAAAAAA 30 0.11445576284765938 No Hit ATGGCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.10301018656289344 No Hit ATGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.10301018656289344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.007630384189843959 0.0 0.0 0.0 0.0 12 0.007630384189843959 0.0 0.0 0.0 0.0 13 0.015260768379687917 0.0 0.0 0.0 0.0 14 0.015260768379687917 0.0 0.0 0.0 0.0 15 0.015260768379687917 0.0 0.0 0.0 0.0 16 0.019075960474609896 0.0 0.0 0.0 0.0 17 0.030521536759375835 0.0 0.0 0.0 0.0 18 0.041967113044141774 0.0 0.0 0.0 0.0 19 0.06485826561367365 0.0 0.0 0.0 0.0 20 0.06867345770859563 0.0 0.0 0.0 0.0 21 0.0724886498035176 0.0 0.0 0.0 0.0 22 0.08011903399336157 0.0 0.0 0.0 0.0 23 0.09537980237304948 0.0 0.0 0.0 0.0 24 0.10682537865781543 0.0 0.0 0.0 0.0 25 0.12208614703750334 0.0 0.0 0.0 0.0 26 0.12590133913242532 0.0 0.0 0.0 0.0 27 0.13353172332226929 0.0 0.0 0.0 0.0 28 0.14116210751211322 0.0 0.0 0.0 0.0 29 0.14879249170195719 0.0 0.0 0.0 0.0 30 0.15642287589180115 0.0 0.0 0.0 0.0 31 0.16023806798672313 0.0 0.0 0.0 0.0 32 0.1678684521765671 0.0 0.0 0.0 0.0 33 0.1678684521765671 0.0 0.0 0.0 0.0 34 0.1678684521765671 0.0 0.0 0.0 0.0 35 0.17168364427148908 0.0 0.0 0.0 0.0 36 0.17549883636641106 0.0 0.0 0.0 0.0 37 0.17931402846133304 0.0 0.0 0.0 0.0 38 0.17931402846133304 0.0 0.0 0.0 0.0 39 0.17931402846133304 0.0 0.0 0.0 0.0 40 0.183129220556255 0.0 0.0 0.0 0.0 41 0.183129220556255 0.0 0.0 0.0 0.0 42 0.19075960474609896 0.0 0.0 0.0 0.0 43 0.19457479684102094 0.0 0.0 0.0 0.0 44 0.19457479684102094 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGGCA 15 0.008026634 50.484497 38 TCGTCTG 20 3.954577E-4 50.38685 35 CCAAAAC 20 3.9926107E-4 50.289577 43 GCAGCAT 15 0.008213223 50.192677 42 ACATGGT 30 1.0357726E-6 49.808792 11 AGCAGTT 15 0.008467004 49.808792 11 AGAGTGT 15 0.008467004 49.808792 11 GCGGGAT 15 0.008531351 49.71374 4 GCGGGAA 65 0.0 49.71374 4 GGGTCTT 30 1.049586E-6 49.71374 8 GGGTTTC 15 0.008531351 49.71374 8 GGGTCGA 15 0.008531351 49.71374 8 GACAGTG 15 0.008531351 49.71374 9 GAGTGGT 20 4.2269618E-4 49.71374 9 CGGGGTC 20 4.2269618E-4 49.71374 5 GGGTACA 20 4.2269618E-4 49.71374 7 GAAGAGT 15 0.008531351 49.71374 9 GAACTGG 20 4.2269618E-4 49.71374 9 GAACCCC 15 0.008531351 49.71374 9 CGGGATC 15 0.008531351 49.71374 5 >>END_MODULE