FastQCFastQC Report
Fri 17 Jun 2016
SRR1526196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87509
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2680.30625421385228946No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2580.29482681781302494No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2220.25368819207167265No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1760.2011221702910558No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA1240.14169971088688021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC550.050.26367641
GTTAAAC204.055773E-450.2636740
ATAACCT150.00823203150.2636740
GAGTACG150.00826958950.20586436
AAGGCCA252.021706E-550.20586436
AAAGGCC252.028602E-550.17701335
TACCAAG150.00830727550.1481947
CGTGCAA204.1021162E-450.1481948
CAACCGT150.00830727550.1481949
TTAAGAC252.0355175E-550.1481934
CTACCAA150.00830727550.1481946
CCAAAAC204.1254453E-450.0906543
TTAATGT150.00834508950.09064543
GTACTAA150.00834508950.09064543
ACAGGAT204.137149E-450.06192833
CGACCGG150.00836404350.06192433
ATGATCA150.008421149.97595613
AAGAGTG204.172419E-449.97595610
ATAGCCT150.008421149.97595615
GTTAGGA150.008421149.97595632