##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526196_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87509 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.25412243312117 39.0 38.0 40.0 35.0 40.0 2 38.07312390725525 39.0 38.0 40.0 35.0 40.0 3 37.427178918739784 39.0 38.0 39.0 33.0 40.0 4 37.031985281513904 39.0 38.0 39.0 33.0 40.0 5 37.73027917128524 39.0 38.0 40.0 35.0 40.0 6 37.474934006787876 39.0 38.0 39.0 33.0 40.0 7 37.94159457884332 39.0 38.0 40.0 35.0 40.0 8 37.83846232958896 39.0 38.0 40.0 35.0 40.0 9 37.821195534173626 39.0 38.0 40.0 35.0 40.0 10 37.28269092321933 39.0 38.0 39.0 33.0 40.0 11 36.11230844827389 38.0 35.0 39.0 30.0 40.0 12 34.18657509513307 38.0 33.0 39.0 22.0 40.0 13 34.187386440251856 38.0 33.0 39.0 22.0 40.0 14 34.30769406575324 38.0 33.0 39.0 23.0 40.0 15 34.30728267949582 38.0 33.0 39.0 23.0 40.0 16 33.65121301808957 38.0 32.0 39.0 21.0 40.0 17 33.595755865111016 37.0 31.0 39.0 22.0 40.0 18 33.06603892171091 37.0 30.0 39.0 19.0 40.0 19 33.98475585368362 37.0 32.0 39.0 24.0 40.0 20 33.92685323795267 37.0 31.0 39.0 24.0 40.0 21 34.75631077946269 38.0 33.0 39.0 26.0 40.0 22 34.30394587985236 38.0 33.0 39.0 24.0 40.0 23 34.788421762333016 38.0 33.0 39.0 26.0 40.0 24 34.456547326560695 38.0 33.0 39.0 24.0 40.0 25 34.68219268875201 38.0 33.0 39.0 26.0 40.0 26 35.25815630392302 38.0 35.0 39.0 28.0 40.0 27 35.10996583208584 38.0 35.0 39.0 27.0 40.0 28 35.12365585254088 38.0 35.0 39.0 27.0 40.0 29 34.90637534425031 38.0 34.0 39.0 27.0 40.0 30 34.709664148830406 38.0 33.0 39.0 26.0 40.0 31 35.02349472625673 38.0 34.0 39.0 28.0 40.0 32 34.66408026602978 38.0 34.0 39.0 27.0 40.0 33 34.75839056554183 38.0 34.0 39.0 27.0 40.0 34 34.58500268543807 38.0 33.0 39.0 27.0 40.0 35 34.53928167388497 38.0 34.0 39.0 27.0 40.0 36 33.8114365379561 36.0 33.0 38.0 26.0 40.0 37 33.54088150933047 36.0 32.0 38.0 26.0 40.0 38 33.19984230193466 36.0 32.0 38.0 24.0 40.0 39 32.79835216949114 35.0 32.0 38.0 23.0 40.0 40 29.319350009713286 35.0 23.0 38.0 8.0 39.0 41 26.176313293489812 31.0 15.0 37.0 5.0 39.0 42 30.14575643648082 34.0 28.0 38.0 8.0 39.0 43 28.576477847992777 33.0 25.0 38.0 4.0 39.0 44 28.564307671210962 33.0 25.0 38.0 4.0 39.0 45 29.59683004033871 33.0 28.0 38.0 4.0 39.0 46 29.002399753168245 34.0 26.0 38.0 4.0 39.0 47 28.574923722131437 34.0 25.0 38.0 4.0 39.0 48 28.621718908912225 33.0 25.0 38.0 4.0 39.0 49 26.07446091259185 32.0 12.0 38.0 4.0 39.0 50 28.180381446479792 33.0 24.0 38.0 4.0 39.0 51 28.410243517809597 34.0 25.0 38.0 4.0 40.0 52 28.039058839662207 34.0 23.0 38.0 4.0 40.0 53 27.695208492840738 33.0 22.0 38.0 4.0 40.0 54 26.762984378749614 33.0 14.0 38.0 4.0 39.0 55 27.04366408026603 33.0 17.0 38.0 4.0 39.0 56 26.10786319121462 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.37930941443190136 1 2 0.5481382403288961 1 3 1.0622006469562635 1 4 1.6266276868231344 1 5 0.851986351043486 1 6 0.9293165114220585 1 7 0.5930182141061167 1 8 0.7205652282234496 1 9 0.6132378559968288 1 10 0.9469307172668735 1 11 -4.2926579405699705 1 12 -13.901061585420585 1 13 -9.368826915015209 1 14 -6.274997052788564 1 15 -7.374950764422344 1 16 -23.49495792173439 1 17 -4.823128656309471 1 18 -14.948678546308258 1 19 -5.984803831692819 1 20 -5.97650670146912 1 21 -6.431530964771387 1 22 -2.4799621790122757 1 23 -3.397861573370683 1 24 -4.251482088522 1 25 -2.9768464415166953 1 26 2.6299092679162044 1 27 2.1099206934159938 1 28 2.6047265373932333 1 29 2.6279154257426427 1 30 2.6311966603870047 1 31 2.148750081395299 1 32 2.353744167933094 1 33 2.125571498971077 1 34 1.8919394520577697 1 35 2.6092526583305826 1 36 2.4849999470043187 1 37 2.3298120330177525 1 38 2.8802658691786007 1 39 2.507005000722881 1 40 4.164140946074763 1 41 -12.2155425467582 1 42 -11.687252086176024 1 43 -19.12828589150795 1 44 -21.709157155457607 1 45 -13.118837085865342 1 46 -17.581835815999177 1 47 -22.5178859616808 1 48 -16.022139697391303 1 49 -19.23785285820795 1 50 -15.037450008676405 1 51 -12.679254658480703 1 52 -12.119597034221625 1 53 -12.271848985823732 1 54 -11.698379859265472 1 55 -11.835711816073317 1 56 -7.714726685451694 2 1 0.7174046525271365 2 2 0.9112334784241369 2 3 1.297914932670551 2 4 1.8111514963469446 2 5 1.207938731995867 2 6 1.4257450828506322 2 7 0.9311134522013518 2 8 1.1610414186996394 2 9 1.1489521417111135 2 10 1.2552640506002106 2 11 1.015675392763363 2 12 -2.133204442563443 2 13 -2.1200173912056854 2 14 3.1297648519733414 2 15 2.385763521291942 2 16 2.0538516020751345 2 17 3.2149665817857667 2 18 -1.7879642605939736 2 19 1.586624739735754 2 20 2.3056361556737386 2 21 2.4875166542762344 2 22 3.0581330590829623 2 23 2.2533289028197885 2 24 2.0830417210018126 2 25 2.262439272769015 2 26 2.5918140298209664 2 27 2.614682598177893 2 28 2.8487741564408537 2 29 2.843391616218831 2 30 2.9085776127679566 2 31 2.0677977004429167 2 32 2.8001727393616633 2 33 2.2279524513520315 2 34 2.405034690153009 2 35 2.0413955154734396 2 36 2.6933332803376544 2 37 2.8976691758748956 2 38 3.151694440607173 2 39 3.190338334056211 2 40 6.527236184170004 2 41 -5.498875880091532 2 42 -4.658680657604595 2 43 -13.440190653412714 2 44 -13.319871441171893 2 45 -4.237884704912961 2 46 -9.193740577903938 2 47 -16.897647866442703 2 48 -7.087615887867493 2 49 -11.684281429636524 2 50 -6.496973818200214 2 51 -0.46973084895689254 2 52 -0.38745417707876584 2 53 -0.38613470010944795 2 54 0.6170963312107176 2 55 0.2416691363076353 2 56 3.51860664788164 3 1 1.0840713191938036 3 2 1.3031733691345195 3 3 1.761576134856341 3 4 2.497900130226725 3 5 1.5602611363674512 3 6 1.6160456292987178 3 7 1.3245560751521737 3 8 1.3061233859127555 3 9 1.147586021492529 3 10 1.4174225205455642 3 11 2.384391239757896 3 12 4.586604300605956 3 13 3.217824138848961 3 14 3.8756664913176024 3 15 3.4309821005269114 3 16 4.8763925856816925 3 17 4.3340922648458715 3 18 5.117363608258486 3 19 4.486624739735753 3 20 4.072439434362259 3 21 3.374948348265306 3 22 3.4209745891376073 3 23 3.4061977552788107 3 24 4.081538988761373 3 25 3.195909218124207 3 26 2.83662277299036 3 27 3.173152543533085 3 28 3.100276888681293 3 29 2.8451675725029872 3 30 3.403796192002929 3 31 2.8885627277653256 3 32 3.320801154662206 3 33 2.4271327792208837 3 34 2.5961549087322453 3 35 2.9024610892439284 3 36 4.2959289087529555 3 37 4.081958793361238 3 38 3.9948638395142737 3 39 4.491158006187359 3 40 7.829012140454157 3 41 8.25658860078278 3 42 4.300882183925459 3 43 -2.14702125450561 3 44 1.9778061544565233 3 45 3.518399448092506 3 46 2.7693741761944217 3 47 -3.1964183582459853 3 48 2.265526188635242 3 49 4.721319663259653 3 50 2.7890917555702757 3 51 3.681088823174253 3 52 2.9900048393146754 3 53 3.002936338141918 3 54 3.7685990634511555 3 55 2.7068330707338646 3 56 3.5503006369526773 4 1 1.0126427477652342 4 2 0.8592493514400061 4 3 1.3907720755276927 4 4 2.0520245122199583 4 5 0.9853196843768188 4 6 1.4547133368188838 4 7 0.891430912518814 4 8 1.1618350694932928 4 9 0.7005394432984104 4 10 1.0675656379017937 4 11 2.19781824990622 4 12 4.100525716166715 4 13 4.385141338953048 4 14 3.9948442170527088 4 15 3.501239711768129 4 16 4.6971055703291 4 17 4.7832205500397365 4 18 5.056083358453648 4 19 3.756466009577025 4 20 3.9933345683721484 4 21 3.3827547495143264 4 22 3.1994029003528013 4 23 2.967217791708677 4 24 3.435819498779587 4 25 2.6247408600706024 4 26 2.302925140932075 4 27 1.5464286299239305 4 28 1.079329711996408 4 29 1.8247408225680388 4 30 2.497863327053672 4 31 1.5154167480619662 4 32 1.7251727393616605 4 33 1.481127054526631 4 34 1.8252727853910997 4 35 1.7727447218226473 4 36 3.1659523279567026 4 37 3.0202882234939423 4 38 3.045345234257965 4 39 3.5641478578657377 4 40 7.094299676233494 4 41 9.751124119908464 4 42 5.882589183665246 4 43 7.776476013253955 4 44 5.735287288986839 4 45 5.05417878715053 4 46 4.961021326857967 4 47 4.559891816096979 4 48 5.422304747053143 4 49 6.866909046553953 4 50 4.24667697545058 4 51 4.325507246281202 4 52 4.081990267365676 4 53 3.6567224427476965 4 54 4.207969347083733 4 55 3.481748501386999 4 56 3.753527282802274 5 1 1.0168444284375013 5 2 1.2607432823457003 5 3 1.5014163332307717 5 4 2.1229862302404996 5 5 1.3886810289146325 5 6 1.1265153909738856 5 7 0.7168277379156365 5 8 0.7547388976912401 5 9 0.8513330940920625 5 10 1.163737439955952 5 11 1.9359134880014608 5 12 4.413784352954764 5 13 3.9505008160772306 5 14 3.9574959444103186 5 15 4.190315342020234 5 16 4.489355803755807 5 17 4.26314585349445 5 18 4.970649184784179 5 19 4.028641546458445 5 20 4.391560525421639 5 21 2.9779928447524213 5 22 3.422558829391086 5 23 3.04471545744164 5 24 4.029190180385562 5 25 2.994021905822237 5 26 2.189012909372785 5 27 2.5216853992983417 5 28 2.034978638233575 5 29 1.7248341932496416 5 30 2.226154643580287 5 31 1.1638761318154707 5 32 2.4534640558882757 5 33 1.8275322832848033 5 34 1.1894184436544109 5 35 1.0495887927843626 5 36 2.9936834203936797 5 37 3.147739203886104 5 38 3.2825067655371427 5 39 4.1075652248125145 5 40 7.893552710780643 5 41 9.769331402821631 5 42 6.051123263964037 5 43 7.443142679920619 5 44 6.817453488800098 5 45 5.403851989764913 5 46 5.29155353974312 5 47 5.7386966713724235 5 48 4.437244056110099 5 49 8.133015489130987 5 50 4.490841307850207 5 51 3.9319498233120136 5 52 4.3812433019128285 5 53 3.893417120618846 5 54 4.820947871826963 5 55 3.2679296405093154 5 56 3.9939567879376625 6 1 1.057984362672066 6 2 1.2239146378444232 6 3 1.324726526873448 6 4 2.259339902144042 6 5 1.2387358334451406 6 6 1.3384262422709199 6 7 0.8050264956796127 6 8 1.1284327230474673 6 9 1.1917057648995169 6 10 1.329539412919047 6 11 2.6986464072561205 6 12 4.9055636733785875 6 13 3.9230985508232976 6 14 4.734112678060299 6 15 4.922357724190491 6 16 5.124503775988174 6 17 5.272937596278517 6 18 5.563122695927767 6 19 4.456189957127059 6 20 4.228462242630258 6 21 2.807806509348694 6 22 2.8566837837206407 6 23 3.260937598471962 6 24 3.0913750543351455 6 25 2.360627678566118 6 26 2.2642777979369058 6 27 2.463957960496735 6 28 2.620875605716215 6 29 2.4995510365086844 6 30 2.751693554796944 6 31 2.9909861062400225 6 32 2.8932886813906507 6 33 2.390995929612899 6 34 1.9590201973993828 6 35 2.3533520372125665 6 36 3.2560144397579407 6 37 2.9306401903676473 6 38 3.0285060348100714 6 39 3.9794687688388244 6 40 6.9529608218511605 6 41 4.088805279328756 6 42 5.1963918061635255 6 43 6.564881810355402 6 44 6.1210705878136125 6 45 4.925883411029066 6 46 5.317128987313453 6 47 5.733873872687731 6 48 5.088833387494827 6 49 8.08347219355188 6 50 5.8921566165823975 6 51 5.356356107564846 6 52 6.894067562051667 6 53 6.155894285397796 6 54 6.1587629978773855 6 55 4.629712614568504 6 56 4.178026937736714 7 1 1.0840713191938036 7 2 1.0741122663029259 7 3 1.6994300841856997 7 4 1.911909072104521 7 5 1.1814235804807183 7 6 1.6522602343657837 7 7 1.0220225431104453 7 8 1.138314145972366 7 9 0.6474369901959562 7 10 1.2196579899941469 7 11 2.3346147867027582 7 12 4.556947072588073 7 13 4.006497760309465 7 14 4.375219397427884 7 15 4.380460490988909 7 16 5.065215238438768 7 17 4.863451430270615 7 18 5.4915811939514825 7 19 4.377533830644843 7 20 4.489727064764644 7 21 3.484486351245934 7 22 4.146011846961748 7 23 3.0692379937288834 7 24 3.518647781607868 7 25 3.531378666708413 7 26 2.625147363154298 7 27 2.6358947193900164 7 28 2.535895368562066 7 29 2.497179495006712 7 30 2.8896382188285656 7 31 1.9435552762004988 7 32 2.758506072694999 7 33 2.068861542261118 7 34 2.2633680234863434 7 35 2.893668242746166 7 36 3.4243938863982635 7 37 2.5484267516324692 7 38 3.775936864849591 7 39 3.413065606783487 7 40 6.608296790230607 7 41 -0.488269819485474 7 42 4.37465267572874 7 43 5.624960861738799 7 44 6.256643710343262 7 45 4.322721355693101 7 46 5.092623058459701 7 47 5.659170315375476 7 48 5.89950532425372 7 49 7.6573852370301445 7 50 6.025753454527056 7 51 5.902996423770379 7 52 5.655727641103049 7 53 6.447198633223888 7 54 7.531490270604657 7 55 6.362123681762178 7 56 5.724667253942247 8 1 1.2255807531560663 8 2 1.451642283455584 8 3 1.9114369452491644 8 4 2.523572879994745 8 5 1.7562091722474378 8 6 1.8737010576934026 8 7 1.3367738295598457 8 8 1.2280225507751155 8 9 1.263732015924944 8 10 1.7224024153800812 8 11 2.8563986632036134 8 12 5.407204362468008 8 13 4.7902027345804825 8 14 4.667500701029944 8 15 5.17239874141773 8 16 5.238285564339286 8 17 5.274086078641112 8 18 5.76722441865131 8 19 4.901719079358394 8 20 4.514598419824679 8 21 3.7340575347793816 8 22 4.3895795999634615 8 23 3.634932676404695 8 24 3.840431658108727 8 25 3.873917260190403 8 26 3.0345813254184506 8 27 3.3700285101275824 8 28 3.5961012004660162 8 29 3.0554985344578256 8 30 3.588609059308837 8 31 2.842011536920907 8 32 3.275487204770471 8 33 2.5949335834275047 8 34 2.2426133065052056 8 35 2.9248860815111755 8 36 2.5648741608408017 8 37 2.7997132010321266 8 38 2.518990038091445 8 39 3.445998711414706 8 40 5.7751921590127715 8 41 -2.132523678833671 8 42 3.8274828644079832 8 43 4.895972868599863 8 44 5.046009061972761 8 45 2.480354288797731 8 46 4.093995271152664 8 47 4.386786095821449 8 48 4.067258325968986 8 49 6.453611652124483 8 50 3.3115167478375227 8 51 4.692533301986504 8 52 4.175281671977835 8 53 4.8660665577521875 8 54 5.405932809198138 8 55 4.541197438194427 8 56 4.593449415177236 9 1 1.0840713191938036 9 2 1.206359264587654 9 3 1.552946379211427 9 4 2.080176653579649 9 5 1.1996053986625341 9 6 1.411436906750005 9 7 1.0726228861636145 9 8 1.1619848149260577 9 9 0.8863735253589127 9 10 0.9865533587763125 9 11 2.318662814147011 9 12 4.152487381335931 9 13 4.2052970742031235 9 14 4.337312021784662 9 15 4.247870439530935 9 16 4.511870469999657 9 17 5.028803059773189 9 18 4.984205550726784 9 19 3.7979454944527333 9 20 4.373088985862417 9 21 3.3944348932699455 9 22 3.7952399773219554 9 23 3.540593053763189 9 24 3.4536392052785416 9 25 3.1758040526432296 9 26 2.657222834852412 9 27 2.9455002082407873 9 28 2.9734596910320477 9 29 2.7819136287974473 9 30 3.2112505687427984 9 31 2.502388895411471 9 32 2.473600412317637 9 33 1.5288958475784469 9 34 2.280349155561815 9 35 2.472055892831932 9 36 2.7158175570672114 9 37 3.5449962199000566 9 38 3.8680466418650283 9 39 3.3988289000939496 9 40 6.331795932597675 9 41 4.367476321166329 9 42 4.5067281474268555 9 43 6.603520038411183 9 44 6.140348684614274 9 45 5.027524100118487 9 46 6.697768856058325 9 47 5.839616284500693 9 48 5.991786627855777 9 49 9.189460708728259 9 50 6.245479011988465 9 51 5.758571037835562 9 52 5.713017521034438 9 53 5.884934482280489 9 54 7.122913941273609 9 55 6.248744608005751 9 56 5.442506018950823 10 1 1.1994559345784168 10 2 1.4349514271420816 10 3 1.763940573696189 10 4 2.2028181630136103 10 5 1.3246053986625341 10 6 1.3994630315685797 10 7 1.2868826829705853 10 8 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-4.005282083204559 115 56 -1.49927953098015 116 1 0.2968372766406091 116 2 0.4365880883532114 116 3 1.0493333723868616 116 4 1.6283500779072284 116 5 0.5825841220667911 116 6 0.6085465012903484 116 7 0.03749643092475452 116 8 0.3089137591251756 116 9 0.17200178710118053 116 10 0.6469307172668763 116 11 -2.418189855463588 116 12 -13.363168981570535 116 13 -8.905301079148948 116 14 -3.4021500416436794 116 15 -5.3665414432470655 116 16 -3.6009356319674204 116 17 -2.5609199430369287 116 18 -7.584240856338653 116 19 -2.960183770902546 116 20 -2.749151078368815 116 21 1.993190413141484 116 22 2.5106153285865105 116 23 1.7804565623942565 116 24 2.4594601607181232 116 25 2.0338931734782406 116 26 2.2166367248564214 116 27 2.181349264844563 116 28 1.3500152911926264 116 29 1.707044098488339 116 30 2.0849960524842714 116 31 0.9323367075351214 116 32 1.2606337322694614 116 33 1.2290162811392662 116 34 1.0633680234863405 116 35 1.289799770792584 116 36 2.4197517200539664 116 37 2.0435911616905003 116 38 2.159495859046885 116 39 2.987146844694511 116 40 6.2866333472905715 116 41 4.7333936234545675 116 42 0.342737782111719 116 43 -8.216431788164488 116 44 -6.395580661030049 116 45 0.17204437310122245 116 46 -4.28416610981883 116 47 -2.1120095685703646 116 48 -3.673608795668912 116 49 -7.974529656586878 116 50 -3.4618674352214924 116 51 -2.39739042342498 116 52 -1.9581988579298297 116 53 -3.0996098774144123 116 54 -7.981662534037508 116 55 -2.393969161564705 116 56 -1.2608259762318355 117 1 -0.09503315841813986 117 2 0.11061158787687475 117 3 0.8872184152576139 117 4 1.2550569689837587 117 5 0.17721733896104297 117 6 0.8400485654376979 117 7 0.13260598951478642 117 8 0.2423847022817327 117 9 0.1626337337509156 117 10 0.4006620605504594 117 11 -2.77624003012221 117 12 -7.165169616692793 117 13 -12.24302734145444 117 14 -12.295608282355015 117 15 -9.11709717522547 117 16 -15.427864815835317 117 17 -12.697471229159508 117 18 -12.122665753131287 117 19 -6.1477036184732015 117 20 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4.653879376658264 118 41 2.316851819438984 118 42 -3.702812113003656 118 43 -10.55685732007938 118 44 -10.351678952908983 118 45 -4.2524386955232885 118 46 -12.338106775087036 118 47 -14.059267584752565 118 48 -11.940315418383923 118 49 -13.67500912916704 118 50 -9.948382268904439 118 51 -7.734519581351261 118 52 -7.608933050318203 118 53 -7.103505592128226 118 54 -13.035603199305713 118 55 -6.3481195960867325 118 56 -4.5691867793484064 119 1 -0.3646466295241453 119 2 -0.052228060037400326 119 3 0.35047903523464896 119 4 1.1002540604495081 119 5 0.14832334738048303 119 6 0.5597194418249956 119 7 -0.2996557785678817 119 8 -0.20690729924907458 119 9 0.03997778273674868 119 10 0.6469307172668763 119 11 -6.0695810174930465 119 12 -11.712050596409593 119 13 -11.819376365564658 119 14 -11.729209507001016 119 15 -9.278980068451649 119 16 -13.848071474847945 119 17 -16.479905213086028 119 18 -17.6911693887991 119 19 -7.410811157700145 119 20 -8.739876664839084 119 21 -0.5201756534160751 119 22 0.14018434113424405 119 23 0.07832890281979132 119 24 0.45291351587360396 119 25 0.2682085035382471 119 26 1.4097627477696832 119 27 1.3095543930496945 119 28 0.9468510795177778 119 29 1.1696736675008808 119 30 1.463064792255139 119 31 -0.09630486365964686 119 32 -5.159442645253723 119 33 0.17282424622382564 119 34 0.10182956194788062 119 35 -0.19001474093681736 119 36 1.9388461008504763 119 37 2.229079432285154 119 38 1.6875918765046052 119 39 2.2839280776459567 119 40 5.884287466221284 119 41 -0.043747674963327654 119 42 -4.1638088627328 119 43 -10.608139371361432 119 44 -10.879486825787279 119 45 -6.4891667561950115 119 46 -16.328355962519325 119 47 -12.981622225417064 119 48 -14.212615887867491 119 49 -12.45543527579037 119 50 -12.521332792559189 119 51 -10.667166746392791 119 52 -9.900915715540307 119 53 -10.165621879596628 119 54 -12.218160079045692 119 55 -9.267946248307748 119 56 -7.650624121349129 120 1 -1.0913672772974223 120 2 -0.7506086268390249 120 3 -0.6906815585575217 120 4 0.018607636697822727 120 5 -1.0196928469515 120 6 -0.8431145662721704 120 7 -1.2706409337635591 120 8 -1.0152743707740441 120 9 -1.2063110161836264 120 10 -1.7127184055401443 120 11 -4.9487982914471615 120 12 -13.066976372388002 120 13 -13.00291212804779 120 14 -16.229884270833676 120 15 -14.145376829585253 120 16 -17.92904313476697 120 17 -14.167489558565112 120 18 -16.764718646558883 120 19 -9.048462979562494 120 20 -14.305328756606965 120 21 -5.387921942214994 120 22 -4.273445888285458 120 23 -3.746232500688979 120 24 -9.140203893033277 120 25 -3.6204554640730855 120 26 0.033919292978858095 120 27 -0.38882617375193007 120 28 -0.046401282155635215 120 29 0.02190038814865858 120 30 0.38006884083813475 120 31 -1.4181672118377833 120 32 -14.380090418533072 120 33 -1.595731759174285 120 34 -1.5331232045838377 120 35 -2.1441308003160344 120 36 -1.1185088249255024 120 37 -0.5273308241251016 120 38 0.023624265168571412 120 39 -0.4859774554174727 120 40 3.689955482415616 120 41 -2.327823248512587 120 42 -6.5288560962010855 120 43 -13.92527837271096 120 44 -9.643994248189436 120 45 -9.481744354035769 120 46 -16.82093356036008 120 47 -17.184051375214636 120 48 -12.967001852779772 120 49 -17.504018271741785 120 50 -13.88907908135811 120 51 -11.287274708606017 120 52 -10.87999803672789 120 53 -10.986134700109448 120 54 -13.465798405631388 120 55 -11.181576477727452 120 56 -8.749814404749937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 169.0 5 36.0 6 72.0 7 78.0 8 182.0 9 215.0 10 128.0 11 120.0 12 123.0 13 120.0 14 163.0 15 202.0 16 284.0 17 394.0 18 446.0 19 537.0 20 696.0 21 889.0 22 1060.0 23 1382.0 24 1530.0 25 1769.0 26 1936.0 27 2186.0 28 2704.0 29 3092.0 30 3466.0 31 4299.0 32 4758.0 33 5355.0 34 6387.0 35 7597.0 36 9545.0 37 11166.0 38 9431.0 39 4988.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.45719868813494 0.4650950187980665 0.04113862574135232 0.03656766732564651 8 99.61832497228856 0.26625832771486363 0.0537087613845433 0.06170793861202848 9 94.93994903381366 3.3036601949513766 0.9621867465060737 0.794204024728885 10 47.872790227290906 36.70822429692946 7.960324080951674 7.458661394827961 11 39.62335302654584 25.4179570101361 16.26347004308128 18.695219920236774 12 35.949445199922295 13.802008936223704 22.88907426664686 27.359471597207147 13 24.6999657103669 16.433878157503713 25.614355926391585 33.251800205737794 14 23.739486194916804 18.89742183214482 29.778067288352535 27.585024684585846 15 28.54803055813261 17.63621161621368 27.21139859579196 26.604359229861757 16 31.99135585080839 20.457819753481672 24.18646664684763 23.36435774886231 17 29.011873064484796 23.086768218126135 23.23989532505228 24.66146339233679 18 28.652237509713395 22.48594414224985 22.98875531379988 25.87306303423687 19 27.39243344659139 26.497809200215077 22.516616901762937 23.593140451430596 20 29.86479685212299 23.39632137628111 22.530426427525622 24.20845534407028 21 28.72390268429533 22.69252305477151 24.623752985407215 23.959821275525947 22 27.454318984333042 20.42075672216572 28.38793724074095 23.736987052760288 23 29.248420162497574 20.649304642951012 26.03617913586031 24.066096058691105 24 30.50314824760882 20.940703241952257 24.48662423293604 24.069524277502886 25 26.70674010924965 21.534523346970495 25.657669188398508 26.101067355381346 26 24.33025509737588 19.476318917436224 30.58311237085124 25.610313614336654 27 23.52799862723789 17.683463936395356 30.87685179889035 27.911685637476406 28 23.333333333333332 21.061906396612883 30.62478544455887 24.979974825494907 29 26.43202523976635 18.960688606668878 32.0218104502692 22.585475703295575 30 23.048348382672305 17.501428734712537 36.30586352726026 23.144359355354897 31 25.349504463826435 17.26203404167762 36.05582926578343 21.33263222871252 32 27.75299661451185 15.918656784701254 37.19347607283375 19.13487052795315 33 24.90269255426321 16.723143144976646 40.69855298104222 17.675611319717923 34 20.568024605252628 17.908553526714766 44.857708006997406 16.665713861035204 35 20.274627275221803 22.063934876063296 41.92124759901216 15.740190249702735 36 20.94926724433572 23.20583460984476 38.94579208486705 16.89910606095247 37 23.394143447738802 21.219408847814385 38.02521730168001 17.361230402766804 38 22.75389393817836 21.35792139918167 38.95886580096503 16.92931886167494 39 24.060907893983625 18.886026675825747 38.943270937088556 18.109794493102065 40 16.646712101676183 13.932123779701602 34.58970344446491 34.831460674157306 41 13.595950044397293 14.903652109736267 33.528604541208765 37.97179330465768 42 18.844045725532375 18.311954320238925 39.95491526587406 22.889084688354636 43 17.220484977606727 14.604309130270437 40.53468952956943 27.64051636255341 44 18.101438791093962 15.182518630794492 40.15754360581947 26.558498972292078 45 21.062516462248485 18.23387272248371 40.814924245026965 19.888686570240836 46 19.74973668544214 19.592892796629574 40.31002427073316 20.347346247195127 47 18.840099230619735 17.116138694225647 41.72030226469882 22.323459810455798 48 20.737527611504696 18.744921199913016 42.849621736692114 17.66792945189017 49 17.94005812684518 15.324350093623279 36.62910248015531 30.10648929937623 50 18.874725375320395 19.34845294763823 41.42141157085317 20.35541010618821 51 18.997936253152947 19.058702132538407 41.83444164182527 20.108919972483374 52 19.082869009072915 18.847179158610118 40.47229500131574 21.597656831001224 53 18.33894192248701 19.454936703065265 42.729437080786575 19.47668429366115 54 16.925720176393106 18.976003665311264 39.27151938606036 24.826756772235267 55 18.562428407789234 20.153493699885452 39.61970217640321 21.66437571592211 56 19.9340568759445 21.227732747172233 38.42675275907863 20.411457617804643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 3.5 13 14.5 14 22.0 15 50.5 16 79.0 17 140.0 18 201.0 19 258.5 20 316.0 21 481.0 22 715.5 23 785.0 24 914.0 25 1043.0 26 1174.5 27 1306.0 28 1576.0 29 2225.5 30 2605.0 31 2889.0 32 3173.0 33 3401.0 34 3629.0 35 3594.0 36 3559.0 37 3168.0 38 2402.5 39 2028.0 40 1781.0 41 1534.0 42 1459.0 43 1384.0 44 1406.0 45 1428.0 46 1416.0 47 1421.0 48 1438.0 49 1534.0 50 1630.0 51 1777.5 52 1925.0 53 2015.0 54 2318.5 55 2532.0 56 2868.0 57 3204.0 58 3562.5 59 3921.0 60 4272.0 61 4623.0 62 5168.0 63 5943.5 64 6174.0 65 6225.5 66 6277.0 67 5657.5 68 5038.0 69 4520.5 70 4003.0 71 3462.5 72 2568.0 73 2214.0 74 1853.0 75 1492.0 76 1270.0 77 1048.0 78 884.0 79 576.5 80 433.0 81 311.0 82 189.0 83 135.5 84 82.0 85 58.5 86 35.0 87 25.5 88 9.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.021712052474602613 14 0.005713698019632267 15 0.22854792078529068 16 0.05827971980024912 17 0.0 18 0.0011427396039264534 19 0.11198848118479242 20 0.09713286633374853 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00342821881177936 26 0.014855614851043893 27 0.1074175227690866 28 0.13598601286724793 29 0.03199670890994069 30 0.021712052474602613 31 0.03199670890994069 32 0.0879909495023369 33 0.17941011781645316 34 0.05485150098846976 35 0.0514232821766904 36 0.035424927721720054 37 0.2148350455381732 38 0.29482681781302494 39 0.18740929504393833 40 0.7370670445325623 41 0.905049766309751 42 0.1348432732633215 43 0.23540435840884938 44 0.48223611285696333 45 0.2148350455381732 46 0.18398107623215898 47 0.04113862574135232 48 0.15541258613399764 49 0.5233747385983156 50 0.13370053365939502 51 0.330251745534745 52 0.12113039801620404 53 0.16341176336148283 54 0.23311887920099647 55 0.23883257722062873 56 0.18398107623215898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 87509.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.59585871167536 #Duplication Level Percentage of deduplicated Percentage of total 1 86.16989891775228 63.417477059502446 2 7.934412993183547 11.678798752128353 3 2.6039159666475165 5.749122947353986 4 1.1583311336428428 3.4099349781165365 5 0.6086672981072311 2.2397696236958486 6 0.36488983432448796 1.611262841536299 7 0.28259553126407155 1.4558502554023014 8 0.21738117789543965 1.2798683563976276 9 0.12887598403801065 0.8536264841330606 >10 0.5015294318587643 6.067947296849467 >50 0.021738117789543963 1.038750299969146 >100 0.007763613496265702 1.197591104914923 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 268 0.30625421385228946 No Hit CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 258 0.29482681781302494 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 222 0.25368819207167265 No Hit CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 176 0.2011221702910558 No Hit CCGACGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCCAGGCTCACAACCAAA 124 0.14169971088688021 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0034282188117793597 0.0 0.0 0.0 0.0 11 0.005713698019632266 0.0 0.0 0.0 0.0 12 0.009141916831411627 0.0 0.0 0.0 0.0 13 0.01028465643533808 0.0 0.0 0.0 0.0 14 0.014855614851043893 0.0 0.0 0.0 0.0 15 0.0171410940588968 0.0 0.0 0.0 0.0 16 0.0171410940588968 0.0 0.0 0.0 0.0 17 0.019426573266749705 0.0 0.0 0.0 0.0 18 0.019426573266749705 0.0 0.0 0.0 0.0 19 0.023997531682455518 0.0 0.0 0.0 0.0 20 0.023997531682455518 0.0 0.0 0.0 0.0 21 0.025140271286381972 0.0 0.0 0.0 0.0 22 0.026283010890308427 0.0 0.0 0.0 0.0 23 0.027425750494234877 0.0 0.0 0.0 0.0 24 0.033139448513867145 0.0 0.0 0.0 0.0 25 0.03885314653349941 0.0 0.0 0.0 0.0 26 0.04228136534527877 0.0 0.0 0.0 0.0 27 0.047995063364911036 0.0 0.0 0.0 0.0 28 0.050280542572763945 0.0 0.0 0.0 0.0 29 0.05256602178061685 0.0 0.0 0.0 0.0 30 0.0537087613845433 0.0 0.0 0.0 0.0 31 0.05599424059239621 0.0 0.0 0.0 0.0 32 0.05713698019632266 0.0 0.0 0.0 0.0 33 0.05827971980024912 0.0 0.0 0.0 0.0 34 0.05942245940417557 0.0 0.0 0.0 0.0 35 0.06170793861202848 0.0 0.0 0.0 0.0 36 0.06399341781988138 0.0 0.0 0.0 0.0 37 0.06513615742380784 0.0 0.0 0.0 0.0 38 0.06742163663166074 0.0 0.0 0.0 0.0 39 0.0685643762355872 0.0 0.0 0.0 0.0 40 0.06970711583951365 0.0 0.0 0.0 0.0 41 0.07084985544344011 0.0 0.0 0.0 0.0 42 0.07313533465129302 0.0 0.0 0.0 0.0 43 0.07542081385914591 0.0 0.0 0.0 0.0 44 0.07770629306699882 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGAC 55 0.0 50.263676 41 GTTAAAC 20 4.055773E-4 50.26367 40 ATAACCT 15 0.008232031 50.26367 40 GAGTACG 15 0.008269589 50.205864 36 AAGGCCA 25 2.021706E-5 50.205864 36 AAAGGCC 25 2.028602E-5 50.177013 35 TACCAAG 15 0.008307275 50.14819 47 CGTGCAA 20 4.1021162E-4 50.14819 48 CAACCGT 15 0.008307275 50.14819 49 TTAAGAC 25 2.0355175E-5 50.14819 34 CTACCAA 15 0.008307275 50.14819 46 CCAAAAC 20 4.1254453E-4 50.09065 43 TTAATGT 15 0.008345089 50.090645 43 GTACTAA 15 0.008345089 50.090645 43 ACAGGAT 20 4.137149E-4 50.061928 33 CGACCGG 15 0.008364043 50.061924 33 ATGATCA 15 0.0084211 49.975956 13 AAGAGTG 20 4.172419E-4 49.975956 10 ATAGCCT 15 0.0084211 49.975956 15 GTTAGGA 15 0.0084211 49.975956 32 >>END_MODULE