FastQCFastQC Report
Fri 17 Jun 2016
SRR1526195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75738
Sequences flagged as poor quality0
Sequence length56
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7981.053632258575616No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1970.26010721170350415No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1780.2350207293564657No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1700.22445799994718635No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1040.13731548232063165No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1010.13335445879215188No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA880.11619002350207294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAG150.0082682450.201244
GCGCCCG150.0082682450.201244
CGATTCA204.090596E-450.16777849
CGATTAA204.090596E-450.16777849
CCACTAT150.00829005450.16777442
AACGATT355.0924427E-850.13439647
TGATATG204.1040682E-450.13439650
ATTTGAT204.1040682E-450.13439647
CTTCGGG150.00831191250.13439647
ACGATTC204.1040682E-450.13439648
ACGATTA204.1040682E-450.13439648
CAACGAT355.0924427E-850.13439646
ATTCGTG204.117576E-450.10106714
TTCGTGG252.0441803E-550.10106315
AACGAAG150.008333812550.10106314
CAAGGAC150.008333812550.10106333
GTAGTGG150.008333812550.10106314
CCAGTGA150.00839977150.00132813
AATTCGT204.1583105E-450.00132813
GTGACAG150.00842184349.96816616