FastQCFastQC Report
Fri 17 Jun 2016
SRR1526194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106505
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG35013.2871696164499324No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG9720.9126332097084643No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG9240.8675649030561946No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG7640.7173372142152951No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.4018590676494061No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA4180.3924698370968499No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2760.25914276325055163No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA2680.25163137880850667No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.25163137880850667No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA2470.23191399464813858No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA1790.16806722689075632No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1780.16712830383550067No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1770.16618938078024506No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1680.15773907328294445No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1450.13614384301206514No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1390.13051030468053143No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1370.12863245857002018No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10891507440965213No Hit
TAGTCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1140.10703722829914089No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA1080.10140368996760715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGAC150.0080227650.5995238
ACACCCG150.00803791850.57538240
CCACTGA150.00803791850.57538240
TTCCACC150.00803791850.57538239
TCGTCTC950.050.57538240
CTTGACT251.9386393E-550.57538239
TAGTCTC203.9376804E-450.5753840
CACCGAA150.00805309850.55126641
TATGCCT1000.050.38308335
TATGCCC204.0124683E-450.3830835
ATGCCTG900.050.38307636
ATATGCA150.00823692150.26363434
TGTATAT150.00831442850.1447548
TCCACGA355.129732E-850.1210445
CAAGGAG150.00842384849.97925633
AAGGTCC150.00845530649.9321727
TGTTCAC150.00845530649.9321714
ATGGTTG150.00848685249.88517413
AGTATGG150.008502657549.86171319
ATCAAAG150.008502657549.86171317
Produced by FastQC (version 0.11.5)