##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526194_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 106505 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.08358293037885 39.0 38.0 40.0 35.0 40.0 2 36.74917609501901 39.0 38.0 39.0 32.0 40.0 3 37.34809633350547 39.0 38.0 39.0 33.0 40.0 4 37.508295385193186 39.0 38.0 40.0 33.0 40.0 5 37.71243603586686 39.0 38.0 40.0 35.0 40.0 6 37.56674334538285 39.0 38.0 39.0 35.0 40.0 7 37.837810431435145 39.0 38.0 40.0 35.0 40.0 8 37.74696023660861 39.0 38.0 40.0 35.0 40.0 9 37.65413830336604 39.0 38.0 40.0 35.0 40.0 10 37.19268578939956 39.0 38.0 39.0 33.0 40.0 11 35.96495000234731 38.0 35.0 39.0 30.0 40.0 12 34.391408854044414 38.0 33.0 39.0 23.0 40.0 13 34.27441904135956 38.0 33.0 39.0 23.0 40.0 14 34.23028965776255 38.0 33.0 39.0 22.0 40.0 15 34.12951504624196 38.0 33.0 39.0 22.0 40.0 16 33.60832824750012 38.0 32.0 39.0 20.0 40.0 17 33.560123937843294 38.0 31.0 39.0 20.0 40.0 18 33.12316792638843 37.0 31.0 39.0 18.0 40.0 19 33.96041500399042 38.0 32.0 39.0 23.0 40.0 20 33.930500915449976 37.0 32.0 39.0 23.0 40.0 21 34.74201211210741 38.0 33.0 39.0 25.0 40.0 22 34.46803436458382 38.0 33.0 39.0 24.0 40.0 23 35.03024271160978 38.0 34.0 39.0 26.0 40.0 24 34.59316464015774 38.0 33.0 39.0 24.0 40.0 25 34.82122905027933 38.0 33.0 39.0 26.0 40.0 26 35.411332801276934 38.0 35.0 39.0 28.0 40.0 27 35.402694709168586 38.0 35.0 39.0 28.0 40.0 28 35.179390638937136 38.0 35.0 39.0 27.0 40.0 29 35.022872165626026 38.0 35.0 39.0 27.0 40.0 30 34.71304633585277 38.0 34.0 39.0 26.0 40.0 31 35.00499507065396 38.0 35.0 39.0 27.0 40.0 32 34.75080043190461 38.0 35.0 39.0 26.0 40.0 33 34.93338340922961 38.0 35.0 39.0 27.0 40.0 34 34.81514482888127 38.0 35.0 39.0 27.0 40.0 35 34.659161541711654 38.0 35.0 39.0 26.0 40.0 36 34.03584808224966 38.0 33.0 39.0 26.0 40.0 37 33.76655556077179 37.0 33.0 39.0 25.0 40.0 38 33.541767992113044 37.0 33.0 39.0 23.0 40.0 39 33.02004600722971 36.0 32.0 39.0 20.0 40.0 40 29.817708088822123 35.0 24.0 38.0 8.0 40.0 41 26.633481996150415 32.0 15.0 38.0 5.0 39.0 42 30.588695366414722 35.0 29.0 38.0 9.0 39.0 43 29.202741655321347 34.0 26.0 38.0 4.0 39.0 44 29.3056006760246 34.0 26.0 38.0 4.0 39.0 45 30.27062579221633 35.0 28.0 38.0 4.0 39.0 46 29.9289329139477 35.0 27.0 38.0 4.0 40.0 47 29.49924416694052 35.0 27.0 38.0 4.0 40.0 48 29.501056288437162 35.0 27.0 38.0 4.0 40.0 49 27.249631472700813 33.0 17.0 38.0 4.0 39.0 50 29.108774235951365 35.0 26.0 38.0 4.0 40.0 51 29.27125487066335 35.0 27.0 39.0 4.0 40.0 52 29.078766255105393 35.0 26.0 39.0 4.0 40.0 53 28.592929909393924 35.0 23.0 39.0 4.0 40.0 54 27.707497300596216 35.0 18.0 39.0 4.0 40.0 55 27.931176940049763 35.0 21.0 38.0 4.0 40.0 56 26.845969672785316 33.0 17.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.2003765034592888 1 2 1.8680534659470425 1 3 0.7202974471817001 1 4 0.3776402365057905 1 5 0.21755953710920295 1 6 0.5370178839323572 1 7 0.521377155169894 1 8 0.5746638982587484 1 9 0.5132334207420968 1 10 0.6785554157980656 1 11 -2.885966608133238 1 12 -14.527670862856706 1 13 -10.257075030771809 1 14 -5.369875358385961 1 15 -6.280907804612934 1 16 -23.17772563345461 1 17 -5.299956589756327 1 18 -13.937552793975428 1 19 -5.805905519108716 1 20 -5.400386303679227 1 21 -5.523020449561415 1 22 -2.587527053721857 1 23 -2.8914410309435006 1 24 -3.471098733735616 1 25 -3.7543950275826177 1 26 2.386757173641307 1 27 1.8125922938012806 1 28 1.943275323550047 1 29 2.413230011865487 1 30 2.383515162903933 1 31 2.4320484159500566 1 32 2.1468345692050974 1 33 1.6079460956163345 1 34 1.8951034543853638 1 35 1.607825550658923 1 36 2.400287194226756 1 37 1.9622175177451453 1 38 2.3345574348555402 1 39 3.0339514724982664 1 40 5.480581068325751 1 41 -10.007695248228439 1 42 -9.990285695802925 1 43 -17.262927957549138 1 44 -22.199330331842063 1 45 -10.890071810245196 1 46 -17.1264800925406 1 47 -22.44533104182266 1 48 -13.987317025434605 1 49 -19.763312500952928 1 50 -13.613973713374552 1 51 -10.153080270087766 1 52 -10.092306240271512 1 53 -9.85437884918457 1 54 -9.085711336403673 1 55 -9.21484450978571 1 56 -4.876645456297126 2 1 0.9103742077751775 2 2 2.5975070931279447 2 3 1.266498089973247 2 4 1.12649239444886 2 5 1.2610627510669588 2 6 1.212867287054486 2 7 1.1063748594857827 2 8 1.2469549910043867 2 9 1.060811474001973 2 10 1.2795655168081694 2 11 1.0840747141808151 2 12 -2.564688769192795 2 13 -1.573707262176768 2 14 2.9651223459299274 2 15 2.957498990612045 2 16 2.2182798761872604 2 17 3.2900916196100667 2 18 -1.4067332347467776 2 19 2.0797694120207595 2 20 2.41131020688092 2 21 2.3716076496121374 2 22 2.605253019740033 2 23 1.6477003831979147 2 24 1.061729549092668 2 25 1.8164842194329935 2 26 2.0418880276357996 2 27 2.137202027501921 2 28 2.417965406194675 2 29 2.5232506730225097 2 30 2.6941786156128416 2 31 2.154901951303593 2 32 2.0970182239342066 2 33 2.1863437081048573 2 34 2.208349551722371 2 35 1.7631859730648003 2 36 2.5533519325187726 2 37 2.2886637987368843 2 38 2.6517635872889684 2 39 2.958997386180542 2 40 5.177034236369831 2 41 -6.6938752298629645 2 42 -7.342558423075651 2 43 -13.765567994280083 2 44 -15.365193509068877 2 45 -7.976504317132246 2 46 -12.022313425873932 2 47 -17.91278971950861 2 48 -9.625287640677946 2 49 -14.212176137316565 2 50 -9.007913107313946 2 51 -4.721778617195206 2 52 -4.32508860941752 2 53 -3.7783917050156077 2 54 -3.21346615734031 2 55 -3.001632847710411 2 56 -0.4322584039555295 3 1 0.8183253636290146 3 2 2.302298293724512 3 3 1.1907616992200758 3 4 1.258110589825442 3 5 1.1940418711266148 3 6 0.9563423056972908 3 7 0.9446951428564034 3 8 1.0697551401468175 3 9 0.8586768729579717 3 10 1.2844126458163672 3 11 2.3063901504224233 3 12 3.729914139084613 3 13 2.3260410599708834 3 14 2.946060078964976 3 15 2.555466924989375 3 16 4.187519249789055 3 17 4.843922715806933 3 18 4.877522917378499 3 19 3.87954569837423 3 20 3.405204688610972 3 21 3.2961042939074403 3 22 3.4388193881218356 3 23 2.751634014816112 3 24 3.1756370807854353 3 25 3.128238507277885 3 26 2.163672143966892 3 27 2.246448858225264 3 28 2.6616734598859466 3 29 2.1738379213446564 3 30 2.3936100100945765 3 31 1.8182595202819698 3 32 2.429046560996099 3 33 2.030210225629091 3 34 1.867093026741017 3 35 1.5898545040118535 3 36 2.4385588676418095 3 37 2.4933617853140646 3 38 3.103152476177854 3 39 3.272289705345351 3 40 6.412912312432471 3 41 7.3294282749841635 3 42 4.107944932629046 3 43 -1.46737634625622 3 44 0.6595082060690807 3 45 3.220084049758132 3 46 1.5245266188687943 3 47 -4.071766229575722 3 48 1.7928704502989348 3 49 3.392270171408267 3 50 2.1956663110305747 3 51 3.724949570724256 3 52 3.693927050537738 3 53 3.7363361100477768 3 54 4.654263149147386 3 55 3.892149777196373 3 56 5.303619672107111 4 1 0.9781294630304345 4 2 2.642176762797618 4 3 1.5906347266098848 4 4 1.43655245450892 4 5 1.4003270153312215 4 6 1.471125545312745 4 7 1.1910220066329273 4 8 1.410994030043426 4 9 1.3534165791070762 4 10 1.4402261774688299 4 11 2.77664228174838 4 12 4.205018438014413 4 13 3.975279224309716 4 14 4.509228952536532 4 15 4.06072721884027 4 16 5.024586182493572 4 17 4.8604745024929485 4 18 5.201937936424393 4 19 4.656345988597337 4 20 3.968554951625663 4 21 3.520256298260783 4 22 3.2158010677880853 4 23 2.9611388216363537 4 24 3.2291469665100863 4 25 3.291897132345909 4 26 2.399433072680843 4 27 2.1589737992736957 4 28 2.156141081870352 4 29 2.940480402752236 4 30 2.6534503873846162 4 31 1.1040385879402308 4 32 1.9874086143245933 4 33 1.4322145789757244 4 34 1.1726888910617106 4 35 1.6154757628545866 4 36 2.1271131937800334 4 37 2.545420555493642 4 38 2.2653146383400156 4 39 2.9856490378321965 4 40 6.521065767901359 4 41 8.730298944311205 4 42 5.039198333681107 4 43 6.375948522236431 4 44 4.951998683123314 4 45 4.171018205390272 4 46 4.670817204756698 4 47 3.811872442653552 4 48 4.783120767730463 4 49 6.478702241061814 4 50 4.462807613406778 4 51 3.4961267882102014 4 52 3.8213077869788776 4 53 3.9956323190084184 4 54 4.54216447329032 4 55 3.600656942079379 4 56 3.7969082627111383 5 1 1.0569738246334168 5 2 2.6692503881471055 5 3 1.7405881282874276 5 4 1.4556111683952224 5 5 1.5534957012585338 5 6 1.6856642219203906 5 7 1.3986641315164334 5 8 1.2491580561384836 5 9 1.2923727766839619 5 10 1.55504444401123 5 11 3.0607988521118514 5 12 4.1937882423014585 5 13 4.176723772305991 5 14 4.534997824857129 5 15 4.728667572987526 5 16 4.922302864693009 5 17 5.483913458690523 5 18 5.4434104434141375 5 19 4.430344124664437 5 20 4.505227831401996 5 21 3.5698835116811054 5 22 3.8041514871729873 5 23 3.165612260592553 5 24 3.621691234916419 5 25 3.299865445486631 5 26 2.5813995218886703 5 27 2.7789644796092006 5 28 2.7908102337808813 5 29 3.138336879919059 5 30 3.4130962401339175 5 31 2.3574402654798376 5 32 2.915600599413132 5 33 2.5404624820512183 5 34 2.390629245208963 5 35 2.756528884942192 5 36 3.546311702633716 5 37 3.871422419426537 5 38 4.061773165833024 5 39 4.853729186946829 5 40 8.095856075450289 5 41 9.807465766305615 5 42 6.620372611407106 5 43 7.589508634072406 5 44 7.557124498593957 5 45 6.530104878270048 5 46 6.4183474936663 5 47 6.280744763250013 5 48 5.870306229053856 5 49 8.124461793717916 5 50 5.803581145559615 5 51 5.170031727632377 5 52 4.400198746904319 5 53 4.988083390769834 5 54 5.513832693234399 5 55 4.2744686848566396 5 56 4.165873779952516 6 1 1.067318652219619 6 2 2.978856299477151 6 3 1.566696502671661 6 4 1.8272860452425093 6 5 1.7709833859875914 6 6 1.6612799854671891 6 7 1.3811764467873715 6 8 1.3806851497345463 6 9 1.5300575057480046 6 10 1.8740099612526109 6 11 3.1376461427522457 6 12 4.985906468902442 6 13 4.553078452108949 6 14 5.16849536180294 6 15 4.54837200648506 6 16 4.9587560666634545 6 17 5.122829714848159 6 18 5.464100098586556 6 19 4.813102745354094 6 20 4.450794333865048 6 21 3.7287505067549915 6 22 4.174102226089239 6 23 3.3377797482772777 6 24 4.063316850679968 6 25 3.4281905686393443 6 26 2.735340408588179 6 27 2.6213290116289087 6 28 2.4090368347661055 6 29 3.1536078158796528 6 30 3.2872341711684 6 31 2.1158146497162917 6 32 2.834319811235794 6 33 2.054994501755651 6 34 2.4603336787064976 6 35 2.3621939095727384 6 36 3.4896614563282924 6 37 3.6100923701654537 6 38 3.767438190463565 6 39 4.173679925863084 6 40 7.734772331607925 6 41 3.279140643152907 6 42 4.9538701483529195 6 43 6.270543116831025 6 44 5.263774744899379 6 45 4.510400444772518 6 46 4.530662764602258 6 47 5.149710280491391 6 48 4.971537756147452 6 49 6.864609576969148 6 50 4.644467845067009 6 51 4.862149954233367 6 52 4.770149485820578 6 53 5.224782898158995 6 54 4.873438604564452 6 55 4.547375088797526 6 56 5.061193976996854 7 1 1.0636149485159194 7 2 2.6669515375723876 7 3 1.2614054973806574 7 4 1.278344246300712 7 5 1.4638405288447345 7 6 1.138793212980417 7 7 0.9623933780043004 7 8 1.0923253613747548 7 9 1.0816448073353016 7 10 1.3277136649563133 7 11 2.635000640106739 7 12 4.28114456414054 7 13 4.164718663749156 7 14 4.522992716300294 7 15 4.606573064686117 7 16 5.109020616928007 7 17 5.453517545535988 7 18 5.730766765253222 7 19 4.50569533794669 7 20 4.133069466140178 7 21 3.0993854273899117 7 22 3.430715982702999 7 23 2.781959642457174 7 24 3.3820999194630375 7 25 3.0252805157292926 7 26 2.6043880276357996 7 27 2.22238721268711 7 28 2.4730579987872687 7 29 2.747787710059548 7 30 2.5372341711684 7 31 1.693327877229521 7 32 1.8729441498601318 7 33 2.0150474118085597 7 34 1.3277939961668181 7 35 2.0275378249166494 7 36 1.9917778584446992 7 37 2.36644157651466 7 38 2.162411735437111 7 39 2.583997386180542 7 40 5.428423125258721 7 41 0.3931618071740708 7 42 3.541700836183608 7 43 4.290913487201397 7 44 4.074621305745936 7 45 4.029977164349237 7 46 2.7846310185705114 7 47 3.0978584286395403 7 48 2.887675322285016 7 49 4.978101640461212 7 50 3.3402350408342016 7 51 3.3018324939159065 7 52 3.745281760952853 7 53 3.2271638505399487 7 54 4.244867175993022 7 55 3.6923486337710685 7 56 2.919130484933362 8 1 0.9212869061878735 8 2 2.333618204239059 8 3 0.8428869788621398 8 4 0.9439527119091764 8 5 0.6876500526542628 8 6 1.0462006203878218 8 7 1.0200653356762572 8 8 0.8600502290996204 8 9 0.9705336962241944 8 10 1.0017877390303909 8 11 2.5820905871966886 8 12 4.422414405410379 8 13 4.103872102902599 8 14 4.533574726882307 8 15 4.610276768389824 8 16 4.8174862253936155 8 17 5.076797968816415 8 18 5.070449304935764 8 19 4.38929322154457 8 20 3.632540365611078 8 21 3.25652828453277 8 22 3.762991114978128 8 23 3.0227003831979147 8 24 3.2799835173466363 8 25 2.851206441655215 8 26 2.7154991387469067 8 27 2.3118052021050985 8 28 2.2979257236549913 8 29 2.480591942863782 8 30 2.841996075930304 8 31 1.9912114751131185 8 32 2.5057483826643647 8 33 1.9303913271524777 8 34 1.9246193929922129 8 35 1.9407653381441676 8 36 2.0563281229949624 8 37 1.6458066558797384 8 38 1.7618826349080123 8 39 2.790346592529751 8 40 4.564931061766654 8 41 -0.5787958647836007 8 42 3.996727291210064 8 43 5.1649875612754705 8 44 4.934409665534297 8 45 3.5056385400106116 8 46 4.087805621745115 8 47 4.125900756681865 8 48 4.212013946623639 8 49 6.69556195792153 8 50 4.587324987924152 8 51 5.082784874868288 8 52 4.087609803280895 8 53 4.630338453714554 8 54 4.975025906151753 8 55 5.1918195332419685 8 56 5.1064320722349485 9 1 1.069574291317366 9 2 2.41381870549219 9 3 1.3328619162054949 9 4 1.2622484512575483 9 5 1.2202315062883429 9 6 1.091731113286734 9 7 0.7439584016745897 9 8 0.919782894095448 9 9 1.0808093854472531 9 10 0.43453627704208486 9 11 2.107570954782318 9 12 4.076132032812218 9 13 3.9756348430863895 9 14 4.318871301652564 9 15 3.9874697508459604 9 16 4.685071856137135 9 17 4.852904902818082 9 18 4.871618895579033 9 19 3.6649824446022166 9 20 4.194779296271065 9 21 3.189276822544471 9 22 4.001921774961417 9 23 3.3163511768487055 9 24 3.4892567003040256 9 25 3.0195439520979903 9 26 2.9928592055806575 9 27 2.6514041995988293 9 28 2.7586608949164813 9 29 3.1393221015939403 9 30 2.674076276431556 9 31 2.086867281295241 9 32 2.0320641721380497 9 33 2.0222877348383577 9 34 1.9020630020147706 9 35 1.9031713531817616 9 36 1.9310148397869398 9 37 2.4277615430977875 9 38 2.3587915739222183 9 39 3.093604737893159 9 40 4.564095639878598 9 41 3.278764703303281 9 42 4.2072536069995365 9 43 5.141595748409973 9 44 4.4836995569294515 9 45 3.6322049560507104 9 46 4.091565020241358 9 47 3.8339208068071784 9 48 3.536575350132413 9 49 5.83340656945035 9 50 4.425670852585807 9 51 3.2223003301732156 9 52 2.961878809128848 9 53 3.4732791387604998 9 54 4.500506273737383 9 55 4.078202156466698 9 56 3.3570586386509866 10 1 0.739837736189088 10 2 2.1071550999133564 10 3 1.1240574623226962 10 4 1.1464245403352038 10 5 1.0836878570890107 10 6 1.1272014685642446 10 7 0.908166632174499 10 8 1.039681878196923 10 9 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29 1.4846637165007692 116 30 1.285060258124922 116 31 0.7751314199026282 116 32 1.7132017988134294 116 33 1.0813920172835978 116 34 0.7519485855387984 116 35 0.9022560213739794 116 36 1.8281707730984778 116 37 2.03214205960645 116 38 1.633587258786548 116 39 2.460325888596003 116 40 6.006200903036497 116 41 3.817960518929304 116 42 -1.0132105969886958 116 43 -8.99250036142984 116 44 -7.316038919696897 116 45 0.42965510315760724 116 46 -2.7612627012362516 116 47 -2.0676810238564336 116 48 -3.077220007827705 116 49 -5.573589180794826 116 50 -2.167333397169017 116 51 -1.0614525302386824 116 52 -1.210595855794331 116 53 -2.8916767291701966 116 54 -6.824698041398282 116 55 -1.706041060270799 116 56 -1.6161352155497326 117 1 -0.8088718239708541 117 2 1.3693324899533437 117 3 0.4143155502907092 117 4 0.2772860452425121 117 5 0.00907862408283222 117 6 0.11366093784813813 117 7 -0.0950140294031101 117 8 -0.15979104074164496 117 9 -0.13660916091866682 117 10 0.557343294585948 117 11 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119 14 -12.248294034367898 119 15 -9.42924213195382 119 16 -13.131376481201052 119 17 -15.296463363207806 119 18 -16.362704025124785 119 19 -5.5611240587696145 119 20 -7.084036299419193 119 21 0.05975197950904487 119 22 1.68264419957967 119 23 1.332551471399519 119 24 0.4829781172484502 119 25 1.0923290075200498 119 26 2.116415519044729 119 27 2.347396758315213 119 28 2.5629396329987983 119 29 2.669513559620448 119 30 2.5661001505498433 119 31 1.3270812181993534 119 32 -4.507506403786298 119 33 1.7294422189868754 119 34 1.659523663978959 119 35 0.4989391231220708 119 36 1.2850959187730666 119 37 1.4381483348193598 119 38 1.5052143318479594 119 39 1.7775827240957796 119 40 5.478365851490107 119 41 3.15520838983921 119 42 -0.7794140931787439 119 43 -9.568116676983404 119 44 -4.474443222700035 119 45 -4.085181293077266 119 46 -12.23988215439627 119 47 -7.654413430848816 119 48 -10.083764158432814 119 49 -7.987305003295948 119 50 -7.062895925183362 119 51 -6.007216761525104 119 52 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34 -1.7529867846139666 120 35 -1.648037750658922 120 36 -1.562075995409156 120 37 -0.9951199850468981 120 38 -1.0995502265248476 120 39 -1.0886752864921263 120 40 2.0007954976310884 120 41 0.14921786323012753 120 42 -4.271612477129704 120 43 -12.198375802087892 120 44 -7.99394726282263 120 45 -8.964116929744861 120 46 -12.058912524973032 120 47 -12.134073503292392 120 48 -10.507419772810078 120 49 -17.534811272451698 120 50 -10.35475995416079 120 51 -8.679548887465476 120 52 -8.99625978058869 120 53 -10.666529843153747 120 54 -11.119997688871843 120 55 -9.23718089575846 120 56 -5.95984849404562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 280.0 5 44.0 6 74.0 7 135.0 8 246.0 9 242.0 10 173.0 11 170.0 12 157.0 13 133.0 14 180.0 15 231.0 16 319.0 17 425.0 18 570.0 19 658.0 20 726.0 21 1032.0 22 1278.0 23 1585.0 24 1849.0 25 2119.0 26 2279.0 27 2510.0 28 2891.0 29 3367.0 30 3977.0 31 4782.0 32 5476.0 33 6175.0 34 7383.0 35 8815.0 36 10906.0 37 13720.0 38 13276.0 39 8311.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.16999201915402 0.6084221398056429 0.1502276888408995 0.07135815219942726 8 97.85549974179617 1.6571991925261726 0.21970799492981552 0.2675930707478522 9 91.05112436035867 5.641988639031031 2.0271348762968873 1.2797521243134125 10 34.51575043425191 39.41692878268626 18.33810619219755 7.729214590864279 11 26.223182010234265 41.337026430684006 15.779540866625979 16.66025069245575 12 30.479320219707994 20.978357823576356 17.165391296183277 31.37693066053237 13 19.717714672075726 18.8265344451957 21.57144466982195 39.884306212906615 14 24.368327652742174 22.973061791686618 30.5024271616762 22.156183393895006 15 32.895467334130025 20.523557253488654 20.218682073527614 26.36229333885371 16 33.55380597155151 26.685018508427444 20.767958811702588 18.993216708318457 17 28.405239190648324 22.986714238768133 28.519787803389512 20.088258767194027 18 24.50236610831518 24.37936603320063 29.935964846390746 21.182303012093442 19 35.335024903674466 27.30758387369608 18.003007236162013 19.35438398646744 20 20.58566474640303 39.19029405407438 19.785920363878994 20.438120835643602 21 21.029998591615417 26.026947091685837 33.43035538237641 19.51269893432233 22 24.949063424252383 25.686118022628047 20.34552368433407 29.019294868785504 23 22.464673020046007 29.944134078212294 19.808459696727855 27.782733205013848 24 26.065442936951317 24.69837096849913 29.94788977043331 19.28829632411624 25 31.91395466751798 26.853955794257384 21.604289121331057 19.627800416893578 26 21.681361916298112 23.34525855188409 24.923706771082756 30.049672760735046 27 25.409011887421883 22.539115726166422 22.67067612648593 29.381196259925762 28 24.696847210994342 34.08847361395725 23.596097084093174 17.618582090955236 29 33.039328956143564 25.48726763791435 24.879533350240933 16.59387005570115 30 20.63894523326572 26.034858387799563 36.623844940274964 16.702351438659754 31 19.084693551719603 38.16187381430906 27.926895696763648 14.826536937207685 32 28.285856601787547 27.551854740937753 28.496376981852016 15.665911675422686 33 18.01094478712201 28.05588987513164 30.55325710846999 23.379908229276367 34 15.582317245007232 37.6153080203656 33.411614406222405 13.390760328404758 35 15.620890475295887 29.64963366522638 43.082848018034944 11.646627841442795 36 15.729366242157857 38.81062399038281 31.88230211503062 13.577707652428717 37 16.73940442822476 28.68470061513573 41.710716905886116 12.8651780507534 38 26.354093113025467 29.132420091324203 31.279009556089065 13.234477239561269 39 17.30112594181223 28.014974932039017 31.79915530848172 22.884743817667033 40 12.681967709002912 20.75377169433206 36.82553786818921 29.73872272847582 41 12.21112426035503 19.39408284023669 34.22485207100592 34.16994082840237 42 23.331860362350863 27.558973683963107 33.322364400485135 15.786801553200892 43 13.03865011622326 23.454512944542213 40.825890966582286 22.680945972652246 44 13.357172510095483 24.108389629014606 32.03664565799902 30.497792202890896 45 16.070454075007998 27.388457123501624 42.04568976872848 14.495399032761897 46 14.593857297398994 28.206575419782702 33.19034852546917 24.00921875734914 47 14.207881264384012 35.50796111032831 33.778591893288244 16.505565731999436 48 16.218809078168942 36.88829111840558 33.24742995269044 13.645469850735036 49 13.11501718083299 29.05637578824151 29.52460068723332 28.30400634369218 50 23.456743696293927 28.61440685933475 33.401650903484196 14.527198540887126 51 14.800934121812498 37.34603939884742 33.49184526724171 14.361181212098383 52 14.228227042375352 28.816555251553698 41.92310946887429 15.032108237196665 53 14.888134445562526 28.864980767964788 41.64088288678021 14.606001899692474 54 13.254588235294118 34.98729411764706 31.997176470588233 19.76094117647059 55 14.667883658125772 37.94284531348508 32.82207145747273 14.567199570916415 56 24.045607631375944 29.023123671188543 31.822800052682084 15.108468644753428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 5.0 5 10.0 6 24.0 7 38.0 8 212.0 9 386.0 10 771.5 11 1157.0 12 1319.0 13 1294.5 14 1108.0 15 933.5 16 759.0 17 809.0 18 859.0 19 1117.5 20 1376.0 21 1479.0 22 1710.0 23 1838.0 24 2149.5 25 2461.0 26 2892.5 27 3324.0 28 3593.5 29 4121.5 30 4380.0 31 4583.5 32 4787.0 33 4572.0 34 4357.0 35 3924.0 36 3491.0 37 2909.5 38 2015.5 39 1703.0 40 1631.0 41 1559.0 42 1573.5 43 1588.0 44 1876.5 45 2165.0 46 4409.5 47 6294.0 48 5934.0 49 4749.0 50 3564.0 51 3395.0 52 3226.0 53 3517.5 54 3858.5 55 3908.0 56 3994.5 57 4081.0 58 4372.5 59 4664.0 60 4790.0 61 4916.0 62 4911.5 63 4445.5 64 3984.0 65 3506.0 66 3028.0 67 2587.0 68 2146.0 69 1889.5 70 1633.0 71 1414.5 72 1067.5 73 939.0 74 756.5 75 574.0 76 453.5 77 333.0 78 278.0 79 170.0 80 117.0 81 81.0 82 45.0 83 28.5 84 12.0 85 9.5 86 7.0 87 3.5 88 0.5 89 1.0 90 1.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.01596169193934557 14 0.0018778461105112435 15 0.21783014881930426 16 0.06290784470212667 17 0.0 18 9.389230552556217E-4 19 0.08919769024928408 20 0.0901366133045397 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0028167691657668657 26 0.007511384442044974 27 0.08450307497300596 28 0.1154875357964415 29 0.041312614431247355 30 0.01596169193934557 31 0.025350922491901788 32 0.09577015163607343 33 0.14365522745411013 34 0.047885075818036714 35 0.04225153748650298 36 0.027228768602413033 37 0.17557861133280128 38 0.2722876860241303 39 0.18215107271959063 40 0.6732078306182808 41 0.8262522886249473 42 0.13520491995680955 43 0.22909722548237174 44 0.4854232195671565 45 0.20937984132200368 46 0.18778461105112437 47 0.04694615276278109 48 0.1727618421670344 49 0.5380029106614713 50 0.13051030468053143 51 0.29012722407398717 52 0.13708276606732078 53 0.16149476550396694 54 0.23942537909018355 55 0.21783014881930426 56 0.19435707243791372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 106505.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.75930707478523 #Duplication Level Percentage of deduplicated Percentage of total 1 86.93004416190824 58.03389512229473 2 7.389384264858935 9.866203464626073 3 2.388118477679953 4.7828740434721375 4 1.1631177744648533 3.105957466785597 5 0.5794492419341228 1.9341814938265807 6 0.3333239571320075 1.3351485845734943 7 0.26018958679080756 1.2159053565560303 8 0.16736519366543837 0.8938547486033519 9 0.10407583471632303 0.6253227548002441 >10 0.6146099969058536 7.268203370733768 >50 0.035160754971730754 1.511666118961551 >100 0.029535034176253836 3.642082531336557 >500 0.00421929059660769 2.497535326979954 >1k 0.00140643019886923 3.2871696164499324 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 3501 3.2871696164499324 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 972 0.9126332097084643 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 924 0.8675649030561946 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 764 0.7173372142152951 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 428 0.4018590676494061 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 418 0.3924698370968499 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 276 0.25914276325055163 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 268 0.25163137880850667 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 268 0.25163137880850667 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 247 0.23191399464813858 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 179 0.16806722689075632 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 178 0.16712830383550067 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 177 0.16618938078024506 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 168 0.15773907328294445 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 145 0.13614384301206514 No Hit TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 139 0.13051030468053143 No Hit TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 137 0.12863245857002018 No Hit TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.10891507440965213 No Hit TAGTCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 114 0.10703722829914089 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA 108 0.10140368996760715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.389230552556218E-4 0.0 0.0 0.0 0.0 8 9.389230552556218E-4 0.0 0.0 0.0 0.0 9 9.389230552556218E-4 0.0 0.0 0.0 0.0 10 0.006572461386789352 0.0 0.0 0.0 0.0 11 0.013144922773578704 0.0 0.0 0.0 0.0 12 0.018778461105112436 0.0 0.0 0.0 0.0 13 0.02065630721562368 0.0 0.0 0.0 0.0 14 0.03098446082343552 0.0 0.0 0.0 0.0 15 0.03661799915496925 0.0 0.0 0.0 0.0 16 0.04225153748650298 0.0 0.0 0.0 0.0 17 0.049762921928547955 0.0 0.0 0.0 0.0 18 0.05727430637059293 0.0 0.0 0.0 0.0 19 0.06384676775738228 0.0 0.0 0.0 0.0 20 0.07793061358621661 0.0 0.0 0.0 0.0 21 0.08450307497300596 0.0 0.0 0.0 0.0 22 0.0948312285808178 0.0 0.0 0.0 0.0 23 0.11360968968593024 0.0 0.0 0.0 0.0 24 0.12205999718323084 0.0 0.0 0.0 0.0 25 0.12957138162527582 0.0 0.0 0.0 0.0 26 0.13614384301206517 0.0 0.0 0.0 0.0 27 0.14177738134359888 0.0 0.0 0.0 0.0 28 0.15304445800666636 0.0 0.0 0.0 0.0 29 0.15680015022768884 0.0 0.0 0.0 0.0 30 0.16243368855922258 0.0 0.0 0.0 0.0 31 0.16712830383550067 0.0 0.0 0.0 0.0 32 0.17088399605652316 0.0 0.0 0.0 0.0 33 0.17463968827754567 0.0 0.0 0.0 0.0 34 0.1765175343880569 0.0 0.0 0.0 0.0 35 0.1774564574433125 0.0 0.0 0.0 9.389230552556218E-4 36 0.18215107271959063 0.0 0.0 0.0 9.389230552556218E-4 37 0.18590676494061312 0.0 0.0 0.0 9.389230552556218E-4 38 0.18778461105112437 0.0 0.0 0.0 9.389230552556218E-4 39 0.1896624571616356 0.0 0.0 0.0 9.389230552556218E-4 40 0.19435707243791372 0.0 0.0 0.0 9.389230552556218E-4 41 0.1981127646589362 0.0 0.0 0.0 9.389230552556218E-4 42 0.20562414910098117 0.0 0.0 0.0 9.389230552556218E-4 43 0.21031876437725927 0.0 0.0 0.0 9.389230552556218E-4 44 0.21876907187455988 0.0 0.0 0.0 9.389230552556218E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTGAC 15 0.00802276 50.59952 38 ACACCCG 15 0.008037918 50.575382 40 CCACTGA 15 0.008037918 50.575382 40 TTCCACC 15 0.008037918 50.575382 39 TCGTCTC 95 0.0 50.575382 40 CTTGACT 25 1.9386393E-5 50.575382 39 TAGTCTC 20 3.9376804E-4 50.57538 40 CACCGAA 15 0.008053098 50.551266 41 TATGCCT 100 0.0 50.383083 35 TATGCCC 20 4.0124683E-4 50.38308 35 ATGCCTG 90 0.0 50.383076 36 ATATGCA 15 0.008236921 50.263634 34 TGTATAT 15 0.008314428 50.14475 48 TCCACGA 35 5.129732E-8 50.12104 45 CAAGGAG 15 0.008423848 49.979256 33 AAGGTCC 15 0.008455306 49.93217 27 TGTTCAC 15 0.008455306 49.93217 14 ATGGTTG 15 0.008486852 49.885174 13 AGTATGG 15 0.0085026575 49.861713 19 ATCAAAG 15 0.0085026575 49.861713 17 >>END_MODULE