FastQCFastQC Report
Fri 17 Jun 2016
SRR1526193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16820
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGTCTCTGATGTTGGGGTTAGTGCCTTAGAGGGTACTGGGAGTCCAGC240.1426872770511296No Hit
TCGTAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT220.13079667063020214No Hit
TCGTAAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1248513674197384No Hit
TCGTAAGGGAGTCTCTTGTCTCAGGCTCTCTGCCTTGTTATCAGTCTTAAGCATAT210.1248513674197384No Hit
TCGTAAGGGCACTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11890606420927466No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.11890606420927466No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC190.11296076099881094No Hit
TCGTAAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1070154577883472No Hit
TCGTAAGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1070154577883472No Hit
TCGTAAGGGGTTTCCTATTGGGCAAAGTTAGTGTAAAAGTGACTTTCCTTTTTTTT180.1070154577883472No Hit
TCGTAAGGGTGGCACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10107015457788349No Hit
TCGTAAGGGGTTGTTGTTGTTTTGGTTTGGTTTGTTCTGTTCTGTTTTTTGAGATA170.10107015457788349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAA150.00800817250.43373537
AACCAAA150.00800817250.43373538
CCCCCGA150.00800817250.43373540
GCCCCCG150.00800817250.43373539
AGAGCCC150.008104376550.28228436
AAAACCA150.008104376550.28228436
AAAAACC150.008104376550.28228435
AATTCGT150.00829936249.98209413
CCACGCA150.00829936249.98209414
ATGGTAT150.00829936249.98209414
GAATGTA150.00829936249.9820949
GAGCATG150.00829936249.9820949
ATTCGTG150.00829936249.98209414
ACCCCCC150.00829936249.98209411
GAATTCG150.00829936249.98209412
TATCACG150.00829936249.98209414
GATCACG150.00829936249.98209411
CATGGTA150.00829936249.98209413
AATGTAT150.00829936249.98209410
AGATCAC150.00829936249.98209410
Produced by FastQC (version 0.11.5)