##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526193_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16820 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.19256837098692 38.0 37.0 39.0 33.0 40.0 2 36.699881093935794 38.0 36.0 39.0 31.0 40.0 3 36.43644470868014 38.0 35.0 39.0 30.0 40.0 4 36.58692033293698 38.0 36.0 39.0 30.0 40.0 5 34.52092746730083 38.0 35.0 39.0 17.0 40.0 6 33.747146254458976 38.0 33.0 39.0 17.0 40.0 7 36.52461355529132 38.0 36.0 39.0 30.0 40.0 8 36.56771700356718 38.0 36.0 39.0 30.0 40.0 9 36.524791914387635 38.0 36.0 39.0 31.0 40.0 10 35.934661117717006 38.0 35.0 39.0 29.0 40.0 11 35.45713436385255 38.0 34.0 39.0 28.0 40.0 12 33.54030915576694 38.0 31.0 39.0 20.0 40.0 13 33.13757431629013 37.0 31.0 39.0 19.0 40.0 14 33.45529131985731 38.0 31.0 39.0 21.0 40.0 15 33.42473246135553 38.0 31.0 39.0 20.0 40.0 16 33.6845422116528 38.0 32.0 39.0 22.0 40.0 17 33.52128418549346 37.0 31.0 39.0 22.0 40.0 18 32.979964328180735 36.0 30.0 39.0 19.0 40.0 19 33.94310344827586 37.0 32.0 39.0 24.0 40.0 20 33.98133174791914 37.0 32.0 38.0 24.0 40.0 21 34.75790725326992 38.0 33.0 39.0 26.0 40.0 22 34.36831153388823 38.0 33.0 39.0 25.0 40.0 23 35.14369797859691 38.0 34.0 39.0 27.0 40.0 24 34.759750297265164 38.0 33.0 39.0 26.0 40.0 25 35.03281807372176 38.0 34.0 39.0 27.0 40.0 26 35.62806183115339 38.0 35.0 39.0 29.0 40.0 27 35.62907253269917 38.0 35.0 39.0 29.0 40.0 28 35.5987514863258 38.0 35.0 39.0 29.0 40.0 29 35.41985731272295 38.0 35.0 39.0 28.0 40.0 30 35.33115338882283 38.0 35.0 39.0 28.0 40.0 31 35.6910820451843 38.0 35.0 39.0 29.0 40.0 32 35.32366230677765 38.0 35.0 39.0 28.0 40.0 33 35.536266349583826 38.0 35.0 39.0 29.0 40.0 34 35.49221165279429 38.0 35.0 39.0 29.0 40.0 35 35.317241379310346 38.0 35.0 39.0 29.0 40.0 36 34.775089179548154 38.0 34.0 39.0 29.0 40.0 37 34.47972651605232 38.0 33.0 39.0 28.0 39.0 38 34.13626634958383 37.0 33.0 39.0 27.0 39.0 39 33.63501783590963 37.0 33.0 39.0 24.0 39.0 40 30.196135552913198 35.0 26.0 38.0 10.0 39.0 41 26.948275862068964 32.0 16.0 38.0 6.0 39.0 42 31.16652794292509 35.0 29.0 38.0 14.0 39.0 43 29.77728894173603 34.0 27.0 38.0 7.0 39.0 44 29.655231866825208 35.0 27.0 38.0 5.0 39.0 45 30.654518430439953 35.0 29.0 38.0 4.0 39.0 46 30.196254458977407 35.0 28.0 38.0 4.0 39.0 47 29.663139120095124 35.0 27.0 38.0 4.0 39.0 48 29.757847800237812 35.0 27.0 38.0 4.0 39.0 49 27.09024970273484 33.0 16.0 38.0 4.0 39.0 50 29.330321046373363 35.0 26.0 38.0 4.0 39.0 51 29.494470868014268 35.0 27.0 39.0 4.0 40.0 52 29.256242568370986 35.0 26.0 39.0 4.0 40.0 53 28.93834720570749 35.0 26.0 39.0 4.0 40.0 54 27.844768133174792 35.0 18.0 39.0 4.0 40.0 55 28.10065398335315 35.0 23.0 38.0 4.0 39.0 56 26.332639714625447 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3142563266913 1 2 1.8653776784146459 1 3 2.0445135612295076 1 4 2.110634021350741 1 5 4.797251388825892 1 6 5.896848086561139 1 7 2.1882748059545563 1 8 1.9857704760266728 1 9 1.8822326144930628 1 10 2.6591740352512616 1 11 -1.135181893699702 1 12 -11.597264832568829 1 13 -9.685776193226864 1 14 -2.7057761468407797 1 15 -4.842686562942568 1 16 -21.65247522859694 1 17 -3.683002966804459 1 18 -13.408545356110182 1 19 -5.182230372253592 1 20 -5.194673008421454 1 21 -7.65722428936304 1 22 -3.5710211346029794 1 23 -4.387525598183494 1 24 -4.557330518115947 1 25 -4.819269764055164 1 26 1.2957330272067153 1 27 1.338508503085329 1 28 0.8484652623778004 1 29 2.1428638247144534 1 30 1.6483917346126944 1 31 1.7160685066925438 1 32 2.2392655818840055 1 33 0.008933710424415153 1 34 0.5714767375011149 1 35 1.5304990853588123 1 36 0.8372060261433205 1 37 2.036485950961385 1 38 1.4730389598146942 1 39 1.305475225031536 1 40 3.8525949154904424 1 41 -7.15697022802906 1 42 -5.625679305336618 1 43 -17.27722482222658 1 44 -20.72577110042376 1 45 -8.698362195173523 1 46 -13.877543059279905 1 47 -22.42029777651256 1 48 -12.253492656122685 1 49 -16.91663976651573 1 50 -9.696539673440036 1 51 -5.0144060817779845 1 52 -3.91896911599466 1 53 -4.815858255521565 1 54 -3.9007457936943304 1 55 -4.034857171681079 1 56 1.647283363516138 2 1 1.9809229933579644 2 2 2.5002983133352856 2 3 2.6318151485310963 2 4 2.6344435451602664 2 5 5.09090218247669 2 6 6.388911578624633 2 7 2.767639885319639 2 8 2.239738729994933 2 9 2.596518328778778 2 10 2.976634352711578 2 11 1.1822784237606143 2 12 -2.1052013405053387 2 13 0.584065076614408 2 14 3.198985757921122 2 15 2.2604880402320333 2 16 2.371334295212584 2 17 2.8884256046241106 2 18 -2.3291802767451024 2 19 1.722531532508313 2 20 2.3688190550706096 2 21 0.43007729793854566 2 22 2.1829471193652736 2 23 -0.33197004262793683 2 24 0.6648917041062745 2 25 0.2680318232464245 2 26 1.930653662127348 2 27 0.2512069157837402 2 28 1.7452906592031923 2 29 1.476197158047789 2 30 0.680137766358726 2 31 2.271624062248094 2 32 2.52497986759829 2 33 2.262901964392668 2 34 2.5079846740090517 2 35 2.26065781551754 2 36 2.567364756302048 2 37 3.3063272208026504 2 38 3.8460548328305677 2 39 3.099126018682327 2 40 5.043071105966632 2 41 -0.029986101044933378 2 42 -2.062187241844555 2 43 -7.88039942540118 2 44 -11.265453640106301 2 45 -2.3967748935862225 2 46 -9.012463694200541 2 47 -19.10283745905224 2 48 -6.705873608503637 2 49 -11.60711595699192 2 50 -4.704476181376542 2 51 -0.6175806849525891 2 52 0.03341183638629275 2 53 -0.5380804777437866 2 54 0.7738573809088471 2 55 0.5762539394300319 2 56 5.242521458754233 3 1 0.99607450850948 3 2 1.840125153162127 3 3 1.5647155814315283 3 4 1.8379067486234675 3 5 4.852806944381449 3 6 5.826141015854073 3 7 2.0620121796919335 3 8 1.960517950774154 3 9 2.0792023114627654 3 10 0.6086689847462097 3 11 2.0567372982194883 3 12 3.786573551269555 3 13 2.1273551199044505 3 14 2.9457390046743726 3 15 3.470444750188747 3 16 3.8626762865545743 3 17 3.862451578650088 3 18 3.13185868429386 3 19 1.9995878095645878 3 20 1.1588623451138957 3 21 1.0902504581117043 3 22 2.146150582568737 3 23 1.6528784422205476 3 24 0.8164068556214303 3 25 0.04941710463170779 3 26 3.0583592898329783 3 27 2.181942846519668 3 28 0.24745516136769652 3 29 -0.5389543571037265 3 30 0.8403109265318847 3 31 1.6504119410359763 3 32 1.860477703096123 3 33 2.5139842154749203 3 34 -0.34266467664029676 3 35 0.15676171162143504 3 36 1.7058928948301926 3 37 1.9708293853048175 3 38 0.008392495168230596 3 39 2.6943641139204217 3 40 6.367746430641958 3 41 2.459191388132556 3 42 1.3036136239563092 3 43 -6.544901589903347 3 44 -1.1399125145651752 3 45 -0.041796538607865585 3 46 -2.79168447342132 3 47 -6.304136160350943 3 48 -1.5716744743045012 3 49 -0.4014882513642135 3 50 -1.7369437138440738 3 51 0.7027656353937317 3 52 0.4295157324901915 3 53 0.47707103740772894 3 54 1.0639006709521368 3 55 -1.4540490908729993 3 56 -0.6860499698171942 4 1 2.074256326691298 4 2 2.3764887895257587 4 3 2.475624672340622 4 4 2.870634021350739 4 5 5.732806944381451 4 6 6.887959197672256 4 7 3.2038303615101142 4 8 3.056881587137788 4 9 3.095565947826401 4 10 3.161396257473484 4 11 2.8203736618558537 4 12 6.073846278542284 4 13 5.336446028995354 4 14 5.547557186492554 4 15 5.759535659279656 4 16 3.7008581047363975 4 17 3.6769970331955406 4 18 4.013676866112043 4 19 3.817769627746408 4 20 4.669771436022991 4 21 3.433886821748068 4 22 2.9334233098414657 4 23 2.3346966240387275 4 24 4.038225037439609 4 25 3.618508013722611 4 26 2.5801774716511616 4 27 0.6473973919742164 4 28 1.992909706822246 4 29 3.7028638247144556 4 30 1.4039472901682544 4 31 2.204957395581431 4 32 1.3859322485506667 4 33 2.346711488202196 4 34 1.1536989597233358 4 35 2.3749435298032537 4 36 1.9349838039210994 4 37 2.2853748398502702 4 38 2.2974834042591397 4 39 4.474364113920423 4 40 6.345928248823775 4 41 11.451918660859828 4 42 5.625431805774493 4 43 8.340552955551203 4 44 7.89645112179846 4 45 5.772748915937587 4 46 5.204679162942316 4 47 5.748591112376332 4 48 5.966507343877314 4 49 7.556693566817604 4 50 3.5412381043377437 4 51 4.7100383626664595 4 52 4.827697550672006 4 53 4.995252855589548 4 54 6.034809761861229 4 55 5.069587272763361 4 56 8.453950030182806 5 1 2.2142563266912987 5 2 2.2764887895257573 5 3 2.5056246723406232 5 4 2.360634021350741 5 5 5.302806944381452 5 6 6.707959197672256 5 7 2.893830361510112 5 8 2.396881587137784 5 9 2.8155659478264 5 10 3.18139625747348 5 11 2.7703736618558565 5 12 5.66384627854228 5 13 6.136446028995358 5 14 6.077557186492555 5 15 6.329535659279657 5 16 4.930858104736394 5 17 4.266997033195544 5 18 5.31367686611204 5 19 3.867769627746405 5 20 3.4997714360229892 5 21 3.8038868217480726 5 22 3.723423309841465 5 23 3.684696624038729 5 24 3.24822503743961 5 25 3.858508013722613 5 26 2.5901774716511596 5 27 2.7273973919742147 5 28 3.0429097068222433 5 29 3.4428638247144505 5 30 2.60394729016825 5 31 1.6049573955814296 5 32 2.40593224855067 5 33 2.4367114882021923 5 34 1.5936989597233335 5 35 1.674943529803258 5 36 2.814983803921102 5 37 2.5753748398502694 5 38 3.4674834042591343 5 39 4.09436411392042 5 40 7.985928248823775 5 41 10.58191866085983 5 42 4.53543180577449 5 43 7.700552955551203 5 44 7.846451121798463 5 45 6.8627489159375905 5 46 7.7946791629423196 5 47 6.618591112376329 5 48 6.646507343877314 5 49 11.016693566817604 5 50 7.88123810433774 5 51 7.880038362666461 5 52 7.247697550672008 5 53 7.845252855589543 5 54 5.704809761861224 5 55 5.5095872727633655 5 56 4.013950030182805 6 1 2.1237801362151103 6 2 2.3574411704781397 6 3 1.6794341961501473 6 4 3.015395926112646 6 5 5.567092658667164 6 6 6.341292531005589 6 7 2.0533541710339236 6 8 2.049262539518736 6 9 2.0727088049692526 6 10 3.1671105431877677 6 11 3.0870403285225194 6 12 4.132893897589902 6 13 3.726922219471547 6 14 6.198985757921122 6 15 6.260488040232033 6 16 4.0380009618792485 6 17 5.316997033195541 6 18 5.575581628016799 6 19 4.293960103936882 6 20 4.36881905507061 6 21 1.049124916986166 6 22 4.563899500317653 6 23 2.763268052610158 6 24 2.283939323153895 6 25 3.4108889661035633 6 26 1.073510804984494 6 27 1.7273973919742147 6 28 1.031004944917484 6 29 2.1428638247144534 6 30 1.0134710996920617 6 31 0.5573383479623786 6 32 0.5249798675982902 6 33 1.6914733929640988 6 34 1.5556037216280956 6 35 2.117800672660401 6 36 3.1387933277306246 6 37 2.2110891255645555 6 38 1.2746262614019912 6 39 2.5753164948728013 6 40 4.947833010728537 6 41 0.20810913705030387 6 42 2.9854318057744926 6 43 1.31007676507501 6 44 2.782165407512746 6 45 2.984177487366157 6 46 4.178012496275652 6 47 4.087638731423951 6 48 2.8655549629249357 6 49 6.345264995389034 6 50 4.533619056718692 6 51 4.001466934095028 6 52 4.176268979243439 6 53 1.27144333178002 6 54 2.6786192856707522 6 55 1.8143491775252691 6 56 0.9568071730399481 7 1 1.9984668530070877 7 2 2.502804578999445 7 3 2.524045724972204 7 4 2.479055073982323 7 5 5.273859575960394 7 6 6.534274987145938 7 7 2.6648829930890585 7 8 2.2547763239798897 7 9 2.531355421510611 7 10 2.6708699416840105 7 11 4.104584188171643 7 12 6.250688383805439 7 13 5.7469723447848295 7 14 5.732820344387292 7 15 6.142693554016496 7 16 5.338752841578497 7 17 5.264365454248171 7 18 4.81367686611204 7 19 4.765138048799038 7 20 3.4866135412861468 7 21 2.006518400695441 7 22 2.162896994051991 7 23 3.124170308249255 7 24 3.7876987216501377 7 25 2.563771171617354 7 26 2.9507037874406308 7 27 3.569502655132112 7 28 2.7929097068222433 7 29 3.406021719451296 7 30 2.888157816484039 7 31 1.1839047640024845 7 32 3.0638269853927724 7 33 0.8393430671495636 7 34 2.530541064986494 7 35 2.55389089822431 7 36 1.4044574881316265 7 37 3.0832695766923734 7 38 3.522746562153877 7 39 4.404890429709894 7 40 7.606980880402723 7 41 2.468760766122987 7 42 4.511747595248178 7 43 6.55318453449857 7 44 5.864872174430044 7 45 5.254854179095485 7 46 4.370994952416002 7 47 4.46859111237633 7 48 5.035981028087843 7 49 8.65353567208076 7 50 4.844395999074585 7 51 7.535301520561198 7 52 6.958223866461481 7 53 7.005779171379018 7 54 9.102178182913857 7 55 7.120113588552837 7 56 7.603423714393333 8 1 2.147589660024636 8 2 1.6848221228590958 8 3 2.655624672340622 8 4 2.152300688017405 8 5 5.8111402777147845 8 6 6.674625864338918 8 7 2.2438303615101134 8 8 2.4302149204711228 8 9 2.757232614493063 8 10 3.631396257473483 8 11 4.003706995189191 8 12 5.388846278542282 8 13 4.744779362328693 8 14 6.127557186492552 8 15 5.837868992612989 8 16 5.305858104736394 8 17 5.025330366528877 8 18 5.81367686611204 8 19 4.276102961079744 8 20 4.249771436022989 8 21 3.328886821748071 8 22 3.956756643174799 8 23 2.1263632907053918 8 24 2.7898917041062745 8 25 3.3335080137226143 8 26 1.573510804984494 8 27 1.2273973919742147 8 28 1.751243040155579 8 29 2.0178638247144534 8 30 0.9122806235015872 8 31 1.5216240622480939 8 32 2.65593224855067 8 33 1.4117114882021937 8 34 1.877032293056672 8 35 1.0582768631365909 8 36 1.2816504705877634 8 37 2.175374839850271 8 38 2.125816737592473 8 39 2.0276974472537574 8 40 4.935928248823778 8 41 -5.6847480058068385 8 42 3.402098472441157 8 43 3.917219622217864 8 44 3.829784455131797 8 45 3.8710822492709234 8 46 1.6363458296089846 8 47 3.4269244457096626 8 48 2.038174010543983 8 49 6.500026900150939 8 50 2.2479047710044107 8 51 3.5967050293331226 8 52 3.58103088400534 8 53 4.96191952225621 8 54 4.988143095194559 8 55 4.617920606096696 8 56 4.522283363516138 9 1 1.014256326691303 9 2 2.72648878952576 9 3 2.155624672340622 9 4 1.8106340213507437 9 5 4.752806944381447 9 6 6.457959197672256 9 7 1.4438303615101162 9 8 2.146881587137784 9 9 2.4155659478264013 9 10 1.4813962574734845 9 11 2.9703736618558523 9 12 4.863846278542283 9 13 4.086446028995354 9 14 4.67755718649255 9 15 5.229535659279655 9 16 3.6308581047363973 9 17 2.9169970331955426 9 18 5.113676866112037 9 19 3.2177696277464065 9 20 3.7997714360229864 9 21 2.703886821748071 9 22 2.023423309841462 9 23 1.7846966240387303 9 24 2.648225037439609 9 25 1.4585080137226143 9 26 1.3901774716511568 9 27 0.8773973919742133 9 28 0.44290970682224184 9 29 0.7928638247144519 9 30 0.4539472901682515 9 31 0.004957395581428159 9 32 -0.2440677514493288 9 33 0.5367114882021937 9 34 -0.8563010402766622 9 35 -0.7750564701967448 9 36 0.8649838039210991 9 37 1.725374839850268 9 38 1.5174834042591385 9 39 2.0943641139204203 9 40 5.5359282488237795 9 41 5.081918660859831 9 42 3.78543180577449 9 43 6.800552955551197 9 44 5.9964511217984615 9 45 4.162748915937588 9 46 4.7946791629423196 9 47 5.96859111237633 9 48 4.946507343877318 9 49 8.816693566817602 9 50 5.331238104337743 9 51 6.7300383626664555 9 52 4.897697550672007 9 53 6.0452528555895455 9 54 6.35480976186123 9 55 5.459587272763361 9 56 5.8639500301828065 10 1 1.7879405372176151 10 2 2.8185940526836575 10 3 1.4187825670774643 10 4 2.952739284508638 10 5 4.852806944381449 10 6 6.744801302935411 10 7 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55 -8.423746060569968 118 56 -5.436049969817194 119 1 0.3142563266913001 119 2 -0.023511210474239874 119 3 0.35562467234062467 119 4 0.6439673546840723 119 5 3.2528069443814474 119 6 4.407959197672252 119 7 0.9104970281767777 119 8 0.8968815871377842 119 9 0.8155659478263999 119 10 0.948062924140153 119 11 -5.5129596714774785 119 12 -12.08615372145772 119 13 -13.396887304337977 119 14 -10.972442813507449 119 15 -7.720464340720348 119 16 -13.485808561930273 119 17 -15.883002966804458 119 18 -16.119656467221294 119 19 -7.84889703892026 119 20 -8.183561897310344 119 21 -3.046113178251929 119 22 0.30675664317479345 119 23 -0.8653033759612754 119 24 0.5982250374396116 119 25 0.1251746803892786 119 26 1.006844138317824 119 27 0.9940640586408804 119 28 0.9262430401555761 119 29 0.2761971580477862 119 30 1.0706139568349187 119 31 0.2716240622480939 119 32 -5.8274010847826645 119 33 -0.013288511797803437 119 34 -2.0729677069433308 119 35 0.4416101964699237 119 36 0.7483171372544319 119 37 1.8587081731836008 119 38 2.0174834042591385 119 39 1.1943641139204217 119 40 2.719261582157113 119 41 2.3985853275264972 119 42 -2.5479015275588424 119 43 -16.632780377782133 119 44 -9.503548878201538 119 45 -9.987251084062411 119 46 -19.68865417039102 119 47 -20.464742220957003 119 48 -17.28682598945602 119 49 -17.01663976651573 119 50 -15.685428562328925 119 51 -12.369961637333542 119 52 -12.118969115994659 119 53 -12.404747144410454 119 54 -14.045190238138773 119 55 -11.290412727236635 119 56 -8.119383303150528 120 1 0.5642563266913001 120 2 1.0598221228590958 120 3 1.3222913390072861 120 4 1.1939673546840766 120 5 4.102806944381449 120 6 5.091292531005589 120 7 1.2438303615101134 120 8 1.2635482538044513 120 9 1.1322326144930628 120 10 1.9647295908068187 120 11 -2.662959671477477 120 12 -11.736153721457718 120 13 -10.463553971004643 120 14 -8.622442813507448 120 15 -10.870464340720346 120 16 -16.569141895263606 120 17 -13.183002966804459 120 18 -14.519656467221292 120 19 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>>Per sequence quality scores pass #Quality Count 4 103.0 5 15.0 6 2.0 7 6.0 8 3.0 9 8.0 10 6.0 11 8.0 12 7.0 13 18.0 14 16.0 15 44.0 16 59.0 17 69.0 18 73.0 19 114.0 20 131.0 21 164.0 22 190.0 23 257.0 24 286.0 25 332.0 26 372.0 27 429.0 28 520.0 29 604.0 30 690.0 31 801.0 32 919.0 33 1044.0 34 1184.0 35 1390.0 36 1797.0 37 2278.0 38 1968.0 39 912.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.78596908442331 0.1961950059453032 0.011890606420927468 0.005945303210463734 8 99.68489892984542 0.2318668252080856 0.041617122473246136 0.041617122473246136 9 99.40546967895362 0.1724137931034483 0.11890606420927466 0.3032104637336504 10 53.12128418549346 31.623067776456597 8.02615933412604 7.2294887039239 11 30.79667063020214 30.291319857312722 20.85612366230678 18.05588585017836 12 32.8537455410226 14.803804994054698 26.200951248513675 26.141498216409037 13 22.493756689261506 16.369366155309788 29.17112617433702 31.965750981091688 14 23.22968071823533 20.53035257744218 28.164575777394614 28.07539092692788 15 23.64438088428078 20.247884638302942 28.137289953521634 27.97044452389465 16 28.456687291765824 21.138743455497384 27.21918134221799 23.185387910518802 17 26.819262782401903 23.061831153388823 24.44114149821641 25.677764565992867 18 25.564803804994057 22.193816884661118 24.79191438763377 27.44946492271106 19 24.936038555363837 24.222050336169453 25.98917117867555 24.85273992979116 20 25.76154212279867 22.191813422179916 27.153736316039982 24.892908138981436 21 25.184304399524375 21.89655172413793 27.841854934601663 25.077288941736033 22 26.20689655172414 20.998810939357906 28.995243757431627 23.799048751486325 23 26.730083234244944 21.7538644470868 27.907253269916765 23.608799048751486 24 25.334443189250255 24.733931862774245 26.18467209703312 23.74695285094239 25 25.57669441141498 23.686087990487515 28.210463733650414 22.526753864447084 26 23.228299643281808 21.95600475624257 32.241379310344826 22.574316290130795 27 24.717295560052374 16.932508034757767 35.781454588739436 22.568741816450423 28 21.894160366688492 19.43568069527948 36.781951306625395 21.88820763140663 29 22.713215177828 17.83037944570001 39.06268585702391 20.393719519448076 30 20.745629682483056 16.280176001902724 41.97883220359139 20.995362112022832 31 20.8955223880597 16.62008681691146 42.49866206814533 19.98572872688351 32 22.41204833621049 16.203345437228407 42.24656229537472 19.13804393118638 33 21.099333015721772 16.757979990471654 43.610052405907574 18.532634587899 34 18.960390151064587 16.159153086713456 47.29986915665517 17.580587605566787 35 19.38975792541486 16.035210848747994 48.62903705466009 15.945994171177066 36 19.041389153187442 17.673644148430068 47.36560418648906 15.919362511893434 37 20.135827475277015 16.817586083641128 46.038365304420346 17.008221136661504 38 19.574417357096024 17.303451153364726 47.261131310723016 15.861000178816237 39 19.988084599344653 17.295204051236222 46.85731307715222 15.859398272266906 40 15.36573686100802 13.079133245540525 40.47048964443912 31.08464024901233 41 13.673420453183072 12.984054669703873 40.01318786716221 33.32933700995084 42 17.689423248616155 16.772811142193916 48.038807213856316 17.49895839533361 43 15.276453765490944 15.079837940896091 47.0150142993327 22.628693994280265 44 15.425976819213766 15.58131198470546 45.136814434221534 23.855896761859245 45 18.524033593424267 16.826493537435226 47.97784263505867 16.67163023408184 46 18.051831992850758 17.438188859100386 46.94072088173965 17.569258266309205 47 17.002796121125588 17.30620500922125 47.33178654292343 18.35921232672973 48 17.750387042991544 18.29820173871621 47.42765273311897 16.523758485173275 49 15.268817204301074 15.46594982078853 42.03703703703704 27.228195937873355 50 16.669643388700365 18.62237304280526 47.6215990950765 17.086384473417873 51 17.60462620722547 18.814832478836294 47.57958745677835 16.000953857159892 52 16.277685163134077 20.016670635865683 45.94546320552513 17.760180995475114 53 17.518161248064786 20.16196260569251 45.24234845778254 17.077527688460165 54 15.734890928596974 18.57789963046847 43.56299916557397 22.124210275360593 55 17.123450905624406 19.61987607244995 45.996186844613916 17.260486177311726 56 17.127203430204858 20.35493091948547 45.13458789899952 17.383277751310146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 4.0 9 8.0 10 28.5 11 49.0 12 65.0 13 83.0 14 85.0 15 118.0 16 151.0 17 180.0 18 209.0 19 233.0 20 257.0 21 291.5 22 414.0 23 502.0 24 590.0 25 678.0 26 732.5 27 787.0 28 787.0 29 767.0 30 747.0 31 690.0 32 633.0 33 590.5 34 548.0 35 492.5 36 437.0 37 378.5 38 319.5 39 319.0 40 320.0 41 321.0 42 359.5 43 398.0 44 419.5 45 441.0 46 461.0 47 489.0 48 497.0 49 540.5 50 584.0 51 598.0 52 612.0 53 625.5 54 654.0 55 669.0 56 687.0 57 705.0 58 749.0 59 793.0 60 796.5 61 800.0 62 744.5 63 633.5 64 578.0 65 535.0 66 492.0 67 431.0 68 370.0 69 336.5 70 303.0 71 241.0 72 182.0 73 185.0 74 134.5 75 84.0 76 61.0 77 38.0 78 31.0 79 18.0 80 12.0 81 6.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.011890606420927468 14 0.005945303210463734 15 0.2259215219976219 16 0.0713436385255648 17 0.0 18 0.0 19 0.07728894173602853 20 0.0713436385255648 21 0.0 22 0.0 23 0.0 24 0.005945303210463734 25 0.0 26 0.0 27 0.1070154577883472 28 0.1248513674197384 29 0.0356718192627824 30 0.011890606420927468 31 0.0178359096313912 32 0.1248513674197384 33 0.16646848989298454 34 0.0356718192627824 35 0.041617122473246136 36 0.023781212841854936 37 0.20214030915576697 38 0.2556480380499405 39 0.2080856123662307 40 0.6777645659928656 41 0.8204518430439952 42 0.11296076099881094 43 0.2140309155766944 44 0.4875148632580262 45 0.18430439952437574 46 0.2080856123662307 47 0.06539833531510107 48 0.15457788347205706 49 0.47562425683709864 50 0.13674197384066586 51 0.2734839476813317 52 0.1426872770511296 53 0.15457788347205706 54 0.2497027348394768 55 0.2140309155766944 56 0.16646848989298454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 16820.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.08561236623068 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7976244860667 67.77645659928656 2 7.9107659509669555 12.354340071343639 3 2.36028627988427 5.529131985731272 4 1.111618699558398 3.4720570749108206 5 0.6624029237094564 2.586206896551724 6 0.3273945485000761 1.5338882282996433 7 0.22841480127912286 1.2485136741973841 8 0.12943505405816963 0.8085612366230679 9 0.11420740063956143 0.8026159334126041 >10 0.3578498553372925 3.8882282996432815 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGTAAGGGGTCTCTGATGTTGGGGTTAGTGCCTTAGAGGGTACTGGGAGTCCAGC 24 0.1426872770511296 No Hit TCGTAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 22 0.13079667063020214 No Hit TCGTAAGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.1248513674197384 No Hit TCGTAAGGGAGTCTCTTGTCTCAGGCTCTCTGCCTTGTTATCAGTCTTAAGCATAT 21 0.1248513674197384 No Hit TCGTAAGGGCACTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.11890606420927466 No Hit TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.11890606420927466 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 19 0.11296076099881094 No Hit TCGTAAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.1070154577883472 No Hit TCGTAAGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.1070154577883472 No Hit TCGTAAGGGGTTTCCTATTGGGCAAAGTTAGTGTAAAAGTGACTTTCCTTTTTTTT 18 0.1070154577883472 No Hit TCGTAAGGGTGGCACGCAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.10107015457788349 No Hit TCGTAAGGGGTTGTTGTTGTTTTGGTTTGGTTTGTTCTGTTCTGTTTTTTGAGATA 17 0.10107015457788349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.011890606420927468 0.0 0.0 0.0 0.0 7 0.011890606420927468 0.0 0.0 0.0 0.0 8 0.011890606420927468 0.0 0.0 0.0 0.0 9 0.011890606420927468 0.0 0.0 0.0 0.0 10 0.0178359096313912 0.0 0.0 0.0 0.0 11 0.0178359096313912 0.0 0.0 0.0 0.0 12 0.0178359096313912 0.0 0.0 0.0 0.0 13 0.0178359096313912 0.0 0.0 0.0 0.0 14 0.029726516052318668 0.0 0.0 0.0 0.0 15 0.029726516052318668 0.0 0.0 0.0 0.0 16 0.0356718192627824 0.0 0.0 0.0 0.0 17 0.0356718192627824 0.0 0.0 0.0 0.0 18 0.0356718192627824 0.0 0.0 0.0 0.0 19 0.0356718192627824 0.0 0.0 0.0 0.0 20 0.0356718192627824 0.0 0.0 0.0 0.0 21 0.0356718192627824 0.0 0.0 0.0 0.0 22 0.0356718192627824 0.0 0.0 0.0 0.0 23 0.0356718192627824 0.0 0.0 0.0 0.0 24 0.041617122473246136 0.0 0.0 0.0 0.0 25 0.041617122473246136 0.0 0.0 0.0 0.0 26 0.04756242568370987 0.0 0.0 0.0 0.0 27 0.04756242568370987 0.0 0.0 0.0 0.0 28 0.0535077288941736 0.0 0.0 0.0 0.0 29 0.0535077288941736 0.0 0.0 0.0 0.0 30 0.06539833531510107 0.0 0.0 0.0 0.0 31 0.0713436385255648 0.0 0.0 0.0 0.0 32 0.0713436385255648 0.0 0.0 0.0 0.0 33 0.0713436385255648 0.0 0.0 0.0 0.0 34 0.07728894173602854 0.0 0.0 0.0 0.0 35 0.07728894173602854 0.0 0.0 0.0 0.0 36 0.07728894173602854 0.0 0.0 0.0 0.0 37 0.07728894173602854 0.0 0.0 0.0 0.0 38 0.07728894173602854 0.0 0.0 0.0 0.0 39 0.07728894173602854 0.0 0.0 0.0 0.0 40 0.07728894173602854 0.0 0.0 0.0 0.0 41 0.07728894173602854 0.0 0.0 0.0 0.0 42 0.07728894173602854 0.0 0.0 0.0 0.0 43 0.07728894173602854 0.0 0.0 0.0 0.0 44 0.08323424494649227 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACCAA 15 0.008008172 50.433735 37 AACCAAA 15 0.008008172 50.433735 38 CCCCCGA 15 0.008008172 50.433735 40 GCCCCCG 15 0.008008172 50.433735 39 AGAGCCC 15 0.0081043765 50.282284 36 AAAACCA 15 0.0081043765 50.282284 36 AAAAACC 15 0.0081043765 50.282284 35 AATTCGT 15 0.008299362 49.982094 13 CCACGCA 15 0.008299362 49.982094 14 ATGGTAT 15 0.008299362 49.982094 14 GAATGTA 15 0.008299362 49.982094 9 GAGCATG 15 0.008299362 49.982094 9 ATTCGTG 15 0.008299362 49.982094 14 ACCCCCC 15 0.008299362 49.982094 11 GAATTCG 15 0.008299362 49.982094 12 TATCACG 15 0.008299362 49.982094 14 GATCACG 15 0.008299362 49.982094 11 CATGGTA 15 0.008299362 49.982094 13 AATGTAT 15 0.008299362 49.982094 10 AGATCAC 15 0.008299362 49.982094 10 >>END_MODULE