FastQCFastQC Report
Fri 17 Jun 2016
SRR1526192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60793
Sequences flagged as poor quality0
Sequence length56
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3880.6382313753228168No Hit
AATAAAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1450.23851430263352688No Hit
AATAAAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1410.23193459773329164No Hit
AATAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.2089056305824684No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1020.16778247495599824No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG960.15791291760564538No Hit
AATAAAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG950.15626799138058659No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG820.1348839504548221No Hit
AATAAAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG690.11349990952905763No Hit
AATAAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.1118549833039988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCA150.00809242750.462539
GCGAACC150.00809242750.462539
TACGCCC150.00809242750.462538
CGAATTG150.00809242750.462541
CGAACCT150.00809242750.462540
GGCCGCC203.9676134E-450.46249838
TACCCAC251.9542922E-550.46249838
CGGCGAA150.0082266950.25311737
CGGCCGC204.0499913E-450.2531137
CCCAGTT150.00825373950.21144542
TGTAGCG150.00825373950.21144542
ACTCAAC150.00833528850.08685334
TGCGCTG150.00838998450.0041311
GTCGCTA150.00838998450.0041313
CCGCCCG150.00838998450.0041315
ATGCGCT150.00838998450.0041310
CGCTAGG150.00838998450.0041315
TCGCTAG150.00838998450.0041314
ATGTTAA150.00838998450.0041310
AAGCCGT150.00838998450.0041310