FastQCFastQC Report
Fri 17 Jun 2016
SRR1526191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143301
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6900.4815039671739904No Hit
ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2940.20516255992630897No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2900.20237123258037276No Hit
ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2860.1995799052344366No Hit
ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2170.15142950851703757No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2050.14305552647922903No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1960.13677503995087265No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.12770322607658008No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1640.11444442118338323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT150.00810448250.47910741
TATTTGC203.9865775E-450.4612440
CCCATGA150.00811583750.46123540
AGCGTAG204.0075247E-450.40770737
TAAGCCC150.00819565550.3365136
GTAAGCC150.00821856850.30098735
TAAGACA150.00821856850.30098735
TCCATAC150.00828759150.19471744
CGATTCA150.00828759150.19471749
CGATTAA204.0921895E-450.19471749
ACTATTA301.0162694E-650.17704847
CTCACTA150.008299136550.17704845
ACGATTA252.0394073E-550.15939348
TCGACCA150.00831069350.15939348
TGTAGCG150.00832226350.1417542
CACCATA150.0083802950.0537233
CATATAC150.008473759549.91351328
ATATACT150.008473759549.91351329
CACCGTA204.2072803E-449.91351328
CCACCGT204.2072803E-449.91351327