##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526191_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 143301 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.40777803364945 39.0 38.0 40.0 33.0 40.0 2 36.53627678801962 39.0 38.0 40.0 30.0 40.0 3 36.774021116391374 39.0 38.0 40.0 31.0 40.0 4 36.30175644273243 39.0 37.0 40.0 29.0 40.0 5 36.53867035121876 39.0 38.0 40.0 30.0 40.0 6 36.26119845639598 39.0 37.0 40.0 30.0 40.0 7 36.064088875862694 39.0 36.0 40.0 29.0 40.0 8 36.10625187542306 39.0 36.0 40.0 29.0 40.0 9 36.11814292991675 39.0 36.0 40.0 29.0 40.0 10 35.64686917746561 39.0 35.0 39.0 27.0 40.0 11 34.9499654573241 38.0 34.0 39.0 26.0 40.0 12 32.843713581901035 38.0 30.0 39.0 17.0 40.0 13 32.637804341909685 37.0 30.0 39.0 16.0 40.0 14 32.863092372000196 38.0 30.0 39.0 17.0 40.0 15 32.86552780510952 38.0 30.0 39.0 17.0 40.0 16 33.08850601182127 38.0 31.0 39.0 18.0 40.0 17 33.19556737217465 37.0 31.0 39.0 20.0 40.0 18 32.67272384700735 37.0 30.0 39.0 17.0 40.0 19 33.53233403814349 37.0 31.0 39.0 23.0 40.0 20 33.3929421288058 37.0 31.0 39.0 22.0 40.0 21 34.26020753518817 38.0 33.0 39.0 23.0 40.0 22 33.99742500052337 38.0 33.0 39.0 23.0 40.0 23 34.27888151513248 38.0 33.0 39.0 24.0 40.0 24 33.96593882806121 38.0 33.0 39.0 23.0 40.0 25 34.10006908535181 38.0 33.0 39.0 23.0 40.0 26 34.65722500191904 38.0 34.0 39.0 26.0 40.0 27 34.543024821878426 38.0 34.0 39.0 25.0 40.0 28 34.469717587455776 38.0 34.0 39.0 25.0 40.0 29 34.33248895681119 38.0 33.0 39.0 25.0 40.0 30 34.13364177500506 38.0 33.0 39.0 23.0 40.0 31 34.38829456877482 38.0 34.0 39.0 26.0 40.0 32 33.985296683205284 38.0 33.0 39.0 23.0 40.0 33 34.16009657992617 38.0 33.0 39.0 25.0 40.0 34 33.91365028855346 38.0 33.0 39.0 23.0 40.0 35 33.84844488175239 38.0 33.0 39.0 23.0 40.0 36 33.08454232699004 36.0 32.0 39.0 23.0 40.0 37 32.858444811969214 36.0 32.0 39.0 22.0 40.0 38 32.587169663854404 36.0 32.0 38.0 20.0 40.0 39 32.35368908800357 36.0 31.0 38.0 17.0 40.0 40 29.120459731613877 35.0 23.0 38.0 5.0 39.0 41 25.96391511573541 31.0 14.0 38.0 4.0 39.0 42 29.745333249593514 34.0 28.0 38.0 4.0 39.0 43 28.228623666268902 33.0 24.0 38.0 4.0 39.0 44 28.23388531831599 33.0 24.0 38.0 4.0 39.0 45 29.284101297269384 33.0 27.0 38.0 4.0 39.0 46 28.836302607797574 34.0 26.0 38.0 4.0 40.0 47 28.37685012665648 34.0 24.0 38.0 4.0 40.0 48 28.443186021032652 33.0 25.0 38.0 4.0 39.0 49 25.95893957474128 32.0 11.0 38.0 4.0 39.0 50 28.088296662270327 33.0 24.0 38.0 4.0 39.0 51 28.31049329732521 35.0 24.0 38.0 4.0 40.0 52 28.033670386110355 34.0 23.0 38.0 4.0 40.0 53 27.659388280612138 34.0 20.0 38.0 4.0 40.0 54 26.739283047571195 33.0 11.0 38.0 4.0 40.0 55 27.07087180131332 33.0 16.0 38.0 4.0 40.0 56 26.302768298895334 33.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.3478667671668134 1 2 2.6485124837754555 1 3 2.2846341294548864 1 4 2.787275484047683 1 5 2.6609345095198833 1 6 2.961769357002943 1 7 2.9157300325458806 1 8 3.0147973555775636 1 9 2.960370837767371 1 10 3.03546157217977 1 11 -0.5154815337077423 1 12 -10.002682654897871 1 13 -5.614571541427306 1 14 -3.121236115144498 1 15 -4.102773911040064 1 16 -22.041796610948904 1 17 -4.064398286731862 1 18 -12.610023643056618 1 19 -4.5450362401072155 1 20 -4.355401378696275 1 21 -5.1110244031183925 1 22 -2.0211784113718423 1 23 -2.173541208139838 1 24 -2.5284220238502613 1 25 -1.7646613458120868 1 26 3.483033482440675 1 27 3.532197488705485 1 28 3.7475797047265544 1 29 3.4858831255334337 1 30 3.9093220857810635 1 31 3.159656507796157 1 32 3.5010329811965306 1 33 3.1877159475319914 1 34 3.2161269250416993 1 35 3.4785587714072292 1 36 3.8894736581042295 1 37 3.9966983057424876 1 38 3.6154708452468682 1 39 4.0633495389446885 1 40 6.672504820330047 1 41 -7.967749009773957 1 42 -7.481165614216067 1 43 -15.247117090371967 1 44 -20.808373279320282 1 45 -9.655460534778484 1 46 -17.31905139091692 1 47 -21.892863211245313 1 48 -14.305262984378649 1 49 -18.960057856910268 1 50 -13.885984765389036 1 51 -10.824737282986167 1 52 -10.577920362286722 1 53 -10.327549062555317 1 54 -9.991685262040143 1 55 -10.180808349448085 1 56 -6.3320820023071605 2 1 2.10239139488732 2 2 3.3018343623550663 2 3 3.019685675846638 2 4 3.5485584164646298 2 5 3.375310225441986 2 6 3.5672103650212676 2 7 3.7663027702320235 2 8 3.690799646528312 2 9 3.686774045098417 2 10 3.504018273210697 2 11 3.059146186796262 2 12 0.08769535197497902 2 13 1.320823647576134 2 14 4.890676828727205 2 15 4.828669387929011 2 16 3.4463477189480045 2 17 4.104158414299064 2 18 -0.08138675874963042 2 19 3.2254677690565856 2 20 3.644312252460658 2 21 3.7041531455643124 2 22 3.6958891716751197 2 23 3.569929582238167 2 24 2.723582558051227 2 25 3.3894050917595067 2 26 3.13755811016118 2 27 3.5681654153950575 2 28 3.7921959704768398 2 29 3.5217365046857765 2 30 3.7764469425966425 2 31 2.7606301618625935 2 32 3.7793262228918323 2 33 2.9387468753670376 2 34 3.2634923316854554 2 35 3.3132666751873003 2 36 3.9695423866265713 2 37 4.255747561183494 2 38 3.817589974685582 2 39 3.961173135737358 2 40 6.315803789402217 2 41 -4.086362984573501 2 42 -5.075552784891897 2 43 -11.409946414541498 2 44 -12.434719212882712 2 45 -3.755002344629574 2 46 -8.931250703631697 2 47 -16.893321401394225 2 48 -6.792261838903276 2 49 -10.856449609487589 2 50 -6.345664032284798 2 51 -1.6080706163195018 2 52 -1.6978516337643867 2 53 -1.6805272985232413 2 54 -0.4294286755567498 2 55 -1.3066960928615998 2 56 1.7883074593194124 3 1 2.053422322722369 3 2 3.372016757279731 3 3 3.0644418217625784 3 4 3.697745569517764 3 5 3.4214045949899656 3 6 3.4613420065755918 3 7 3.3869907163065633 3 8 3.5258016290818404 3 9 3.925969128365665 3 10 3.7364231106413115 3 11 4.056420175693965 3 12 6.016868627153407 3 13 5.356261791906029 3 14 5.285601491693111 3 15 4.778956858190703 3 16 5.282241850589557 3 17 5.418294020960445 3 18 5.7703182372852595 3 19 4.598980853909879 3 20 5.211372125577228 3 21 4.255749101155111 3 22 4.4798899646965324 3 23 4.612356227757594 3 24 4.734932677004437 3 25 4.662154893504152 3 26 3.838589037996229 3 27 3.8877530442610393 3 28 3.8981138927607475 3 29 3.5143019289522357 3 30 3.8056896071485866 3 31 3.112434285573933 3 32 3.496118451282001 3 33 3.4636774859935286 3 34 2.5304431643579335 3 35 2.828879284227746 3 36 4.168640324770898 3 37 4.056847878392059 3 38 4.237693067469088 3 39 4.507366632961787 3 40 8.156158666483897 3 41 9.17156722954228 3 42 5.727381394330944 3 43 0.23760513185025545 3 44 3.840558344611342 3 45 5.65404801223006 3 46 3.8322306603651306 3 47 -0.5523717582538623 3 48 3.7430276139119485 3 49 5.723595989243577 3 50 3.569036602132332 3 51 4.5890447682958815 3 52 4.977207842841484 3 53 4.432279997273746 3 54 5.417716447361567 3 55 4.324747206107471 3 56 4.469413724188566 4 1 1.6162428355428844 4 2 3.22586291112588 4 3 3.066685411506164 4 4 3.411207107979301 4 5 3.227173825759195 4 6 3.2818548270884094 4 7 3.531541998357845 4 8 3.5844554752356856 4 9 3.549687077083611 4 10 3.6059743926925947 4 11 4.351612483386276 4 12 6.648599396384178 4 13 6.434146407290648 4 14 5.995857901949517 4 15 5.877995319729166 4 16 5.562049542897249 4 17 5.477909405575829 4 18 6.131856698823725 4 19 4.5502629051919286 4 20 4.486051612756718 4 21 3.745813203719216 4 22 3.7651463749529412 4 23 3.837997253398619 4 24 4.423714728286491 4 25 3.8182446370938976 4 26 3.1591018585090467 4 27 3.1057017622097547 4 28 3.580485687632539 4 29 3.51750705715736 4 30 3.581971658430632 4 31 3.0156394137790627 4 32 3.344195374358925 4 33 2.9787415885576323 4 34 2.8926226515374225 4 35 2.8708664637149255 4 36 4.209986478617054 4 37 4.557809416853598 4 38 4.421026400802418 4 39 4.811212786807939 4 40 7.480197128022361 4 41 10.688233896208946 4 42 7.199176266125814 4 43 8.704592311337432 4 44 8.197609626662622 4 45 7.1659069865890395 4 46 6.900179378313851 4 47 6.2165384981563925 4 48 6.496873767758103 4 49 9.389942143089733 4 50 6.578972499568231 4 51 5.97398066573178 4 52 6.091310406944043 4 53 5.807921022914773 4 54 6.303613883259004 4 55 5.997824129184394 4 56 5.917490647265488 5 1 2.2076776418713067 5 2 3.295912010962219 5 3 3.216549023071906 5 4 3.520372410761631 5 5 3.4780739894253188 5 6 3.4813911064119267 5 7 3.551782042002145 5 8 3.4536862444664536 5 9 3.561198260935221 5 10 3.417376465796792 5 11 4.334754872911638 5 12 6.1022818841092175 5 13 6.195357536586883 5 14 6.523326532609641 5 15 6.7770133230024925 5 16 5.3645863677745 5 17 5.7781549047575 5 18 5.852151297841729 5 19 5.0339235707674845 5 20 5.315402403809635 5 21 4.41297086874922 5 22 4.717001257658893 5 23 4.4495084372502305 5 24 4.552547243289219 5 25 4.130137708561435 5 26 3.7162486124643124 5 27 3.5207317676652963 5 28 3.7693291137100076 5 29 3.36188785366582 5 30 3.758021849374444 5 31 2.982115136637759 5 32 3.487321397272183 5 33 3.3228223305107107 5 34 2.981138745372668 5 35 3.0984169274356006 5 36 4.271033941792176 5 37 4.024830693449342 5 38 4.546203705766963 5 39 4.660394456202372 5 40 8.627823969266224 5 41 10.156837278642119 5 42 7.470016418880856 5 43 8.313403004190679 5 44 8.220468328244731 5 45 6.8967853281056755 5 46 6.559317403409324 5 47 6.6357419897003105 5 48 6.706202736661538 5 49 9.083559164366328 5 50 6.894629891821367 5 51 5.875971936871984 5 52 5.944065453316117 5 53 6.678597509548705 5 54 7.130891570111157 5 55 6.143896142277686 5 56 5.792858896037991 6 1 2.2760999183507877 6 2 3.4163631212982253 6 3 3.2283499764494223 6 4 3.6776215678363897 6 5 3.565215019538094 6 6 3.589547134780716 6 7 3.813088866789961 6 8 3.718713566870825 6 9 3.7744874133593598 6 10 3.7297238672617397 6 11 4.407013912558192 6 12 6.813710787725078 6 13 6.517122447643736 6 14 6.685139112542206 6 15 6.578373629943542 6 16 6.330607760635793 6 17 5.952814828022234 6 18 6.337153041824983 6 19 4.855601282661453 6 20 5.54305035172267 6 21 4.472673228939897 6 22 4.77481430265366 6 23 4.564436933936662 6 24 5.019847557206205 6 25 4.5158486724028535 6 26 3.9560753767940398 6 27 3.857698399452296 6 28 3.8474886300453193 6 29 4.653278389650005 6 30 4.072345765198186 6 31 3.5420790943171028 6 32 3.972526605968845 6 33 3.3060219584609527 6 34 3.408385577136414 6 35 3.640853853374445 6 36 4.211331581601499 6 37 4.338137285706058 6 38 4.061463559272369 6 39 3.910161925101338 6 40 7.290537607215299 6 41 3.6813402434136577 6 42 5.649617628042588 6 43 7.433301853161733 6 44 6.7437214383482065 6 45 5.641989374146835 6 46 5.809637133673242 6 47 6.480178683108054 6 48 6.097378181377273 6 49 8.277373837078798 6 50 6.286328531514428 6 51 6.509415722478309 6 52 6.276177998369011 6 53 6.627004671506615 6 54 7.31168450116569 6 55 6.293326441080151 6 56 6.071925283667337 7 1 1.910163895756078 7 2 3.290772159181195 7 3 3.134072331702079 7 4 3.534716183173771 7 5 3.2864650962864204 7 6 3.5801838389005667 7 7 3.3306488839815884 7 8 3.2739109635675803 7 9 3.145826518166878 7 10 3.49782112274157 7 11 4.2810852578028715 7 12 6.581961539858682 7 13 6.379435949209402 7 14 6.507727680111433 7 15 6.40790024626331 7 16 5.609326984556716 7 17 5.5642533986614 7 18 5.883109939964605 7 19 4.802092349156204 7 20 4.822813352889241 7 21 3.6874150475682512 7 22 4.088684260288581 7 23 3.972338941672895 7 24 4.344861371905047 7 25 3.889520926347714 7 26 3.355442970580498 7 27 3.5001125948228378 7 28 2.97498295066913 7 29 3.702612214172632 7 30 3.71331709202326 7 31 2.751479229394157 7 32 3.6142040174012706 7 33 3.0691766216892944 7 34 3.202331669111608 7 35 3.269944539197496 7 36 3.5359156056697714 7 37 3.882528517977196 7 38 3.437131269716282 7 39 3.8087927349496873 7 40 6.074752011341289 7 41 -6.453892995494925E-4 7 42 4.305600927606651 7 43 5.897015244209808 7 44 5.93120225126648 7 45 3.7043397149094055 7 46 4.899862466760986 7 47 4.90576350535893 7 48 4.959967976919728 7 49 7.28544776106726 7 50 4.621381027370017 7 51 4.948109158961394 7 52 4.6438024841552235 7 53 4.274760550428454 7 54 5.676791641830022 7 55 4.9230618128490455 7 56 4.92284933352305 8 1 2.2582245826093725 8 2 3.3066104122123647 8 3 3.303278197251494 8 4 3.680307499113596 8 5 3.488712287297659 8 6 3.600846569808965 8 7 3.6433194864065186 8 8 3.501708843336509 8 9 3.5197870336242474 8 10 3.85438812585209 8 11 4.378209615068151 8 12 6.848164802729244 8 13 6.254919983996423 8 14 6.649573677699191 8 15 6.345531173705702 8 16 6.03645197662172 8 17 5.674867249991301 8 18 6.054759784438673 8 19 5.283683157256249 8 20 5.218515758780185 8 21 4.285491604414567 8 22 4.74341669220631 8 23 3.984368396379935 8 24 4.650485697430341 8 25 4.369048635355526 8 26 4.073052314832388 8 27 4.009221970814714 8 28 3.9945665220523594 8 29 3.764790846814037 8 30 3.452636586722683 8 31 2.7360218561954 8 32 3.2112966346805223 8 33 3.150145326063061 8 34 2.6953171321980065 8 35 2.5932480369439546 8 36 3.2346713982172233 8 37 3.434645575045689 8 38 3.3916478697289776 8 39 3.905345772466351 8 40 5.784934198861123 8 41 0.3781078640490172 8 42 4.298872050567358 8 43 5.6878169962570375 8 44 5.479573989982921 8 45 4.52853193226483 8 46 5.807784767275173 8 47 5.444613248265046 8 48 5.566582590009297 8 49 7.788812199586907 8 50 5.303092447416994 8 51 6.163115824358464 8 52 5.5446785077697776 8 53 5.403053573979523 8 54 6.672062383910895 8 55 5.323334776728942 8 56 4.3745093347926485 9 1 2.0210693815458995 9 2 3.4403425491349324 9 3 3.3140458941607704 9 4 3.700347379472518 9 5 3.5298887578858924 9 6 3.281703997525817 9 7 3.44677578417987 9 8 3.6654509503488057 9 9 3.590109399858875 9 10 3.9795792192385946 9 11 4.531250492436044 9 12 6.534572247062911 9 13 6.524644144847208 9 14 6.683339048254194 9 15 6.700167265430522 9 16 6.178791624345216 9 17 5.5973664191504895 9 18 6.224616879819202 9 19 5.013460491918927 9 20 5.230546333722025 9 21 4.339629191652854 9 22 4.936337928497437 9 23 4.178419576173887 9 24 4.972885165692226 9 25 4.177822314318632 9 26 3.9091772732903465 9 27 3.852458926613977 9 28 3.8381025805435485 9 29 4.414641295468073 9 30 4.171413589049038 9 31 3.816846050279814 9 32 4.064104876621364 9 33 3.608304182826103 9 34 3.4733164675253576 9 35 3.9393430851327196 9 36 4.379669736535604 9 37 4.234280005088895 9 38 4.905340126292614 9 39 4.64831685920613 9 40 7.20779893797711 9 41 3.589440532709702 9 42 6.302494516502886 9 43 7.554516896556141 9 44 7.275286851398672 9 45 5.83408194888165 9 46 5.632909393396808 9 47 6.058770775682792 9 48 4.918593224771676 9 49 8.313471554854438 9 50 5.976106737878936 9 51 5.315458795445203 9 52 5.847569833791706 9 53 5.813954205418543 9 54 7.107987940574237 9 55 6.457100147283942 9 56 5.7143232264510075 10 1 2.0564343709151416 10 2 3.348847155796875 10 3 2.907525695719947 10 4 3.3011978401387125 10 5 3.1031701186229625 10 6 2.904808178957424 10 7 3.5361450258524414 10 8 3.1826019071170535 10 9 3.3102369689588045 10 10 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3.641325509956083 115 37 3.4911427501869348 115 38 4.309915289691311 115 39 4.392979168574325 115 40 7.3799122277374565 115 41 6.3748435828186345 115 42 2.6299454968950435 115 43 -5.052672645927522 115 44 -6.249114020061022 115 45 1.8149098355918873 115 46 -2.6616439835095136 115 47 -2.1891595075416106 115 48 -2.4774852066008712 115 49 -12.684131930984343 115 50 -2.693392172796443 115 51 -1.478440986689872 115 52 -0.8631055474719069 115 53 -1.3442157292219825 115 54 -6.5231667435216245 115 55 -0.9622898309295671 115 56 0.9049550347298769 116 1 1.580530756457307 116 2 2.8187266738691648 116 3 2.5762003945151264 116 4 3.006017117247147 116 5 2.681483371635011 116 6 3.145772034379114 116 7 3.102410086093407 116 8 3.1886260035025984 116 9 3.1295140773925425 116 10 3.397509764950854 116 11 0.5057366724502188 116 12 -11.692642494255303 116 13 -6.30412977435903 116 14 -0.7737126881030001 116 15 -4.998557043570184 116 16 -2.3893869723946857 116 17 -1.050542865045113 116 18 -5.7699968692949035 116 19 -1.5539385158769612 116 20 -2.1261510440242546 116 21 3.0447319556500148 116 22 4.074537786753993 116 23 3.5780652175629726 116 24 3.544536476819083 116 25 3.3564899259415952 116 26 3.4048540982371946 116 27 3.0142590683574255 116 28 2.617124550777426 116 29 3.3295243571264734 116 30 3.2091882169724926 116 31 2.306711394007671 116 32 2.874593891504425 116 33 2.526872574038009 116 34 2.5764348233014047 116 35 2.7560688115678786 116 36 4.205537915132346 116 37 4.169857609624682 116 38 4.319018368673909 116 39 4.309266540283378 116 40 7.625516868522823 116 41 5.417860093305023 116 42 0.9002266213930348 116 43 -4.6712804103184205 116 44 -6.076378634072626 116 45 1.2319557972161626 116 46 -3.4409389411177216 116 47 -1.6513638805893578 116 48 -2.6698546845125186 116 49 -7.557045808717497 116 50 -2.0174439354024223 116 51 -1.0779501343917914 116 52 -0.5100488763429496 116 53 -1.7885263048578608 116 54 -6.4620333209424174 116 55 -0.0035660469045772913 116 56 0.9669809160328668 117 1 0.740397112638334 117 2 1.6218551541769486 117 3 1.4955584992027866 117 4 2.050767547539742 117 5 1.7912332957010193 117 6 2.0321241655930464 117 7 2.0467757841798715 117 8 2.172173639424436 117 9 2.064058979690813 117 10 2.494705269658766 117 11 -1.321690684034543 117 12 -9.393158845374064 117 13 -9.572834846749434 117 14 -9.767081119813032 117 15 -8.40991676818292 117 16 -16.025410056327054 117 17 -13.484986522025977 117 18 -15.288828498332062 117 19 -5.884858835812164 117 20 -6.477016691488057 117 21 0.2900493597200793 117 22 0.7472622982453387 117 23 0.02799940810665902 117 24 -0.0027450864086162596 117 25 0.36605760843627877 117 26 1.7251436598449672 117 27 2.11884548123583 117 28 2.2733966981906093 117 29 1.7138009593336179 117 30 2.7361194714019774 117 31 1.8403754620445198 117 32 2.701920002671784 117 33 1.5192285525740061 117 34 2.338862686012753 117 35 2.3225363624436497 117 36 2.940173938216276 117 37 3.359490089122506 117 38 2.5297098741917807 117 39 3.0466361869372243 117 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118 23 2.8273774270307612 118 24 2.7139226830613765 118 25 2.9579983217294696 118 26 3.456699274216703 118 27 3.4113750915051355 118 28 3.170108138672312 118 29 3.79935644136895 118 30 3.4364436955798396 118 31 2.3299539706133032 118 32 -1.7798506583485256 118 33 2.572361616823329 118 34 2.5659519469139624 118 35 2.4231781939794104 118 36 3.6120458365819204 118 37 4.071195243625251 118 38 4.062496217075385 118 39 4.065361787413632 118 40 7.931559938440287 118 41 4.353729555405394 118 42 -0.5348838994304153 118 43 -7.641824002008011 118 44 -9.075477391306283 118 45 -1.5871315758983435 118 46 -10.306321732124267 118 47 -11.344656737054589 118 48 -11.284834287965701 118 49 -12.747065730926018 118 50 -9.120280478424906 118 51 -7.7616139234061166 118 52 -7.441174955462575 118 53 -7.102395956693549 118 54 -13.619987974201122 118 55 -7.500055943498829 118 56 -5.59577404080235 119 1 0.6145334338334791 119 2 1.8577717430347178 119 3 1.6290785738993279 119 4 2.024312521084717 119 5 1.903527102112477 119 6 2.248806394039974 119 7 2.0620263288421796 119 8 2.0129455037257102 119 9 2.014074541471075 119 10 2.09657268329088 119 11 -3.8432593114855216 119 12 -10.765645617860837 119 13 -11.744201171056933 119 14 -10.01382870773709 119 15 -9.412033170299324 119 16 -12.91772253687483 119 17 -15.295879768213343 119 18 -15.528542161575137 119 19 -7.439480684551661 119 20 -8.253549526844424 119 21 -0.572135514229501 119 22 1.3417845515911182 119 23 0.9171995326009039 119 24 0.753059457631224 119 25 1.1390423578916185 119 26 2.0793297787369696 119 27 1.9507160072240026 119 28 2.0420241491709987 119 29 2.511809051459359 119 30 2.4389517154106883 119 31 1.1911379892776353 119 32 -3.1230410928775427 119 33 2.3525307623468024 119 34 1.3883491472639164 119 35 1.800780993629452 119 36 3.1079921766227514 119 37 2.742994602038781 119 38 2.5247301045061263 119 39 2.963349538944694 119 40 6.816949264774493 119 41 0.5822509902260435 119 42 -2.8737582068086596 119 43 -11.311931905186782 119 44 -10.056521427468432 119 45 -7.251756831074779 119 46 -15.987569909435438 119 47 -12.944715063097167 119 48 -13.73674446586013 119 49 -13.232280079132492 119 50 -12.330429209833481 119 51 -11.204366912615798 119 52 -10.603846288212647 119 53 -11.49236387737013 119 54 -13.78983341018829 119 55 -10.20673427537401 119 56 -9.102452372677531 120 1 0.6234223227223694 120 2 1.8574013726643415 120 3 1.5246341294548884 120 4 2.0450532618254584 120 5 1.7987122872976613 120 6 2.1828804681140497 120 7 2.137952254768102 120 8 2.1236862444664553 120 9 2.0448152822118217 120 10 2.2154615721797697 120 11 -1.5599259781521866 120 12 -11.196015988231206 120 13 -9.041238208093972 120 14 -11.589013892922274 120 15 -9.102773911040064 120 16 -15.491796610948903 120 17 -13.55439828673186 120 18 -15.238912531945505 120 19 -6.939480684551661 120 20 -13.236512489807385 120 21 -3.842135514229504 120 22 -2.628956189149619 120 23 -1.8568745414731715 120 24 -7.150644246072481 120 25 -1.2268835680343066 120 26 1.838589037996229 120 27 1.7277530442610427 120 28 1.8286908158376676 120 29 1.4069942366445432 120 30 1.5837665302255033 120 31 -0.08256571442606742 120 32 -12.211189241025693 120 33 0.6377159475319871 120 34 0.9539047028194716 120 35 0.8552254380739015 120 36 1.2361403247709006 120 37 1.9678094168536013 120 38 1.9976930674690863 120 39 2.16890509450025 120 40 5.212504820330054 120 41 2.8768003819124033E-5 120 42 -4.093387836438289 120 43 -10.624894868149745 120 44 -8.260595501542504 120 45 -8.567682757000707 120 46 -14.732384724250252 120 47 -13.810640989023092 120 48 -11.500818539934205 120 49 -17.16005785691027 120 50 -11.441540320944592 120 51 -8.878070616319501 120 52 -9.251253695620058 120 53 -10.073104618110872 120 54 -12.676129706484586 120 55 -8.985252793892528 120 56 -6.45097089119605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3106.0 5 237.0 6 667.0 7 151.0 8 226.0 9 237.0 10 216.0 11 250.0 12 243.0 13 295.0 14 345.0 15 447.0 16 590.0 17 710.0 18 871.0 19 1035.0 20 1234.0 21 1431.0 22 1856.0 23 2205.0 24 2665.0 25 2743.0 26 3014.0 27 3453.0 28 3996.0 29 4533.0 30 5279.0 31 6276.0 32 6992.0 33 7888.0 34 9307.0 35 11042.0 36 13937.0 37 17649.0 38 17446.0 39 10721.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.50384156425984 0.41241861536207003 0.04815039671739904 0.035589423660686244 8 99.13468852275979 0.7110906413772409 0.08583331588753743 0.06838751997543632 9 97.45221596499675 1.601524064730881 0.4291665794376871 0.5170933908346766 10 49.700979058066586 33.59502027201485 8.875025296404072 7.82897537351449 11 34.24609737545447 29.863713442334667 18.997076084605133 16.893113097605738 12 36.55312942687071 14.592361532717845 23.22803050920789 25.62647853120355 13 25.039438232050372 17.80793231980567 24.843294104507823 32.309335343636135 14 24.007983586193422 18.587658938964648 30.59025500020936 26.814102474632573 15 26.98183955004617 18.761367770098218 28.85244984190055 25.404342837955063 16 30.72991579505942 20.019968999176104 25.16861934618983 24.081495859574648 17 29.355691865374283 21.428322202915542 24.255936804348888 24.960049127361287 18 27.747770380605452 24.387639743750785 24.985694147859704 22.87889572778406 19 28.761228852037608 26.60906131686668 21.553109152125625 23.076600678970088 20 28.88783342995439 26.59896208030956 21.817267463382947 22.695937026353104 21 27.828835807147197 23.58532040948772 26.495976999462666 22.089866783902416 22 25.80093649032456 23.212678208805244 26.99981158540415 23.986573715466047 23 27.456193606464712 23.224541350025472 25.948876839659178 23.370388203850638 24 26.883648874032616 23.216491392124162 27.139756732426605 22.760103001416617 25 27.478785171951763 23.55613555158553 25.32240955783832 23.642669718624386 26 24.174221328922666 21.016965712650656 29.760135111557762 25.048677846868916 27 22.61525565800503 20.292679519418833 30.666387259010897 26.42567756356524 28 22.162906234933235 24.135473367199346 30.25231809828597 23.44930229958145 29 25.784113199907853 22.540156787736212 30.53591248926709 21.139817523088844 30 23.215706528566844 19.75250910816734 36.249110121581815 20.782674241684 31 23.98958522386184 21.20649457621913 35.23712462829301 19.566795571626017 32 27.539966895048924 19.039537368784963 34.95505688603935 18.465438850126763 33 25.425683610132527 19.50455740088352 35.978303416652686 19.091455572331263 34 20.41497368016867 21.226210921682796 41.10431589382706 17.25449950432148 35 20.44123293887667 22.52382448423919 41.737005620134745 15.297936956749398 36 21.039882161581044 24.77468987134111 38.176017647839046 16.0094103192388 37 22.26277117109559 22.657074742898693 38.59001517089984 16.49013891510588 38 22.981483814082377 22.682677639221286 37.81262683517376 16.52321171152258 39 21.94270767767474 22.060201698068344 37.03579371406991 18.961296910187013 40 16.933073607077308 15.68765339202959 34.3352015808609 33.0440714200322 41 14.060705958594847 15.738607947141157 32.77051062600819 37.43017546825581 42 19.339197507143904 20.2963759964787 38.352814593827944 22.01161190254945 43 16.181232036587666 17.528793907649703 39.42475122203652 26.86522283372611 44 16.84831397632273 17.163216069124164 37.56803007350054 28.420439881052573 45 20.03594330188877 20.9002608336888 39.82783577966896 19.23596008475347 46 19.174816668647367 22.06897033842024 38.206323795675544 20.549889197256846 47 17.38025592873648 21.648527327687912 40.18695503445194 20.78426170912366 48 18.43824912812843 21.797837618724795 41.57796523696037 18.18594801618641 49 16.547181458514547 17.439462386711188 35.01851903813238 30.994837116641882 50 19.222545210610168 20.536937138384996 40.41632892640523 19.824188724599605 51 18.6653507758856 22.361743975250054 40.18366475582526 18.789240493039078 52 17.431128562345855 21.45826492185371 41.12653620160834 19.984070314192092 53 18.699499538680907 20.492353286549054 42.04434255039561 18.763804624374423 54 16.05553030038116 20.94485435535196 39.5139350281498 23.485680316117076 55 18.216411698458998 22.053176085451074 38.7873446232836 20.94306759280633 56 20.17854770943003 21.58651594275847 37.3167509070699 20.918185440741595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 2.5 4 15.0 5 25.0 6 31.0 7 37.0 8 44.0 9 51.0 10 133.5 11 216.0 12 351.0 13 610.5 14 735.0 15 913.5 16 1092.0 17 1331.5 18 1571.0 19 1638.0 20 1705.0 21 1897.5 22 2526.0 23 2962.0 24 3490.5 25 4019.0 26 4442.5 27 4866.0 28 5255.5 29 5476.0 30 5307.0 31 4957.5 32 4608.0 33 4157.0 34 3706.0 35 3475.0 36 3244.0 37 3077.0 38 2834.5 39 2759.0 40 2873.5 41 2988.0 42 3156.5 43 3325.0 44 3914.0 45 4503.0 46 4712.0 47 4855.0 48 4789.0 49 4786.5 50 4784.0 51 5119.0 52 5454.0 53 5681.5 54 6251.0 55 6593.0 56 7054.5 57 7516.0 58 8481.5 59 9447.0 60 9264.0 61 9081.0 62 8061.0 63 6330.5 64 5620.0 65 4918.0 66 4216.0 67 3714.0 68 3212.0 69 2695.5 70 2179.0 71 1840.5 72 1252.0 73 1002.0 74 798.5 75 595.0 76 450.0 77 305.0 78 227.5 79 115.5 80 81.0 81 58.0 82 35.0 83 22.0 84 9.0 85 5.5 86 2.0 87 1.5 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.027215441622877718 14 0.004884822855388308 15 0.24633463827886756 16 0.05512871508223949 17 0.0 18 0.0020934955094521323 19 0.0997899526172183 20 0.09071813874292572 21 0.0 22 0.0 23 0.0 24 0.001395663672968088 25 0.0034891591824202203 26 0.00837398203780853 27 0.0983942889442502 28 0.1311923852590003 29 0.0348915918242022 30 0.016050132239133014 31 0.030006768968813897 32 0.08373982037808529 33 0.16538614524671846 34 0.04396340569849478 35 0.04605690120794691 36 0.03768291917013838 37 0.18422760483178763 38 0.2784349027571336 39 0.2198170284924739 40 0.7676150201324484 41 0.933698997215651 42 0.12002707587525559 43 0.21074521461818133 44 0.5003454267590597 45 0.20795388727224512 46 0.1772492864669472 47 0.04047424651607456 48 0.15212734035352163 49 0.5198847181806129 50 0.1346815444414205 51 0.301463353361107 52 0.12002707587525559 53 0.16259481790078228 54 0.22051486032895795 55 0.23865848807754306 56 0.18004061381288336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 143301.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.72464117318513 #Duplication Level Percentage of deduplicated Percentage of total 1 85.48394784661167 61.31305485365495 2 8.31599492644732 11.929235041949243 3 2.8230585781321955 6.074485905822419 4 1.1789999790941266 3.3825340177487604 5 0.6409996194564545 2.2987733848831207 6 0.37999976833677135 1.6353208217909043 7 0.2849998261264293 1.430905718433681 8 0.18699988591224054 1.072999977318561 9 0.1369999164169425 0.8843642861185401 >10 0.5209996821403383 6.060011235535663 >50 0.03299997986685444 1.665445151960446 >100 0.012999992068760843 1.7579698826249153 >500 9.9999938990468E-4 0.49489972215878897 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 690 0.4815039671739904 No Hit ATTCCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 294 0.20516255992630897 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 290 0.20237123258037276 No Hit ATTCCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 286 0.1995799052344366 No Hit ATTCCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 217 0.15142950851703757 No Hit ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 205 0.14305552647922903 No Hit ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 196 0.13677503995087265 No Hit ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.12770322607658008 No Hit ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 164 0.11444442118338323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.005582654691872352 0.0 0.0 0.0 0.0 7 0.005582654691872352 0.0 0.0 0.0 0.0 8 0.005582654691872352 0.0 0.0 0.0 0.0 9 0.005582654691872352 0.0 0.0 0.0 0.0 10 0.009071813874292572 0.0 0.0 0.0 0.0 11 0.011165309383744704 0.0 0.0 0.0 0.0 12 0.014654468566164926 0.0 0.0 0.0 0.0 13 0.019539291421553234 0.0 0.0 0.0 0.0 14 0.021632786931005368 0.0 0.0 0.0 0.0 15 0.024424114276941543 0.0 0.0 0.0 0.0 16 0.028611105295845807 0.0 0.0 0.0 0.0 17 0.03140243264178198 0.0 0.0 0.0 0.0 18 0.03419375998771816 0.0 0.0 0.0 0.0 19 0.04047424651607456 0.0 0.0 0.0 0.0 20 0.04256774202552669 0.0 0.0 0.0 0.0 21 0.043265573862010735 0.0 0.0 0.0 0.0 22 0.047452564880914996 0.0 0.0 0.0 0.0 23 0.05094172406333522 6.97831836484044E-4 0.0 0.0 0.0 24 0.05373305140927139 6.97831836484044E-4 0.0 0.0 0.0 25 0.05931570610114375 6.97831836484044E-4 0.0 0.0 0.0 26 0.06699185630246823 6.97831836484044E-4 0.0 0.0 0.0 27 0.06978318364840441 6.97831836484044E-4 0.0 0.0 0.0 28 0.07397017466730867 6.97831836484044E-4 0.0 0.0 0.0 29 0.07745933384972889 6.97831836484044E-4 0.0 0.0 0.0 30 0.07815716568621293 6.97831836484044E-4 0.0 0.0 0.0 31 0.08164632486863316 6.97831836484044E-4 0.0 0.0 0.0 32 0.08443765221456934 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 33 0.08583331588753743 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 34 0.08792681139698956 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 35 0.08862464323347359 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 36 0.09211380241589381 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 37 0.09281163425237786 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 38 0.09420729792534595 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 39 0.09420729792534595 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 40 0.09490512976182999 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 41 0.09769645710776617 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 42 0.0997899526172183 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 43 0.0997899526172183 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 44 0.10676827098205874 6.97831836484044E-4 0.0 6.97831836484044E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGAT 15 0.008104482 50.479107 41 TATTTGC 20 3.9865775E-4 50.46124 40 CCCATGA 15 0.008115837 50.461235 40 AGCGTAG 20 4.0075247E-4 50.407707 37 TAAGCCC 15 0.008195655 50.33651 36 GTAAGCC 15 0.008218568 50.300987 35 TAAGACA 15 0.008218568 50.300987 35 TCCATAC 15 0.008287591 50.194717 44 CGATTCA 15 0.008287591 50.194717 49 CGATTAA 20 4.0921895E-4 50.194717 49 ACTATTA 30 1.0162694E-6 50.177048 47 CTCACTA 15 0.0082991365 50.177048 45 ACGATTA 25 2.0394073E-5 50.159393 48 TCGACCA 15 0.008310693 50.159393 48 TGTAGCG 15 0.008322263 50.14175 42 CACCATA 15 0.00838029 50.05372 33 CATATAC 15 0.0084737595 49.913513 28 ATATACT 15 0.0084737595 49.913513 29 CACCGTA 20 4.2072803E-4 49.913513 28 CCACCGT 20 4.2072803E-4 49.913513 27 >>END_MODULE