FastQCFastQC Report
Fri 17 Jun 2016
SRR1526190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57940
Sequences flagged as poor quality0
Sequence length56
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7601.3117017604418364No Hit
CTGAAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2100.3624439074905074No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1930.33310321021746636No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1930.33310321021746636No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1860.32102174663444943No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1790.3089402830514325No Hit
CTGAAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1700.2934069727304108No Hit
CTGAAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1440.24853296513634793No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1370.23645150155333106No Hit
CTGAAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1330.22954780807732136No Hit
CTGAAAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGA960.16568864342423195No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC940.16223679668622712No Hit
CTGAAAGGGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCG890.15360717984121505No Hit
CTGAAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC830.14325163962720056No Hit
CTGAAAGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGA810.13979979288919572No Hit
CTGAAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC810.13979979288919572No Hit
CTGAAAGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG790.1363479461511909No Hit
CTGAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC790.1363479461511909No Hit
CTGAAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC780.1346220227821885No Hit
CTGAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC770.13289609941318606No Hit
CTGAAAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTCTCAACTTTCGATGGT760.13117017604418363No Hit
CTGAAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC720.12426648256817396No Hit
CTGAAAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT710.12254055919917156No Hit
CTGAAAGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT700.12081463583016915No Hit
CTGAAAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG680.11736278909216431No Hit
CTGAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC660.11391094235415947No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT650.11218501898515706No Hit
CTGAAAGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA650.11218501898515706No Hit
CTGAAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC600.10355540214014498No Hit
CTGAAAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT600.10355540214014498No Hit
CTGAAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCCCTAATACATGCCGAC600.10355540214014498No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC580.10010355540214014No Hit
CTGAAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC580.10010355540214014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACT150.00818136150.32024441
CCATCGC150.00818136150.32024440
CATCGCA150.00818136150.32024441
CTCTACT150.00818136150.32024441
CTAGAAA150.00826644950.1888649
TAGAGCA252.017087E-550.1888636
GACGCCC150.00826644950.1888636
ATGCCTG507.2759576E-1250.1888636
CGCCCCT252.017087E-550.1888637
CTCAACG850.050.18885844
CGATTCA402.5211193E-950.18885849
TGCTCGT204.0740398E-450.18885837
ATGCCCA204.0740398E-450.18885836
CATGCCC252.0275156E-550.14521835
TATGCCT507.2759576E-1250.14521835
TTATTCC204.0915943E-450.14521435
CAACGAT850.050.1016546
TAGAAAC150.0083235450.1016550
CACGATT150.0083235450.1016547
TTTCATC150.0083235450.1016546