FastQCFastQC Report
Fri 17 Jun 2016
SRR1526189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130316
Sequences flagged as poor quality0
Sequence length56
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8870.6806531814972835No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3630.27855366954172933No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2910.2233033549218822No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2860.21946652751772613No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2750.21102550722858285No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2680.20565394886276436No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2570.19721292857362102No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2270.17419196414868474No Hit
GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2120.1626814819362166No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1970.15117099972374842No Hit
GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG1810.13889315203044905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT150.00814771550.4086339
CGGTTGT150.008248450.25232336
TGATTTA150.00828639550.1939634
CGATTAA507.2759576E-1250.17453849
ACGATTA507.2759576E-1250.17453848
TCAATCG150.00829908950.17453848
TCAAGAA150.00829908950.17453848
CGATTCA355.0986273E-850.17453449
ACGATTC355.0986273E-850.17453448
CTGTCAG252.0393987E-550.1551345
ACTGTTA150.00841398550.00038550
TATAGAC150.00841398550.00038550
AATCGCA150.00841398550.00038550
GCATTAT150.00843967849.9618512
AGCATTA204.185811E-449.96184511
AGTTACG204.185811E-449.96184511
GTACCCC150.00845254649.9426049
AACAATT150.00846542849.92337428
CAATTTA150.00847832649.90415630
TAGAGTG150.00849123849.88495623
Produced by FastQC (version 0.11.5)