##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526189_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 130316 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.314896098713895 39.0 37.0 39.0 33.0 40.0 2 36.977462475827984 39.0 37.0 39.0 31.0 40.0 3 35.28740139353571 38.0 35.0 39.0 23.0 40.0 4 36.953935050185706 39.0 37.0 39.0 32.0 40.0 5 34.56552533840818 38.0 35.0 39.0 18.0 40.0 6 36.87685318763621 39.0 37.0 39.0 31.0 40.0 7 37.09633506246355 39.0 37.0 39.0 33.0 40.0 8 37.074825808035854 39.0 37.0 39.0 33.0 40.0 9 37.05904110009515 39.0 37.0 39.0 32.0 40.0 10 36.53700236348568 38.0 36.0 39.0 31.0 40.0 11 36.51417324043095 38.0 36.0 39.0 31.0 40.0 12 34.58389606801928 38.0 34.0 39.0 23.0 40.0 13 34.28371803922772 38.0 33.0 39.0 22.0 40.0 14 34.418129776850115 38.0 33.0 39.0 23.0 40.0 15 34.38865526873139 38.0 33.0 39.0 23.0 40.0 16 33.84907455723012 38.0 32.0 39.0 22.0 40.0 17 33.73558887626999 38.0 32.0 39.0 22.0 40.0 18 33.26276896160103 37.0 31.0 39.0 20.0 40.0 19 34.11615611283342 38.0 32.0 39.0 24.0 40.0 20 34.14277602136345 37.0 32.0 39.0 24.0 40.0 21 34.82599220356671 38.0 33.0 39.0 26.0 40.0 22 34.522836796709534 38.0 33.0 39.0 25.0 40.0 23 35.08983547684091 38.0 34.0 39.0 27.0 40.0 24 34.66489916817582 38.0 33.0 39.0 25.0 40.0 25 34.94634580558028 38.0 33.0 39.0 27.0 40.0 26 35.3945716565886 38.0 35.0 39.0 28.0 40.0 27 35.277226127259894 38.0 35.0 39.0 28.0 40.0 28 35.1962690690322 38.0 35.0 39.0 28.0 40.0 29 35.0082108106449 38.0 35.0 39.0 27.0 40.0 30 34.806631572485344 38.0 34.0 39.0 27.0 40.0 31 34.98431504957181 38.0 35.0 39.0 27.0 40.0 32 34.695340556800396 38.0 34.0 39.0 27.0 40.0 33 34.777249148224314 38.0 34.0 39.0 27.0 40.0 34 34.581425151171 38.0 33.0 39.0 27.0 40.0 35 34.415067988581605 38.0 33.0 39.0 26.0 40.0 36 34.064642868105224 37.0 33.0 38.0 27.0 40.0 37 33.79813683661254 37.0 33.0 38.0 26.0 40.0 38 33.5479143006231 36.0 33.0 38.0 25.0 40.0 39 32.948210503698704 36.0 32.0 38.0 22.0 39.0 40 29.95939869240922 35.0 26.0 38.0 10.0 39.0 41 27.016805304030203 32.0 17.0 38.0 6.0 39.0 42 30.57696675772737 35.0 28.0 38.0 10.0 39.0 43 29.166963381319256 33.0 26.0 38.0 5.0 39.0 44 29.15198440713343 34.0 26.0 38.0 4.0 39.0 45 30.162451272291968 34.0 28.0 38.0 4.0 39.0 46 29.61461370821695 35.0 27.0 38.0 4.0 39.0 47 29.210143036925626 34.0 26.0 38.0 4.0 39.0 48 29.174514257650635 34.0 26.0 38.0 4.0 39.0 49 26.88730470548513 33.0 16.0 38.0 4.0 39.0 50 28.80090702599834 34.0 25.0 38.0 4.0 39.0 51 29.061020903035697 35.0 26.0 38.0 4.0 40.0 52 28.780280241873598 35.0 25.0 38.0 4.0 40.0 53 28.354760735443076 34.0 23.0 38.0 4.0 40.0 54 27.423954080849626 33.0 18.0 38.0 4.0 39.0 55 27.70350532551644 34.0 22.0 38.0 4.0 39.0 56 26.475973786795176 33.0 16.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.1504614515670681 1 2 1.393999189321626 1 3 3.801574797727625 1 4 1.4705982072671517 1 5 4.735808297739204 1 6 1.4963014419946106 1 7 1.3747689357164674 1 8 1.3587713958817034 1 9 1.3670249011006632 1 10 1.3967328375462102 1 11 -2.738981224214868 1 12 -13.157291335560998 1 13 -6.921424293447 1 14 -5.809003618230548 1 15 -6.461849622373194 1 16 -24.38329204195597 1 17 -5.17859146432173 1 18 -14.0528260263141 1 19 -6.102672406842196 1 20 -6.59254864735853 1 21 -6.333307570080542 1 22 -3.316853662547203 1 23 -3.815725562699715 1 24 -4.448371136881345 1 25 -3.522268344781857 1 26 1.9904200717880798 1 27 2.1152155582554357 1 28 1.855872638648222 1 29 2.2722218421403966 1 30 2.1220417614611975 1 31 2.3619938853033347 1 32 2.361610557748051 1 33 1.8573625540550438 1 34 1.2797682572331013 1 35 1.9184881901492474 1 36 1.4180581712406095 1 37 1.8680411022283039 1 38 1.439889413470425 1 39 1.9840408756312868 1 40 4.166196688080742 1 41 -11.701071075977332 1 42 -11.153458207564398 1 43 -19.183355456670448 1 44 -22.59781541672848 1 45 -13.923238045780174 1 46 -19.085794715109632 1 47 -23.405012248314186 1 48 -17.154271306365736 1 49 -20.034718208749062 1 50 -16.529402371875406 1 51 -13.559265121758221 1 52 -13.256947420485249 1 53 -12.601851994265303 1 54 -11.970583971791958 1 55 -12.260842073500282 1 56 -8.73094432752653 2 1 1.9066761408326016 2 2 2.185171505705803 2 3 4.4852965491400525 2 4 2.3277662863632003 2 5 5.429628919208135 2 6 2.3992675436895254 2 7 2.197085319897255 2 8 2.291433825260235 2 9 2.2381407203097012 2 10 2.2569729505405647 2 11 0.5931868548811821 2 12 -3.3785130869734274 2 13 -1.927215253898975 2 14 2.4031432744248207 2 15 1.9445769312991246 2 16 2.3132828168010917 2 17 3.0955610780511478 2 18 -1.338030828573988 2 19 2.3256468021973546 2 20 2.4210106746753652 2 21 2.761395819749964 2 22 2.5430333431025147 2 23 2.59737471978616 2 24 1.6722503320452091 2 25 2.6382895648226636 2 26 2.8871361734829932 2 27 2.6896505865040226 2 28 2.670561904184943 2 29 2.7301667573946347 2 30 3.0117310269979143 2 31 2.026718461574518 2 32 2.5805724221548303 2 33 2.5681676388008086 2 34 2.381639726159655 2 35 2.393770676024957 2 36 2.487902804008968 2 37 2.763486017482542 2 38 2.9146422383291792 2 39 3.263666581845982 2 40 5.3814862361033455 2 41 -8.634157234169422 2 42 -7.304129111519202 2 43 -15.588087095088529 2 44 -15.834291405429044 2 45 -6.832312904537236 2 46 -10.888089912849747 2 47 -17.13393173983961 2 48 -8.119384300716021 2 49 -13.475184310443977 2 50 -8.065843049841508 2 51 -3.006016534187598 2 52 -2.9992849911067196 2 53 -2.9293237456777312 2 54 -1.6203721073851796 2 55 -2.594846310788416 2 56 0.5101573673887216 3 1 2.0364402875459007 3 2 2.3934039512263823 3 3 4.73513961254244 3 4 2.65760217552112 3 5 5.752111207792119 3 6 2.4990792197723906 3 7 2.3766207875683207 3 8 2.492402348262651 3 9 2.1365354831112455 3 10 2.318492096805471 3 11 1.780165601181956 3 12 4.107093585073919 3 13 2.4810889340662285 3 14 2.9547529955260643 3 15 3.486232388208819 3 16 4.064426212012286 3 17 4.352757742027478 3 18 4.6823260900880115 3 19 3.9532138365440446 3 20 3.882583627773741 3 21 3.3741659748930033 3 22 3.3572204115268747 3 23 3.296146130421981 3 24 3.4719992334890293 3 25 3.0345768933133854 3 26 2.537212399830409 3 27 2.8611150291549023 3 28 2.820952003727591 3 29 2.9088951225636777 3 30 2.8764068408262773 3 31 2.5976089646684173 3 32 2.7384954783829656 3 33 2.1936720778645693 3 34 2.408901855116696 3 35 1.2815834282444882 3 36 2.90965870034114 3 37 3.0215794620166676 3 38 2.4843669266979376 3 39 3.5344046322450495 3 40 6.770330285964338 3 41 6.854319135662877 3 42 3.8333473479911575 3 43 -1.7552469910619841 3 44 1.5242414615784021 3 45 3.8768611605690353 3 46 2.3508124277475098 3 47 -3.454979179531115 3 48 1.6524747253802943 3 49 3.969977558446704 3 50 3.205253712780678 3 51 4.272613185120083 3 52 4.5085618387740105 3 53 4.164748534835226 3 54 4.759773171065184 3 55 4.569085175176969 3 56 5.28479641321421 4 1 1.7493744950453234 4 2 2.2510644067129277 4 3 4.376846536858061 4 4 2.3306525550932378 4 5 5.206188732521817 4 6 2.3028231811250492 4 7 2.100855892238208 4 8 2.0291518306643113 4 9 2.00017707501371 4 10 1.9831458810244698 4 11 1.8623774714373056 4 12 3.8875456209607435 4 13 3.9492278804660472 4 14 3.395344207856404 4 15 3.0463025515398527 4 16 4.332197088478814 4 17 4.119234622634792 4 18 4.734402234555461 4 19 3.313088462723023 4 20 3.3357122222066877 4 21 2.720496777745545 4 22 3.136407207018017 4 23 2.4182418286046357 4 24 2.797281037031702 4 25 2.3725686117398865 4 26 1.9265613761359077 4 27 2.009780775646739 4 28 1.559133508213442 4 29 1.6339066247490948 4 30 2.3839982832003273 4 31 1.5834612766076859 4 32 2.095034470791525 4 33 1.0666016844898252 4 34 0.9686269528852733 4 35 1.1793577553666381 4 36 1.9512103451536547 4 37 1.6563563196196114 4 38 2.2809220221660738 4 39 2.7288778321530316 4 40 5.422446688080743 4 41 7.9578963153270195 4 42 4.669096140261686 4 43 6.192188021590418 4 44 5.68397806153239 4 45 5.116164128132873 4 46 4.9905639805425395 4 47 3.862107316903206 4 48 5.229424345808177 4 49 6.061477443424852 4 50 4.305380236820241 4 51 3.036387052154822 4 52 3.3797373621234463 4 53 3.010648005734698 4 54 4.421263854294999 4 55 2.554103578673633 4 56 3.4244904550821644 5 1 1.9504614515670653 5 2 2.256499189321623 5 3 4.22146116136399 5 4 2.3654845709035186 5 5 5.506831025011934 5 6 1.9372105329037055 5 7 2.1668143902619192 5 8 2.1991123049726085 5 9 2.10566126473703 5 10 2.008096473909845 5 11 1.8809051394215004 5 12 4.072822300802635 5 13 3.639939342916634 5 14 4.170541836314904 5 15 4.231332195808626 5 16 4.6513670489531265 5 17 4.498681262950996 5 18 4.997742155504078 5 19 3.9484639567941713 5 20 3.6358604435505626 5 21 3.079192429919459 5 22 3.0956463374527985 5 23 2.6655244373002844 5 24 3.3686743176641087 5 25 2.688527109763598 5 26 1.6807609808789863 5 27 2.248170103709981 5 28 1.671781729557317 5 29 2.3159718421403994 5 30 1.8879508523702881 5 31 1.1489257034851548 5 32 1.8462696486571417 5 33 1.493726190418677 5 34 1.2542000754149214 5 35 1.3730336446947007 5 36 1.937376353058788 5 37 2.604972920410127 5 38 1.851821231652245 5 39 2.8402908756312897 5 40 6.9281285062625635 5 41 7.352906196749942 5 42 4.5959736106174205 5 43 5.534826361511371 5 44 5.572070946907889 5 45 4.409148317856193 5 46 4.327273466708547 5 47 4.430783206231268 5 48 4.467319602725169 5 49 7.282327245796392 5 50 4.6944612644882255 5 51 4.079371241878146 5 52 4.158393488605661 5 53 3.135648005734698 5 54 4.5941887554807685 5 55 4.013589744681536 5 56 3.6804193088371058 6 1 2.047520275096474 6 2 2.2609109540275085 6 3 4.7144424447864495 6 4 2.5893482072671503 6 5 5.506764180092148 6 6 2.3330661478769628 6 7 2.2835924651282298 6 8 2.216491984116999 6 9 2.0898190187477255 6 10 2.1415857787226855 6 11 2.0488864228439567 6 12 4.080576311497822 6 13 4.447693353611825 6 14 4.470408146475336 6 15 4.34035625997975 6 16 4.710457958044032 6 17 4.611114418031207 6 18 5.218865150156489 6 19 3.9215922990401566 6 20 3.8898042938179387 6 21 3.0762512534488735 6 22 3.1066757492175014 6 23 2.7853773784767526 6 24 3.2839818042951237 6 25 3.0200110669828533 6 26 2.067993601199845 6 27 2.1843332053142603 6 28 2.3323432268835163 6 29 2.4284718421403966 6 30 2.7970417614611947 6 31 1.559420355891568 6 32 1.9766840871598106 6 33 1.1875096128785714 6 34 1.099253551350749 6 35 1.3905470136786562 6 36 1.8923228771229645 6 37 1.932379337522427 6 38 1.9064335311174858 6 39 2.8013202873959955 6 40 6.107740805727804 6 41 3.219149512257964 6 42 4.407203557141486 6 43 5.855615131564846 6 44 5.245934583271524 6 45 4.617570777749236 6 46 4.037367049596249 6 47 4.811531869332875 6 48 4.514846340693083 6 49 6.902781791250941 6 50 4.614715275183414 6 51 4.724558407653543 6 52 5.249302579514751 6 53 5.353295064558228 6 54 5.5463277929139245 6 55 5.4498196912056045 6 56 4.520526260708763 7 1 1.8986863036380726 7 2 2.2764696035228056 7 3 4.435117697135908 7 4 2.428993177681356 7 5 5.463360072887134 7 6 2.425443453828933 7 7 1.9863073972549259 7 8 1.8772995023905779 7 9 2.110737918852145 7 10 1.792960648197102 7 11 1.854177059808805 7 12 3.920186475089892 7 13 4.010972156257139 7 14 3.985966795970633 7 15 4.333638543307281 7 16 4.39033961484877 7 17 4.726290192483006 7 18 4.67243284942554 7 19 3.727579072447746 7 20 3.743546027197681 7 21 2.545168761280408 7 22 3.2358090593462876 7 23 2.6858646739866785 7 24 3.0247057861955753 7 25 2.240268637466663 7 26 2.197335752261452 7 27 2.1298605286696386 7 28 1.9233282599499972 7 29 2.095927463442173 7 30 2.489349453768888 7 31 1.713510157492685 7 32 2.084132747097158 7 33 1.7349513114514963 7 34 2.035870328239021 7 35 1.2860917404451087 7 36 1.930854029228776 7 37 1.9517319306306717 7 38 1.6634471057781184 7 39 2.6180275620218225 7 40 5.119192250210919 7 41 -1.4401613126637223 7 42 3.4434722658083814 7 43 4.743641584749668 7 44 4.101555885046668 7 45 3.0669616583618406 7 46 4.0363576517542725 7 47 4.162628284230195 7 48 4.176941711385744 7 49 6.528225578233187 7 50 4.386869817473702 7 51 3.6284567717329033 7 52 4.142793703775105 7 53 3.6060622069181285 7 54 4.332892359568987 7 55 2.8352008259080037 7 56 3.5618071517634107 8 1 2.0619072346995964 8 2 2.498969068839692 8 3 4.751650098932444 8 4 2.448384351845462 8 5 5.583022153160897 8 6 2.7032291528379844 8 7 2.331997851379114 8 8 2.395443082628688 8 9 2.162958636040422 8 10 1.8664617532088599 8 11 2.0391061251827267 8 12 4.186458664439002 8 13 3.9463467908903525 8 14 4.184369875745354 8 15 4.686493751120786 8 16 4.708048319489819 8 17 4.531950704352965 8 18 5.457490238746139 8 19 4.179707111230094 8 20 3.926728461075207 8 21 3.238830984136328 8 22 3.0903752531154467 8 23 2.9704943168183533 8 24 3.4690987426367244 8 25 3.4290117757000758 8 26 2.022423083836273 8 27 2.3721432690988067 8 28 1.4618967350337684 8 29 2.9959417216584683 8 30 2.2608971831479394 8 31 2.096557138315383 8 32 2.2094268228082896 8 33 2.3204649636935955 8 34 2.142343558437922 8 35 2.567584575691413 8 36 2.434775038710491 8 37 1.830465801023486 8 38 1.9530671243137974 8 39 2.5548993093662276 8 40 4.5278683748277295 8 41 -2.3031042085074525 8 42 3.386225527375359 8 43 4.887578278269309 8 44 4.236296029054653 8 45 3.182560146990909 8 46 4.6115697427216915 8 47 4.717201607107505 8 48 4.9460298984535385 8 49 6.907601068359373 8 50 5.060356664269172 8 51 4.929590299928524 8 52 4.725206193972586 8 53 4.6958889695901185 8 54 5.213301570376718 8 55 5.1637814204756225 8 56 4.670561696569855 9 1 1.565241325780903 9 2 1.9648325226549588 9 3 4.21592228200435 9 4 2.249096634940109 9 5 5.313756410946752 9 6 2.211867479730458 9 7 1.7197374891755857 9 8 1.816750955630127 9 9 1.9130154671384005 9 10 2.0214969884896092 9 11 1.9233222349046315 9 12 4.010358035508183 9 13 3.9705568386284753 9 14 3.9259020421468094 9 15 4.0039522644192616 9 16 4.407942234773593 9 17 4.16527646020657 9 18 4.886678690667033 9 19 3.747563442214407 9 20 3.6577658180502723 9 21 1.8166138135672583 9 22 2.5913224380817326 9 23 2.382898651136763 9 24 2.7620062216092194 9 25 2.664406183520029 9 26 1.7940757321654388 9 27 1.9814105268088937 9 28 1.927570751855768 9 29 1.7982831628951175 9 30 2.451129811775658 9 31 1.1345960865611957 9 32 1.968803953974465 9 33 1.5566550068852294 9 34 1.5154207729563751 9 35 1.666129699583209 9 36 1.7138915045739438 9 37 1.8756275802157276 9 38 2.04661111158363 9 39 3.123702825316826 9 40 4.235339769841747 9 41 1.5734965340855602 9 42 3.4601817295425192 9 43 4.77285523515345 9 44 4.444676721636302 9 45 1.9346628976160538 9 46 4.082169121368352 9 47 3.5579987579751204 9 48 3.7590934735084787 9 49 5.800266067980498 9 50 3.3092768734076117 9 51 3.009760035474482 9 52 2.9537050952380213 9 53 2.8306165591938175 9 54 3.950249361541374 9 55 2.6411626434808504 9 56 2.411980200775357 10 1 1.5944008455064562 10 2 2.158771916594347 10 3 3.9688096462124705 10 4 2.3317724496913925 10 5 5.1814522371331435 10 6 2.2667559874491587 10 7 1.9611325720801034 10 8 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1.9815277953653165 115 27 2.186963525735109 115 28 2.203840118323022 115 29 2.3926994844168235 115 30 2.4149279403229826 115 31 1.5914146170106491 115 32 1.4357467366098362 115 33 1.4666105215347187 115 34 1.258172728777815 115 35 1.612593881206159 115 36 2.502509390752806 115 37 2.4016285006022926 115 38 2.621545917535464 115 39 3.533888436606901 115 40 6.53830034661733 115 41 6.1060935581690075 115 42 2.4241332558502364 115 43 -5.789961147727361 115 44 -5.370325579330103 115 45 1.6136013851141335 115 46 -3.3367601622641025 115 47 -2.905876069452397 115 48 -2.8914664283169564 115 49 -13.837868615253127 115 50 -2.6273698515502026 115 51 -1.8147529266362703 115 52 -1.311673030241348 115 53 -2.4537828885742456 115 54 -7.700157142523665 115 55 -2.427559553175076 115 56 -1.2689524576078313 116 1 1.3272220149473455 116 2 1.7934710203075355 116 3 3.6949726850515674 116 4 1.8372355312108155 116 5 4.940649847034983 116 6 1.89735778002278 116 7 1.5458956962798496 116 8 1.4203030860225496 116 9 1.5122713799739032 116 10 1.5944441051518439 116 11 -2.481850942524723 116 12 -13.369879363730014 116 13 -10.063325701897703 116 14 -4.320975449216466 116 15 -7.33420877730277 116 16 -3.950105422237659 116 17 -1.994436534744267 116 18 -8.455202786877482 116 19 -2.6563695899407875 116 20 -3.371421886795151 116 21 2.019333274989883 116 22 1.9451181684387109 116 23 1.757954014765076 116 24 1.5030373138228796 116 25 1.5640872890209607 116 26 1.1669165506613197 116 27 1.545145135720226 116 28 1.8079853147045597 116 29 1.691852123830543 116 30 1.7547882403344346 116 31 1.088666420514599 116 32 1.7051845014100167 116 33 1.5149329765902522 116 34 1.184257693852821 116 35 0.9308121338112159 116 36 1.9228116923673682 116 37 2.1265798346226745 116 38 2.055646455723945 116 39 2.665114819293258 116 40 5.0738551387849675 116 41 3.600425402895908 116 42 -0.9968560948883436 116 43 -6.622263907374673 116 44 -6.248431613911578 116 45 -0.5689246654984821 116 46 -5.038523588349069 116 47 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1.1184718421404014 117 30 1.5470417614611947 117 31 1.628243885303334 117 32 1.8978605577480465 117 33 1.6798625540550418 117 34 1.5710182572330993 117 35 1.7534881901492483 117 36 2.070558171240606 117 37 2.3017911022283073 117 38 2.1011394134704275 117 39 3.2677908756312917 117 40 6.072446688080742 117 41 3.6426789240226682 117 42 -1.1922082075643985 117 43 -8.440855456670448 117 44 -8.514065416728478 117 45 -0.5894880457801754 117 46 -3.8220447151096337 117 47 -4.393762248314186 117 48 -4.9192713063657365 117 49 -7.027218208749062 117 50 -3.869402371875406 117 51 -3.3942651217582203 117 52 -3.1506974204852476 117 53 -3.2643519942653008 117 54 -11.393083971791958 117 55 -3.5995920735002827 117 56 -3.325944327526532 118 1 0.903164154269767 118 2 1.1720397298621634 118 3 3.6680725454753684 118 4 1.5542130721320149 118 5 4.4546709103518225 118 6 1.3699500906432576 118 7 1.2004446113921432 118 8 1.1075889634492668 118 9 1.1491194956952597 118 10 1.2484220267353976 118 11 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0.2868389266256486 119 32 -4.5788336571279835 119 33 -0.4954267021433054 119 34 0.30688602582814184 119 35 0.20877744634759665 119 36 0.50452511338937 119 37 0.6865844906580563 119 38 0.6736187523134021 119 39 1.3728321979453426 119 40 4.354264869898923 119 41 1.4112739653449822 119 42 -2.317249529878449 119 43 -10.402673638488633 119 44 -9.50282574730699 119 45 -7.825355814375214 119 46 -17.618490996101368 119 47 -15.292357289636499 119 48 -15.288362215456646 119 49 -15.36746614263336 119 50 -13.87113790906549 119 51 -11.207818840766485 119 52 -11.106069321311697 119 53 -11.347823068645468 119 54 -12.903745128816752 119 55 -10.547360668541604 119 56 -7.786522839923226 120 1 0.2492419393719416 120 2 0.6558894332240612 120 3 2.9813004074837224 120 4 0.8234945487305652 120 5 3.9152290294465217 120 6 0.7700819297994883 120 7 0.7076957649847557 120 8 0.45465554222316484 120 9 0.6642809986616385 120 10 0.6324035692535261 120 11 -3.728158053483156 120 12 -13.423297433121974 120 13 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120 51 -11.168411463221634 120 52 -11.262892542436468 120 53 -11.852156872314083 120 54 -13.163571776670008 120 55 -11.606421341792965 120 56 -8.651066278746043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 675.0 5 23.0 6 88.0 7 100.0 8 216.0 9 204.0 10 175.0 11 127.0 12 168.0 13 181.0 14 254.0 15 274.0 16 407.0 17 564.0 18 641.0 19 874.0 20 1047.0 21 1320.0 22 1649.0 23 1985.0 24 2370.0 25 2740.0 26 2951.0 27 3186.0 28 3776.0 29 4191.0 30 4997.0 31 5859.0 32 6810.0 33 7892.0 34 9189.0 35 11141.0 36 13658.0 37 16975.0 38 15125.0 39 8484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.42601062033826 0.4888118112894809 0.04067037048405415 0.0445071978882102 8 99.52346603640382 0.4020995119555542 0.0445071978882102 0.0299272537524172 9 98.22585100831824 1.0466865158537708 0.29390097915835356 0.43356149666963384 10 56.70600693698395 29.220510144571655 7.187912458945947 6.8855704594984495 11 38.87857208631327 26.871604407747324 17.623315632769575 16.62650787316983 12 33.86997759292796 14.32364406519537 23.189784830719177 28.616593511157497 13 25.704001043817303 14.02629498583939 26.778518853950008 33.491185116393304 14 23.561957088922313 16.046379569661436 31.25786549617852 29.13379784523773 15 28.45453426848776 16.313676633444075 28.251507321274765 26.9802817767934 16 34.042079397988175 18.327574291637873 23.698840512938645 23.931505797435307 17 30.709199177384207 20.103440866816047 24.844992172872097 24.342367782927653 18 29.327624046533757 21.09520082262842 25.104746995718035 24.472428135119788 19 32.12439213637656 22.918667271009227 20.690794275134632 24.266146317479585 20 29.53070643617961 25.297004231398358 22.01555864441663 23.156730688005407 21 29.53896681911661 21.03578992602597 26.69280825071365 22.732435004143774 22 28.4569814911446 20.056631572485344 25.605451364375824 25.88093557199423 23 28.74551091193714 20.262285521348108 25.694465760152248 25.29773780656251 24 29.455323981706005 20.647502992725375 25.88247030295589 24.01470272261273 25 29.528362698753764 20.501565473632514 24.326232426791087 25.643839400822642 26 24.774183646061164 19.817351598173516 26.41187982042132 28.996584935344 27 24.985404375614554 18.00101401179941 27.3491273352999 29.664454277286133 28 24.601198709082524 22.990625480252035 26.595205163669895 25.81297064699554 29 29.46915902199363 20.641768702260777 26.06225770544659 23.826814570299007 30 27.0852520415055 18.60072450420581 30.922668385829187 23.391355068459507 31 27.977556549971982 20.771704673671927 28.897860810696713 22.352877965659374 32 32.99874047677562 17.823021626935365 27.909959449496192 21.268278446792824 33 29.485918091255687 17.817611609888083 30.19231951789448 22.50415078096175 34 24.536834427425237 21.76743828937771 34.589427617183 19.106299666014053 35 23.698898314843962 23.278952823308128 35.25622816782465 17.76592069402326 36 24.460326721120186 26.090093963028927 29.742676410980778 19.70690290487011 37 26.50296749592546 23.275623481656876 30.52446262185184 19.696946400565825 38 29.236084925624667 23.13830810548754 28.528884408498715 19.096722560389075 39 26.85494164142152 20.94065724522905 28.29429946639192 23.91010164695751 40 19.71944197549948 15.770761680256598 27.453723383699813 37.056072960544114 41 16.126909918417265 15.456600151709832 26.72724739538988 41.68924253448302 42 23.75189223664292 19.267387445538166 31.287796706547716 25.6929236112712 43 19.799855391289633 15.866190791194251 33.06539698167777 31.268556835838346 44 21.478368165342793 16.12246471813064 30.67864579316727 31.720521323359296 45 24.765075898555853 19.524461327878686 32.71635317820397 22.994109595361497 46 22.956680865460026 21.259745651939905 30.802411231911915 24.981162250688154 47 21.30447800152005 21.22079856286322 32.81922938146308 24.65549405415365 48 23.14982053508981 20.88556517996449 34.33659470136578 21.62801958357992 49 20.349927098521142 17.289607874902604 28.83349919384079 33.52696583273546 50 23.772167071334174 19.158189138519223 32.692780526784894 24.376863263361713 51 21.832259653497733 22.20554619132282 32.859990610111836 23.102203545067614 52 22.002919483712354 20.059926244622 34.57052858020898 23.36662569145667 53 21.68796027243516 20.085943145303876 35.5042049105976 22.72189167166336 54 20.626788516569743 20.12292685928798 31.796362965014307 27.45392165912797 55 22.119230769230768 21.728461538461538 31.223076923076924 24.92923076923077 56 24.064121785261218 21.002575635259294 29.90043439818552 25.032868181293967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 21.0 9 37.0 10 72.0 11 107.0 12 143.5 13 242.0 14 304.0 15 449.5 16 595.0 17 705.5 18 816.0 19 847.0 20 878.0 21 996.0 22 1280.0 23 1446.0 24 1738.0 25 2030.0 26 2291.5 27 2553.0 28 2813.0 29 3064.5 30 3056.0 31 2753.0 32 2450.0 33 2220.0 34 1990.0 35 1898.0 36 1806.0 37 1784.0 38 1869.0 39 1976.0 40 1990.0 41 2004.0 42 2342.0 43 2680.0 44 3286.5 45 3893.0 46 4041.5 47 4043.0 48 3896.0 49 4051.0 50 4206.0 51 4702.5 52 5199.0 53 5615.0 54 6601.0 55 7171.0 56 8040.0 57 8909.0 58 10359.5 59 11810.0 60 11401.0 61 10992.0 62 9798.0 63 7830.0 64 7056.0 65 6206.0 66 5356.0 67 4659.5 68 3963.0 69 3499.0 70 3035.0 71 2572.0 72 1783.5 73 1458.0 74 1161.0 75 864.0 76 631.0 77 398.0 78 291.0 79 133.0 80 82.0 81 56.0 82 30.0 83 20.0 84 10.0 85 9.0 86 8.0 87 4.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.019184137020780256 14 0.0 15 0.2179317965560637 16 0.06599343135148408 17 0.0 18 0.0015347309616624207 19 0.11280272568218791 20 0.07596918260228981 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0030694619233248414 26 0.008441020289143314 27 0.10743116731636944 28 0.13505632462629302 29 0.03913563952239173 30 0.015347309616624207 31 0.02532306086742994 32 0.08287547192977071 33 0.16882040578286628 34 0.054482949139015927 35 0.04680929433070383 36 0.039903005003222934 37 0.18416771539949045 38 0.2816231314650542 39 0.19798029405445225 40 0.7144172626538567 41 0.860984069492618 42 0.13735842106878665 43 0.23788329905767516 44 0.49571810061696187 45 0.21025814174775162 46 0.19798029405445225 47 0.043739832407378984 48 0.15884465453206054 49 0.5287148162927039 50 0.12891740077964334 51 0.2985051720433408 52 0.1197090150096688 53 0.17649406059117836 54 0.2455569538659873 55 0.24248749194266248 56 0.1926087356886338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 130316.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.31587832652936 #Duplication Level Percentage of deduplicated Percentage of total 1 85.93225445467849 61.283342030142116 2 8.198114831712147 11.693115196905984 3 2.583498321425497 5.527333558427207 4 1.1610140311612294 3.3119494152675037 5 0.6014891968666609 2.1447865189232327 6 0.39812343978651976 1.7035513674452867 7 0.2496341568391151 1.2462015408698857 8 0.16247740380476888 0.9269775008441019 9 0.1269690970129982 0.8149421406427453 >10 0.5326246018765602 6.537953896681911 >50 0.03550830679177068 1.645998956382946 >100 0.017216148747525178 2.4831946959697966 >500 0.0010760092967203236 0.6806531814972835 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 887 0.6806531814972835 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 363 0.27855366954172933 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 291 0.2233033549218822 No Hit GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 286 0.21946652751772613 No Hit GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 275 0.21102550722858285 No Hit GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 268 0.20565394886276436 No Hit GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 257 0.19721292857362102 No Hit GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 227 0.17419196414868474 No Hit GTATACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 212 0.1626814819362166 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 197 0.15117099972374842 No Hit GTATACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG 181 0.13889315203044905 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 7.673654808312102E-4 0.0 0.0 0.0 0.0 11 0.0023020964424936307 0.0 0.0 0.0 0.0 12 0.003069461923324841 0.0 0.0 0.0 0.0 13 0.003069461923324841 0.0 0.0 0.0 0.0 14 0.005371558365818472 0.0 0.0 0.0 0.0 15 0.005371558365818472 0.0 0.0 0.0 0.0 16 0.006138923846649682 0.0 0.0 0.0 0.0 17 0.007673654808312103 0.0 0.0 0.0 0.0 18 0.008441020289143314 0.0 0.0 0.0 0.0 19 0.010743116731636944 0.0 0.0 0.0 0.0 20 0.011510482212468155 0.0 0.0 0.0 0.0 21 0.013045213174130574 0.0 0.0 0.0 0.0 22 0.014579944135792996 0.0 0.0 0.0 0.0 23 0.017649406059117835 0.0 0.0 0.0 0.0 24 0.019951502501611467 0.0 0.0 0.0 0.0 25 0.02302096442493631 0.0 0.0 0.0 0.0 26 0.02839252279075478 0.0 0.0 0.0 0.0 27 0.030694619233248413 0.0 0.0 0.0 0.0 28 0.033764081156573256 0.0 0.0 0.0 0.0 29 0.03836827404156051 0.0 0.0 0.0 0.0 30 0.03913563952239173 0.0 0.0 0.0 0.0 31 0.039903005003222934 0.0 0.0 0.0 0.0 32 0.041437735964885355 0.0 0.0 0.0 0.0 33 0.041437735964885355 0.0 0.0 0.0 0.0 34 0.04220510144571656 0.0 0.0 0.0 0.0 35 0.04297246692654778 0.0 0.0 0.0 0.0 36 0.0445071978882102 0.0 0.0 0.0 0.0 37 0.04757665981153504 0.0 0.0 0.0 0.0 38 0.04834402529236625 0.0 0.0 0.0 0.0 39 0.049111390773197455 0.0 0.0 0.0 0.0 40 0.04987875625402867 0.0 0.0 0.0 0.0 41 0.05294821817735351 0.0 0.0 0.0 0.0 42 0.05601768010067835 0.0 0.0 0.0 0.0 43 0.05831977654317198 0.0 0.0 0.0 0.0 44 0.05908714202400319 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCTT 15 0.008147715 50.40863 39 CGGTTGT 15 0.0082484 50.252323 36 TGATTTA 15 0.008286395 50.19396 34 CGATTAA 50 7.2759576E-12 50.174538 49 ACGATTA 50 7.2759576E-12 50.174538 48 TCAATCG 15 0.008299089 50.174538 48 TCAAGAA 15 0.008299089 50.174538 48 CGATTCA 35 5.0986273E-8 50.174534 49 ACGATTC 35 5.0986273E-8 50.174534 48 CTGTCAG 25 2.0393987E-5 50.15513 45 ACTGTTA 15 0.008413985 50.000385 50 TATAGAC 15 0.008413985 50.000385 50 AATCGCA 15 0.008413985 50.000385 50 GCATTAT 15 0.008439678 49.96185 12 AGCATTA 20 4.185811E-4 49.961845 11 AGTTACG 20 4.185811E-4 49.961845 11 GTACCCC 15 0.008452546 49.942604 9 AACAATT 15 0.008465428 49.923374 28 CAATTTA 15 0.008478326 49.904156 30 TAGAGTG 15 0.008491238 49.884956 23 >>END_MODULE