FastQCFastQC Report
Fri 17 Jun 2016
SRR1526188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154140
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG19031.2345919294148178No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG5530.35876475930971846No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG5070.3289217594394706No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4380.2841572596340989No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3630.23550019462826002No Hit
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG3200.20760347735824577No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3160.2050084338912677No Hit
GAATTAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG2920.18943817308939925No Hit
GAATTAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG2860.18554560788893215No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2850.18489684702218762No Hit
GAATTAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2850.18489684702218762No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2680.17386791228753082No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2340.15181004281821722No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2130.13818606461658234No Hit
GAATTAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1700.11028934734656805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAAT150.00816164650.39133541
AACACCA150.00816164650.39133539
ACACCGA150.00821475350.3087537
CGAAACA150.00823606850.27579544
TCTCGTG204.0606983E-450.27579544
CCGAAAC150.00823606850.27579543
CGACTCC355.0264134E-850.27579544
CTTAACG150.00826811750.22644445
CGATTCA252.0240806E-550.2264449
ACGCCAT150.00827882150.2100148
TAAACCG150.00827882150.2100136
CAAGATT150.00827882150.2100147
AAGATTC150.00827882150.2100148
ACGATTC204.0870326E-450.21000748
GTCTGCA150.00828953550.19359235
CAACGAT1000.050.19359246
TTTAATC150.00833249350.1280234
AGTAGTG204.120141E-450.1280242
GTAAGAC204.120141E-450.1280234
GCGTGTT204.146781E-450.06262250
Produced by FastQC (version 0.11.5)