FastQCFastQC Report
Fri 17 Jun 2016
SRR1526187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15549
Sequences flagged as poor quality0
Sequence length56
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.3537204964949514No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG460.2958389607048685Illumina Single End Adapter 2 (97% over 34bp)
AGTCGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.25081998842369285No Hit
AGTCGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.21866357965142452No Hit
AGTCGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA340.21866357965142452No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.18650717087915622No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA250.16078204386134157No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA250.16078204386134157No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTCTGCCGTCTTCTGCTTG240.1543507621068879Illumina Single End Adapter 1 (96% over 33bp)
AGTCGTGGGCTCGTCTTAATCAGCAACATCTTTTGATACACAAAAAAAAAAAAAAA210.13505691684352691No Hit
AGTCGTGGGCAGTGGCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.13505691684352691No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTACGCCGTCTTCTGCTTG210.13505691684352691Illumina Single End Adapter 1 (96% over 33bp)
AGTCGTGGGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.12219435333461959No Hit
AGTCGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG180.11576307158016592No Hit
AGTCGTGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA170.10933178982571226No Hit
AGTCGTGGGACACGCAGGGATGAGATGGGCAAAAAAAAAACAAAAAAAAAAAAAAA170.10933178982571226No Hit
AGTCGTGGGAAGCAGTTATCACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT170.10933178982571226No Hit
AGTCGTGGGTACGAGACTCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10933178982571226No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGATATCGTATGCCGTCTTCTGCTTG160.10290050807125861RNA PCR Primer, Index 47 (96% over 28bp)
AGTCGTGGGCTCGTCTTAATCAGCAACATCTTTTGATACAAAAAAAAAAAAAAAAA160.10290050807125861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA150.00804002250.3648234
AGCTCGT150.0081445550.20129833
CGGCCGG203.9760233E-450.20129833
GTGCAAT150.00825008650.03883449
TGCAATC150.00825008650.03883450
TGCGTGC150.00825008650.03883446
CCAAAAC309.764935E-750.03883443
GCGGCCG150.00825008650.03883432
GAGCTCG150.00825008650.03883432
GCGTGCA150.00825008650.03883447
AGAGCTC150.00825008650.03883431
AGCGGCC150.00825008650.03883431
AAGCGGC150.00825008650.03883430
CGTGCAA150.00825008650.03883448
TGGGTGG550.049.8774226
TGGGAGC550.049.8774226
GGAGATG150.008356634549.877428
GGATGTC150.008356634549.877428
GGAGAGT204.1059035E-449.877428
GTCGTAT309.987143E-749.877422
Produced by FastQC (version 0.11.5)