FastQCFastQC Report
Fri 17 Jun 2016
SRR1526186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33679
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG840.24941358116333617No Hit
AGGGTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.17815255797381158No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAA550.16330651147599393No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAAAAAAAAAAAAAAAAAA510.15142967427773985No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACAAAAAAAAAAAAAAA500.1484604649781763No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT490.1454912556786128No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC440.13064520918079514No Hit
AGGGTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.13064520918079514No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAACAAAAAAAAAAAAAAA440.13064520918079514No Hit
AGGGTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.11579916268297753No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAACCAAAAAAAAAAAAAAA380.112829953383414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAC150.00828661650.11194243
CACCACA204.0802706E-450.1119444
CAGTCCC150.00833571650.0372614
TCACTCA150.00833571650.0372633
CTACTCA150.00833571650.0372633
TCTTAAT301.0122785E-650.0372614
AAACCTT150.00838503249.962825
TTTGAAC150.00838503249.962829
GGCACTC150.00838503249.962828
TGCATAT150.00838503249.962816
TATATGA150.00838503249.962820
GAGTGCC150.00838503249.96289
CCTGCTA150.00838503249.962849
GATATGG150.00838503249.962850
TGGTACG150.00838503249.962810
ACCACAA150.00838503249.962845
GTTCTAC150.00838503249.962830
GAAGTGT204.1408988E-449.96289
GAAGCCT150.00838503249.96289
GAAGCAA204.1408988E-449.96289
Produced by FastQC (version 0.11.5)